KR100354606B1 - Composition of multi-purpose solution for treating contact lens - Google Patents

Abstract

The present invention relates to a multi-purpose contact lens care solution composition, specifically 0.005 to 10% by weight of a polysaccharide having a molecular weight of 5,000 or more as a non-enzymatic protein remover; 0.01-10% by weight of nonionic surfactant; And it relates to a multi-purpose contact lens care solution composition comprising 0.00001 to 1% by weight of a polymeric preservative, the multi-purpose contact lens care solution composition according to the present invention, washing, disinfecting, preserving and removing proteins without a separate device or device It can be treated all at once and there is no side effect caused by the control solution residue.

Description

Composition of multi-purpose solution for treating contact lens

The present invention relates to a multi-purpose contact lens care solution composition, specifically 0.005 to 10% by weight of a polysaccharide having a molecular weight of 5,000 or more as a non-enzymatic protein remover; 0.01-10% by weight of nonionic surfactant; And 0.00001 to 1% by weight of a polymeric preservative.

Since contact lenses are preferred over aesthetic glasses as well as the ability to correct vision, the frequency of their use is increasing. On the other hand, contact lenses are prone to deposits on the surface due to external factors such as mascara and hair spray, as well as internal factors such as proteins, mucus, and fatty secretions in tear solutions. If deposits form on the contact lens, it will not only shorten the life of the lens but also shorten the wearing time, blur the field of vision, conjunctivitis due to eye irritation, etc., causing inconvenience to the user. Regular cleaning and disinfection are required to remove contaminants.

Conventionally, the cleaning, disinfection, and preservation of the lens had to be performed by separate operations, which was cumbersome and time consuming, causing inconvenience to lens users. Recently, a multi-purpose contact lens care solution (Multi-Purpose Solution) capable of cleaning, disinfecting, preserving, and removing proteins at once has been developed in various ways, and its use has been steadily increasing.

On the other hand, contact lenses are classified into hard contact lenses, soft contact lenses, and RGP contact lenses according to the characteristics of their materials. In particular, the most popular soft contacts are currently used. Lenses are further classified into four groups depending on the water content and ionicity of the material. Group I is non-ionic on the surface of the lens and has a moisture content of less than 50%, group II is nonionic and has a water content of at least 50%, group III is ionic on the surface of the lens and has a water content of less than 50%, And group IV has ionicity and water content is more than 50%. Depending on the characteristics of these different materials, the amount or amount of contaminants (eg, proteins) deposited or bound when the lens is worn, or the cleanliness of the lens after cleaning is changed. In particular, in case of group IV, due to the ionicity of the lens surface, it is reported that not only the protein deposition amount is high but also the protein binding strength is not removed well when washing (L. Minarik et al. , CLAO J , 15 ( 3), (1989), 185-188; GE Minno et al ., Optom. And Vis. Sci ., 68 (11), (1991) 865-872).

On the other hand, among the deposits attached to the lens, the fat component is easily removed by the general contact lens care solution, but the protein component is hardly combined with the material component of the contact lens and is not easily removed. It is important to remove protein effectively.

In general, the main protein components in human tear solution are lysozyme, albumin, lactoferrin, glycoprotein, mucin, and lysozyme. While it is easily removed by solution, it is reported that albumin, lactoferrin, and the like are not easily removed by general multipurpose lens care solution (L. Minarik et al ., CLAO J , 15 (3), (1989) 185 ~). 188; J. Jung et al ., CLAO J , 19 (1), (1993) 47-49). Therefore, in order to improve the protein removal ability of the multi-purpose contact lens care solution, it is necessary to easily remove proteins such as albumin and lactoferrin as well as lysozyme.

Accordingly, a multi-purpose contact lens using a protease has been developed. U. S. Patent No. 5,785, 767 discloses a composition containing proteolytic enzymes and polyhydric alcohols, such as glycerin or propylene glycol, which is used to complete disinfection and protein removal of the lens in a very fast time (about 5 minutes). Claimed to be able to.

Multipurpose contact lens care solutions also require that the lens be immersed in the solution for as little as 4 hours or as long as 8 hours during the disinfection and preservation process, during which time the components of the solution may be adsorbed or deposited on the lens. This increases the likelihood that the components will slowly release when the lens is worn again, causing a number of serious problems. Therefore, careful care must be taken in selecting the components of the lens care solution. Especially in the case of products containing proteolytic enzymes, even if a small amount of proteolytic enzymes remain on the surface of the lens after washing, it is highly likely to cause fatal damage to the eye when wearing the lens, so the lens should be thoroughly rinsed with a separate rinse solution. Therefore, efforts have been made to develop a multi-purpose contact lens care solution that does not contain proteolytic enzymes and exhibits excellent protein removal ability.

Multipurpose contact lens care solutions that do not currently contain proteolytic enzymes generally include low molecular weight additives, such as low molecular weight preservatives, low molecular weight disinfectants, low molecular weight surfactants, and the like. have. However, these low-molecular substances are known to be adsorbed on the surface or the pores of the lens to deform or discolor the lens to shorten the life of the lens, as well as to irritate the user's eyes and cause a sense of foreign object, thereby reducing the fit. Furthermore, these substances are expected to cause fatal eye damage (BJ Tripathi et al ., Lens and Eye Toxic. Res. , 3 (4 & 4), (1992), 361-375).

U.S. Patent No. 4,820,352 discloses a contact lens care solution composition using benzalkonium chloride, sorbic acid, chimerosal, etc. as a preservative, all of which are readily adsorbed on the lens surface causing irritation to the user's eyes when the lens is worn and the lens It has been found that there is a problem of shortening the life of the lens by discoloring (BJ Tripathi, et al., Lens and Eye Toxic. Res. , 3 (4 & 4), (1992), 361-375). In addition, the lens care solution typically uses a polymeric surfactant called poloxamin as a protein remover, and this component has a problem in that it does not exert sufficient effects due to its weak protein removal ability.

U.S. Patent No. 5,370,744 uses polycarboxylate, polysulfonate and polyphosphate with molecular weight of 90 to 600 as a component to remove proteins contaminated in contact lenses, and citrate. ), Tartrate and succinate are used alone or in combination. However, the salts are forbidden to be used in care solutions of lenses that come into direct contact with the body because they form complexes when mixed (Handbook of Pharmaceutical Excipients, 2nd Ed., 1994, American Pharmaceutical Association). In addition, the composition of the patent has the disadvantage that it does not have the proper viscosity required for the multipurpose lens care solution.

U.S. Patent No. 5,451,303 describes a method for removing proteins using an electrochemical potential difference, in which an oxidizing agent and a reducing agent are dissolved in a gel, respectively, and then coated with an oxide gel or a reducing gel on one surface of the contact lens and dissolved in a solvent. Another gel is added to remove the protein. However, in the above method, the lens must be coated and heated to 80 ° C. or higher to exhibit a cleaning effect. In addition, it is inefficient in terms of convenience and cost, such as separately storing the gel oxide and the reducing gel in separate containers.

U.S. Patent No. 5,858,937 proposes a multi-purpose lens care solution using phosphonate as a protein remover and said that it is possible to clean, disinfect, preserve and rinse hard, soft and RGP lenses, There is a problem that the protein removal ability is very low in the soft lens, especially in the group having high water content or ionic characteristics.

In US Pat. No. 5,897,833, a solution containing hydrogen peroxide and a solution containing a substance capable of decomposing hydrogen peroxide are contained in separate containers, first using a hydrogen peroxide solution to disinfect the lens, and then a solution of hydrogen peroxide decomposing material to the lens. A method of preventing side effects such as irritation and edema caused by hydrogen peroxide was suggested by removing the remaining hydrogen peroxide. However, this method is cumbersome and inefficient in terms of storing different solutions in separate containers and requiring two steps of cleaning.

International Patent Publication No. 95/01414 discloses a multipurpose lens care solution comprising a nonionic surfactant and quaternary ammonium phosphate ester. However, the poloxamer (polyoxypropylene-polyoxyethylene copolymer) used at this time is excellent in cleaning of non-protein deposits, such as lipids and inorganic substances, but the removal of proteins, which is the most problematic management of contact lenses, There is a weak problem.

As described above, commercially available multi-purpose contact lens care solution products contain a large amount of protease, and most of them do not include any protein degrading enzymes, even if they have no or no protein removal ability.

Accordingly, the present inventors have tried to develop a multi-purpose contact lens care solution that can effectively remove protein components as well as non-protein components deposited on the lens, ensures safety, and is easy to use for the user's convenience. The present invention is completed by using a polysaccharide having an molecular weight of 5,000 or more as an enzyme and including a polymeric nonionic surfactant and a polymeric preservative to prepare a multipurpose contact lens care solution composition and find that the composition has the above-mentioned excellent properties. It was.

It is an object of the present invention to provide a new multi-purpose contact lens care solution composition which effectively removes contaminants, especially protein components, deposited on the lens and ensures safety.

In order to achieve the above object, the present invention provides a multi-purpose contact lens care solution composition comprising a non-ionic surfactant and a polymeric preservative, using a polysaccharide having a molecular weight of 5,000 or more as a non-enzyme as a protein scavenger. do.

Hereinafter, the present invention will be described in detail.

0.005 to 10% by weight of a polysaccharide having a molecular weight of 5,000 or more as a non-enzyme protein removing agent in the present invention; 0.01-10% by weight of nonionic surfactant; And 0.00001 to 1% by weight of a polymeric preservative.

The multipurpose contact lens care solution composition according to the present invention comprises 0.005 to 10% by weight of a polysaccharide having a molecular weight of 5,000 or more; 0.01-10% by weight of nonionic surfactant; And 0.00001 to 1% by weight of a polymeric preservative, and may further include stabilizers, buffer solutions, and the like that are commonly used in contact lens care compositions.

1) Protein Remover

Protein remover used in the present invention is a polysaccharide having a molecular weight of 5,000 or more, hyaluronic acid (1 → 3) -O- (β-glucuronic acid)-(1 → 4) -O- (2-acetamido-2-deoxy -β-D-glucose)), sodium hyaluronate and chondroitin sulfate. Specifically, the polysaccharide is derived from a living body, hyaluronic acid and its sodium salt is a disaccharide of D-glucuronic acid (D-glucuronic acid) and N-acetyl-D-glucosamine (N-acetyl-D-glucosamine) It is a repeating polysaccharide. The chondroitin sulfate includes chondroitin-4-sulfate [(1 → 4) -O- (β-D-glucopyranosyl uronic acid)-(1 → 3) -O- (2-N-acetamido-2-deoxy-β-D -galctopyranosyl-4-sulfate] and chondroitin-6-sulfate [(1 → 4) -O- (β-D-glucopyranosyl uronic acid)-(1 → 3) -O- (2-N-acetamido-2-deoxy -β-D-galactopyranosyl-6-sulfate], and these include β-D-glucuronic uronic acid and α-DN-acetyl-galactosamine (α-DN-). acetyl-galactosamine) are alternately connected.

If the content of the polysaccharide is less than 0.005% by weight, the protein removal effect is insufficient, when the content of more than 10% by weight is too high viscosity of the solution is difficult to prepare a solution and is inconvenient for the user to handle. Therefore, the protein remover of the polysaccharide component as described above is preferably used in the range of 0.005 to 10% by weight, more preferably 0.01 to 5% by weight.

The polysaccharide is a non-enzymatic protein remover that removes lysozyme, albumin (mubu), mucin, and the like, which is a lacrimal protein that is adsorbed on the surface of the lens to blur the field of vision, congest the cornea, and cause foreign bodies. Of course, since it is not adsorbed by the lens during use, it does not cause deformation of the lens, and because it is a substance derived from a living body, there are no side effects such as irritation of the eye mucosa. In addition, the polysaccharides have non-Newtonian viscosity and dry eye and joint lubrication have been reported, so not only soft contact lenses but also hard contact lenses, RGP contact lenses, etc. It is also suitable as a multipurpose lens care solution (E. Gura et al ., Polymer Degradation and Stability , 59, (1998), 297-302).

2) nonionic surfactant

Nonionic surfactants used in the present invention are polyethylene oxide / polypropylene oxide and tetra (polyoxyethylene / polyoxypropylene) ethylenediamine block copolymers having a molecular weight of 5,000 or more. -[polyoxyethylene-co-polyoxypropylene] ethylenediamine blockcopolymer). Specifically, the polyethylene oxide / polypropylene oxide copolymer has an average molecular weight of 8,350 (ethylene oxide content: 82.0 wt%; poloxamer 188), 12,000 (ethylene oxide content: 73.0 wt%; poloxamer 338) and 15,000 (ethylene oxide content More preferably, the tetra (polyoxyethylene / polyoxypropylene) ethylenediamine block copolymer has an average molecular weight of 6,700 (ethylene oxide content: 60.0). More preferably selected from the group consisting of materials comprising wt%; Tetronic 904), 15,000 (ethylene oxide content: 70.0 wt%; Tetronic 1107) and 25,000 (ethylene oxide content: 60.0 wt%; Tetronic 908) .

If the content of the nonionic surfactant of the polymer is less than 0.01%, the action of preventing damage to the lens is weak, and the degree of foaming or the feeling of the lens is poor, and if it exceeds 10% by weight, the viscosity of the solution is too high. It is difficult to manufacture and inconvenient for users to handle. Therefore, it is preferable to use the nonionic surfactant which is the polymer in the range of 0.01 to 10% by weight, more preferably 0.1 to 5% by weight.

The nonionic surfactants prevent damage to the lens, generate adequate foam, and remove contaminants such as lipids and minerals. The above mentioned materials are also all characterized as hypoallergenic and water soluble to the eye and do not interact with other additives in the composition.

3) Polymeric Preservative

The polymeric preservative in the composition of the present invention is used for the purpose of disinfecting and preserving the lens, preferably a hydrolyzed collagen derivative or a water-soluble substance, and specifically, a hydrolyzed lauryldimonium hydroxypropyl collagen (lauryldimonium hydroxypropyl). hydrolyzed collagen, cocodimonium hydroxypropyl hydrolyzed collagen, hydrolyzed stearyldimonium hydroxypropyl hydrolyzed collagen, poly [oxyethylene (dimethylimino) ethylene Mino) ethylenedichloride] (poly [oxyethylene (dimethylimino) ethylene (dimethylimino) ethylene dichloride]), polyaminopropyl diguanide and polyquaternium-16 (polyquaternium-16) desirable.

If the content of the polymeric preservative is 0.00001% by weight or less, the disinfection and preservation effect is weak, and if it exceeds 1.0% by weight, irritation of the eye mucosa is induced. Therefore, it is preferable to use a polymeric preservative in the range of 0.00001 to 1.0 weight%, and it is more preferable to use 0.0001 to 0.1 weight% especially.

In general, sorbic acid, thimerosal, and benzalkonium chloride, which are used as preservatives in lens care solutions, are known to have problems in adsorbing the lens and irritating the eye mucosa. However, the polymeric preservative according to the present invention exhibits a strong disinfection and preservation effect without discoloring the lens or irritating the eye mucosa.

4) The composition of the present invention basically includes the protein remover, the nonionic surfactant, and the polymeric preservative, and may further include a stabilizer, a buffer solution, and the like that are commonly used in contact lens care solutions.

In this case, stabilizers include ethylenediamine tetraacetic acid disodium, ethylenediamine tetraacetic acid trisodium, and ethylenediamine tetraacetic acid tetrasodium. It is preferably selected from the group containing, preferably 0.01 to 0.5% by weight.

Stabilizers serve to increase the stability and increase the preservation of the main component. If the content of the stabilizer is less than 0.01% by weight, the function as a stabilizer is weak, and if it is 0.5% by weight or more, there is a problem such as causing eye irritation.

The buffer solution is preferably selected from the group comprising borate buffer, phosphate buffer and carbonate buffer solution. At this time, the buffer solution preferably has a pH of 6.0 to 8.0.

It is also desirable to make the contact lens care solution an isotonic solution using sodium chloride.

The multi-purpose contact lens care solution according to the present invention can be effectively used for all kinds of contact lenses including soft contact lenses, hard contact lenses, and RGP contact lenses (RGPid Contact Lenses), and the cleaning, rinsing, It can be used for various purposes including disinfection, preservation and protein removal.

Hereinafter, the present invention will be described in more detail with reference to Examples.

However, the following examples are illustrative of the present invention, and the content of the present invention is not limited by the examples.

Example 1 Preparation of Multipurpose Contact Lens Care Solution 1

After dissolving 0.058 g of disodium phosphate, 0.055 g of potassium hydrogen phosphate, 0.90 g of sodium chloride, and 0.125 g of disodium ethylenediamine tetraacetate dihydrogen stabilizer in distilled water completely (solution pH = 6.90), polyaminopropyldiguanide preservative 0.0001 g was added to dissolve. 1.0 g of polyethylene oxide / polypropylene oxide copolymer (average molecular weight; 8350, ethylene oxide content; 82.0 weight%: poloxamer 188), a nonionic surfactant, was slowly added to the solution to completely dissolve and 0.02 g of sodium hyaluronate, a protein remover, was added. The solution was added, mixed with stirring to completely dissolve, and then filtered through a 0.2 μm filter paper to prepare a multipurpose lens care solution.

Example 2 Preparation of Multipurpose Contact Lens Care Solution 2

Except for using 2.0 g of polyethylene oxide / polypropylene oxide copolymer (average molecular weight; 8350, ethylene oxide content; 82.0 weight%: poloxamer 188), which is a nonionic surfactant, and 0.1 g of sodium hyaluronate, a protein scavenger, A multipurpose lens care solution was prepared in the same manner as in Example 1.

Example 3 Preparation of Multipurpose Contact Lens Care Solution 3

Except for using 1.0 g of polyethylene oxide / polypropylene oxide copolymer (average molecular weight; 8350, ethylene oxide content; 82.0 weight%: poloxamer 188), which is a nonionic surfactant, and 0.02 g of chondroitin sulfate as a protein scavenger. A multipurpose lens care solution was prepared in the same manner as in Example 1.

Example 4 Preparation of Multi-Purpose Contact Lens Care Solution 4

Except for the use of polyethylene oxide / polypropylene oxide copolymer (average molecular weight; 8350, ethylene oxide content; 82.0 weight%: poloxamer 188), which is a nonionic surfactant, and 0.1 g of chondroitin sulfate as a protein remover. A multipurpose lens care solution was prepared in the same manner as in Example 1.

Example 5 Preparation of Multi-Purpose Contact Lens Care Solution 5

1.0 g of tetra (polyoxyethylene / polyoxypropylene) ethylenediamine block copolymer (average molecular weight; 15000, ethylene oxide content; 70 weight%: Tetronic 1107) as nonionic surfactant and 0.02 sodium hyaluronate as protein remover A multipurpose lens care solution was prepared by the same method as Example 1 except that g was used.

Example 6 Preparation of Multi-Purpose Contact Lens Care Solution 6

2.0 g of tetra (polyoxyethylene / polyoxypropylene) ethylenediamine block copolymer (average molecular weight; 15000, ethylene oxide content; 70 weight%: Tetronic 1107) as a nonionic surfactant and 0.1% sodium hyaluronate as a protein remover A multipurpose lens care solution was prepared by the same method as Example 1 except that g was used.

Example 7 Preparation of Multi-Purpose Contact Lens Care Solution 7

1.0 g of tetra (polyoxyethylene / polyoxypropylene) ethylenediamine block copolymer (average molecular weight; 15000, ethylene oxide content; 70 weight%: Tetronic 1107) as nonionic surfactant and 0.02 of chondroitin sulfate as protein remover A multipurpose lens care solution was prepared by the same method as Example 1 except that g was used.

Example 8 Preparation of Multi-Purpose Contact Lens Care Solution 8

As a nonionic surfactant, 2.0 g of tetra (polyoxyethylene / polyoxypropylene) ethylenediamine block copolymer (average molecular weight; 15000, ethylene oxide content; 70 wt%: Tetronic 1107) was used and 0.1% chondroitin sulfate was used as a protein remover. A multipurpose lens care solution was prepared by the same method as Example 1 except that g was used.

The composition of the multi-purpose contact lens care solution prepared in the above example is shown in Table 1 below.

Composition of Multi-Purpose Contact Lens Care Solution Ingredients and content (for 100 g total) Protein remover Surfactants Preservative Stabilizer Buffer solution NaCl Example 1 Sodium Hyaluronate0.02g Polyethylene oxide / polypropylene oxide copolymer (average molecular weight; 8350, ethylene oxide content; 82.0 wt%: poloxamer 188) 1.0 g Polyaminopropyldiguanide 0.0001 g Ethylenediamine tetraacetate disodium dihydrogen0.125g Disodium Phosphate 0.058g + Potassium Hydrogen Phosphate 0.055g 0.90 g Example 2 Sodium Hyaluronate0.1g Polyethylene oxide / polypropylene oxide copolymer (average molecular weight; 8350, ethylene oxide content; 82.0 wt%: poloxamer 188) 2.0 g Example 3 Chondroitin Sulfate0.02g Polyethylene oxide / polypropylene oxide copolymer (average molecular weight; 8350, ethylene oxide content; 82.0 wt%: poloxamer 188) 1.0 g Example 4 Chondroitin Sulfate0.1g Polyethylene oxide / polypropylene oxide copolymer (average molecular weight; 8350, ethylene oxide content; 82.0 wt%: poloxamer 188) 2.0 g

Composition of Multi-Purpose Contact Lens Care Solution Ingredients and content (for 100 g total) Protein remover Surfactants Preservative Stabilizer Buffer solution NaCl Example 5 Sodium Hyaluronate0.02g Tetra (polyoxyethylene / polyoxypropylene) ethylenediamine block copolymer (average molecular weight; 15000, ethylene oxide content; 70 wt%: Tetronic 1107) 1.0 g Polyaminopropyldiguanide 0.0001 g Ethylenediamine tetraacetate disodium dihydrogen0.125g Disodium Phosphate 0.058g + Potassium Hydrogen Phosphate 0.055g 0.90 g Example 6 Sodium Hyaluronate0.1g Tetra (polyoxyethylene / polyoxypropylene) ethylenediamine block copolymer (average molecular weight; 15000, ethylene oxide content; 70 wt%: Tetronic 1107) 2.0 g Example 7 Chondroitin Sulfate0.02g Tetra (polyoxyethylene / polyoxypropylene) ethylenediamine block copolymer (average molecular weight; 15000, ethylene oxide content; 70 wt%: Tetronic 1107) 1.0 g Example 8 Chondroitin Sulfate0.1g Tetra (polyoxyethylene / polyoxypropylene) ethylenediamine block copolymer (average molecular weight; 15000, ethylene oxide content; 70 wt%: Tetronic 1107) 2.0 g

<Manufacture example> Preparation of artificial tears

The phosphate buffer solution was filtered through a 0.45 μm membrane filter and aged at 37 ° C. for 60 minutes, 3.6 mg of calcium chloride dihydrate was added to 100 ml of this solution, stirred until dissolved, and left at 37 ° C. for 45 minutes. While the solution was stirred, 200 mg of lysozyme was slowly added and aged for 30 minutes, and then 100 mg of mucin was added slowly and left for 5 minutes. Finally, 17.47 mg of albumin was added to the solution to dissolve it, and the artificial lacrimal fluid was prepared by adjusting the pH to 7.2.

Experimental Example 1 Protein Removal Effect on Soft Contact Lens Group I

In order to examine the protein removal effect of the multi-purpose contact lens care solution according to the present invention, the following experiment was conducted using soft contact lens group I (EDGE ^R III, 35% moisture content, Ocular Sciences).

The new contact lens, which was never used, was immersed in 2 ml of the artificial lacrimal fluid of the preparation and shaken for 24 hours at 60 rpm in a stirred bath at 37 ° C. to deposit the protein on the lens. The lens was taken out and placed on the palm, and the drops of the multipurpose lens care solution of Examples 1 to 8 were dropped by 6 drops, followed by wiping the front and back surfaces of the lens 10 times using a middle finger. The lens was rinsed and then immersed in the multipurpose lens care solution and preserved for 8 hours.

After 8 hours, the lens was removed, and the amount of protein remaining in the lens was measured by the ninhydrin method, and the protein removal rate (%) was calculated by the following Equation 1 .

Meanwhile, as a comparative example, an existing commercial product (US Pat. No. 5,858,937) using phosphonate was used as a protein removing agent, and the results are shown in Table 2 below.

Protein removal rate for soft contact lens group I Lens care solution Amount of protein before washing (μg) Amount of protein after washing (μg) Protein removal rate ^a (%) Example 1 1.71 ± 0.20 0.66 ± 0.25 61.4 Example 2 1.75 ± 0.20 0.61 ± 0.15 65.1 Example 3 1.70 ± 0.21 0.69 ± 0.16 59.3 Example 4 1.76 ± 0.13 0.70 ± 0.10 60.5 Example 5 1.73 ± 0.15 0.71 ± 0.24 58.9 Example 6 1.75 ± 0.20 0.72 ± 0.10 58.7 Example 7 1.70 ± 0.21 0.64 ± 0.17 62.1 Example 8 1.76 ± 0.13 0.75 ± 0.22 57.5 Original commercial item 1.71 ± 0.20 1.39 ± 0.41 18.7 a: Average of 15 replicates

As shown in Table 2 , the protein removal rate of the multipurpose contact lens care solution according to the present invention was 58% to 65%, compared to the protein removal rate of the existing commercial product 18.7%, it was confirmed that the protein removal ability is much better.

Experimental Example 2 Protein Removal Effect on Soft Contact Lens Group IV

In order to examine the protein removal effect of the multipurpose contact lens care solution according to the present invention, experiments were conducted using soft contact lens group IV (Scustom ^™ , 55% moisture content, Specialty Ultravision Inc.).

The solution used in the experiment and the experimental method are the same as in Experimental Example 1, and as a comparative example, a commercial product containing phosphonate as a protein removing agent (US Pat. No. 5,858,937) and a subprotein degrading enzyme as a protein removing agent Proteolytic preparations containing subtilisin (Complete ^R , Allergan) were used. The experimental results are shown in Table 3 below.

Protein Removal Rate for Soft Contact Lenses Group IV Lens care solution Amount of protein before washing (μg) Amount of protein after washing (μg) Protein removal rate ^a (%) Example 1 1648.80 ± 268.16 1077.07 ± 34.68 34.68 Example 2 1648.80 ± 268.16 1217.33 ± 72.09 26.17 Example 3 1648.80 ± 268.16 1172.00 ± 140.81 28.92 Example 4 1648.80 ± 268.16 1097.33 ± 120.31 33.45 Example 5 1648.80 ± 268.16 1044.53 ± 99.27 36.65 Example 6 1648.80 ± 268.16 1112.28 ± 92.32 32.54 Example 7 1648.80 ± 268.16 1150.04 ± 111.36 30.25 Example 8 1648.80 ± 268.16 1111.62 ± 98.57 32.58 Original commercial item 1648.80 ± 268.16 1576.27 ± 198.01 4.40 Enzyme Preparation 1648.80 ± 268.16 1441.05 ± 120.38 12.60 a: mean of 15 replicates

As shown in Table 3 , the protein removal rate was only about 4% for the existing commercial products for the purpose of cleaning the contact lens, and the protein removal rate was only 12.6% for the commercially available enzyme preparations that are primarily intended for protein removal. . On the other hand, the multi-purpose contact lens care solution according to the present invention showed a protein removal rate of 30% or more and up to 36%, and it was confirmed that the protein removal effect was excellent even for the soft lens of group IV.

Experimental Example 3 Protein Removal Effect on RGP Contact Lenses

In order to examine the protein removal effect of the multipurpose contact lens care solution according to the present invention, the experiment was conducted using RGP contact lenses (RGP specialty, Specialty Ultravision Inc.).

The solution used in the experiment and the experimental method were the same as in Experimental Example 1, and as a comparative example, an existing commercial product containing phosphonate (phosphate) was used as a protein removing agent (US Pat. No. 5,858,937). The experimental results are shown in Table 4 below.

Protein Removal Rate for RGP Contact Lenses Lens care solution Amount of protein before washing (μg) Amount of protein after washing (μg) Protein removal rate ^a (%) Example 1 4.73 ± 2.05 2.99 ± 0.35 36.79 Example 2 4.73 ± 2.05 3.19 ± 0.56 32.65 Example 3 4.73 ± 2.05 3.14 ± 0.28 33.68 Example 4 4.73 ± 2.05 3.06 ± 0.69 35.24 Example 5 4.73 ± 2.05 3.14 ± 0.35 33.67 Example 6 4.73 ± 2.05 3.17 ± 0.78 32.98 Example 7 4.73 ± 2.05 3.02 ± 0.26 36.21 Example 8 4.73 ± 2.05 3.03 ± 0.33 35.98 Original commercial item 4.73 ± 2.05 3.32 ± 1.09 29.81 a: mean of 15 replicates

As can be seen in Table 4 , the conventional commercial product containing phosphonate as a protein remover was 29.8% protein removal rate for the RGP contact lens, the protein removal rate is more than 30% for the multi-purpose contact lens care solution according to the present invention In addition, up to 36.2%, the protein removal effect was improved by about 10% compared to the existing product.

Experimental Example 4 Disinfection and Preservation Effect

In order to determine the disinfecting and preserving power of the multi-purpose contact lens care solution according to the present invention, the following experiment was conducted using a 3M Petrifilm ^TM plate.

The top film of the Petri film plate was rolled up, and then approximately 1 ml of the prepared sample was inoculated vertically in the center of the lower film, covered with the top film, and gently pressed with a pressing plate to gel. The petri film for general bacteria was incubated for 48 hours in an incubator at 32 ° C., and the petri film for mold and yeast was incubated for 5 days in an incubator at 25 ° C. After culturing, the number of microorganisms was visually observed to measure disinfection and preservation of the multipurpose lens care solution. As a control, distilled water was used instead of the lens care solution according to the present invention. The experimental results are shown in Table 5 below.

Disinfection and Preservation Effect Lens care solution Common germ Mold, yeast Example 1 － － Example 2 － － Example 3 － － Example 4 － － Example 5 － － Example 6 － － Example 7 － － Example 8 － － Distilled water +++ +++ Without germs; -When there are fewer than 10 bacteria; + When less than 100 bacteria; When ++ bacteria are 100 or more; +++

As can be seen in Table 5 , the microorganisms were grown in general distilled water, but the microorganisms did not grow at all in the lens care solution according to the present invention. Therefore, it was found that the multi-purpose contact lens care solution according to the present invention has excellent effects of disinfecting and disinfecting microorganisms present in the lens and preserving the lens in a disinfected state for a relatively long time.

Experimental Example 5 Eye Mud Stimulation Test

In order to examine the safety of the multi-purpose contact lens care solution according to the present invention, the following eye mucosal stimulation experiments were conducted based on the guidelines announced by the National Institute of Health and Safety.

Before the test, the eyes were examined with the naked eye and an ophthalmoscope, and only 9 rabbits (New Zealand White, 2.5 ± 0.5 kg) were selected for each group, and 100 μl of each test substance (contact lens care solution) was applied to the left eye. It was. Six of the nine were left as it was, and the other three were washed with physiological saline for about one minute after 20 to 30 seconds. The left eye was observed 1, 2, 3, 4, 6, 24, 48, and 72 hours after the eye drop by visual observation, flashlight observation, and ophthalmoscope observation, using the right eye without the test substance as a control. The results are shown in Table 6 below.

Eye mucosal stimulation test results Lens Care Solution Elapsed time (hours) One 2 3 4 6 24 48 72 Example 1 T.S. 0 0 0 0 0 0 0 0 M.O.I. 0 0 0 0 0 0 0 0 Example 2 T.S. 0 0 0 0 0 0 0 0 M.O.I. 0 0 0 0 0 0 0 0 Example 3 T.S. 0 0 0 0 0 0 0 0 M.O.I. 0 0 0 0 0 0 0 0 Example 4 T.S. 0 0 0 0 0 0 0 0 M.O.I. 0 0 0 0 0 0 0 0 Example 5 T.S. 0 0 0 0 0 0 0 0 M.O.I. 0 0 0 0 0 0 0 0 Example 6 T.S. 0 0 0 0 0 0 0 0 M.O.I. 0 0 0 0 0 0 0 0 Example 7 T.S. 0 0 0 0 0 0 0 0 M.O.I. 0 0 0 0 0 0 0 0 Example 8 T.S. 0 0 0 0 0 0 0 0 M.O.I. 0 0 0 0 0 0 0 0 T.S. : Total mucosal irritation index M.O.I. : Mean Eye Mud Stimulation Index

As can be seen in Table 6 , the multi-purpose contact lens care solution according to the present invention did not irritate the eye mucosa even after 72 hours after instillation, it was confirmed that the safety is excellent.

As described above, the multi-purpose contact lens care solution composition according to the present invention effectively removes protein components deposited on the lens from the lacrimal component without causing discoloration or deformation of the lens by the components of the composition and for a long time with respect to the lens. It exhibits a continuous disinfection and preservation effect, which can prolong the wearing time and life of the lens. In addition, the composition does not contain eye irritants such as proteolytic enzymes, phosphonates, hydrogen peroxide, and does not contain irritating low molecular weight additives such as low molecular weight preservatives, disinfectants, surfactants, etc. It is safe to use and prevents eye diseases caused by lens and lens care solution. Furthermore, the multi-purpose contact lens care solution composition according to the present invention exhibits excellent effects on all types of contact lenses, such as hard contact lenses and RGP contact lenses, which are not only soft contact lenses but also have low moisture content due to the characteristics of the material, which are easy to burden the eyes. Cleaning, rinsing, disinfection, preservation and protein removal can be performed at once without the need for any device or device, thereby reducing the cost of lens management.

Claims (12)

0.005 to 10% by weight of a polysaccharide having a molecular weight of 5,000 or more as a non-enzyme protein removing agent; 0.01-10% by weight of nonionic surfactant; And 0.00001 to 1% by weight of a polymeric preservative. The contact lens care solution composition of claim 1 comprising a stabilizer or a buffer solution in addition to said component. The contact lens care solution composition of claim 1, wherein the polysaccharide having a molecular weight of 5,000 or more is selected from the group consisting of hyaluronic acid, sodium hyaluronate, and chondroitin sulfate. The method of claim 1, wherein the nonionic surfactant is selected from the group consisting of polyethylene oxide / polypropylene oxide copolymer and tetra (polyoxyethylene / polyoxypropylene) ethylenediamine block copolymer having a molecular weight of 5,000 or more. Contact Lens Care Solution Composition. The method of claim 4, wherein the polyethylene oxide / polypropylene oxide copolymer has an average molecular weight of 8,350 (ethylene oxide content: 82.0 wt%; poloxamer 188), 12,000 (ethylene oxide content: 73.0 wt%; poloxamer 338) and 15,000 (Ethylene oxide content: 83.0% by weight; poloxamer 407) a contact lens care solution composition, characterized in that it is selected from the group containing. The method of claim 4, wherein the tetra (polyoxyethylene / polyoxypropylene) ethylenediamine block copolymer has an average molecular weight of 6,700 (ethylene oxide content: 60.0 wt%; Tetronic 904), 15,000 (ethylene oxide content: 70.0 wt% A contact lens care solution composition selected from the group consisting of Tetronic 1107) and 25,000 (ethylene oxide content: 60.0 wt%; Tetronic 908). The method of claim 1, wherein the polymeric preservative is hydrolyzed lauryldimonium hydroxypropyl collagen, hydrolyzed cocodimonium hydroxypropyl collagen, hydrolyzed stearyldimonium hydroxypropyl collagen, poly [oxyethylene (dimethyl is Mino) ethylene (dimethylimino) ethylene dichloride], polyaminopropyldiguanide and polyquaternium-16, the contact lens care solution composition. The contact lens care solution of claim 2, wherein the stabilizer is selected from the group consisting of ethylenediamine tetraacetic dihydrogen acetate, ethylenediamine tetrasodium triacetate and ethylenediamine tetrasodium tetrasodium tetrasodium Composition. The contact lens care solution composition of claim 2 comprising 0.01 to 0.5% by weight of said stabilizer. 3. The contact lens care solution composition of claim 2 wherein the buffer solution is selected from the group comprising borate buffer, phosphate buffer and carbonate buffer solution. The contact lens care solution composition of claim 2 wherein the buffer solution has a pH of 6.0-8.0. 2. The contact lens care solution composition of claim 1 wherein the contact lens care solution is for cleaning, disinfecting, preserving and removing proteins of the soft contact lens, the hard contact lens and the RGP contact lens.