KR100352643B1 - natural antiseptic substance made from mixture of natural mugwort and silver - Google Patents

Abstract

The present invention relates to a natural preservative prepared by mixing natural mugwort and metallic silver (Ag), wherein the natural mugwort is an extraction solution by chemical treatment, and the silver (Ag) is made of a colloidal solution in an ionic state. . The natural mugwort extract is obtained by dissolving ethyl acetate in the filtrate after dissolving in ethyl acetate solution to obtain an intermediate mugwort extract, and mixing and stirring the activated mugwort with activated carbon after dissolving ethanol, using the supernatant produced by centrifugation. Mugwort extract is made of more than 3% concentration, the concentration of the silver ion colloid is preferably made to be more than 10ppm. Therefore, the present invention has an effect of providing a new natural preservative having better antiseptic ability without exhibiting any side effects of the conventional chemical synthetic preservatives to the human body.

Description

Natural antiseptic substance made from mixture of natural mugwort and silver}

The present invention relates to a natural preservative, and more particularly to a natural preservative prepared by mixing natural mugwort and metal silver (Ag).

In general, food and cosmetics widely used around our lives are added to the human body to prevent the change of internal properties and to preserve for a certain period of time to use a preservative. The preservative effectively inhibits the action of foreign substances such as bacteria and fungi to prevent the degeneration of food and cosmetics, etc., and preservatives having such antiseptic function, there are some substances produced naturally, but usually for convenience of manufacture or Artificial materials synthesized chemically will be used for mass production.

For example, currently used cosmetics are made up of a mixture of various substances based on polymeric lipids or water. Among cosmetic substances, glycerin or sorbitol, which is a carbon source of microorganisms, is contained, and as a nitrogen source, many amino acid derivatives and proteins are incorporated. Therefore, bacterial fungi, which are mediated by the carbon source and the nitrogen source, are multiplied and easily polluted. On the other hand, the cosmetics, compared with the food in general, because the use period is extremely long, there are more problems in the preservative treatment than in food. Basic conditions for preservatives to be considered by the characteristics of these cosmetics should be antimicrobial effect on all microorganisms, easy to use and manufacture, and no skin irritation. In addition, to be usable universally in addition to the above conditions, it should be easy to handle without being affected by the pH of the product, without degrading the effect of the product components.

In order to satisfy these conditions, preservatives currently used in general cosmetics, paraoxybenzoic acid esters and parabens are mainly used, and for the purpose of sterilization, benzalkonium chloride, chlorohexidine glyconate and trichlorocarbanil This is used. Currently, the above-mentioned materials used in existing products are manufactured through chemical synthesis to perform functions as preservatives, but these chemicals cause skin irritation and cause skin edema or allergy symptoms. There is a problem that hurts.

Therefore, in order to solve the side effects of the conventional chemical synthetic preservatives, the researches on natural preservatives that are made naturally and are made in nature have been actively conducted in recent years. The inventors of the present invention have been able to find out that natural mugwort and silver (Ag) metals widely used in folklore have antiseptic properties while studying the superiority of such natural preservatives. It was found that the ionic colloid mixture of the extract and the metal silver (Ag) was excellent in preservative ability, and developed a natural preservative by this manufacturing method.

Accordingly, an object of the present invention is to provide a new natural preservative having better antiseptic ability without exhibiting any side effects of the conventional chemical synthetic preservative.

Figure 1-Figure showing the manufacturing process of natural preservatives according to the present invention.

Figure 2 is a schematic of a device for producing silver colloids of the natural preservatives according to the present invention.

Figure 3-Figure showing the antimicrobial activity of natural mugwort as a component of natural preservatives according to the present invention.

Figure 4 is a component of the natural preservative according to the present invention showing the antimicrobial activity of silver colloid.

Figure 5-The composition of the natural preservatives according to the present invention is showing the various mixing ratios of natural mugwort and silver colloid.

Figure 6 is a diagram showing the rise of the antimicrobial activity of the natural preservative according to the present invention.

The natural preservative according to the present invention for achieving the above object is to provide a natural preservative using natural mugwort and silver (Ag) consisting of a mixture of natural mugwort and metal silver (Ag). Preferably, the natural mugwort is an extraction solution by chemical treatment, and the silver (Ag) is made of a colloidal solution in an ionic state.

The natural mugwort extract is obtained by dissolving ethyl acetate in the filtrate after dissolving in ethyl acetate solution to obtain an intermediate mugwort extract, and mixing and stirring the activated mugwort with activated carbon after dissolving ethanol, using the supernatant produced by centrifugation. Mugwort extract is made of more than 3% concentration, the concentration of the silver ion colloid is preferably made to be more than 10ppm.

Next, the features and applications of the natural preservative according to the present invention having the above configuration will be apparent through the following description, and the natural preservative and its manufacturing method will be described in detail with reference to the accompanying drawings.

Basically, the present invention was prepared by obtaining a silver (Ag) colloid by electrolysis from natural mugwort extract and metal silver (Ag) in order to perform the function as a natural preservative. First, it will be described with reference to Figure 1 showing the process of extracting a substance having antiseptic properties from natural mugwort.

Natural mugwort used as the material of the present invention was obtained from Ganghwado, and used to dry the mugwort. First, the dry reinforced mugwort is crushed by a grinder to crush the particles. (I) In the present invention, since the extract of natural mugwort is used as a raw material in the present invention, it is crushed to facilitate the extraction of raw materials. Since the pulverization may be performed using a general pulverizer, detailed description thereof will be omitted.

Next, the active ingredient of the ground natural mugwort is extracted. (II) That is, the dried natural mugwort is soaked in ethyl acetate and left for 1 to 2 days. Natural mugwort contained in the ethyl acetate solution is the active ingredient is dissolved in the ethyl acetate solution over time.

The ethyl acetate of the filtrate is evaporated to obtain a mugwort extract. (III) That is, the ethyl acetate solution is filtered through a 0.45 µm filter paper, and the filtrate is evaporated under reduced pressure using a pressure reducer to evaporate ethyl acetate to obtain an intermediate extract. At this time, when the ethyl acetate is completely evaporated, the middle mugwort extract in a gel state remains.

Next, the gel-like extract is dissolved by adding 50% by weight of ethanol to ethyl acetate. (IV) Then, the portion which is not dissolved in ethanol is filtered out using a 0.45 μm filter paper and the filtrate is collected. (V) 50% by weight of the extract is mixed with activated carbon and stirred for one day, followed by centrifugation for about 10 minutes at a speed of about 1200rpm. A fraction is produced, and this clear supernatant is fractionated to obtain wormwood extract according to the present invention.

Next, a process of generating silver (Ag) ions according to the present invention will be described with reference to the drawing of FIG. 2. First, it is known that metal silver (Ag) has some antibacterial property per se. However, since the cosmetics used in the present invention are applied to the face, hands, and the like of the human body, the metal silver (Ag) may be mixed, but in this case, the metal silver (Ag) may be exposed to the skin, resulting in aesthetic difficulties. In other words, even if fine silver (Ag) powder is used, it is difficult to mix with other ingredients in the cosmetic, and thus, when the aggregated silver is applied to the skin, it is not preferable to mix it with the cosmetic as it is. Therefore, the present inventors found that silver (Ag) ion state was more effective for antibacterial than silver (Ag) metal itself, and it was also found that even mixing with other components in cosmetics was possible. Hereinafter, a manufacturing process of such silver (Ag) ions will be described.

As shown in FIG. 2, the inventors simply implemented a device to prepare silver (Ag) ions in a colloidal state. That is, a predetermined amount of distilled water was contained in the main body 10, and a rod 14 of pure metallic silver (Ag) having a purity of 99.99% was installed at the anode of the power source. The surface 12 of the body was browned to block external ultraviolet rays. Then, the power source 16 was connected to the main body cover 17, and silver was electrolyzed by applying a constant current and voltage from the outside. That is, as the power switch 18 is operated, a certain amount of current and voltage (a voltage of about 30 volts is preferably applied) is applied to pure silver (more than 99.99% of pure silver), thereby releasing silver ions (Ag ²⁺ ). The silver ions (Ag ²⁺ ) are colloidalized by distillation in distilled water, and the operation of the power source can be confirmed by the power lamp 15. In addition, an improved method may be prepared by accelerating the release rate of silver ions by electrolyzing pure distilled water. Put and connect the electrodes. The silver solution prepared as described above was confirmed by the inductively coupled plasma emission spectrometer that silver ions (Ag ²⁺ ) were present in the colloidal state, and the concentration thereof was also measured. The natural preservative required by the present invention is prepared by simply physically mixing the mugwort extract solution and the colloidal silver ion solution. That is, by mixing the mugwort extract solution and the silver ion solution, each individual silver ion is uniformly dispersed in the solution of the mugwort extract. The mixing ratio of the mugwort extract solution and the silver ion solution may be various, but it is to be mixed at the minimum mixing ratio as shown in the following experiment to implement the function as a preservative required in the present invention. In other words, in order to realize the function as a natural preservative required in the present invention, as the mugwort extract and the silver ion solution are mixed, the synergistic effect is increased. It is preferable to mix.

Next, using the natural mugwort extract and silver ion colloid prepared as described above was carried out experiment of the preservative through the following examples.

Before describing each embodiment, various experimental items used in the present invention will be described. Antimicrobial activity was measured by escherichia coli, Bacillus subtilis and Staphylococcus aureus at 37 ° C in LB medium. It was preincubated for about 18 hours. Pseudomonas aeruginosa and Candida albicans were preincubated for about 18 hours at a temperature of about 30 ° C. in LB medium. Thereafter, the antimicrobial activity was examined by culturing the whole culture solution of the above-described strains for 24 hours after dispensing in the silver solution, wormwood extract prepared by the present invention and the mixed solution of silver solution and wormwood extract. Hereinafter, the natural preservatives according to the present invention according to the mixing of the silver solution and wormwood extract according to the present invention in each embodiment will be described in detail, but it can be seen that the concept according to the present invention is not limited to the following examples. .

Example 1 Antibacterial Effect of Fortified Wormwood

The medium was made with the strengthened wormwood extracted by the method of FIG. 1 described above. The culture medium was prepared by adding mugwort extract to 1.6%, 3.3%, 5% and 6.6%, respectively, and inoculated with the precultured strains, and incubated for 24 hours. The result is shown in FIG. As shown, Esccherichia coli, Bacillus subtilis and Staphylococcus aureus were all killed at 1.6%, the lowest concentration of fortified wormwood extract according to the present invention. However, Pseudomonas aeruginosa and Candida albicans are not completely killed at 1.6% but are completely killed at 3.3%. Therefore, it can be seen that the concentration of 3.3% or more is suitable as the wormwood extract of the present invention alone.

Example 2 Antimicrobial Effect of Silver Colloid

The silver colloid prepared by the method of FIG. 2 described above was inoculated with each strain preincubated in a medium made to have a concentration of 10ppm, 20ppm, 30ppm, 40ppm and 50ppm and incubated for 24 hours. Then, the absorbance was measured at 600 nanometers to determine the antimicrobial activity, and the results are shown in FIG. 4. Figure 4 shows the experimental results, it was found that the more the amount of the silver colloid is more effective, simply examined the minimum amount of silver colloid. As shown, each of the strains described above shows a clear difference in the medium containing the silver colloid and not the medium, it can be seen that the medium provided as a control is activated by increasing the population gradually over time. However, Candida albicans showed a significant inhibition of the antimicrobial activity even under 10ppm, the antimicrobial activity was almost inhibited at a concentration of about 20ppm, the remaining four strains were found to inhibit the antimicrobial activity even under 10ppm.

Example 3 Determination of the Mixing Ratio of Natural Mugwort Extract and Silver Colloid

As described above, each of the natural mugwort extract and the silver colloidal solution exhibited antimicrobial activity, but the two mixtures showed more excellent antimicrobial activity, and the mixing ratio for optimum inhibition of the antimicrobial activity was tested. . Inoculate each strain precultured with silver colloid 10ppm in different concentrations of natural mugwort extract and incubate for 24 hours. Thereafter, the number of viable cells was examined to determine the antimicrobial activity to determine the optimum mixing ratio. The results are shown in Figure 5, as shown, it can be seen that the concentration of about 3.5% of the natural mugwort is optimal. Although not shown, it can be seen that as the concentration of natural mugwort gradually increases, the antimicrobial activity is inhibited. In the present experiment, the silver colloid concentration was represented as 10 ppm, but as the concentration of the silver colloid also increased, it was obvious that the antimicrobial activity was further suppressed.

Example 4 Comparison of Antimicrobial Activity of Silver Colloid, Natural Mugwort Extract and Mixed Solution of Silver Colloid and Natural Mugwort Extract

The antimicrobial activity of silver colloid, antibacterial activity of natural mugwort extract and the antimicrobial activity of these mixed solutions according to Examples 1, 2 and 3 were compared, respectively, and the results were shown in FIG. For convenience of explanation, a mixed solution of silver colloid and natural mugwort extract will be referred to as Dong-eui Amaranth Silver Artemisia (DASA). As shown, it can be seen that DASA mixed with them is more effective than the use of silver colloid and natural mugwort extract alone.

Example 5 Stability

The stability of silver colloids, natural mugwort extracts and their mixed solution for DASA for each of Examples 1, 2 and 3 described above was measured for 4 weeks at weekly intervals. Five precultured strains were plated on a solid plate medium and silver colloid and natural mugwort were dispensed on it to measure the area of the antimicrobial zone. Table 1 shows the antimicrobial activity stability of silver colloid, Table 2 shows the antimicrobial activity stability of natural mugwort.

Table 1

TABLE 2

As shown in the data, for one month, silver colloid and natural mugwort extract do not show a significant difference in antimicrobial activity, which can be seen as stable. And although not shown, DASA also shows the same stability.

Example 6 Side Effects Test

Eleven women in their 20s to 40s were tested for side effects. Changes were observed 48 hours after the application of silver colloid, natural mugwort extract, DASA and DASA added to the arm near the armpit. None of them could observe special side effects or swelling, and these experimental results show that they are more effective than the chemically synthesized preservatives contained in conventional cosmetics.

Example 7 Comparison of Preservative Effects of Preservatives and DASA in Products

The preservative effect was compared by removing preservatives, adding preservatives, and adding DASA. For the experiment, cosmetics used skins and creams widely used by consumers. Staphylococcus aureus was cultured and dispensed into skin and cream at 6.72 × 10 ⁷ / g to compare the preservative effect by measuring the viable cell count for 4 weeks. The results in the skin are shown in Table 3 and the results in the cream in Table 4.

TABLE 3

Table 4

As shown in the table, when the preservatives are removed from the product, the number of bacteria decreases initially and then gradually increases, that is, the degeneration of cosmetics due to the propagation of microorganisms appears, but the existing synthetic preservatives and DASA do not have time. As the number of bacteria passed, the live bacteria could not be found after 4 weeks. That is, it indicates that the preservative ability of the existing synthetic preservatives and DASA is almost no difference, it can be seen that DASA is suitable as a preservative.

As described above, according to the present invention, while having the same antiseptic effect as the existing preservatives, no side effects occur in the human body such as skin swelling and tickling, so it is safe for the general public as well as specific constitution for allergy. It is effective in providing natural preservatives that can be used.

Claims (4)

After dissolving in ethyl acetate solution, the ethyl acetate of the filtrate is evaporated to obtain intermediate mugwort extract, and the extract is mixed with agitation with activated carbon after dissolving ethanol, followed by separating the supernatant produced by centrifugation. and; A natural preservative using a mixture of natural mugwort and silver (Ag), characterized by physically mixing a colloidal solution containing silver ions (Ag ²⁺ ) of 10ppm or more. delete delete delete