KR100337522B1 - Treatment of grass gene abstraction - Google Patents

Abstract

The present invention develops and extracts ovarian grass seed Zoysia grass at low temperature to incubate at 25 ° C. to prevent low temperature disorders and verbs and to create resistance.

Description

Grass seed genetic factor extraction method {Treatment of grass gene abstraction}

The present invention relates to a grass seed genetic factor extraction treatment method is a grass seed Zoysia grass native to all areas of our country. On the other hand, due to the increase in grass demand and the decrease of natural grass, the cultivated grass is grown a lot these days. When seeds are used for grass seed propagation, grass seeds play an extremely important role. Genetic purity is important, and good grass seed characteristics and minimum temperature after germination of grass seed are used for extraction.

Cold disorders and verbs

In the case of egg-shaped grass, menstrual abnormalities occur at a low temperature of about 15 ° C, menstrual metabolism stops below 10 ° C, and if the temperature drops below 0 ° C, the company is expected. However, low temperature disturbances differ depending on the degree of resistance and the growing environment. It also depends on the state of grass seed development.

The most important material requirement for growth and maintenance for turf cell culture is temperature, which must be kept constant. If the number of kelus cultures to be handled is small, a standard incubator for cultivating microorganisms that can be maintained at 25 ± 2 ° C is very suitable and a constant temperature room of 25 ° C is required to obtain a higher amount of callus.

Grass seed temperature at 30 ℃ for 7 days

7 days at 25 ° C 7 days after development

7 days at 15 ° C 7 days at 10 ° C 7 days at 5 ° C

Vacuum at -5 ℃ for 7 days at 0 ℃

¹⁰ to thereby the 7 days of the culturing to ² mmHg. Treatment method to cultivate the grown seeds extracted at low temperature by treatment to 25 ℃

The perspective view of grass seed

Fig. 2 Section of Grass Seed

Third Section of Grass Seed

Example 1

Grass seed development

By treating as shown in Table 1, five developmental conditions were extracted at the germination temperature of 0 ° C.

Example 2

Developmental state

Hayeoteum treated with ² mmHg ^- 10 as described above.

Claims (1)

Nanjihyeong vacuum 10 at a low temperature below -5 ℃ as Zoysia grass ^- by extraction with ² mmHg processing method of the culture