KR0148462B1 - Preparation process of brainactivating soft capsule - Google Patents

Abstract

Recently, the problems of brain activation, such as improving the learning effect of the examinee and prevention of Alzheimer-type dementia, have emerged seriously.

Therefore, in addition to suppressing LDL-cholesterol in the blood and reducing arteriosclerosis index, DHA and soy lecithin, which are used as main ingredients of the present invention, are the main components of the brain. And Aloe vera's animal experiments have been shown to be effective in activating the brain, thus promoting the synergistic effects of these Aloe and D. It relates to a method for producing a soft activator soft capsule of the present invention to activate the.

The main ingredient of the present invention is to mix and homogenize aloe vera freeze-dried powder and high purity DHA-containing fish oil extracted and purified from blue fish.

Next, soybean lecithin is added as a subsidiary ingredient, followed by tocopherol as a natural antioxidant, ascorbic acid as a nutrition enhancer, and tyrosine, which is supplemented with glucose used only as brain energy and acetylcholine, a neuron in the brain. It is prepared by mixing, homogenizing, mixing and homogenizing cooking oil and wax according to the mixing ratio. It is a manufacturing method of the brain active agent soft capsule, which is used as an improvement in learning ability and prevention and improvement of senile dementia.

Description

Preparation method of brain activator soft capsule

The present invention is a brain supplement component as one of the main fatty acid components of aquatic foods in powder products obtained by freezing and drying the effective pharmacological ingredients of Aloe vera, which are known to be excellent in preventing and improving intractable diseases as natural herbal medicines and health foods. The main raw material is known as docosahexaenoic acid (DHA), and tocopherol and ascorbic acid are added and mixed as lecithin and natural antioxidant, which are known as brain aids in plant components.

In addition, tyrosine, which supplements glucose and acetylcholine, which are brain energy sources, is added to it, and oil-activated soft capsules of brain activator as a health supplement for brain activation using cooking oil and wax as a molding agent. It relates to a manufacturing method.

With the recent economic growth, environmental damage, water pollution, and flooding of instant foods caused by industrialization and industrialization have severely threatened our health.

In particular, the excessive intake of nutrition due to the increase of our income and the absolute lack of exercise due to the development of mechanical civilization not only promote obesity, but also contribute to arteriosclerosis, hypertension, coronary heart disease, Chronic degenerative adult diseases such as myocardial infarction, angina pectoris and stroke are prevalent. In addition, elderly Alzheimer-type dementia patients are increasing year by year. In addition, too many young students are taking seriously the serious development of the brain due to excessive study.

Therefore, the purpose of the invention is to invent a soft capsule as an active agent of the brain that can prevent and improve dementia of the elderly by improving learning efficiency and promoting brain activity.

Aloe vera has received much attention as a health medicine because of its excellent preventive and therapeutic effects such as digestive ulcers, intractable dermatitis, circulatory disorders, rheumatoid arthritis, liver disease, kidney and urinary inflammation, respiratory diseases, diabetes, and anemia. However, many clinical studies are being discovered one after another.

On the other hand, DHA, which is contained in a large amount of blue fish, has recently become a global topic because of its good hair, and many studies have been conducted. Dr. Suzuki of Japan, in his dissertation aging and DHA in the DHA, through the maze test of memory learning ability, DH has the ability to improve learning efficiency and control brain function, and also Alzheimer-type Reported effective in preventing dementia. The author's research also confirmed that the major fatty acid in the brain is DHA.

Therefore, in the present invention, the main ingredients are lyophilized powder of Aloe vera, which has been scientifically proven, and DHA in fish oil, and lecithin and natural separated from legumes. Tocopherol, ascorbic acid, and the like were added as an antioxidant.

Again, the mixture was homogenized by adding tyrosine as an amino acid component that supplements the function of glucose and acetylcholine, which are neurotransmitters of the brain, to be used for nutrition of the brain. Brain activator soft capsule was invented using cooking oil, wax, etc. as a molding agent of soft capsule.

The storage test for the product stability of soft capsules as a brain activator is based on the stability test method used to test the stability of medicines. The characteristics of the product for 3 months at 40 ° C (± 1 ° C) and 75% RH (± 5% RH) And component changes and sensory tests.

The material of the animal test, the test method, and the test result of the present invention are as follows.

I. Experimental Materials

1. Animal Experiment

The experimental animals used for this experiment were Sprague Dawley (SD) male rats purchased from the Korea Research Institute of Chemistry and pre- bred for 2 weeks, and then rats weighing 90 ± 10 g were weighed 10 rats per group for 7 weeks. Animal experiments were conducted.

At this time, the conditions of breeding in the animal room were the content of cholesterol and low density lipoprotein (LDL) -cholesterol in the blood in the animal breeding room where temperature (22 ± 2 ℃) and humidity (65 ± 3% RH) are automatically controlled. The synergistic effects of Aloe and DHA on atherosclerosis were evaluated.

And as an indirect assessment of brain activity, the synergistic effects of Aloe and DHA on the functional damage of neuronal cells and the activity of free radical scavenging enzymes caused by lipid peroxide produced by free radicals, a free radical species, Compare and evaluate.

2. Reagent

The aloe vera product used in the development of the present invention uses a freeze-dried powder devoted by Kim Jung Moon Aloe Co., Ltd., and lipid peroxide produced by free radicals as reactive oxygen species (ROS) in the blood. The reagent to be used for the production and the enzyme to be used for the determination of the activity of superoxide dismutase (SOD) as free radical scavenging enzyme were used as the sigma-express reagent, and all other related reagents were used as the special reagent.

II. Experiment method

1. Preparation of experimental feed

Animal feed used in this experiment was 44.0% of corn starch and 13.3% of carbohydrate, 57.3% of total carbohydrate, 18.0% of casein for protein, and 16.0% of lard for lipid. In addition, cellulose (3.0%), minerals and vitamins were added to 1.0% and 3.5% of AIN's animal test products, respectively, DL-methionine 0.3% and choline chloride 0.2% It was added and prepared and used.

In addition, hypercholesterolemia was induced by adding 0.5% cholesterol and 0.2% sodium cholate (Na-cholate) to the experimental feed to investigate the inhibitory effect of cholesterol. In addition, the 1.0% aloe group reduced the amount of constant in carbohydrates by 1.0%, and the 3.0% group of DHA reduced 3.0% of the lipid in pig fat (lard). The Aloe + DHA) group prepared a diet by reducing 1.0% of aloe and 3.0% of aH.

2. Animal testing for effect test

Free-feeding the prepared foods of the control group which added nothing, the aloe 1.0% group, the DHA 3.0% group, and the aloe and DH mixed (1.0% + 3.0%) group as a basis weight daily Of course it was taken freely.

Daily feed intake was calculated by measuring the weight of the formula and the amount of the rations administered at 5:30 pm every day and the basis weight.

3. Measurement of cholesterol content

The total cholesterol content was measured by the ortho-phthaladehyde method according to the method of Rudel et al. (1973) and the total cholesterol content in serum was measured by a standard calibration curve. And the content of low density lipoprotein (LDL) -cholesterol was measured according to the method of Noma (Noma et al. (1978)) as follows.

① Measurement of HDL + LDL-cholesterol: 100 μl of serum is added to the test tube and 4.0 ml of sodium heparin / sodium chloride (150 mg + 4 g / 1000 ml DW) is added to the mixture and left to stand at room temperature for 15 minutes. Then, add 0.5 g of Amberlite IRA-400 to the test tube, mix and leave for 10 minutes.

After 10 minutes, centrifuge at 700xg for 10 minutes, transfer 2 ml of the supernatant to a new test tube, add 2.0 ml of 2.0 M fish (PCA) solution, mix, and then centrifuge at 700xg for 15 minutes.

② Measurement of HDL-cholesterol: 100 μl of blood serum is placed in a test tube, sodium heparin / calcium chloride / nickel chloride reagent (sodium heparin / chlcium chloride / sodium heparin / calcium chloride / nickel chloride: 300mg + 60mM + 2.0mM / 1000μlDW) ) 4.0 µl is added, mixed and allowed to stand at room temperature for 30 minutes, followed by centrifugation at 700xg for 15 minutes.

Transfer 2.0 ml of the supernatant obtained at this time to a new test tube, add 2.0 ml of 2.0 M PCA solution to each test tube, and mix and centrifuge again at 700xg for 15 minutes.

③ Determination of LDL-cholesterol: Centrifuge the two pretreated in the above ①②, discard the supernatant and color the reagent (50g of sulfosalicylic acid / 1000mL of acetic acid / acetic acid anhydride / C-sulfuric acid = 35 /). 65/10) 2.0 ml each was added and mixed, and the mixture was left at room temperature for 10 minutes, and then absorbance was measured at 625 nm of the spectrophotometer. The LDL-cholesterol was quantified by quantification using a standard calibration curve.

4. Calculation of arteriosclerosis index

The atherosclerosis index (AI), which is widely used as an index of atherosclerosis, known as an early symptom of adult disease, was obtained by subtracting HDL-cholesterol from total cholesterol in accordance with the method of Haglund et al. (1991). Again, it was calculated by dividing by HDL-cholesterol.

5. Determination of lipid peroxide content and SOD activity

The content of malondialdehyde (MDA) produced in serum was measured at 532 nm with a fluorophotometer according to the method of Yagi (1987), and the amount of malondialdehyde (MDA) was measured by a standard calibration curve.

In addition, it is widely used as an important indicator of physiological impairment of the organs including the brain, the development of geriatric disease, damage to cellular activity, and bioaging, and the content of lipid peroxide was measured according to the inventor's method using serum. Measured with spectrophotometer at 532 nm using 20% acetic acid, 8.1% SDS and 1.2% TV (TBA: pH3.5).

In addition, the measurement of superoxide dismurase (SOD) activity, which is the most important among the reactive oxygen species removal enzymes, was quantified according to the method of Oyanagui (1984).

The serum was diluted 100-fold as potassium phosphate buffer, and 100 μl of this was placed in a test tube, where 500 μl of distilled water, 200 μl of reagent A (3 mM hydroxylamine / 3 mM hypoxanthine) and reagent B (7.5 mU / ml xanthine oxidase with 0.1 mM). 200 ml of EDTA-2Na) was added, mixed well in a vortex, and then left in a water bath (37 ° C.) for 40 minutes.

To this reaction solution was added reaction solution, 2.0 ml of Reagent C (300 mg of sulfanilic acid / 5.0 mg of N-1-naphthyle-thylenediamine in 500 ml of 16.7% acetic acid), and the mixture was mixed well and allowed to stand at room temperature for 20 minutes. Next, the absorbance was measured at 550 nm of the spectrophotometer, and the superoxide dismutase (SOD) activity in serum was measured according to a standard calibration curve.

III.Experimental Results

First, the prevention and improvement effect of the adult disease is described as follows.

Table 1 shows a comparison of the total cholesterol low density lipoprotein (LDL) -cholesterol content in the blood, which is known to be a cause of adult disease, and the atherosclerotic index used as an index of development during atherosclerosis.

Compared with the total cholesterol content in serum, 82.5% added Aloe 1.0% and 71.9% added DH 3.0% compared to the control group (100%), while aloe and DC mixed (1.0% + 3.0%). ), The addition group was 69.5%, a significant synergistic effect was recognized.

In particular, comparing the content of LDL-cholesterol in the blood, which is widely used as an indicator of the development of adult diseases, compared to the control group (100%), aloe 1.0% added group was 83.0% and DH 3.0% added group was 66.8%. A significant synergistic effect was recognized as 60.5% in the group containing WHA mixed (1.0% + 3.0%).

In addition, when comparing the atherosclerosis index used as an indicator of atherosclerosis, the mixture of aloe and DH was mixed compared to the control group (100%), compared with 70.3%, 3.0%, and 65.9%, respectively. The addition group (1.0% + 3.0%) was 63.4%, showing a slight synergistic effect.

In conclusion, it can be seen that a combination of Aloe and DH is more effective in preventing and improving adult diseases than Aloe vera or DHA alone.

Second, the nerve cell damage and defense enzyme activity is described as follows.

Malondialdehyde (MDA) as a lipid peroxide in serum known to be produced by strong free radicals, which is known to be directly involved in the functional damage of tissue cells of each organ including brain nerve cells. Table 2 shows a comparison of the content of and the activity of the most powerful superoxide dismutase (SOD) among the free radical scavenging enzymes.

Production of malondialdehyde (MDA) as a lipid peroxide produced by strong free radicals predicted to affect functional damage of cells such as neural tissues, and superoxide dismutase as an enzyme for removing these free radicals. The effects of aloe (1.0%) and DH (3.0%), and mixtures (1.0 + 3.0%) on the activity of superoxide dismutase (SOA) are shown in Table 2.

The effect on the production of malondialdehyde was 93.3% for Aloe 1.0% and 89.4% for DM 3.0%, compared to the control group (100%) without Aloe or DC. It was found that the addition of HA (1.0% + 3.0%) significantly inhibited the production of lipid peroxide (MDA) by 84.4%.

In addition, the activity of superoxide dismutase (SOD) as a free radical scavenger was 105.2% in the group added with 1.0% aloe and 114.6% in the group added 3.0% compared to the control group (100%), while Aloe and D. The group of H (1.0% + 3.0%) added was 121.9%, which showed synergistically increasing the activity of superoxide dismutase (SOD) synergistically compared to the administration of Aloe or DHA alone.

Therefore, the administration of Aloha as a mixture of Aloe and DH (1.0% + 3.05) results in malondialdehyde (MDA) produced by free radicals that cause neuronal damage more effectively than Aloe or DH alone. In addition to effectively inhibiting the production of superoxide dismutase (SOD), the protective enzyme of these toxic free radicals effectively increases.

In summary, the soft carcass of the present invention made by mixing Aloe vera and DHA in 1: 3 effectively suppresses total cholesterol and low density lipoprotein (LDL) -cholesterol content. By effectively reducing the arteriosclerosis index, the prevention and improvement of adult disease will be evident.

In addition, it is effective in promoting blood flow to tissue cells such as the brain and inhibiting the production of cytotoxic free radicals, and effectively activating the activity of the free radical scavenger, a cytotoxic substance.

Third, the stability test of the brain activator prototype is performed as follows.

A. Preparation of Brain Activator Prototype

As a prototype of the present invention, the amount administered to a test animal based on the composition ratio (1: 3) of Aloe vera and DHA (DHA), which is proved in the results of the above animal experiment, is determined by the human amount. It prepared in conversion.

Brain Activator Prototype is 8.0 parts by weight of lyophilized powder of Aloe vera, which has been scientifically proven effective, and 24.0 parts by weight of high purity DHA isolated from fish oil (DHA: 31/0% or less). As a main component, 1.0 parts by weight of lecithin (iecithin) isolated from a soybean found as a brain aid component, and 1.0 parts by weight of tocopherol and 0.5 parts by weight of ascorbic acid were added and mixed as a natural homeostatic agent.

It is mixed and homogenized by adding 8.0 parts by weight of glucose, which is used only as brain energy, and 0.3 parts by weight of tyrosine, an amino acid component known to supplement the function of acetylcholine, a neurotransmitter component of the brain. It was.

Brain softener was prepared by using 57.2 parts by weight of cooking oil and wax as a molding agent of the soft capsule.

The storage test for the product stability of the soft capsule as a brain activator was carried out using the stability test method used to test the stability of the drug.

B. Stability Test of Prototype

The stability test of the brain activator soft capsule prototype was conducted using a test method related to drug stability and shelf life, and the product was tested for 3 months under extended conditions of 40 ° C (± 1 ° C) and 75% RH (± 5% RH). The characteristics and compositional changes of 5 lots of 20 capsules per sugar were analyzed and evaluated.

The prototype was analyzed and evaluated periodically through storage test for 3 months under 40 ℃ (± 1 ℃) and 75% RH (± 5% RH) expansion conditions. No component changes were seen in the whole lot. Therefore, since the stability of the prototype of the present invention is sufficiently recognized, the stability during the distribution period is sufficiently ensured.

Therefore, based on the physiological effects of the brain activator and the preparation of the prototype and stability test results, the brain activator soft capsule was prepared.

In particular, the soft capsule product according to the present invention is preferably prepared in the form of soft capsules for the purpose of preventing the oxidation, stability of the quality and convenience of taking since the growth of the compounding ingredients is unsaturated, oily and syrup-like soft capsules.

Hereinafter, an embodiment of the preparation method of the brain active agent soft capsule of the present invention is as follows.

EXAMPLE

It is a high-purity DeHy made by extracting the active ingredient of Aloe vera, which is not only excellent natural medicinal effect, but also a well-known health food, extracted from 8.0 parts by weight of lyophilized powder and back blue fish. 24.0 parts by weight of DHA was used as the main raw material, and 1.0 parts by weight of lecithin isolated from soybean was added and mixed, and then, 1.0 parts by weight of tocopherol as a natural antioxidant and 0.5 parts by weight of ascorbic acid as a food enhancer and nutrition enhancer. Add, homogenize.

8.0 parts by weight of glucose, which is used only as brain energy, and 0.3 parts by weight of tyrosine as an amino acid component known to supplement the function of acetylcholine, a brain neurotransmitter, are mixed with these mixtures. , Homogenized. It is prepared by packaging 57.2 parts by weight of cooking oil and wax as a soft capsule molding agent, and packaged and shipped.

Claims (3)

Extracts the active ingredient of Aloe vera and freeze-dried powder and high-purity DHA, which is extracted and purified by conventional methods, as a main ingredient, and then extracted and refined lecithin from soybean. Then, mixed and homogenized by adding tocopherol as a natural raw material and ascorbic acid as a nutrient enhancer, and then mixing and homogenizing by adding tyrosine as an amino acid component, and then using cooking oil and wax as a molding agent. A method for producing a softening agent for brain activator, characterized in that the molding. The method of claim 1, wherein the mixing ratio of DHA-containing fish oil to Aloe vera is 0.3 to 15.0 times. According to claim 1, 0.5 to 30.0 parts by weight of Aloe vera freeze-dried powder, 1.0 to 50.0 parts by weight of DHA fish oil, 0.1 to 15 parts by weight of soy lecithin, 0.2 to 15 parts by weight of tocopherol, Corbin acid 0.2 ~ 10 parts by weight, glucose 0.5 ~ 20.0 parts by weight, tyrosine 0.05 ~ 10.0 parts by weight and cooking oil 10 to 70 parts by weight of the preparation method of the brain active agent soft capsule characterized in that it is prepared.