KR0138738B1 - Oily preparation and method thereof - Google Patents

Abstract

The present invention provides an oil preparation having a high activity of an antioxidant, which is an active ingredient, and a high penetration ability into cells within a diseased area, and a method for preparing the oil, wherein the oil preparation includes rice seeding and / or wheat seeding and soybean seeding at a temperature not exceeding 100 ° C. Heating the grain raw material comprising a fine powder, it is brewed by the addition of soup, and the brewing powder is added to an oil mixture comprising the oil obtained from the sesame heated in the same way and the oil obtained from the raw sesame. The total amount of the fine powder and the oil mixture ratio of 60 to 95% by weight of the oil mixture, which is used for the prevention and treatment of various inflammations and incurable diseases caused by the destruction of cellular tissues by semi-negative oxygen and lipid peroxides produced in the human body. It is available.

Description

Oil preparation and preparation method thereof

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to oil preparation and a method for preparing the same, and more particularly, to an oil preparation including a substance which inhibits the production of reactive oxygen and lipid peroxides in the human body. Hereinafter, this substance will be referred to as an antioxidant.

Inflammation and refractory diseases caused by the destruction of defoamers caused by the reactive oxygen and lipid peroxides produced recently in the human body, such as chronic hypersection, thrombophlebitis, progressive systemic sclerosis, Burger's disease, Raynaud's disease, refractory There is a growing interest in antioxidants, which have been used to prevent and treat skin ulcers.

The present inventors referred to above in the body when the above-described antioxidants include low molecular weight substances such as flavonoids, polyphenols, tannins, tocopherols, vitamin B ₁ in the plant and can take in through the mouth with them as drugs or as a health food It was confirmed that the production of reactive oxygen and lipid peroxides can be suppressed. (Reference: Journal of Japan Pharmacist Association, No. 39. Vol. 12, Supplement, Bioavailability of SOD (Superoxide Dismtasc) and crude drug, PP-1097-1119, Published on December 1, 1987).

As a preparation containing an antioxidant, the antioxidant composition described in Japanese Unexamined Patent Publication No. 63-79834 of Niwa Seed and Plant Nutrients of Japanese Patent No. 1366268 is usually known.

Plant nutrients of No. 1366268 include roasted brown rice flour and soybean flour mixed with green tea powder; To this mixture a small amount of Koji fungus is added; Then, the powder mixture is immersed in the mixed solution of sesame oil and soybean oil for about 4 days to extract the active ingredient; Centrifugation to remove precipitates; And packing the residual oily substance into capsules made of gelatin and the like.

The antioxidant composition of 63-79834 is lightly heated so as not to burn or crush the plant seed or its seed; Microorganisms are then added to brew the heat treated plant material; And vegetable oils obtained from plants that have also been slightly heat-treated to the streets. Optionally, vitamin C, vitamin C derivatives or plants containing them may be added to the composition.

Plant seeds include seeds containing antioxidants such as flavonoids, polyphenols, tannins, cotoperols, vitamin B ₁ and the like, such as rice, wheat, barley, soybeans, adzuki bean, corn, and veins. Peas are used. Vegetable oils include soybean oil, sesame oil, cottonseed oil, corn oil, safflower oil, evening primrose oil, rice bran oil, flat oil and olive oil.

However, the plant nutrient of 1366268 has a low antioxidant activity because it is a method of processing plant raw materials.

Niwa's 63-79834 antioxidant composition weakly heats the raw plant material to avoid burning or crushing it in the far infrared, thus preventing the desulfurization of antioxidants due to high temperatures, and glass-converting the raw material to low molecular weight active ingredients, and This problem was avoided by significantly increasing the activity of the antioxidants as the heat treatment material was brewed to further freely convert to low molecular weight active ingredients.

However, subsequent studies by the present inventors have confirmed that although the above-mentioned antioxidants significantly inhibit the production of reactive oxygen and lipid peroxides in the test tube, the antioxidant activity is not sufficiently expressed in the human body.

In order for antioxidants to reach inside cells suffering from diseases such as inflammation caused by reactive oxygen and lipid peroxides, it is necessary to pass them through the cell membrane covering the surface of the cells. However, human cell membranes are rich in oils and fats, so that only oily substances are allowed to pass. Therefore, the above-mentioned antioxidant composition having a low amount of oily substance has a low ability to penetrate into the cells through the cell membranes.

Therefore, the present inventors continued to improve the cell wall passage ability of the antioxidant composition by further improving in the selection of a plant species as a raw material and a manufacturing method thereof. As a result, an oil preparation having high antioxidative activity as an active ingredient and having high permeability into cells suffering from diseases was obtained and completed the present invention.

It is an object of the present invention to provide an oil preparation having high antioxidative activity as an active ingredient and a high penetration force into a cell at a disease site and a preparation method thereof.

The method for producing the oil preparation of the present invention comprises heating rice seedlings and / or wheat seedlings and soybeans at a temperature not exceeding 100 ° C; Koji is added to this heated material; Brewing these mixtures; The mixture is then powdered; And adding the steamed powder mixture to an oil mixture comprising oil obtained from sesame and oil obtained from sesame seeds heated at a temperature not exceeding 100 ° C., wherein the powder mixture and oil mixture The ratio of oil mixture to the total amount is 60 to 95% by weight.

In addition to the rice seedlings, wheat seedlings and soybeans, the plant species described in Japanese Unexamined Patent Application Publication No. 63-79834, ie, barley, red bean beans, corn, veins, peas, etc., are rich in antioxidants. Although it can also be discussed, our studies have found that rice seedlings, wheat seedlings and soybeans are the most desirable materials.

Rice seedlings, wheat seedlings and soybeans are followed by rice bran, veins and wheat. Thus, at least one of rice bran, veins and wheat as raw materials may be included with rice seedlings and / or wheat seedlings. In any case, it is preferable that the proportion of seed (rice seed and / or wheat seed) in the grain raw material is at least 1% or 1% by weight.

The antioxidants in the above-mentioned grain raw materials, together with other substances, form complex macromolecular structure polymers, and thus have no activity on their own. Therefore, the macromolecules may be macromolecules by means of light heating such that they are separated into low molecular weight antioxidants. It is necessary to cut the chains. However, if the heating temperature is too high, low molecular weight antioxidants are deactivated, so it is necessary to select the heating conditions in consideration of this fact.

In order to satisfy such conditions, it is necessary to heat the grain material for a sufficiently long time at a temperature not exceeding 100 ° C. Specifically, the grain raw material is preferably placed in a ceramic container such as pottery, which emits far infrared rays having a wavelength of 4 to 14 μm, and gradually stirs while maintaining a temperature of about 90 to 96 ° C. to proceed with heating.

Since the heating time depends on the type of grain species, it cannot be defined uniformly, but is preferably about 30 minutes to 3 hours. Incidentally, the heating method is not limited to the above-described method as long as the antioxidant material in the grain raw material can be sufficiently separated into the low molecular material to prevent its desulfurization.

After the heat treatment described above is added to the raw material (Aspergillus orizae) to brew. Brewing conditions are good at about 2-6 days at 20-36 degreeC. Brewing is about 2 to 3 hours is sufficient when carried out using a fermentor. This brewing operation is carried out to further promote the dissociation and conversion of the antioxidants in the grain raw material to low molecular weight substances. Through this brewing step, the activity of the antioxidants is dramatically increased compared to the raw materials before the heat treatment.

The brewed material is then ground to fine powder. Grinding can be performed using a commercially available mill. However, some types of mills generate high temperatures during use, which deoxidize antioxidants. Therefore, it is preferable to use one that does not generate high temperature in use, for example, to use a mill mill.

Next, an oil obtained from heated sesame seeds (hereinafter referred to as sesame paste oil) is prepared by mixing an oil obtained from raw sesame seeds in an appropriate ratio, and the fine powder described above is added to the oil mixture. Sesame paste oil is an oil obtained by gradually heating the raw sesame seeds and grinding and pressing them for a sufficiently long time at a temperature not exceeding 100 ° C., but the fine solids formed by grinding sesame seeds remain intact and their appearance is paste-like. This sesame paste oil contains abundantly low molecular weight antioxidants and can be used to obtain oil preparations having high activity antioxidants.

However, this sesame paste oil has a high viscosity and a large oil droplet size, so that the preparation containing only the sesame paste oil in the fine powder described above has poor penetrability into the cells of the diseased area. However, when sesame oil obtained from raw sesame seeds is added to sesame paste oil, the size of oil droplets can be reduced and the penetration of diseased areas into cells is improved.

Oil collected from raw sesame seeds is a conventional sesame oil that is commercially acceptable as an oil obtained by powdering raw sesame seeds, pressing them, and removing solids. The mixing ratio of sesame paste oil to conventional sesame oil cannot be defined uniformly since it also depends on the amount of fine powder added. Preferably, the sesame paste oil is a ratio of 1 to 3 parts by weight based on 1 part by weight of sesame paste oil.

The ratio of the oil mixture to the fine powder added thereto causes the mixed oil to be 60 to 95% by weight of the total amount of both. If the oil mixture is 60% by weight or less, the ability to pass through the cell membrane is poor, on the contrary, if it exceeds 95% by weight, the concentration of antioxidants as an active ingredient is low, and the inhibitory effect of the production of reactive oxygen and lipid peroxide is also reduced.

The oil preparation according to the present invention to be ingested through the oral cavity may be prepared by adding a fine powder therein and gelatin capsules as soon as possible as the oil mixture is made. However, the material mixture may preferably be aged at 20 to 35 ° C. for about 3 to 30 days, more preferably at 28 to 30 ° C. for about 1 week before encapsulation.

By applying the aging treatment, the free and low molecular weight conversion of the antioxidants is further advanced by Koji, which remains in the fine powder, and as a result, the intracellular activity and the osmotic ability of the reactive component are further improved.

The oil preparation of the present invention may also comprise grain raw materials comprising rice seedlings or wheat seedlings and soybeans and optionally at least one rice bran, vein or wheat, which is heated as described above as a fine powder without heating and brewing. And the brewed fine powder is added to the oil mixture.

In fine powders of grain raw materials that have not been heated and brewed, the antioxidants as active ingredients do not dissociate and convert into low molecular weight substances. However, when added to oil mixtures with heated and brewed micropowders, the dissociation of antioxidants and conversion to low molecular weights gradually progress even after encapsulation, due to the Koji remaining in the brewed micropowders. The effect of inhibiting the production of oxygen and lipid peroxides has the long lasting advantage.

On the contrary, the oil preparation using only the heated and brewed fine powder gradually degrades the antioxidant as an active ingredient due to the enzymatic reaction which proceeds even after encapsulation. As a result, the expression period is shorter than that of the addition of the untreated fine powder. .

However, when the amount of untreated fine powder is excessive, the activity of the active ingredient is reduced. Therefore, the amount of untreated fine powder is preferably about 0.5 to 1 parts by weight based on 1 part by weight of heated and brewed fine powder. In addition, even in this case including two types of fine powder, the oil mixture is 60 to 95% by weight based on the total amount.

In this oil preparation obtained according to the present invention, both the activity of the antioxidant and the osmotic power into the cells are high, and it is evident from the clinical test results described below, but various inflammations in which the therapeutic effect is insufficient as a conventional anti-inflammatory agent. And excellent effect against intractable disease.

The oil preparation of the present invention can be encapsulated with gelatin and the like and is taken as a medicine through the oral cavity. In addition, in the case of metamorphic pigmentation such as dermatitis, chloasma, freckles, or wrinkles, an oil preparation may be applied directly to the corresponding site. In addition to dermatitis and the like, the oil preparation of the present invention can also be applied to adult diseases and intractable diseases such as chronic arthritis, thrombophlebitis, progressive systemic sclerosis, Burger disease, Raynaud's disease, refractory skin ulcers and the like. The preparation of the present invention is also effective in the treatment and prevention of diseases, burns, trauma, fatigue, hangover, constipation, etc. resulting from various other pollutions.

In addition, since the oil preparation of the present invention uses only grain, koji and sesame as raw materials, there are no side effects, and as a result, it may be taken as a healthy food for maintaining and certifying health. Needless to say, when preparing a preparation, supplementary supplements or various vitamin oils, minerals, etc., and ingredients useful for health, such as flavorings, seasonings, colorings, etc., may optionally be added.

Example

One part of each rice seed, soybean, rice bran, vein and wheat was placed in a pot of 4-14 μm far-infrared infrared rays, and heated at a temperature of 90-96 ° C. for 3 hours while slowly stirring to prevent burning or pressing. Next, Koji was added at 3% of the total amount and brewed at a temperature of 36 to 40 ° C. for 72 hours, and this brewing material was made into fine powder using a millstone. The five kinds of grain raw materials mentioned above were ground to millstone without heating or brewing to obtain the same amount of fine powder. Meanwhile, sesame seeds were heated in the same way as the grain material, milled and pressed to obtain sesame paste oil.

Next, 48.9 parts by weight of commercially used sesame oil was mixed with 28.1 parts by weight of the sesame paste oil, and 11.5 parts by weight of heat-brewed fine powder and 11.5 parts by weight of untreated fine powder were added and then mixed uniformly. The resulting mixture was aged at 28 ° C. for 1 week and then encapsulated in gelatin to give an oil preparation.

1. Test tube test

(1) To test the oil's arrival and permeability, the oil preparation of this Example is reacted with unsaturated fatty acid (docosahexaenoic acid) reacted with reactive oxygen generated by ultraviolet rays to form TBA ( Thiobarbituric acid (thiobarbituric acid) was added to the reaction system and the formation of lipid peroxide was inhibited.

That is, 1.8 mg / ml of the oil preparation test sample of this invention was added to 0.1 microliters of dicosahexanoic acid diluted 100-fold, and the produced lipid peroxide was measured by TBA reaction. In a TBA reaction, 0.2 ml of 7% sodium dodecyl sulfate, 2 ml of 0.1N HCI and 0.3 ml of phosphotungstic acid were added thereto, followed by addition of 1.8 mg / ml of test sample, followed by 0.67% TBA with acetic acid. 1 ml of the reagent mixed in a 1: 1 ratio was added, and the excitation at 515 nm and the emission at 553 nm were measured using a fluorescence spectrophotometer.

As a comparative example, the plant nutrients (Comparative Example 1) of Japanese Patent No. 1366268 and the antioxidant composition (Comparative Example 2) described in Japanese Unexamined Patent Application Publication No. 63-79834 were used, and the measurement was carried out in the same manner. The results are summarized in Table 1.

TABLE 1

Test results

Although any of the test samples significantly inhibited the production of lipid peroxides (TBA reactive substances) from unsaturated fatty acids (docosahexanoic acid) by ultraviolet irradiation ( ¹ O ₂ ) irradiation, in particular the oil preparation of the present invention It is excellent in suppressing the production (P <0.0001). This result supports the fact that the oil preparation of the present invention has a high activity of the antioxidant substance and has a high penetration ability into the cell causing disease (Comparative Examples 1 and 2).

(2) A low molecular weight antioxidant contained in the oil preparation of the present invention was prepared from [3,4- ³ H ₂ ] -antioxidant labeled with isotope ( ³ H), which was added into human cells in a test tube, And [ ³ H ₂ ] bound number (cpm) on the cell wall was measured with a scintillation counter, whereby the ability to reach the cell wall was measured (labeled trithiated thymidine was 2Cl / mM).

The same test was conducted with Comparative Examples 1 and 2 as well. The results are summarized in Table 2. As apparent from Table 2, the oil preparation of the present invention had a very high affinity for cell walls as compared with Comparative Examples 1 and 2.

TABLE 2

(3) The oil preparation of the present invention was sonicated and added to a reactive oxygen generation system (neutrophyll and xanthine-xanthine oxidase) to give a conversion bioconcentration of 1.6 mg / ml, and three types of reactivity. Oxygen (O _2- , H ₂ O ₂ and OH-) was measured in comparison with no addition (control). The converted bioconcentration is the amount assumed to be present in the blood when the amount normally taken per day (9 g for the oil preparation of the present invention) is absorbed in vivo.

The measurement methods for the three types of reactive oxygen are as follows.

As for O ₂ −, a method of measuring the amount of reduction of O ₂ − by Perititch C at 550 nm using a spectrophotometer and converting it into the amount of O ₂ − was used.

For H ₂ O ₂ , the development of 370 nm produced by Hadachi Corporation using scopoline and peroxidase based on the fact that H ₂ O ₂ reduces the fluorescence produced by scopoletin in the presence of peroxidase and The degree of fluorescence reduction of scopoletin was measured using a spectrophotometer with an emission of 460 nm.

About OH- Based on the principle that keto-methol-butyl acid (KMB) reacts with OH- to produce ethylene gas, a method of converting ethylene gas into OH- by quantifying gas chromatography produced by Hitachi Corporation was used. The same test was performed for Comparative Examples 1 and 2 as well. The results are summarized in Table 3 below.

TABLE 3

Test results

The antioxidant effect of the oil preparation of the present invention was somewhat inferior to Comparative Example 2, but showed a stronger antioxidant effect than Comparative Example 1. However, as is clear from Tables 1 and 2, Comparative Example 2 has a lower oil content, and as a result, the ability to reach disease-causing cells is low.

Therefore, when judged as a whole, the oil preparation of the present invention is the most excellent as a sulfate agent because of its high antioxidant effect and very high penetration into the diseased cell.

2. Clinical test

Patients with autoimmune diseases, such as chronic arthritis, blood circulation failure, nephritis, cirrhosis, liver circumference, freckles, etc., which have so far resisted or worsened against any of the bisteroid anti-inflammatory agents, steroids and the antioxidant composition of Comparative Example 2 96 The effects of treatment were investigated for the population. The results are summarized in Table 4.

TABLE 4

Claims (6)

Heating to a temperature not exceeding 100 ° C. of cereals consisting of rice seedlings and / or wheat seedlings and soybeans; Brewing by adding Koji to the heated material; Powdered brewed material; And a powdered material is added to an oil mixture comprising a paste oil obtained from sesame seeds heated at a temperature not exceeding 100 ° C., the oil mixture also comprising an oil obtained from raw sesame seeds; And an oil ratio of 60 to 95% by weight relative to the total amount of the powdered material and the oil mixture. The oil preparation according to claim 1, further comprising a grain seed consisting of rice seedlings and / or wheat seedlings and soybeans in fine powder without heating and brewing. 3. The oil preparation according to claim 1 or 2, wherein the heated or unheated cereal raw material also comprises at least one of rice bran, vein or wheat in addition to rice seed and / or wheat seed and soybean. Heating to a temperature not exceeding 100 ° C. consisting of rice seed and / or wheat seed and soybeans; Brewing by adding Koji to the heated material; Powdering the brewed material; And adding a powdered material to an oil mixture comprising a paste oil obtained from a sesame heated from a temperature not exceeding 100 ° C., wherein the oil mixture is prepared from raw sesame seeds. Also comprising the oil obtained; And 60 to 95% by weight of the oil mixture with respect to the total amount of the powdered material and the oil mixture, and is heated using far infrared rays having a wavelength of 4 to 14 μm when the grain material is heated. Process for preparing an oil preparation. Heating to a temperature not exceeding 100 ° C. consisting of rice seed and / or wheat seed and soybeans; Brewing by adding Koji to the heated material; Powdering the brewed material; And adding a powdered material to an oil mixture comprising a paste oil obtained from a sesame heated from a temperature not exceeding 100 ° C., wherein the oil mixture is prepared from raw sesame seeds. Also comprising the oil obtained; And an oil ratio of 60 to 95% by weight based on the total amount of the powdered material and the oil mixture, and brewing the heat-treated grain raw material at 20 to 36 ° C. for 2 to 6 days. Manufacturing method. Heating to a temperature not exceeding 100 ° C. consisting of rice seed and / or wheat seed and soybeans; Brewing by adding Koji to the heated material; Powdering the brewed material; And adding a powdered material to an oil mixture comprising a paste oil obtained from a sesame heated from a temperature not exceeding 100 ° C., wherein the oil mixture is prepared from raw sesame seeds. Also comprising the oil obtained; And a ratio of the oil mixture to the total amount of the powdered material and the oil mixture is 60 to 95% by weight, and the fine powder is aged at 20 to 35 ° C. for 3 to 30 days to be added to the oil mixture. Process for preparing oil preparation.