JPWO2022087150A5 - - Google Patents
Download PDFInfo
- Publication number
- JPWO2022087150A5 JPWO2022087150A5 JP2023524116A JP2023524116A JPWO2022087150A5 JP WO2022087150 A5 JPWO2022087150 A5 JP WO2022087150A5 JP 2023524116 A JP2023524116 A JP 2023524116A JP 2023524116 A JP2023524116 A JP 2023524116A JP WO2022087150 A5 JPWO2022087150 A5 JP WO2022087150A5
- Authority
- JP
- Japan
- Prior art keywords
- sequence
- nucleic acid
- polynucleotide
- hybridization
- complementary
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 230000000295 complement effect Effects 0.000 claims 52
- 150000007523 nucleic acids Chemical class 0.000 claims 41
- 108091033319 polynucleotide Proteins 0.000 claims 34
- 102000040430 polynucleotide Human genes 0.000 claims 34
- 239000002157 polynucleotide Substances 0.000 claims 34
- 102000039446 nucleic acids Human genes 0.000 claims 31
- 108020004707 nucleic acids Proteins 0.000 claims 31
- 238000009396 hybridization Methods 0.000 claims 28
- 238000012163 sequencing technique Methods 0.000 claims 26
- 239000012634 fragment Substances 0.000 claims 20
- 238000000034 method Methods 0.000 claims 10
- 108091034117 Oligonucleotide Proteins 0.000 claims 6
- 230000000903 blocking effect Effects 0.000 claims 6
- 108091093088 Amplicon Proteins 0.000 claims 3
- OPTASPLRGRRNAP-UHFFFAOYSA-N cytosine Chemical class NC=1C=CNC(=O)N=1 OPTASPLRGRRNAP-UHFFFAOYSA-N 0.000 claims 3
- 239000002773 nucleotide Substances 0.000 claims 3
- 125000003729 nucleotide group Chemical group 0.000 claims 3
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 claims 2
- 102000054766 genetic haplotypes Human genes 0.000 claims 2
- 230000000977 initiatory effect Effects 0.000 claims 2
- 238000003780 insertion Methods 0.000 claims 2
- 230000037431 insertion Effects 0.000 claims 2
- 239000007787 solid Substances 0.000 claims 2
- 229930024421 Adenine Natural products 0.000 claims 1
- GFFGJBXGBJISGV-UHFFFAOYSA-N Adenine Chemical compound NC1=NC=NC2=C1N=CN2 GFFGJBXGBJISGV-UHFFFAOYSA-N 0.000 claims 1
- 108091000080 Phosphotransferase Proteins 0.000 claims 1
- 229960000643 adenine Drugs 0.000 claims 1
- 238000000137 annealing Methods 0.000 claims 1
- 239000011324 bead Substances 0.000 claims 1
- 238000003776 cleavage reaction Methods 0.000 claims 1
- 230000000865 phosphorylative effect Effects 0.000 claims 1
- 102000020233 phosphotransferase Human genes 0.000 claims 1
- 230000007017 scission Effects 0.000 claims 1
- 238000010008 shearing Methods 0.000 claims 1
- 230000002194 synthesizing effect Effects 0.000 claims 1
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US202063094422P | 2020-10-21 | 2020-10-21 | |
| US63/094,422 | 2020-10-21 | ||
| US202163256040P | 2021-10-15 | 2021-10-15 | |
| US63/256,040 | 2021-10-15 | ||
| PCT/US2021/055878 WO2022087150A2 (en) | 2020-10-21 | 2021-10-20 | Sequencing templates comprising multiple inserts and compositions and methods for improving sequencing throughput |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2023547366A JP2023547366A (ja) | 2023-11-10 |
| JPWO2022087150A5 true JPWO2022087150A5 (enrdf_load_stackoverflow) | 2024-10-29 |
Family
ID=78622058
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2023524116A Pending JP2023547366A (ja) | 2020-10-21 | 2021-10-20 | 複数のインサートを含む配列決定鋳型、並びに配列決定スループットを改善するための組成物及び方法 |
Country Status (10)
Families Citing this family (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US11859241B2 (en) | 2021-06-17 | 2024-01-02 | Element Biosciences, Inc. | Compositions and methods for pairwise sequencing |
| EP4355913A1 (en) * | 2021-06-17 | 2024-04-24 | Element Biosciences, Inc. | Compositions and methods for pairwise sequencing |
| US11220707B1 (en) | 2021-06-17 | 2022-01-11 | Element Biosciences, Inc. | Compositions and methods for pairwise sequencing |
| WO2022266470A1 (en) * | 2021-06-17 | 2022-12-22 | Element Biosciences, Inc. | Compositions and methods for pairwise sequencing |
| WO2023168300A1 (en) * | 2022-03-01 | 2023-09-07 | Guardant Health, Inc. | Methods for analyzing cytosine methylation and hydroxymethylation |
| AU2023236924A1 (en) | 2022-03-15 | 2024-10-24 | Illumina, Inc. | Paired-end sequencing |
| CA3223669A1 (en) | 2022-03-15 | 2023-09-21 | Niall Gormley | Concurrent sequencing of forward and reverse complement strands on concatenated polynucleotides for methylation detection |
| WO2023230556A2 (en) * | 2022-05-26 | 2023-11-30 | Illumina, Inc. | Preparation of long read nucleic acid libraries |
| EP4590854A1 (en) | 2022-09-19 | 2025-07-30 | Illumina, Inc. | Deformable polymers comprising immobilised primers |
| WO2024213788A1 (en) * | 2023-04-13 | 2024-10-17 | Aniling Sl. | Method of dna sequencing |
| WO2024256580A1 (en) | 2023-06-14 | 2024-12-19 | Illumina, Inc. | Concurrent sequencing with spatially separated rings |
| WO2024256581A1 (en) | 2023-06-14 | 2024-12-19 | Illumina, Inc. | Determination of modified cytosines |
| WO2025062001A1 (en) | 2023-09-20 | 2025-03-27 | Illumina, Inc. | Optimised nucleic acid sequencing |
| WO2025062002A1 (en) | 2023-09-20 | 2025-03-27 | Illumina, Inc. | Concurrent sequencing using nick translation |
Family Cites Families (39)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA2044616A1 (en) | 1989-10-26 | 1991-04-27 | Roger Y. Tsien | Dna sequencing |
| EP1591541B1 (en) | 1997-04-01 | 2012-02-15 | Illumina Cambridge Limited | Method of nucleic acid sequencing |
| AR021833A1 (es) | 1998-09-30 | 2002-08-07 | Applied Research Systems | Metodos de amplificacion y secuenciacion de acido nucleico |
| US7955794B2 (en) | 2000-09-21 | 2011-06-07 | Illumina, Inc. | Multiplex nucleic acid reactions |
| AU2001282881B2 (en) | 2000-07-07 | 2007-06-14 | Visigen Biotechnologies, Inc. | Real-time sequence determination |
| AU2002227156A1 (en) | 2000-12-01 | 2002-06-11 | Visigen Biotechnologies, Inc. | Enzymatic nucleic acid synthesis: compositions and methods for altering monomer incorporation fidelity |
| US7057026B2 (en) | 2001-12-04 | 2006-06-06 | Solexa Limited | Labelled nucleotides |
| EP3587433B1 (en) | 2002-08-23 | 2020-04-22 | Illumina Cambridge Limited | Modified nucleotides |
| EP1701785A1 (en) | 2004-01-07 | 2006-09-20 | Solexa Ltd. | Modified molecular arrays |
| US7476503B2 (en) | 2004-09-17 | 2009-01-13 | Pacific Biosciences Of California, Inc. | Apparatus and method for performing nucleic acid analysis |
| GB0427236D0 (en) | 2004-12-13 | 2005-01-12 | Solexa Ltd | Improved method of nucleotide detection |
| US7405281B2 (en) | 2005-09-29 | 2008-07-29 | Pacific Biosciences Of California, Inc. | Fluorescent nucleotide analogs and uses therefor |
| SG170802A1 (en) | 2006-03-31 | 2011-05-30 | Solexa Inc | Systems and devices for sequence by synthesis analysis |
| ATE542921T1 (de) | 2006-04-18 | 2012-02-15 | Advanced Liquid Logic Inc | Pyrosequenzierung auf tröpfchenbasis |
| EP2089517A4 (en) | 2006-10-23 | 2010-10-20 | Pacific Biosciences California | POLYMERASEENZYME AND REAGENTS FOR ADVANCED NUCKIC ACID SEQUENCING |
| US9080211B2 (en) | 2008-10-24 | 2015-07-14 | Epicentre Technologies Corporation | Transposon end compositions and methods for modifying nucleic acids |
| EP2427572B1 (en) | 2009-05-01 | 2013-08-28 | Illumina, Inc. | Sequencing methods |
| EP2633069B1 (en) | 2010-10-26 | 2015-07-01 | Illumina, Inc. | Sequencing methods |
| US9644199B2 (en) | 2012-10-01 | 2017-05-09 | Agilent Technologies, Inc. | Immobilized transposase complexes for DNA fragmentation and tagging |
| US9683230B2 (en) | 2013-01-09 | 2017-06-20 | Illumina Cambridge Limited | Sample preparation on a solid support |
| WO2015002789A1 (en) | 2013-07-03 | 2015-01-08 | Illumina, Inc. | Sequencing by orthogonal synthesis |
| MX370616B (es) * | 2013-11-22 | 2019-12-17 | Theranos Ip Co Llc | Amplificacion de acidos nucleicos. |
| WO2015095226A2 (en) | 2013-12-20 | 2015-06-25 | Illumina, Inc. | Preserving genomic connectivity information in fragmented genomic dna samples |
| AU2015247779B2 (en) | 2014-04-15 | 2021-06-24 | Illumina, Inc. | Modified transposases for improved insertion sequence bias and increased DNA input tolerance |
| US10975371B2 (en) | 2014-04-29 | 2021-04-13 | Illumina, Inc. | Nucleic acid sequence analysis from single cells |
| CA2964799A1 (en) * | 2014-10-17 | 2016-04-21 | Illumina Cambridge Limited | Contiguity preserving transposition |
| EP3636757A1 (en) * | 2014-10-17 | 2020-04-15 | Illumina Cambridge Limited | Contiguity preserving transposition |
| US10844428B2 (en) | 2015-04-28 | 2020-11-24 | Illumina, Inc. | Error suppression in sequenced DNA fragments using redundant reads with unique molecular indices (UMIS) |
| WO2016189331A1 (en) | 2015-05-28 | 2016-12-01 | Illumina Cambridge Limited | Surface-based tagmentation |
| WO2017019278A1 (en) | 2015-07-30 | 2017-02-02 | Illumina, Inc. | Orthogonal deblocking of nucleotides |
| KR20180055905A (ko) * | 2015-10-09 | 2018-05-25 | 아큐라젠 홀딩스 리미티드 | 증폭 산물의 농축을 위한 방법 및 조성물 |
| WO2018108328A1 (en) * | 2016-12-16 | 2018-06-21 | F. Hoffmann-La Roche Ag | Method for increasing throughput of single molecule sequencing by concatenating short dna fragments |
| EP3571616B1 (en) | 2017-01-18 | 2021-05-19 | Illumina, Inc. | Methods and systems for generation and error-correction of unique molecular index sets with heterogeneous molecular lengths |
| SG11201810639RA (en) | 2017-02-21 | 2018-12-28 | Illumina Inc | Tagmentation using immobilized transposomes with linkers |
| AU2018261332B2 (en) | 2017-05-01 | 2024-12-05 | Illumina, Inc. | Optimal index sequences for multiplex massively parallel sequencing |
| DK3622089T3 (da) | 2017-05-08 | 2024-10-14 | Illumina Inc | Fremgangsmåde til sekventering under anvendelse af universelle korte adaptere til indeksering af polynukleotidprøver |
| US11447818B2 (en) | 2017-09-15 | 2022-09-20 | Illumina, Inc. | Universal short adapters with variable length non-random unique molecular identifiers |
| AU2018375785A1 (en) | 2017-11-30 | 2019-12-12 | Illumina, Inc. | Validation methods and systems for sequence variant calls |
| WO2020014437A1 (en) | 2018-07-12 | 2020-01-16 | Levine Alison | Modular apparel |
-
2021
- 2021-10-20 KR KR1020237016082A patent/KR20230091116A/ko active Pending
- 2021-10-20 AU AU2021366658A patent/AU2021366658A1/en active Pending
- 2021-10-20 MX MX2023004461A patent/MX2023004461A/es unknown
- 2021-10-20 CN CN202180071179.8A patent/CN116438319A/zh active Pending
- 2021-10-20 JP JP2023524116A patent/JP2023547366A/ja active Pending
- 2021-10-20 WO PCT/US2021/055878 patent/WO2022087150A2/en active Application Filing
- 2021-10-20 CA CA3198842A patent/CA3198842A1/en active Pending
- 2021-10-20 EP EP21807406.0A patent/EP4232600A2/en active Pending
- 2021-10-20 IL IL302207A patent/IL302207A/en unknown
-
2023
- 2023-04-20 US US18/303,905 patent/US20230407388A1/en active Pending
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| JP2703183B2 (ja) | 複数標的の同時増幅 | |
| EP2691546B1 (en) | Identification of a nucleic acid template in a multiplex sequencing reaction | |
| KR101243266B1 (ko) | 이중 특이성 올리고뉴클레오타이드를 사용한 방법 및 이중 특이성 올리고뉴클레오타이드 | |
| KR101534228B1 (ko) | 이중 특이성 올리고뉴클레오타이드를 사용한 방법 및 이중 특이성 올리고뉴클레오타이드 | |
| JP4263612B2 (ja) | アニーリング調節プライマーおよびその使用 | |
| JPWO2022087150A5 (enrdf_load_stackoverflow) | ||
| US20200299764A1 (en) | System and method for transposase-mediated amplicon sequencing | |
| JP2005511096A6 (ja) | アニーリング調節プライマーおよびその使用 | |
| EP3555316B1 (en) | Modified multiplex and multistep amplification reactions and reagents therefor | |
| JP2007125014A (ja) | 遺伝子メチル化検査法対照物 | |
| JP2007530026A (ja) | 核酸配列決定 | |
| EP3601611B1 (en) | Polynucleotide adapters and methods of use thereof | |
| CA3094054C (en) | Methods for amplification of nucleic acids with endonuclease-mediated shifting equilibrium (em-seq) | |
| KR20220063169A (ko) | 폴리뉴클레오타이드 분자 집단의 생성 방법 | |
| JP4942160B2 (ja) | ＲｅｃＡタンパク質を利用した核酸の等温増幅法 | |
| WO2023081373A1 (en) | Using hairpin formation to identify dna and rna sequences having a target nucleic acid sequence | |
| JP2024530352A (ja) | 標的ポリヌクレオチドの配列を分析するための方法 | |
| JP2001136965A (ja) | 核酸配列の増幅方法 | |
| CN111511927A (zh) | 核酸扩增中的可逆热力学陷阱(热陷阱) |