JPWO2021154888A5 - - Google Patents
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- JPWO2021154888A5 JPWO2021154888A5 JP2022545377A JP2022545377A JPWO2021154888A5 JP WO2021154888 A5 JPWO2021154888 A5 JP WO2021154888A5 JP 2022545377 A JP2022545377 A JP 2022545377A JP 2022545377 A JP2022545377 A JP 2022545377A JP WO2021154888 A5 JPWO2021154888 A5 JP WO2021154888A5
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- JP
- Japan
- Prior art keywords
- exosome
- vesicle
- chimeric
- moiety
- localization
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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- 210000001808 exosome Anatomy 0.000 claims 17
- 230000025487 vesicle localization Effects 0.000 claims 12
- 230000001086 cytosolic effect Effects 0.000 claims 3
- 101710193358 Calsyntenin-1 Proteins 0.000 claims 2
- 102100028801 Calsyntenin-1 Human genes 0.000 claims 2
- 102000000510 Integrin alpha3 Human genes 0.000 claims 2
- 108010041357 Integrin alpha3 Proteins 0.000 claims 2
- 102000012355 Integrin beta1 Human genes 0.000 claims 2
- 108010022222 Integrin beta1 Proteins 0.000 claims 2
- 102100033493 Interleukin-3 receptor subunit alpha Human genes 0.000 claims 2
- 101710123866 Interleukin-3 receptor subunit alpha Proteins 0.000 claims 2
- 239000000232 Lipid Bilayer Substances 0.000 claims 2
- 102100034925 P-selectin glycoprotein ligand 1 Human genes 0.000 claims 2
- 108010054395 P-selectin ligand protein Proteins 0.000 claims 2
- 210000004027 cell Anatomy 0.000 claims 2
- 238000004519 manufacturing process Methods 0.000 claims 2
- 102100038225 Lysosome-associated membrane glycoprotein 2 Human genes 0.000 claims 1
- 101710116771 Lysosome-associated membrane glycoprotein 2 Proteins 0.000 claims 1
- 102000000471 Prostaglandin F receptors Human genes 0.000 claims 1
- 108050008995 Prostaglandin F receptors Proteins 0.000 claims 1
- 102000001708 Protein Isoforms Human genes 0.000 claims 1
- 108010029485 Protein Isoforms Proteins 0.000 claims 1
- 108010076504 Protein Sorting Signals Proteins 0.000 claims 1
- 108091005906 Type I transmembrane proteins Proteins 0.000 claims 1
- 239000001963 growth medium Substances 0.000 claims 1
- 239000003112 inhibitor Substances 0.000 claims 1
- 108020004707 nucleic acids Proteins 0.000 claims 1
- 102000039446 nucleic acids Human genes 0.000 claims 1
- 150000007523 nucleic acids Chemical class 0.000 claims 1
- 239000008194 pharmaceutical composition Substances 0.000 claims 1
- 239000000546 pharmaceutical excipient Substances 0.000 claims 1
- 125000002924 primary amino group Chemical group [H]N([H])* 0.000 claims 1
- 102000035087 single-pass transmembrane proteins Human genes 0.000 claims 1
- 108091005496 single-pass transmembrane proteins Proteins 0.000 claims 1
Claims (9)
- キメラ小胞局在化部分を含む非天然発生のエクソソームであって、
ａ．第１の小胞局在化部分の表面－膜貫通ドメインと、
ｂ．第２の小胞局在化部分の細胞質ドメインとを含み、
前記第１の小胞局在化部分は、リソソーム関連膜タンパク質２、アイソフォームＢ（ＬＡＭＰ２Ｂ）であり、
前記第２の小胞局在化部分は、カルシンテニン１（ＣＬＳＴＮ１）、インターロイキン３受容体サブユニットアルファ（ＩＬ３ＲＡ）、インテグリンアルファ３（ＩＴＧＡ３）、インテグリンベータ１（ＩＴＧＢ１）、プロスタグランジンＦ２受容体インヒビタ（ＰＴＧＦＲＮ）、およびＰ－セレクチン糖タンパク質リガンド１（ＳＥＬＰＬＧ）からなる群から選択される、
エクソソーム。 - 前記単一パス膜貫通タンパク質は、Ｉ型膜貫通タンパク質である、請求項１に記載のエクソソーム。
- 前記キメラ小胞局在化部分は、成熟キメラ小胞局在化部分である、請求項１に記載のエクソソーム。
- 前記キメラ小胞局在化部分は、前記第１の小胞局在化部分の表面－膜貫通ドメインに先行するシグナルペプチドを含む、新生のまたは完全長キメラ小胞局在化部分から得られる、請求項１に記載のエクソソーム。
- 前記キメラ小胞局在化部分は、前記エクソソームに組み込まれ、前記エクソソームは脂質二重層と内腔で構成され、前記キメラ小胞局在化部分は、前記エクソソームの外側のアミノ表面ドメイン、前記エクソソームの前記脂質二重層における膜貫通ドメイン、および前記エクソソームの前記内腔におけるカルボキシ末端細胞質ドメインを有したトポロジを有する、請求項１に記載のエクソソーム。
- 請求項１に記載のエクソソームを産生する方法であって、
ａ．産生細胞において、請求項１に記載の第１の小胞局在化部分の表面－膜貫通ドメインと、請求項１に記載の第２の小胞局在化部分の細胞質ドメインとを含む前記キメラ小胞局在化部分をコードする核酸を発現するステップと、
ｂ．前記キメラ小胞局在化部分を含むエクソソームを単離するステップであって、前記エクソソームが、前記産生細胞によって培地中に分泌されるステップとを含む
方法。 - 請求項１に記載のエクソソームと、１つまたは複数の薬学的に許容される賦形剤とを含む薬剤組成物。
- 請求項１に記載のエクソソームと、取扱説明とを含むキット。
- 前記第１の小胞局在化部分はＬＡＭＰ２Ｂであり、前記第２の小胞局在化部分はＰＴＧＦＲＮである、請求項１に記載のエクソソーム。
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US202062966487P | 2020-01-27 | 2020-01-27 | |
| US62/966,487 | 2020-01-27 | ||
| PCT/US2021/015334 WO2021154888A1 (en) | 2020-01-27 | 2021-01-27 | Non-naturally occurring vesicles comprising a chimeric vesicle localization moiety, methods of making and uses thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2023514975A JP2023514975A (ja) | 2023-04-12 |
| JPWO2021154888A5 true JPWO2021154888A5 (ja) | 2024-02-02 |
Family
ID=77078486
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2022545377A Pending JP2023514975A (ja) | 2020-01-27 | 2021-01-27 | キメラ小胞局在化部分を含む非天然発生の小胞、その産生方法、およびその使用 |
Country Status (5)
| Country | Link |
|---|---|
| US (2) | US20230097907A1 (ja) |
| EP (1) | EP4097474A4 (ja) |
| JP (1) | JP2023514975A (ja) |
| CA (1) | CA3165930A1 (ja) |
| WO (1) | WO2021154888A1 (ja) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2023076418A2 (en) * | 2021-10-26 | 2023-05-04 | Entelexo Biotherapeutics, Inc. | Platforms, compositions, and methods for therapeutic delivery |
| WO2023097268A2 (en) * | 2021-11-23 | 2023-06-01 | Mantra Bio, Inc. | Extracellular vesicles comprising non-naturally occurring modular rna hairpins and uses thereof |
| CN115047186B (zh) * | 2022-06-15 | 2023-02-14 | 暨南大学 | 一种外泌体检测方法 |
Family Cites Families (22)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| MXPA05006579A (es) | 2002-12-19 | 2005-12-14 | Johnson & Johnson | Metodo para tratar el crecimiento de tejido angiogenico. |
| WO2005001092A2 (en) | 2003-05-20 | 2005-01-06 | Wyeth | Compositions and methods for diagnosing and treating cancers |
| ES2749393T3 (es) | 2009-04-17 | 2020-03-20 | Univ Oxford Innovation Ltd | Composición para entrega de material genético |
| US9777042B2 (en) | 2011-12-15 | 2017-10-03 | Morehouse School Of Medicine | Method of purifying HIV/SIV Nef from exosomal fusion proteins |
| CN104427986A (zh) | 2012-07-11 | 2015-03-18 | 诺华股份有限公司 | 治疗胃肠道基质瘤的方法 |
| US10231997B2 (en) * | 2012-08-15 | 2019-03-19 | The University Of Chicago | Exosome-based therapeutics against neurodegenerative disorders |
| US11274139B2 (en) | 2013-04-12 | 2022-03-15 | Evox Therapeutics Ltd | Therapeutic delivery vesicles |
| WO2016123556A1 (en) | 2015-01-30 | 2016-08-04 | The Johns Hopkins University | Extracellular vesicles for agent delivery |
| US10624849B2 (en) * | 2015-09-28 | 2020-04-21 | Northwestern University | Targeted extracellular vesicles comprising membrane proteins with engineered glycosylation sites |
| WO2017158160A1 (en) | 2016-03-17 | 2017-09-21 | Koninklijke Philips N.V. | Home visit assessment and decision support system |
| WO2017196175A1 (en) | 2016-05-12 | 2017-11-16 | Erasmus University Medical Center Rotterdam | A method for culturing myogenic cells, cultures obtained therefrom, screening methods, and cell culture medium. |
| WO2018112282A1 (en) | 2016-12-14 | 2018-06-21 | Ligandal, Inc. | Compositions and methods for nucleic acid and/or protein payload delivery |
| US11830583B2 (en) | 2017-01-06 | 2023-11-28 | Mantra Bio Inc. | Systems and methods for characterizing extracellular vesicle (EV) population by predicting whether the EV originates from B, T, or dendritic cells |
| CN111212632B (zh) * | 2017-08-25 | 2024-04-16 | 隆萨销售股份公司 | 使用膜蛋白制备治疗性外来体 |
| AU2018367670A1 (en) * | 2017-11-17 | 2020-05-07 | Lonza Sales Ag | Compositions of engineered exosomes and methods of loading luminal exosomes payloads |
| US20190276820A1 (en) | 2018-03-12 | 2019-09-12 | Mantra Bio, Inc. | Single extracellular vesicle multiplexed protein and rna analysis |
| US11414699B2 (en) | 2018-05-15 | 2022-08-16 | Mantra Bio, Inc. | Barcode-free single vesicle multiplexed protein and RNA analysis |
| CN113164043A (zh) | 2018-09-21 | 2021-07-23 | 奥夫博医疗创新有限公司 | 用于青光眼的组合物和方法 |
| US20210379192A1 (en) | 2019-01-25 | 2021-12-09 | Mantra Bio, Inc. | Skeletal muscle targeting moieties and uses thereof |
| US11801266B2 (en) | 2019-03-05 | 2023-10-31 | City Of Hope | Methods of using anti-MIR126 compounds |
| US20220323412A1 (en) | 2019-09-06 | 2022-10-13 | Chi-Chih Kang | Extracellular vesicle-fenretinide compositions, extracellular vesicle-c-kit inhibitor compositions, methods of making and uses thereof |
| EP4135665A2 (en) | 2020-04-13 | 2023-02-22 | Mantra Bio, Inc. | Modular binding proteins for extracellular vesicles and uses thereof |
-
2021
- 2021-01-27 EP EP21747358.6A patent/EP4097474A4/en active Pending
- 2021-01-27 WO PCT/US2021/015334 patent/WO2021154888A1/en active Search and Examination
- 2021-01-27 JP JP2022545377A patent/JP2023514975A/ja active Pending
- 2021-01-27 CA CA3165930A patent/CA3165930A1/en active Pending
- 2021-01-27 US US17/795,857 patent/US20230097907A1/en active Pending
-
2022
- 2022-08-16 US US17/888,862 patent/US11969504B2/en active Active
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