JPWO2021141970A5 - - Google Patents
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- JPWO2021141970A5 JPWO2021141970A5 JP2022541803A JP2022541803A JPWO2021141970A5 JP WO2021141970 A5 JPWO2021141970 A5 JP WO2021141970A5 JP 2022541803 A JP2022541803 A JP 2022541803A JP 2022541803 A JP2022541803 A JP 2022541803A JP WO2021141970 A5 JPWO2021141970 A5 JP WO2021141970A5
- Authority
- JP
- Japan
- Prior art keywords
- dna
- tag
- nucleic acid
- sequence
- endonuclease
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 claims description 113
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 claims description 113
- 108020004414 DNA Proteins 0.000 claims description 108
- 108090000765 processed proteins & peptides Proteins 0.000 claims description 108
- 150000007523 nucleic acids Chemical class 0.000 claims description 100
- 102000039446 nucleic acids Human genes 0.000 claims description 97
- 108020004707 nucleic acids Proteins 0.000 claims description 97
- 230000001419 dependent effect Effects 0.000 claims description 95
- 238000000034 method Methods 0.000 claims description 75
- 102000023888 sequence-specific DNA binding proteins Human genes 0.000 claims description 74
- 108091008420 sequence-specific DNA binding proteins Proteins 0.000 claims description 74
- 229920001184 polypeptide Polymers 0.000 claims description 69
- 102000004196 processed proteins & peptides Human genes 0.000 claims description 69
- 230000027455 binding Effects 0.000 claims description 57
- 102000004169 proteins and genes Human genes 0.000 claims description 57
- 108090000623 proteins and genes Proteins 0.000 claims description 57
- 102000004533 Endonucleases Human genes 0.000 claims description 53
- 108010042407 Endonucleases Proteins 0.000 claims description 53
- 108010008532 Deoxyribonuclease I Proteins 0.000 claims description 48
- 102000007260 Deoxyribonuclease I Human genes 0.000 claims description 48
- 230000000694 effects Effects 0.000 claims description 46
- 239000012636 effector Substances 0.000 claims description 39
- 238000010459 TALEN Methods 0.000 claims description 27
- 108091033319 polynucleotide Proteins 0.000 claims description 25
- 102000040430 polynucleotide Human genes 0.000 claims description 25
- 239000002157 polynucleotide Substances 0.000 claims description 25
- 230000014789 establishment of RNA localization Effects 0.000 claims description 21
- 101710125418 Major capsid protein Proteins 0.000 claims description 19
- 101710132601 Capsid protein Proteins 0.000 claims description 11
- 101710094648 Coat protein Proteins 0.000 claims description 11
- 102100021181 Golgi phosphoprotein 3 Human genes 0.000 claims description 11
- 101710141454 Nucleoprotein Proteins 0.000 claims description 11
- 101710083689 Probable capsid protein Proteins 0.000 claims description 11
- 108060002716 Exonuclease Proteins 0.000 claims description 10
- 102000013165 exonuclease Human genes 0.000 claims description 10
- 108010011170 Ala-Trp-Arg-His-Pro-Gln-Phe-Gly-Gly Proteins 0.000 claims description 9
- 108010063113 DNA Polymerase II Proteins 0.000 claims description 9
- 102000010567 DNA Polymerase II Human genes 0.000 claims description 9
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims description 8
- 108010017842 Telomerase Proteins 0.000 claims description 8
- 230000004568 DNA-binding Effects 0.000 claims description 7
- 101710163270 Nuclease Proteins 0.000 claims description 7
- 108091008324 binding proteins Proteins 0.000 claims description 7
- 101100107610 Arabidopsis thaliana ABCF4 gene Proteins 0.000 claims description 6
- 108091033409 CRISPR Proteins 0.000 claims description 6
- 108700022150 Designed Ankyrin Repeat Proteins Proteins 0.000 claims description 6
- 101710135898 Myc proto-oncogene protein Proteins 0.000 claims description 6
- 102100038895 Myc proto-oncogene protein Human genes 0.000 claims description 6
- 101100068078 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GCN4 gene Proteins 0.000 claims description 6
- 101710150448 Transcriptional regulator Myc Proteins 0.000 claims description 6
- 102000008682 Argonaute Proteins Human genes 0.000 claims description 5
- 108010088141 Argonaute Proteins Proteins 0.000 claims description 5
- 102000052510 DNA-Binding Proteins Human genes 0.000 claims description 5
- 101710096438 DNA-binding protein Proteins 0.000 claims description 5
- 108010092799 RNA-directed DNA polymerase Proteins 0.000 claims description 5
- 230000001580 bacterial effect Effects 0.000 claims description 5
- 101710159080 Aconitate hydratase A Proteins 0.000 claims description 4
- 101710159078 Aconitate hydratase B Proteins 0.000 claims description 4
- 108091023037 Aptamer Proteins 0.000 claims description 4
- 102000053602 DNA Human genes 0.000 claims description 4
- 108010002747 Pfu DNA polymerase Proteins 0.000 claims description 4
- 108091008103 RNA aptamers Proteins 0.000 claims description 4
- 102000044126 RNA-Binding Proteins Human genes 0.000 claims description 4
- 101710105008 RNA-binding protein Proteins 0.000 claims description 4
- 101000844752 Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2) DNA-binding protein 7d Proteins 0.000 claims description 4
- 101150014310 fem-3 gene Proteins 0.000 claims description 4
- 239000003446 ligand Substances 0.000 claims description 4
- 108020004999 messenger RNA Proteins 0.000 claims description 4
- 108010017826 DNA Polymerase I Proteins 0.000 claims description 3
- 102000004594 DNA Polymerase I Human genes 0.000 claims description 3
- 108010071146 DNA Polymerase III Proteins 0.000 claims description 3
- 102000007528 DNA Polymerase III Human genes 0.000 claims description 3
- 241000588724 Escherichia coli Species 0.000 claims description 3
- 230000002452 interceptive effect Effects 0.000 claims description 2
- 102000023732 binding proteins Human genes 0.000 claims 2
- 108010073062 Transcription Activator-Like Effectors Proteins 0.000 claims 1
- 239000002131 composite material Substances 0.000 claims 1
- 230000005782 double-strand break Effects 0.000 claims 1
- 230000001747 exhibiting effect Effects 0.000 claims 1
- 239000012634 fragment Substances 0.000 claims 1
- 108010017070 Zinc Finger Nucleases Proteins 0.000 description 12
- 230000007115 recruitment Effects 0.000 description 8
- 241001673669 Porcine circovirus 2 Species 0.000 description 6
- 102000014914 Carrier Proteins Human genes 0.000 description 5
- -1 Ku-heterodimers) Proteins 0.000 description 3
- 108091028043 Nucleic acid sequence Proteins 0.000 description 3
- 241000205091 Sulfolobus solfataricus Species 0.000 description 3
- 230000003993 interaction Effects 0.000 description 3
- 108091032973 (ribonucleotides)n+m Proteins 0.000 description 2
- 108700004991 Cas12a Proteins 0.000 description 2
- 108091008102 DNA aptamers Proteins 0.000 description 2
- 108091028649 Multicopy single-stranded DNA Proteins 0.000 description 2
- 108020005120 Plant DNA Proteins 0.000 description 2
- 108091030145 Retron msr RNA Proteins 0.000 description 2
- 108010001244 Tli polymerase Proteins 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 238000000638 solvent extraction Methods 0.000 description 2
- 125000006850 spacer group Chemical group 0.000 description 2
- 108010010677 Phosphodiesterase I Proteins 0.000 description 1
- 239000012190 activator Substances 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- 230000033607 mismatch repair Effects 0.000 description 1
- 108010068698 spleen exonuclease Proteins 0.000 description 1
- 238000013518 transcription Methods 0.000 description 1
- 230000035897 transcription Effects 0.000 description 1
- 230000002103 transcriptional effect Effects 0.000 description 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US202062957542P | 2020-01-06 | 2020-01-06 | |
| US62/957,542 | 2020-01-06 | ||
| PCT/US2021/012283 WO2021141970A1 (fr) | 2020-01-06 | 2021-01-06 | Recrutement d'adn polymérase pour édition à matrice |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2023510744A JP2023510744A (ja) | 2023-03-15 |
| JPWO2021141970A5 true JPWO2021141970A5 (fr) | 2024-01-17 |
Family
ID=76654086
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2022541803A Pending JP2023510744A (ja) | 2020-01-06 | 2021-01-06 | 鋳型を用いた編集のためのｄｎａポリメラーゼのリクルート |
Country Status (10)
| Country | Link |
|---|---|
| US (1) | US20210207113A1 (fr) |
| EP (1) | EP4087599A4 (fr) |
| JP (1) | JP2023510744A (fr) |
| KR (1) | KR20220125304A (fr) |
| CN (1) | CN115209915A (fr) |
| AU (1) | AU2021206639A1 (fr) |
| CA (1) | CA3165756A1 (fr) |
| IL (1) | IL294190A (fr) |
| MX (1) | MX2022008359A (fr) |
| WO (1) | WO2021141970A1 (fr) |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA3231678A1 (fr) * | 2021-09-08 | 2023-03-16 | Flagship Pioneering Innovations Vi, Llc | Recrutement en trans dans des composants de systeme d'edition de gene |
| WO2023133415A1 (fr) * | 2022-01-04 | 2023-07-13 | Inscripta, Inc. | Stratégies de recrutement direct de modèles de réparation pour des nucléases crispr |
| US11866728B2 (en) | 2022-01-21 | 2024-01-09 | Renagade Therapeutics Management Inc. | Engineered retrons and methods of use |
| WO2024059811A2 (fr) * | 2022-09-15 | 2024-03-21 | Enceladus Bio, Inc. | Édition de gènes dirigée par un rétron |
Family Cites Families (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102272319B (zh) * | 2009-01-08 | 2014-08-27 | 伯乐实验室公司 | 用于提高核酸扩增反应效率的方法和组合物 |
| WO2012129610A1 (fr) * | 2011-03-31 | 2012-10-04 | Tgr Biosciences Pty Ltd | Détection d'analytes par dosage immunologique en une étape |
| US20150067922A1 (en) * | 2013-05-30 | 2015-03-05 | The Penn State Research Foundation | Gene targeting and genetic modification of plants via rna-guided genome editing |
| US10538758B2 (en) * | 2015-08-19 | 2020-01-21 | Arc Bio, Llc | Capture of nucleic acids using a nucleic acid-guided nuclease-based system |
| WO2017151719A1 (fr) * | 2016-03-01 | 2017-09-08 | University Of Florida Research Foundation, Incorporated | Système d'agenda cellulaire moléculaire |
| WO2019051097A1 (fr) * | 2017-09-08 | 2019-03-14 | The Regents Of The University Of California | Polypeptides de fusion d'endonucléase guidée par arn et procédés d'utilisation correspondants |
| CN110607355A (zh) * | 2019-02-18 | 2019-12-24 | 华东理工大学 | 一种基于Cas9切口酶偶联DNA聚合酶的恒温核酸检测分析方法及试剂盒 |
-
2021
- 2021-01-06 EP EP21738783.6A patent/EP4087599A4/fr active Pending
- 2021-01-06 AU AU2021206639A patent/AU2021206639A1/en active Pending
- 2021-01-06 KR KR1020227027159A patent/KR20220125304A/ko unknown
- 2021-01-06 US US17/142,570 patent/US20210207113A1/en active Pending
- 2021-01-06 MX MX2022008359A patent/MX2022008359A/es unknown
- 2021-01-06 IL IL294190A patent/IL294190A/en unknown
- 2021-01-06 JP JP2022541803A patent/JP2023510744A/ja active Pending
- 2021-01-06 CN CN202180017782.8A patent/CN115209915A/zh active Pending
- 2021-01-06 CA CA3165756A patent/CA3165756A1/fr active Pending
- 2021-01-06 WO PCT/US2021/012283 patent/WO2021141970A1/fr unknown
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