JPWO2021084419A5 - - Google Patents

Download PDF

Info

Publication number
JPWO2021084419A5
JPWO2021084419A5 JP2022551433A JP2022551433A JPWO2021084419A5 JP WO2021084419 A5 JPWO2021084419 A5 JP WO2021084419A5 JP 2022551433 A JP2022551433 A JP 2022551433A JP 2022551433 A JP2022551433 A JP 2022551433A JP WO2021084419 A5 JPWO2021084419 A5 JP WO2021084419A5
Authority
JP
Japan
Prior art keywords
sequence
rca
grid oligonucleotide
grid
products
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP2022551433A
Other languages
English (en)
Japanese (ja)
Other versions
JP2022554421A5 (https=
JP2022554421A (ja
JP7684984B2 (ja
Publication date
Application filed filed Critical
Priority claimed from PCT/IB2020/060062 external-priority patent/WO2021084419A1/en
Publication of JP2022554421A publication Critical patent/JP2022554421A/ja
Publication of JPWO2021084419A5 publication Critical patent/JPWO2021084419A5/ja
Publication of JP2022554421A5 publication Critical patent/JP2022554421A5/ja
Application granted granted Critical
Publication of JP7684984B2 publication Critical patent/JP7684984B2/ja
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

JP2022551433A 2019-10-28 2020-10-27 ローリングサークル増幅産物をマッピングするための方法 Active JP7684984B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201962926907P 2019-10-28 2019-10-28
US62/926,907 2019-10-28
PCT/IB2020/060062 WO2021084419A1 (en) 2019-10-28 2020-10-27 Method for mapping rolling circle amplification products

Publications (4)

Publication Number Publication Date
JP2022554421A JP2022554421A (ja) 2022-12-28
JPWO2021084419A5 true JPWO2021084419A5 (https=) 2023-10-30
JP2022554421A5 JP2022554421A5 (https=) 2023-10-30
JP7684984B2 JP7684984B2 (ja) 2025-05-28

Family

ID=73172763

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2022551433A Active JP7684984B2 (ja) 2019-10-28 2020-10-27 ローリングサークル増幅産物をマッピングするための方法

Country Status (8)

Country Link
US (3) US12252741B2 (https=)
EP (1) EP4051810B1 (https=)
JP (1) JP7684984B2 (https=)
CN (1) CN114616344B (https=)
AU (1) AU2020375212B2 (https=)
CA (1) CA3157211A1 (https=)
DK (1) DK4051810T3 (https=)
WO (1) WO2021084419A1 (https=)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2022032195A2 (en) 2020-08-06 2022-02-10 Singular Genomics Systems, Inc. Spatial sequencing
EP4121561A4 (en) 2020-08-06 2024-04-17 Singular Genomics Systems, Inc. Methods for in situ transcriptomics and proteomics
EP4303315B1 (en) 2021-03-30 2024-11-06 Pixelgen Technologies Ab Spatial mapping by serial primer extension
EP4720332A1 (en) 2023-06-01 2026-04-08 Singular Genomics Systems, Inc. Methods and probes for detecting polynucleotide sequences in cells and tissues
WO2025158200A1 (en) 2024-01-25 2025-07-31 Pixelgen Technologies Ab Method for spatial mapping of cell targets

Family Cites Families (25)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5948902A (en) 1997-11-20 1999-09-07 South Alabama Medical Science Foundation Antisense oligonucleotides to human serine/threonine protein phosphatase genes
US20050118616A1 (en) * 2002-08-16 2005-06-02 Kawashima Tadashi R. Amplification of target nucleotide sequence without polymerase chain reaction
AU2004232976B2 (en) * 2003-04-18 2011-02-10 Becton, Dickinson And Company Immuno-amplification
US20050233340A1 (en) 2004-04-20 2005-10-20 Barrett Michael T Methods and compositions for assessing CpG methylation
US8916351B2 (en) * 2005-01-04 2014-12-23 Hitachi Chemical Co., Ltd. Primer generation rolling circle amplification
US8481698B2 (en) 2009-03-19 2013-07-09 The President And Fellows Of Harvard College Parallel proximity ligation event analysis
EP2633081B1 (en) * 2010-10-29 2017-01-11 Olink Bioscience AB Proximity ligation technology for western blot applications
JP6069224B2 (ja) 2011-01-31 2017-02-01 アプライズ バイオ, インコーポレイテッド 細胞において複数のエピトープを同定する方法
EP3736281A1 (en) 2011-02-18 2020-11-11 Bio-Rad Laboratories, Inc. Compositions and methods for molecular labeling
GB201201547D0 (en) 2012-01-30 2012-03-14 Olink Ab Method and product
WO2014076209A1 (en) * 2012-11-14 2014-05-22 Olink Ab Localised rca-based amplification method
CN105745334B (zh) * 2013-09-30 2019-12-10 吴迪 通过使用邻近条形编码分析分子复合物的概况的方法
EP3058091B1 (en) 2013-10-18 2020-03-25 The Broad Institute, Inc. Spatial and cellular mapping of biomolecules in situ by high-throughput sequencing
GB201320145D0 (en) * 2013-11-14 2014-01-01 Olink Ab Localised RCA-based amplification method using a padlock-probe
US20150298091A1 (en) 2014-04-21 2015-10-22 President And Fellows Of Harvard College Systems and methods for barcoding nucleic acids
US9909167B2 (en) * 2014-06-23 2018-03-06 The Board Of Trustees Of The Leland Stanford Junior University On-slide staining by primer extension
ES2891085T3 (es) 2015-01-30 2022-01-26 Harvard College Obtención de imágenes sin microscopio
WO2017044893A1 (en) 2015-09-11 2017-03-16 The Broad Institute, Inc. Dna microscopy
US11092607B2 (en) * 2015-10-28 2021-08-17 The Board Institute, Inc. Multiplex analysis of single cell constituents
EP4050112A1 (en) 2016-06-21 2022-08-31 10X Genomics, Inc. Nucleic acid sequencing
EP3583214B1 (en) * 2017-02-02 2023-11-22 New York Genome Center, Inc. Methods and compositions for identifying or quantifying targets in a biological sample
GB201718681D0 (en) * 2017-11-13 2017-12-27 Evox Therapeutics Ltd Protein engineered extracellular vesicles
EP3954782A1 (en) 2017-11-15 2022-02-16 10X Genomics, Inc. Functionalized gel beads
EP4477758B1 (en) 2018-11-30 2026-04-22 Illumina, Inc. Analysis of multiple analytes using a single assay
CN114787348B (zh) 2019-09-30 2025-09-19 耶鲁大学 用于空间组学测序的确定性条形码

Similar Documents

Publication Publication Date Title
JP6925424B2 (ja) 短いdna断片を連結することによる一分子シーケンスのスループットを増加する方法
US8986958B2 (en) Methods for generating target specific probes for solution based capture
CN107208144B (zh) 无酶且无扩增的测序
EP2191011B1 (en) Method for sequencing a polynucleotide template
US20050112636A1 (en) Polymeric nucleic acid hybridization probes
US20030082576A1 (en) High throughput polymorphism screening
US20100092972A1 (en) Assay for gene expression
JP2018503403A (ja) 液相におけるライゲーションアッセイ
US10174352B2 (en) Methods for amplification of nucleic acids on solid support
KR20160138579A (ko) 게놈 및 치료학적 적용을 위한 핵산 분자의 클론 복제 및 증폭을 위한 시스템 및 방법
JP2013524841A (ja) 一本鎖オーバーハングを有する二本鎖核酸を生成するための方法
JPWO2021084419A5 (https=)
CA3220708A1 (en) Oligo-modified nucleotide analogues for nucleic acid preparation
CN107109478B (zh) 获得配对末端测序信息的方法
JP2006508677A (ja) 遺伝子発現のオリゴヌクレオチド誘導分析
Myllykangas et al. Targeted sequencing library preparation by genomic DNA circularization
WO2000029624A2 (en) Methods and compositions for detection of nucleic acid sequences and signal amplification
WO2023116373A1 (zh) 一种生成标记的核酸分子群的方法及其试剂盒
US20250369045A1 (en) Multivalent assemblies for enhanced target hybridization
WO2023115536A1 (zh) 一种生成标记的核酸分子群的方法及其试剂盒
JP7610617B2 (ja) 反応混合物からの過剰なオリゴヌクレオチドの除去
EP4431450A1 (en) Supporter for sequencing target nucleic acid strands
RU2809771C2 (ru) Композиции и способы для улучшения обогащения библиотек
WO2023116376A1 (zh) 单细胞核酸标记和分析方法
JP2024506277A (ja) 脱塩基核酸を有する配列変換及びシグナル増幅dna、並びにそれを使用する検出方法