CA3157211A1 - Method for mapping rolling circle amplification products - Google Patents
Method for mapping rolling circle amplification productsInfo
- Publication number
- CA3157211A1 CA3157211A1 CA3157211A CA3157211A CA3157211A1 CA 3157211 A1 CA3157211 A1 CA 3157211A1 CA 3157211 A CA3157211 A CA 3157211A CA 3157211 A CA3157211 A CA 3157211A CA 3157211 A1 CA3157211 A1 CA 3157211A1
- Authority
- CA
- Canada
- Prior art keywords
- rca
- grid
- sequence
- products
- rca products
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6804—Nucleic acid analysis using immunogens
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6841—In situ hybridisation
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
- C12Q2525/10—Modifications characterised by
- C12Q2525/179—Modifications characterised by incorporating arbitrary or random nucleotide sequences
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2531/00—Reactions of nucleic acids characterised by
- C12Q2531/10—Reactions of nucleic acids characterised by the purpose being amplify/increase the copy number of target nucleic acid
- C12Q2531/125—Rolling circle
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
- C12Q2563/179—Nucleic acid detection characterized by the use of physical, structural and functional properties the label being a nucleic acid
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
- C12Q2565/50—Detection characterised by immobilisation to a surface
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
- C12Q2565/50—Detection characterised by immobilisation to a surface
- C12Q2565/537—Detection characterised by immobilisation to a surface characterised by the capture oligonucleotide acting as a primer
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/16—Primer sets for multiplex assays
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Biophysics (AREA)
- General Engineering & Computer Science (AREA)
- Immunology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Genetics & Genomics (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201962926907P | 2019-10-28 | 2019-10-28 | |
| US62/926,907 | 2019-10-28 | ||
| PCT/IB2020/060062 WO2021084419A1 (en) | 2019-10-28 | 2020-10-27 | Method for mapping rolling circle amplification products |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA3157211A1 true CA3157211A1 (en) | 2021-05-06 |
Family
ID=73172763
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA3157211A Pending CA3157211A1 (en) | 2019-10-28 | 2020-10-27 | Method for mapping rolling circle amplification products |
Country Status (8)
| Country | Link |
|---|---|
| US (3) | US12252741B2 (https=) |
| EP (1) | EP4051810B1 (https=) |
| JP (1) | JP7684984B2 (https=) |
| CN (1) | CN114616344B (https=) |
| AU (1) | AU2020375212B2 (https=) |
| CA (1) | CA3157211A1 (https=) |
| DK (1) | DK4051810T3 (https=) |
| WO (1) | WO2021084419A1 (https=) |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2022032195A2 (en) | 2020-08-06 | 2022-02-10 | Singular Genomics Systems, Inc. | Spatial sequencing |
| EP4121561A4 (en) | 2020-08-06 | 2024-04-17 | Singular Genomics Systems, Inc. | Methods for in situ transcriptomics and proteomics |
| EP4303315B1 (en) | 2021-03-30 | 2024-11-06 | Pixelgen Technologies Ab | Spatial mapping by serial primer extension |
| EP4720332A1 (en) | 2023-06-01 | 2026-04-08 | Singular Genomics Systems, Inc. | Methods and probes for detecting polynucleotide sequences in cells and tissues |
| WO2025158200A1 (en) | 2024-01-25 | 2025-07-31 | Pixelgen Technologies Ab | Method for spatial mapping of cell targets |
Family Cites Families (25)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5948902A (en) | 1997-11-20 | 1999-09-07 | South Alabama Medical Science Foundation | Antisense oligonucleotides to human serine/threonine protein phosphatase genes |
| US20050118616A1 (en) * | 2002-08-16 | 2005-06-02 | Kawashima Tadashi R. | Amplification of target nucleotide sequence without polymerase chain reaction |
| AU2004232976B2 (en) * | 2003-04-18 | 2011-02-10 | Becton, Dickinson And Company | Immuno-amplification |
| US20050233340A1 (en) | 2004-04-20 | 2005-10-20 | Barrett Michael T | Methods and compositions for assessing CpG methylation |
| US8916351B2 (en) * | 2005-01-04 | 2014-12-23 | Hitachi Chemical Co., Ltd. | Primer generation rolling circle amplification |
| US8481698B2 (en) | 2009-03-19 | 2013-07-09 | The President And Fellows Of Harvard College | Parallel proximity ligation event analysis |
| EP2633081B1 (en) * | 2010-10-29 | 2017-01-11 | Olink Bioscience AB | Proximity ligation technology for western blot applications |
| JP6069224B2 (ja) | 2011-01-31 | 2017-02-01 | アプライズ バイオ， インコーポレイテッド | 細胞において複数のエピトープを同定する方法 |
| EP3736281A1 (en) | 2011-02-18 | 2020-11-11 | Bio-Rad Laboratories, Inc. | Compositions and methods for molecular labeling |
| GB201201547D0 (en) | 2012-01-30 | 2012-03-14 | Olink Ab | Method and product |
| WO2014076209A1 (en) * | 2012-11-14 | 2014-05-22 | Olink Ab | Localised rca-based amplification method |
| CN105745334B (zh) * | 2013-09-30 | 2019-12-10 | 吴迪 | 通过使用邻近条形编码分析分子复合物的概况的方法 |
| EP3058091B1 (en) | 2013-10-18 | 2020-03-25 | The Broad Institute, Inc. | Spatial and cellular mapping of biomolecules in situ by high-throughput sequencing |
| GB201320145D0 (en) * | 2013-11-14 | 2014-01-01 | Olink Ab | Localised RCA-based amplification method using a padlock-probe |
| US20150298091A1 (en) | 2014-04-21 | 2015-10-22 | President And Fellows Of Harvard College | Systems and methods for barcoding nucleic acids |
| US9909167B2 (en) * | 2014-06-23 | 2018-03-06 | The Board Of Trustees Of The Leland Stanford Junior University | On-slide staining by primer extension |
| ES2891085T3 (es) | 2015-01-30 | 2022-01-26 | Harvard College | Obtención de imágenes sin microscopio |
| WO2017044893A1 (en) | 2015-09-11 | 2017-03-16 | The Broad Institute, Inc. | Dna microscopy |
| US11092607B2 (en) * | 2015-10-28 | 2021-08-17 | The Board Institute, Inc. | Multiplex analysis of single cell constituents |
| EP4050112A1 (en) | 2016-06-21 | 2022-08-31 | 10X Genomics, Inc. | Nucleic acid sequencing |
| EP3583214B1 (en) * | 2017-02-02 | 2023-11-22 | New York Genome Center, Inc. | Methods and compositions for identifying or quantifying targets in a biological sample |
| GB201718681D0 (en) * | 2017-11-13 | 2017-12-27 | Evox Therapeutics Ltd | Protein engineered extracellular vesicles |
| EP3954782A1 (en) | 2017-11-15 | 2022-02-16 | 10X Genomics, Inc. | Functionalized gel beads |
| EP4477758B1 (en) | 2018-11-30 | 2026-04-22 | Illumina, Inc. | Analysis of multiple analytes using a single assay |
| CN114787348B (zh) | 2019-09-30 | 2025-09-19 | 耶鲁大学 | 用于空间组学测序的确定性条形码 |
-
2020
- 2020-10-27 US US17/763,145 patent/US12252741B2/en active Active
- 2020-10-27 EP EP20803646.7A patent/EP4051810B1/en active Active
- 2020-10-27 AU AU2020375212A patent/AU2020375212B2/en active Active
- 2020-10-27 CN CN202080075923.7A patent/CN114616344B/zh active Active
- 2020-10-27 WO PCT/IB2020/060062 patent/WO2021084419A1/en not_active Ceased
- 2020-10-27 CA CA3157211A patent/CA3157211A1/en active Pending
- 2020-10-27 JP JP2022551433A patent/JP7684984B2/ja active Active
- 2020-10-27 DK DK20803646.7T patent/DK4051810T3/da active
-
2025
- 2025-02-10 US US19/049,977 patent/US20250305038A1/en active Pending
- 2025-05-19 US US19/212,481 patent/US12404545B1/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| EP4051810B1 (en) | 2024-08-28 |
| US12252741B2 (en) | 2025-03-18 |
| CN114616344B (zh) | 2026-03-31 |
| AU2020375212A1 (en) | 2022-04-28 |
| JP2022554421A (ja) | 2022-12-28 |
| US20250305038A1 (en) | 2025-10-02 |
| EP4051810A1 (en) | 2022-09-07 |
| DK4051810T3 (da) | 2024-09-09 |
| US12404545B1 (en) | 2025-09-02 |
| JP7684984B2 (ja) | 2025-05-28 |
| WO2021084419A1 (en) | 2021-05-06 |
| US20250277259A1 (en) | 2025-09-04 |
| AU2020375212B2 (en) | 2026-02-05 |
| CN114616344A (zh) | 2022-06-10 |
| US20230027467A1 (en) | 2023-01-26 |
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