JP7684984B2 - ローリングサークル増幅産物をマッピングするための方法 - Google Patents

ローリングサークル増幅産物をマッピングするための方法 Download PDF

Info

Publication number
JP7684984B2
JP7684984B2 JP2022551433A JP2022551433A JP7684984B2 JP 7684984 B2 JP7684984 B2 JP 7684984B2 JP 2022551433 A JP2022551433 A JP 2022551433A JP 2022551433 A JP2022551433 A JP 2022551433A JP 7684984 B2 JP7684984 B2 JP 7684984B2
Authority
JP
Japan
Prior art keywords
rca
grid
sequence
products
grid oligonucleotide
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
JP2022551433A
Other languages
English (en)
Japanese (ja)
Other versions
JP2022554421A5 (https=
JP2022554421A (ja
Inventor
フレドリクソン，サイモン
Original Assignee
ピクセルゲン テクノロジーズ エービー
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by ピクセルゲン テクノロジーズ エービー filed Critical ピクセルゲン テクノロジーズ エービー
Publication of JP2022554421A publication Critical patent/JP2022554421A/ja
Publication of JP2022554421A5 publication Critical patent/JP2022554421A5/ja
Application granted granted Critical
Publication of JP7684984B2 publication Critical patent/JP7684984B2/ja
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6804—Nucleic acid analysis using immunogens
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6841—In situ hybridisation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/179—Modifications characterised by incorporating arbitrary or random nucleotide sequences
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2531/00—Reactions of nucleic acids characterised by
    • C12Q2531/10—Reactions of nucleic acids characterised by the purpose being amplify/increase the copy number of target nucleic acid
    • C12Q2531/125—Rolling circle
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/179—Nucleic acid detection characterized by the use of physical, structural and functional properties the label being a nucleic acid
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
    • C12Q2565/50—Detection characterised by immobilisation to a surface
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
    • C12Q2565/50—Detection characterised by immobilisation to a surface
    • C12Q2565/537—Detection characterised by immobilisation to a surface characterised by the capture oligonucleotide acting as a primer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/16—Primer sets for multiplex assays

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • General Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
JP2022551433A 2019-10-28 2020-10-27 ローリングサークル増幅産物をマッピングするための方法 Active JP7684984B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201962926907P 2019-10-28 2019-10-28
US62/926,907 2019-10-28
PCT/IB2020/060062 WO2021084419A1 (en) 2019-10-28 2020-10-27 Method for mapping rolling circle amplification products

Publications (3)

Publication Number Publication Date
JP2022554421A JP2022554421A (ja) 2022-12-28
JP2022554421A5 JP2022554421A5 (https=) 2023-10-30
JP7684984B2 true JP7684984B2 (ja) 2025-05-28

Family

ID=73172763

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2022551433A Active JP7684984B2 (ja) 2019-10-28 2020-10-27 ローリングサークル増幅産物をマッピングするための方法

Country Status (8)

Country Link
US (3) US12252741B2 (https=)
EP (1) EP4051810B1 (https=)
JP (1) JP7684984B2 (https=)
CN (1) CN114616344B (https=)
AU (1) AU2020375212B2 (https=)
CA (1) CA3157211A1 (https=)
DK (1) DK4051810T3 (https=)
WO (1) WO2021084419A1 (https=)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11492662B2 (en) 2020-08-06 2022-11-08 Singular Genomics Systems, Inc. Methods for in situ transcriptomics and proteomics
US11434525B2 (en) 2020-08-06 2022-09-06 Singular Genomics Systems, Inc. Spatial sequencing
EP4303315B1 (en) * 2021-03-30 2024-11-06 Pixelgen Technologies Ab Spatial mapping by serial primer extension
EP4720332A1 (en) 2023-06-01 2026-04-08 Singular Genomics Systems, Inc. Methods and probes for detecting polynucleotide sequences in cells and tissues
WO2025158200A1 (en) 2024-01-25 2025-07-31 Pixelgen Technologies Ab Method for spatial mapping of cell targets

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2007525174A (ja) 2003-04-18 2007-09-06 ベクトン・ディキンソン・アンド・カンパニー 免疫増幅
WO2012057689A1 (en) 2010-10-29 2012-05-03 Ge Healthcare Bio-Sciences Ab Proximity ligation technology for western blot applications

Family Cites Families (23)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5948902A (en) 1997-11-20 1999-09-07 South Alabama Medical Science Foundation Antisense oligonucleotides to human serine/threonine protein phosphatase genes
US20050118616A1 (en) * 2002-08-16 2005-06-02 Kawashima Tadashi R. Amplification of target nucleotide sequence without polymerase chain reaction
US20050233340A1 (en) 2004-04-20 2005-10-20 Barrett Michael T Methods and compositions for assessing CpG methylation
EP1833994A4 (en) * 2005-01-04 2009-09-02 Hitachi Chemical Co Ltd ROLLING CIRCLE GAINING OF A PRIMER GENERATION
US8481698B2 (en) 2009-03-19 2013-07-09 The President And Fellows Of Harvard College Parallel proximity ligation event analysis
WO2012106385A2 (en) 2011-01-31 2012-08-09 Apprise Bio, Inc. Methods of identifying multiple epitopes in cells
WO2012112804A1 (en) 2011-02-18 2012-08-23 Raindance Technoligies, Inc. Compositions and methods for molecular labeling
GB201201547D0 (en) 2012-01-30 2012-03-14 Olink Ab Method and product
EP2920324B1 (en) * 2012-11-14 2017-12-27 Olink Bioscience AB Localised rca-based amplification method
CN105745334B (zh) 2013-09-30 2019-12-10 吴迪 通过使用邻近条形编码分析分子复合物的概况的方法
EP3058091B1 (en) 2013-10-18 2020-03-25 The Broad Institute, Inc. Spatial and cellular mapping of biomolecules in situ by high-throughput sequencing
GB201320145D0 (en) * 2013-11-14 2014-01-01 Olink Ab Localised RCA-based amplification method using a padlock-probe
US20150298091A1 (en) 2014-04-21 2015-10-22 President And Fellows Of Harvard College Systems and methods for barcoding nucleic acids
US9909167B2 (en) * 2014-06-23 2018-03-06 The Board Of Trustees Of The Leland Stanford Junior University On-slide staining by primer extension
US11639522B2 (en) 2015-01-30 2023-05-02 President And Fellows Of Harvard College Microscope-free imaging
WO2017044893A1 (en) 2015-09-11 2017-03-16 The Broad Institute, Inc. Dna microscopy
US11092607B2 (en) * 2015-10-28 2021-08-17 The Board Institute, Inc. Multiplex analysis of single cell constituents
US11352667B2 (en) * 2016-06-21 2022-06-07 10X Genomics, Inc. Nucleic acid sequencing
CN117887804A (zh) * 2017-02-02 2024-04-16 纽约基因组研究中心公司 用于识别或量化在生物样品中的靶标的方法和组合物
GB201718681D0 (en) * 2017-11-13 2017-12-27 Evox Therapeutics Ltd Protein engineered extracellular vesicles
SG11201913654QA (en) 2017-11-15 2020-01-30 10X Genomics Inc Functionalized gel beads
CN112867801B (zh) 2018-11-30 2024-07-12 Illumina公司 使用单一测定分析多种分析物
AU2020357801A1 (en) 2019-09-30 2022-04-14 Yale University Deterministic Barcoding for spatial omics sequencing

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2007525174A (ja) 2003-04-18 2007-09-06 ベクトン・ディキンソン・アンド・カンパニー 免疫増幅
WO2012057689A1 (en) 2010-10-29 2012-05-03 Ge Healthcare Bio-Sciences Ab Proximity ligation technology for western blot applications

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
GREENWOOD, Christina et al.，"Proximity assays for sensitive quantification of proteins"，Biomolecular Detection and Quantification，2015年，Vol. 4，pp. 10-16，DOI:10.1016/j.bdq.2015.04.002
GUSTAFSDOTTIR, Sigrun M. et al.，"Proximity ligation assays for sensitive and specific protein analyses"，Analytical Biochemistry，2005年，Vol. 345，pp. 2-9，DOI:10.1016/j.ab.2005.01.018
WU, Di et al.，"Profiling surface proteins on individual exosomes using a proximity barcoding assay"，Nature Communications，2019年08月26日，Vol. 10, Article 3854，DOI:10.1038/s41467-019-11486-1

Also Published As

Publication number Publication date
EP4051810B1 (en) 2024-08-28
CA3157211A1 (en) 2021-05-06
US20250305038A1 (en) 2025-10-02
US12252741B2 (en) 2025-03-18
CN114616344B (zh) 2026-03-31
CN114616344A (zh) 2022-06-10
DK4051810T3 (da) 2024-09-09
AU2020375212B2 (en) 2026-02-05
US20230027467A1 (en) 2023-01-26
US12404545B1 (en) 2025-09-02
JP2022554421A (ja) 2022-12-28
AU2020375212A1 (en) 2022-04-28
US20250277259A1 (en) 2025-09-04
WO2021084419A1 (en) 2021-05-06
EP4051810A1 (en) 2022-09-07

Similar Documents

Publication Publication Date Title
JP7684984B2 (ja) ローリングサークル増幅産物をマッピングするための方法
EP2920320B1 (en) Rca reporter probes and their use in detecting nucleic acid molecules
US20240336959A1 (en) Method for making a physical map of a population of barcoded particles
US20080293051A1 (en) proximity ligation assay
EP2589657A1 (en) Method for detection of target molecule
CN106460065A (zh) 用于基因组应用和治疗应用的核酸分子的克隆复制和扩增的系统和方法
US12416040B2 (en) Spatial mapping by serial primer extension
Boone et al. Capturing the ‘ome’: the expanding molecular toolbox for RNA and DNA library construction
WO2017096394A1 (en) High-efficiency hybrid capture compositions, and methods
US20250171828A1 (en) Rolling circle amplification product having multiple free ends
CN116635536A (zh) 用于制作条形码化颗粒群的物理图谱的方法
US9828599B2 (en) Methods of selecting binding-elements and uses thereof
JP2023535436A (ja) 多重溶液中タンパク質アレイを用いてスクリーニングした検定試料の多重配列決定用二重バーコードインデックス

Legal Events

Date Code Title Description
A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20231020

A621 Written request for application examination

Free format text: JAPANESE INTERMEDIATE CODE: A621

Effective date: 20231020

A977 Report on retrieval

Free format text: JAPANESE INTERMEDIATE CODE: A971007

Effective date: 20241025

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20241105

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20250205

TRDD Decision of grant or rejection written
A01 Written decision to grant a patent or to grant a registration (utility model)

Free format text: JAPANESE INTERMEDIATE CODE: A01

Effective date: 20250430

A61 First payment of annual fees (during grant procedure)

Free format text: JAPANESE INTERMEDIATE CODE: A61

Effective date: 20250516

R150 Certificate of patent or registration of utility model

Ref document number: 7684984

Country of ref document: JP

Free format text: JAPANESE INTERMEDIATE CODE: R150