JPWO2021065849A1 - - Google Patents

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Publication number
JPWO2021065849A1
JPWO2021065849A1 JP2021551279A JP2021551279A JPWO2021065849A1 JP WO2021065849 A1 JPWO2021065849 A1 JP WO2021065849A1 JP 2021551279 A JP2021551279 A JP 2021551279A JP 2021551279 A JP2021551279 A JP 2021551279A JP WO2021065849 A1 JPWO2021065849 A1 JP WO2021065849A1
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JP
Japan
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Application number
JP2021551279A
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JP7354270B2 (ja
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Publication of JPWO2021065849A1 publication Critical patent/JPWO2021065849A1/ja
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Publication of JP7354270B2 publication Critical patent/JP7354270B2/ja
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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/067—Hepatocytes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/38—Vitamins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/90—Serum-free medium, which may still contain naturally-sourced components
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/11—Epidermal growth factor [EGF]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/70—Enzymes
    • C12N2501/72—Transferases (EC 2.)
    • C12N2501/727—Kinases (EC 2.7.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/45—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from artificially induced pluripotent stem cells

Landscapes

  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biomedical Technology (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
JP2021551279A 2019-09-30 2020-09-29 肝細胞を培養するための培地、肝細胞の製造方法および肝細胞 Active JP7354270B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP2019178462 2019-09-30
JP2019178462 2019-09-30
PCT/JP2020/036766 WO2021065849A1 (ja) 2019-09-30 2020-09-29 肝細胞を培養するための培地、肝細胞の製造方法および肝細胞

Publications (2)

Publication Number Publication Date
JPWO2021065849A1 true JPWO2021065849A1 (ja) 2021-04-08
JP7354270B2 JP7354270B2 (ja) 2023-10-02

Family

ID=75336962

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2021551279A Active JP7354270B2 (ja) 2019-09-30 2020-09-29 肝細胞を培養するための培地、肝細胞の製造方法および肝細胞

Country Status (4)

Country Link
US (1) US20220228112A1 (ja)
EP (1) EP4039795A4 (ja)
JP (1) JP7354270B2 (ja)
WO (1) WO2021065849A1 (ja)

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH01157377A (ja) * 1987-09-09 1989-06-20 Green Cross Corp:The 無血清培地
JP2000245448A (ja) * 1999-03-03 2000-09-12 Sumitomo Bakelite Co Ltd 肝細胞培養方法
CN108823168A (zh) * 2018-07-20 2018-11-16 王星 培养基及其用于建立hbv感染的人源原代肝癌细胞系的方法

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP6025715B2 (ja) * 2010-06-11 2016-11-16 セレクティス アーベー 多能性幹細胞の肝細胞への分化を向上する三次元スキャホールド
JPWO2013183571A1 (ja) * 2012-06-08 2016-01-28 公立大学法人名古屋市立大学 人工多能性幹細胞を肝細胞へ分化誘導する方法
JP2019134682A (ja) 2016-06-01 2019-08-15 国立研究開発法人医薬基盤・健康・栄養研究所 肝幹細胞様細胞の調製方法
CN110923192B (zh) * 2019-11-27 2022-03-08 中国人民解放军第二军医大学 一种成熟肝细胞的长期体外培养方法

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH01157377A (ja) * 1987-09-09 1989-06-20 Green Cross Corp:The 無血清培地
JP2000245448A (ja) * 1999-03-03 2000-09-12 Sumitomo Bakelite Co Ltd 肝細胞培養方法
CN108823168A (zh) * 2018-07-20 2018-11-16 王星 培养基及其用于建立hbv感染的人源原代肝癌细胞系的方法

Also Published As

Publication number Publication date
EP4039795A1 (en) 2022-08-10
EP4039795A4 (en) 2022-12-14
JP7354270B2 (ja) 2023-10-02
WO2021065849A1 (ja) 2021-04-08
US20220228112A1 (en) 2022-07-21

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