JPWO2020236992A5 - - Google Patents
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- Publication number
- JPWO2020236992A5 JPWO2020236992A5 JP2021569060A JP2021569060A JPWO2020236992A5 JP WO2020236992 A5 JPWO2020236992 A5 JP WO2020236992A5 JP 2021569060 A JP2021569060 A JP 2021569060A JP 2021569060 A JP2021569060 A JP 2021569060A JP WO2020236992 A5 JPWO2020236992 A5 JP WO2020236992A5
- Authority
- JP
- Japan
- Prior art keywords
- nucleic acid
- acid construct
- item
- polymerase
- reaction
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
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- 150000007523 nucleic acids Chemical class 0.000 claims description 318
- 108020004707 nucleic acids Proteins 0.000 claims description 308
- 102000039446 nucleic acids Human genes 0.000 claims description 308
- 238000000034 method Methods 0.000 claims description 109
- 238000006243 chemical reaction Methods 0.000 claims description 73
- 239000011541 reaction mixture Substances 0.000 claims description 50
- 108091093088 Amplicon Proteins 0.000 claims description 34
- 239000002773 nucleotide Substances 0.000 claims description 32
- 125000003729 nucleotide group Chemical group 0.000 claims description 32
- 238000003752 polymerase chain reaction Methods 0.000 claims description 25
- 238000009396 hybridization Methods 0.000 claims description 22
- 230000001678 irradiating effect Effects 0.000 claims description 22
- 230000000295 complement effect Effects 0.000 claims description 16
- 238000005842 biochemical reaction Methods 0.000 claims description 15
- 230000003287 optical effect Effects 0.000 claims description 14
- 108060002716 Exonuclease Proteins 0.000 claims description 10
- 108020004711 Nucleic Acid Probes Proteins 0.000 claims description 10
- 230000029087 digestion Effects 0.000 claims description 10
- 102000013165 exonuclease Human genes 0.000 claims description 10
- 239000002853 nucleic acid probe Substances 0.000 claims description 10
- 238000003757 reverse transcription PCR Methods 0.000 claims description 10
- HMFHBZSHGGEWLO-SOOFDHNKSA-N D-ribofuranose Chemical compound OC[C@H]1OC(O)[C@H](O)[C@@H]1O HMFHBZSHGGEWLO-SOOFDHNKSA-N 0.000 claims description 7
- PYMYPHUHKUWMLA-LMVFSUKVSA-N Ribose Natural products OC[C@@H](O)[C@@H](O)[C@@H](O)C=O PYMYPHUHKUWMLA-LMVFSUKVSA-N 0.000 claims description 7
- HMFHBZSHGGEWLO-UHFFFAOYSA-N alpha-D-Furanose-Ribose Natural products OCC1OC(O)C(O)C1O HMFHBZSHGGEWLO-UHFFFAOYSA-N 0.000 claims description 7
- 108010008286 DNA nucleotidylexotransferase Proteins 0.000 claims description 5
- 102100029764 DNA-directed DNA/RNA polymerase mu Human genes 0.000 claims description 5
- 102100031780 Endonuclease Human genes 0.000 claims description 5
- 108010042407 Endonucleases Proteins 0.000 claims description 3
- 239000000523 sample Substances 0.000 description 55
- 239000012530 fluid Substances 0.000 description 24
- 238000003753 real-time PCR Methods 0.000 description 21
- 230000003213 activating effect Effects 0.000 description 20
- 108091028043 Nucleic acid sequence Proteins 0.000 description 16
- 230000003321 amplification Effects 0.000 description 12
- 238000003199 nucleic acid amplification method Methods 0.000 description 12
- 239000007787 solid Substances 0.000 description 11
- 239000003153 chemical reaction reagent Substances 0.000 description 10
- 102000004190 Enzymes Human genes 0.000 description 9
- 108090000790 Enzymes Proteins 0.000 description 9
- 239000000758 substrate Substances 0.000 description 9
- 108700028369 Alleles Proteins 0.000 description 8
- 238000012408 PCR amplification Methods 0.000 description 8
- 239000000126 substance Substances 0.000 description 8
- 238000002493 microarray Methods 0.000 description 5
- 108091034117 Oligonucleotide Proteins 0.000 description 4
- 238000011529 RT qPCR Methods 0.000 description 4
- 239000012491 analyte Substances 0.000 description 3
- 238000003776 cleavage reaction Methods 0.000 description 3
- 238000005259 measurement Methods 0.000 description 3
- 238000010791 quenching Methods 0.000 description 3
- 230000007017 scission Effects 0.000 description 3
- 108010076804 DNA Restriction Enzymes Proteins 0.000 description 2
- 102000003960 Ligases Human genes 0.000 description 2
- 108090000364 Ligases Proteins 0.000 description 2
- 108010092799 RNA-directed DNA polymerase Proteins 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- 230000003993 interaction Effects 0.000 description 2
- 239000011159 matrix material Substances 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000003672 processing method Methods 0.000 description 2
- 230000000171 quenching effect Effects 0.000 description 2
- 238000007476 Maximum Likelihood Methods 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 238000004364 calculation method Methods 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 230000001419 dependent effect Effects 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 230000005284 excitation Effects 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 230000004907 flux Effects 0.000 description 1
- 230000014509 gene expression Effects 0.000 description 1
- 230000000415 inactivating effect Effects 0.000 description 1
- 230000000977 initiatory effect Effects 0.000 description 1
- 230000002934 lysing effect Effects 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000002052 molecular layer Substances 0.000 description 1
- 238000005457 optimization Methods 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 238000013518 transcription Methods 0.000 description 1
- 230000035897 transcription Effects 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
- 230000007704 transition Effects 0.000 description 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201962850239P | 2019-05-20 | 2019-05-20 | |
| US62/850,239 | 2019-05-20 | ||
| PCT/US2020/033884 WO2020236992A1 (en) | 2019-05-20 | 2020-05-20 | Photo-triggered nucleic acid constructs and methods for molecular detection |
Publications (4)
| Publication Number | Publication Date |
|---|---|
| JP2022533228A JP2022533228A (ja) | 2022-07-21 |
| JP2022533228A5 JP2022533228A5 (https=) | 2023-05-29 |
| JPWO2020236992A5 true JPWO2020236992A5 (https=) | 2023-05-29 |
| JP7611859B2 JP7611859B2 (ja) | 2025-01-10 |
Family
ID=73458234
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2021569060A Active JP7611859B2 (ja) | 2019-05-20 | 2020-05-20 | 光によりトリガーされる核酸構築物および分子検出のための方法 |
Country Status (5)
| Country | Link |
|---|---|
| US (1) | US20220282320A1 (https=) |
| EP (1) | EP3973289A4 (https=) |
| JP (1) | JP7611859B2 (https=) |
| CN (1) | CN114467027B (https=) |
| WO (1) | WO2020236992A1 (https=) |
Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP7724632B2 (ja) * | 2020-04-23 | 2025-08-18 | 東ソー株式会社 | 標的核酸の光制御増幅試薬、光制御増幅方法および検出方法 |
| WO2022147139A1 (en) * | 2020-12-30 | 2022-07-07 | 10X Genomics, Inc. | Methods and compositions for light-controlled surface patterning using a polymer |
| EP4271509A1 (en) * | 2020-12-30 | 2023-11-08 | 10X Genomics, Inc. | Molecular arrays and methods for generating and using the arrays |
| WO2024196728A2 (en) * | 2023-03-17 | 2024-09-26 | Nanostring Technologies, Inc. | Nucleic acid probes for combined sequencing and spatial analysis |
| CN120254253A (zh) * | 2025-03-28 | 2025-07-04 | 无锡市人民医院 | 一种单细胞检测方法 |
| CN121087128B (zh) * | 2025-11-11 | 2026-02-06 | 祥符实验室 | 一种基于光控自组装的单链dna分子量标准及其制备方法 |
Family Cites Families (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| ATE497018T1 (de) * | 2000-05-19 | 2011-02-15 | Eragen Biosciences Inc | Materialien und methoden zum nachweis von nukleinsäuren |
| AU2003213652A1 (en) * | 2002-03-01 | 2003-09-16 | Integrated Dna Technologies, Inc. | Polynomial amplification of nucleic acids |
| US9045522B2 (en) * | 2006-07-31 | 2015-06-02 | Wanli Bi | Nucleic acid amplification using a reversibly modified oligonucleotide |
| US9371348B2 (en) * | 2006-11-27 | 2016-06-21 | The Trustees Of The University Of Pennsylvania | Photocleavable oligonucleotide and uses thereof |
| KR20130022437A (ko) * | 2011-08-22 | 2013-03-07 | 삼성전자주식회사 | 광분해성 화합물을 사용한, 비특이적 증폭의 감소를 위한 신규한 ｐｃｒ 방법 |
| KR20150095755A (ko) * | 2012-12-11 | 2015-08-21 | 클래리언트 다이아그노스틱 서비시즈, 인크. | 염료의 광활성화 화학적 표백 |
| EP3126512B1 (en) * | 2014-02-26 | 2019-04-17 | Ventana Medical Systems, Inc. | Photo-selective method for biological sample analysis field |
| US9745618B2 (en) * | 2014-11-19 | 2017-08-29 | Roche Molecular Systems, Inc. | Photoblocked probes and methods for sequential detection of nucleic acids |
| WO2017214417A1 (en) * | 2016-06-10 | 2017-12-14 | Takara Bio Usa, Inc. | Methods and compositions employing blocked primers |
-
2020
- 2020-05-20 CN CN202080052424.6A patent/CN114467027B/zh active Active
- 2020-05-20 WO PCT/US2020/033884 patent/WO2020236992A1/en not_active Ceased
- 2020-05-20 JP JP2021569060A patent/JP7611859B2/ja active Active
- 2020-05-20 EP EP20810824.1A patent/EP3973289A4/en active Pending
-
2021
- 2021-11-19 US US17/530,932 patent/US20220282320A1/en not_active Abandoned
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