JPWO2020059675A5 - - Google Patents

Info

Publication number

JPWO2020059675A5

JPWO2020059675A5 JP2020548485A JP2020548485A JPWO2020059675A5 JP WO2020059675 A5 JPWO2020059675 A5 JP WO2020059675A5 JP 2020548485 A JP2020548485 A JP 2020548485A JP 2020548485 A JP2020548485 A JP 2020548485A JP WO2020059675 A5 JPWO2020059675 A5 JP WO2020059675A5

Authority

JP

Japan

Prior art keywords

polypeptide

channelrhodopsin

amino acid

seq

acid sequence

Prior art date

2019-09-15

Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)

Granted

Links

• Espacenet
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• 229920001184 polypeptide Polymers 0.000 claims description 59
• 102000004196 processed proteins & peptides Human genes 0.000 claims description 59
• 108090000765 processed proteins & peptides Proteins 0.000 claims description 59
• 150000001413 amino acids Chemical class 0.000 claims description 52
• 108010035848 Channelrhodopsins Proteins 0.000 claims description 50
• 235000001014 amino acid Nutrition 0.000 claims description 28
• 230000004071 biological effect Effects 0.000 claims description 19
• 241000195597 Chlamydomonas reinhardtii Species 0.000 claims description 13
• 210000004897 n-terminal region Anatomy 0.000 claims description 12
• 210000004899 c-terminal region Anatomy 0.000 claims description 10
• 102000040430 polynucleotide Human genes 0.000 claims description 10
• 108091033319 polynucleotide Proteins 0.000 claims description 10
• 239000002157 polynucleotide Substances 0.000 claims description 10
• 210000004027 cell Anatomy 0.000 claims description 9
• 235000018417 cysteine Nutrition 0.000 claims description 8
• XUJNEKJLAYXESH-UHFFFAOYSA-N cysteine Natural products SCC(N)C(O)=O XUJNEKJLAYXESH-UHFFFAOYSA-N 0.000 claims description 8
• 241000195615 Volvox Species 0.000 claims description 6
• 235000004279 alanine Nutrition 0.000 claims description 6
• 125000003295 alanine group Chemical group N[C@@H](C)C(=O)* 0.000 claims description 6
• 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 claims description 6
• 208000035475 disorder Diseases 0.000 claims description 6
• 239000013604 expression vector Substances 0.000 claims description 6
• 210000001525 retina Anatomy 0.000 claims description 6
• FWMNVWWHGCHHJJ-SKKKGAJSSA-N 4-amino-1-[(2r)-6-amino-2-[[(2r)-2-[[(2r)-2-[[(2r)-2-amino-3-phenylpropanoyl]amino]-3-phenylpropanoyl]amino]-4-methylpentanoyl]amino]hexanoyl]piperidine-4-carboxylic acid Chemical compound C([C@H](C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCCCN)C(=O)N1CCC(N)(CC1)C(O)=O)NC(=O)[C@H](N)CC=1C=CC=CC=1)C1=CC=CC=C1 FWMNVWWHGCHHJJ-SKKKGAJSSA-N 0.000 claims description 2
• WHUUTDBJXJRKMK-UHFFFAOYSA-N Glutamic acid Natural products OC(=O)C(N)CCC(O)=O WHUUTDBJXJRKMK-UHFFFAOYSA-N 0.000 claims description 2
• 206010038848 Retinal detachment Diseases 0.000 claims description 2
• 208000007014 Retinitis pigmentosa Diseases 0.000 claims description 2
• AYFVYJQAPQTCCC-UHFFFAOYSA-N Threonine Natural products CC(O)C(N)C(O)=O AYFVYJQAPQTCCC-UHFFFAOYSA-N 0.000 claims description 2
• 239000004473 Threonine Substances 0.000 claims description 2
• 239000004480 active ingredient Substances 0.000 claims description 2
• 206010064930 age-related macular degeneration Diseases 0.000 claims description 2
• 239000003814 drug Substances 0.000 claims description 2
• 235000013922 glutamic acid Nutrition 0.000 claims description 2
• 239000004220 glutamic acid Substances 0.000 claims description 2
• 208000002780 macular degeneration Diseases 0.000 claims description 2
• 238000004519 manufacturing process Methods 0.000 claims description 2
• 239000008194 pharmaceutical composition Substances 0.000 claims description 2
• 210000000608 photoreceptor cell Anatomy 0.000 claims description 2
• 230000004264 retinal detachment Effects 0.000 claims description 2
• 125000000341 threoninyl group Chemical group [H]OC([H])(C([H])([H])[H])C([H])(N([H])[H])C(*)=O 0.000 claims description 2
• 125000003275 alpha amino acid group Chemical group 0.000 claims 15
• 229940024606 amino acid Drugs 0.000 description 19
• 108090000862 Ion Channels Proteins 0.000 description 9
• 102000004310 Ion Channels Human genes 0.000 description 9
• FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 4
• HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 3
• JKMHFZQWWAIEOD-UHFFFAOYSA-N 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid Chemical compound OCC[NH+]1CCN(CCS([O-])(=O)=O)CC1 JKMHFZQWWAIEOD-UHFFFAOYSA-N 0.000 description 2
• DCXYFEDJOCDNAF-UHFFFAOYSA-N Asparagine Natural products OC(=O)C(N)CC(N)=O DCXYFEDJOCDNAF-UHFFFAOYSA-N 0.000 description 2
• 241000702421 Dependoparvovirus Species 0.000 description 2
• 239000007995 HEPES buffer Substances 0.000 description 2
• CKLJMWTZIZZHCS-REOHCLBHSA-N L-aspartic acid Chemical compound OC(=O)[C@@H](N)CC(O)=O CKLJMWTZIZZHCS-REOHCLBHSA-N 0.000 description 2
• TWRXJAOTZQYOKJ-UHFFFAOYSA-L Magnesium chloride Chemical compound [Mg+2].[Cl-].[Cl-] TWRXJAOTZQYOKJ-UHFFFAOYSA-L 0.000 description 2
• 235000009582 asparagine Nutrition 0.000 description 2
• 229960001230 asparagine Drugs 0.000 description 2
• 125000000613 asparagine group Chemical group N[C@@H](CC(N)=O)C(=O)* 0.000 description 2
• 235000003704 aspartic acid Nutrition 0.000 description 2
• OQFSQFPPLPISGP-UHFFFAOYSA-N beta-carboxyaspartic acid Natural products OC(=O)C(N)C(C(O)=O)C(O)=O OQFSQFPPLPISGP-UHFFFAOYSA-N 0.000 description 2
• AIYUHDOJVYHVIT-UHFFFAOYSA-M caesium chloride Chemical compound [Cl-].[Cs+] AIYUHDOJVYHVIT-UHFFFAOYSA-M 0.000 description 2
• HUCVOHYBFXVBRW-UHFFFAOYSA-M caesium hydroxide Chemical compound [OH-].[Cs+] HUCVOHYBFXVBRW-UHFFFAOYSA-M 0.000 description 2
• 125000000151 cysteine group Chemical group N[C@@H](CS)C(=O)* 0.000 description 2
• HNDVDQJCIGZPNO-UHFFFAOYSA-N histidine Natural products OC(=O)C(N)CC1=CN=CN1 HNDVDQJCIGZPNO-UHFFFAOYSA-N 0.000 description 2
• 125000000487 histidyl group Chemical group [H]N([H])C(C(=O)O*)C([H])([H])C1=C([H])N([H])C([H])=N1 0.000 description 2
• 230000035772 mutation Effects 0.000 description 2
• 239000013612 plasmid Substances 0.000 description 2
• 239000011780 sodium chloride Substances 0.000 description 2
• 239000000243 solution Substances 0.000 description 2
• 239000013598 vector Substances 0.000 description 2
• YMHOBZXQZVXHBM-UHFFFAOYSA-N 2,5-dimethoxy-4-bromophenethylamine Chemical compound COC1=CC(CCN)=C(OC)C=C1Br YMHOBZXQZVXHBM-UHFFFAOYSA-N 0.000 description 1
• UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 description 1
• 241000545067 Venus Species 0.000 description 1
• 239000001110 calcium chloride Substances 0.000 description 1
• 229910001628 calcium chloride Inorganic materials 0.000 description 1
• 235000011148 calcium chloride Nutrition 0.000 description 1
• 238000010276 construction Methods 0.000 description 1
• DEFVIWRASFVYLL-UHFFFAOYSA-N ethylene glycol bis(2-aminoethyl)tetraacetic acid Chemical compound OC(=O)CN(CC(O)=O)CCOCCOCCN(CC(O)=O)CC(O)=O DEFVIWRASFVYLL-UHFFFAOYSA-N 0.000 description 1
• 229910001629 magnesium chloride Inorganic materials 0.000 description 1
• 238000005259 measurement Methods 0.000 description 1
• 238000000034 method Methods 0.000 description 1
• 108091008146 restriction endonucleases Proteins 0.000 description 1
• 238000006467 substitution reaction Methods 0.000 description 1