JPWO2019199807A5 - - Google Patents
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- Publication number
- JPWO2019199807A5 JPWO2019199807A5 JP2020555861A JP2020555861A JPWO2019199807A5 JP WO2019199807 A5 JPWO2019199807 A5 JP WO2019199807A5 JP 2020555861 A JP2020555861 A JP 2020555861A JP 2020555861 A JP2020555861 A JP 2020555861A JP WO2019199807 A5 JPWO2019199807 A5 JP WO2019199807A5
- Authority
- JP
- Japan
- Prior art keywords
- amino acid
- polymerase variant
- seq
- rna polymerase
- acid sequence
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 101710137500 T7 RNA polymerase Proteins 0.000 claims 17
- 125000003275 alpha amino acid group Chemical group 0.000 claims 15
- 238000006467 substitution reaction Methods 0.000 claims 13
- 229920002477 rna polymer Polymers 0.000 claims 7
- 102000053602 DNA Human genes 0.000 claims 4
- 108020004414 DNA Proteins 0.000 claims 4
- 238000000034 method Methods 0.000 claims 3
- 108091032973 (ribonucleotides)n+m Proteins 0.000 claims 2
- 102000040650 (ribonucleotides)n+m Human genes 0.000 claims 2
- -1 nucleoside triphosphate Chemical class 0.000 claims 2
- 239000004055 small Interfering RNA Substances 0.000 claims 2
- 235000011178 triphosphate Nutrition 0.000 claims 2
- 239000001226 triphosphate Substances 0.000 claims 2
- FWMNVWWHGCHHJJ-SKKKGAJSSA-N 4-amino-1-[(2r)-6-amino-2-[[(2r)-2-[[(2r)-2-[[(2r)-2-amino-3-phenylpropanoyl]amino]-3-phenylpropanoyl]amino]-4-methylpentanoyl]amino]hexanoyl]piperidine-4-carboxylic acid Chemical compound C([C@H](C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCCCN)C(=O)N1CCC(N)(CC1)C(O)=O)NC(=O)[C@H](N)CC=1C=CC=CC=1)C1=CC=CC=C1 FWMNVWWHGCHHJJ-SKKKGAJSSA-N 0.000 claims 1
- 108091007412 Piwi-interacting RNA Proteins 0.000 claims 1
- 108091027967 Small hairpin RNA Proteins 0.000 claims 1
- 108020004459 Small interfering RNA Proteins 0.000 claims 1
- 239000013592 cell lysate Substances 0.000 claims 1
- 229940079919 digestives enzyme preparation Drugs 0.000 claims 1
- 238000004519 manufacturing process Methods 0.000 claims 1
- 108020004999 messenger RNA Proteins 0.000 claims 1
- 108091070501 miRNA Proteins 0.000 claims 1
- 239000002679 microRNA Substances 0.000 claims 1
- 239000002777 nucleoside Substances 0.000 claims 1
- 239000011535 reaction buffer Substances 0.000 claims 1
- 239000002342 ribonucleoside Substances 0.000 claims 1
- 239000002924 silencing RNA Substances 0.000 claims 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201862655747P | 2018-04-10 | 2018-04-10 | |
| US62/655,747 | 2018-04-10 | ||
| PCT/US2019/026561 WO2019199807A1 (en) | 2018-04-10 | 2019-04-09 | T7 rna polymerase variants |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2021520815A JP2021520815A (ja) | 2021-08-26 |
| JPWO2019199807A5 true JPWO2019199807A5 (enExample) | 2022-04-14 |
Family
ID=66248824
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2020555861A Withdrawn JP2021520815A (ja) | 2018-04-10 | 2019-04-09 | Ｔ７ｒｎａポリメラーゼバリアント |
Country Status (7)
| Country | Link |
|---|---|
| US (1) | US20210180034A1 (enExample) |
| EP (1) | EP3775183A1 (enExample) |
| JP (1) | JP2021520815A (enExample) |
| CN (1) | CN112218947A (enExample) |
| AU (1) | AU2019253693A1 (enExample) |
| CA (1) | CA3095620A1 (enExample) |
| WO (1) | WO2019199807A1 (enExample) |
Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP4150067A2 (en) * | 2020-05-11 | 2023-03-22 | Thermo Fisher Scientific Baltics Uab | Mutant polymerases and methods of using the same |
| CN112980811B (zh) * | 2021-03-06 | 2022-02-25 | 苏州瀚源新酶生物科技有限公司 | Rna聚合酶突变体及其应用、重组载体及其制备方法和应用、重组工程菌及其应用 |
| JP2025078889A (ja) * | 2022-02-03 | 2025-05-21 | 天野エンザイム株式会社 | 酵素製剤 |
| CN118222535B (zh) * | 2023-12-28 | 2025-01-14 | 翌圣生物科技(上海)股份有限公司 | T7 rna聚合酶突变体及其应用 |
| WO2025208142A2 (en) | 2024-03-29 | 2025-10-02 | New England Biolabs, Inc. | Compositions, kits, and methods for in vitro transcription |
| GB202406294D0 (en) | 2024-05-06 | 2024-06-19 | Thermo Fisher Scientific Baltics Uab | Methods and compositions for producing capped mRNA |
| CN118374471A (zh) * | 2024-05-08 | 2024-07-23 | 珍奥集团股份有限公司 | 一种不产生免疫原性副产物、具备高转录活性的t7 rna聚合酶突变体与应用 |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE60110564T2 (de) * | 2000-03-07 | 2006-01-19 | bioMérieux B.V. | Rna polymerase mutanten mit erhöhter thermostabilität |
| GB0611444D0 (en) * | 2006-06-09 | 2006-07-19 | Medical Res Council Technology | Rnase H2 complex and genes therefor |
| US9193959B2 (en) * | 2010-04-16 | 2015-11-24 | Roche Diagnostics Operations, Inc. | T7 RNA polymerase variants with enhanced thermostability |
| EP2912180A1 (en) * | 2012-10-29 | 2015-09-02 | Technische Universität Dortmund | T7 rna polymerase variants and methods of using the same |
| AU2015231031A1 (en) * | 2014-03-20 | 2016-09-22 | The Board Of Regents Of The University Of Texas System | T7 RNA polymerase variants with expanded substrate range and enhanced transcriptional yield |
| CN108779446B (zh) * | 2016-01-13 | 2023-04-21 | 新英格兰生物实验室公司 | T7 rna聚合酶的热稳定变体 |
-
2019
- 2019-04-09 US US17/046,665 patent/US20210180034A1/en not_active Abandoned
- 2019-04-09 CA CA3095620A patent/CA3095620A1/en active Pending
- 2019-04-09 AU AU2019253693A patent/AU2019253693A1/en not_active Abandoned
- 2019-04-09 WO PCT/US2019/026561 patent/WO2019199807A1/en not_active Ceased
- 2019-04-09 CN CN201980025099.1A patent/CN112218947A/zh active Pending
- 2019-04-09 JP JP2020555861A patent/JP2021520815A/ja not_active Withdrawn
- 2019-04-09 EP EP19719092.9A patent/EP3775183A1/en not_active Withdrawn
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