JPWO2018194111A1 - Oral biofilm remover and oral composition - Google Patents

Abstract

An oral biofilm remover comprising (a) a polyacrylate having a weight average molecular weight of 1,000 or more and 20,000 or less, and (b) at least one selected from an anionic surfactant and an amphoteric surfactant. The above-mentioned oral biofilm remover wherein (a) / (b) is more preferably 0.005 to 2 as a mass ratio. The oral composition containing the components (a) and (b), more preferably the oral composition wherein (a) / (b) has a mass ratio of 0.005 to 2. ADVANTAGE OF THE INVENTION According to this invention, the biofilm removal agent which gives the outstanding biofilm removal effect and has a good feeling of use, and the composition for oral cavity containing this can be provided.

Description

  The present invention relates to an oral biofilm remover and an oral composition containing the same.

  Oral diseases are caused by pathogenic bacteria. These pathogenic bacteria form plaque (plaque) on the tooth surface and colonize the oral cavity, expressing pathogenicity. Therefore, to prevent oral diseases such as periodontal disease, Plaque control is very important. Means of plaque control include plaque formation suppression and sterilization, and the most important is plaque removal. In order to remove plaque chemically, not only bacterial extracellular metabolites such as glucan and protein, but also bacterial aggregates, and biofilms composed of bacterial aggregates and extracellular metabolites It is very important to remove structures effectively.

  A biofilm removal effect by using an anionic surfactant α-olefin sulfonate or alkyl sulfoacetate in combination with an enzyme has been reported (Patent Document 1; Japanese Patent Application Laid-Open No. 2015-20970).

JP, 2015-20970, A Japanese Patent Publication No. 7-29907 JP-A-10-287537 JP-A-2002-47160

  The present invention has been made in view of the above circumstances, and an object of the present invention is to provide a new oral biofilm remover that provides an excellent biofilm removal effect and an oral composition containing the same.

  The present inventors have conducted intensive studies to achieve the above object, and as a result, a relatively low molecular weight polyacrylate having a weight average molecular weight of a specific value or less, an anionic surfactant or an amphoteric surfactant. It has been found that when combined with, an excellent oral biofilm removing action is achieved. That is, in the present invention, (a) a polyacrylate having a weight average molecular weight of 1,000 or more and 20,000 or less and (b) one or more selected from anionic surfactants and amphoteric surfactants are used in combination. By doing so, it has been found that an oral biofilm remover having an excellent biofilm removing effect and a good feeling in use can be obtained. Furthermore, when the components (a) and (b) are blended with the oral composition, an excellent biofilm removing effect is obtained even if the amount of the component (a) is relatively small, and a good feeling in use (oral irritation) And no odor) can be imparted, and the present invention has been accomplished.

As a binder for oral compositions, polyacrylic acid or a salt thereof having a weight average molecular weight of 100,000 or more, usually about 300,000 is used. In addition, a polyacrylic acid polymer having a low molecular weight (molecular weight of about 4,000 to 5,500) has an anti-calculus effect, and the compounding amount in the oral composition is preferably about 2.5% or more. Is proposed in Patent Document 2 (Japanese Patent Publication No. 7-29907), and a specific example using polyacrylic acid is shown. On the other hand, surfactants have a cleaning, penetrating, and dispersing action, and although they are recognized as having a slight plaque removing action, their action on biofilms is not sufficient. May cause irritation and reduced taste. On the other hand, in the present invention, (a) a polyacrylate having a weight average molecular weight of 1,000 to 20,000 is converted from (b) an anionic surfactant and an amphoteric surfactant among the surfactants. By combining with one or more selected ones, surprisingly, both interact specifically, and the biofilm removing action by the component (b) is remarkably improved, and an excellent biofilm removing effect can be imparted. At the same time, the component (a) suppressed the deterioration of oral irritation and odor caused by the component (b), and the sensation during use was well maintained. When the component (a), which is a polyacrylate having a weight average molecular weight of 20,000 or less, was simply used, a biofilm removing effect was hardly observed (see Comparative Examples described later).
As described above, a biofilm is a structure that is composed of bacterial aggregates and extracellular metabolites and is firmly constructed and exhibits high resistance. In addition, the biofilm, which is a highly resistant deposit, was peeled off from the tooth surface and dispersed in the oral cavity, and the biofilm could be removed at a high rate. The effect of the present invention is unique to the combination system of the components (a) and (b). As shown in the comparative examples described later, polyacrylic acid and polyacrylate having an inappropriate weight average molecular weight are used. When used in combination with the component (b), the biofilm removal effect was poor.

  Patent Document 3 (Japanese Patent Application Laid-Open No. 10-287537) discloses a method of suppressing hydroxyapatite crystal formation by a polycarboxylic acid or a salt thereof derived from a polysaccharide having anhydrous glucose as a constituent unit. Japanese Patent No. 47160/1992) is a method for suppressing irritation derived from phenoxyethanol and the like by a polyacrylate. In the experimental examples, sodium polyacrylate having a molecular weight of about 50,000 is used. Patent Documents 3 and 4 do not mention biofilm removal. From Patent Documents 3 and 4, improvement in the biofilm removal effect by using the components (a) and (b) of the present invention in combination cannot be predicted.

Accordingly, the present invention provides the following oral biofilm remover and oral composition.
[1]
(A) a polyacrylate having a weight average molecular weight of 1,000 to 20,000,
(B) An oral biofilm remover comprising at least one selected from anionic surfactants and amphoteric surfactants.
[2]
The oral biofilm remover according to [1], wherein (a) / (b) has a mass ratio of 0.005 to 2.
[3]
The oral biofilm remover according to [2], wherein (a) / (b) is 0.02 to 1 in terms of mass ratio.
[4]
The anionic surfactant is selected from an alkyl sulfate having an alkyl group having 12 to 14 carbon atoms, an acyl amino acid salt and an acyl taurine salt, and the amphoteric surfactant is an acyl amino acetic acid having an acyl group having 12 to 14 carbon atoms. The oral biofilm remover according to any one of [1] to [3], which is selected from betaine and fatty acid amidopropyl betaine.
[5]
(A) a polyacrylate having a weight average molecular weight of 1,000 to 20,000,
(B) an oral composition comprising at least one selected from an anionic surfactant and an amphoteric surfactant;
[6]
The oral composition according to [5], wherein (a) / (b) has a mass ratio of 0.005 to 2.
[7]
The oral composition according to [5] or [6], wherein the content of the component (a) is 0.01 to 2% by mass and the content of the component (b) is 0.5 to 3% by mass.
[8]
The oral composition according to any one of [5] to [7], which has a pH of 5 to 9 at 25 ° C.
[9]
The oral composition according to any one of [5] to [8], which is a dentifrice composition.

  ADVANTAGE OF THE INVENTION According to this invention, the excellent biofilm removal effect is given, and the oral biofilm remover with a good feeling of use and the composition for oral cavity containing this can be provided. The oral biofilm remover and the oral composition of the present invention are effective for preventing or suppressing periodontal disease.

  The oral biofilm remover of the present invention comprises: (a) a polyacrylate having a weight average molecular weight of 1,000 to 20,000, and (b) an anionic surfactant or an amphoteric surfactant. More than one species is the active ingredient.

The polyacrylate salt of the component (a) has a weight average molecular weight (Mw) of 1,000 or more and 20,000 or less. In this case, the weight average molecular weight is 1,000 or more, and 20,000 or less, preferably 10,000 or less, from the viewpoint of a biofilm removing effect. When the weight average molecular weight is less than 1,000, the effect of removing the biofilm is inferior. If it exceeds 20,000, the effect of removing the biofilm decreases, and a sufficient effect cannot be obtained.
The weight average molecular weight was measured by GPC (gel permeation chromatography) under the method and measurement conditions described in Japanese Patent No. 5,740,859. The details are shown below.
Method for measuring weight average molecular weight;
The weight average molecular weight is a value measured using a gel permeation chromatograph / multi-angle laser light scattering detector (GPC-MALLS), and the conditions are as follows.
Mobile phase: 0.3 M NaClO ₄
NaN ₃ aqueous solution column: TSKgelα-M 2 precolumn: TSKguardcolumn α
Standard substance: polyethylene glycol

The polyacrylate is preferably a linear polyacrylate from the viewpoint of biofilm removal effect.
As the salt, a monovalent salt is preferable, an alkali metal salt or an ammonium salt is more preferable, and an alkali metal salt, particularly a sodium salt, is more preferable.
When a polyacrylic acid salt or polyacrylic acid other than the component (a) is used instead of the component (a), the biofilm removal rate is poor even when used in combination with the component (b). In some cases, the properties may become strong, or the odor or taste may worsen, and the object of the present invention is not achieved.
A commercially available product can be used as such a polyacrylate.

  The component (b) is one or more selected from an anionic surfactant (b1) and an amphoteric surfactant (b2). Even when the component (b1) or (b2) is used, the components (b1) and (b2) The component (b2) may be used, but from the viewpoint of feeling in use, it is preferable to include at least the anionic surfactant (b1). Only the component (b1) is used, or the components (b1) and (b2) are used. It is preferable to use components in combination.

Examples of the anionic surfactant (b1) include alkyl sulfates having preferably 12 to 14 carbon atoms, particularly 12 alkyl groups, acyl amino acid salts, and acyl taurine salts. The acyl group of the acyl amino acid salt and the acyl taurine salt preferably has 12 to 14 carbon atoms, and more preferably 12 carbon atoms.
Specifically, examples of the alkyl sulfate include lauryl sulfate and myristyl sulfate. Examples of the acyl amino acid salt include acyl glutamates such as lauroyl glutamate and myristoyl glutamate, and acyl sarcosine salts such as lauroyl sarcosine salt. Examples of the acyltaurine salt include lauroylmethyltaurine salt. The salt is preferably an alkali metal salt such as a sodium salt and a potassium salt. These can be used alone or in combination of two or more, but alkyl sulfate, acyl sarcosine salt and acyl taurine salt are particularly preferable. Among them, an anionic surfactant having a hydrocarbon group having 12 carbon atoms (lauryl group) is preferable, and an alkyl sulfate (sodium salt) is more preferable because it is more excellent in taste than other surfactants. .

  As the amphoteric surfactant (b2), betaine type is preferable, and acylaminoacetic acid betaine having an acyl group having 12 to 14 carbon atoms and fatty acid amidopropyl betaine are exemplified. As the acylaminoacetic acid betaine, one having an acyl group of 12 to 14 carbon atoms is preferable, and lauroyldimethylaminoacetic acid betaine is exemplified. As the fatty acid amidopropyl betaine, coconut oil fatty acid amidopropyl betaine is exemplified. These can be used alone or in combination of two or more, and betaine acylaminoacetate is particularly preferred. Among them, those having a hydrocarbon group having 12 carbon atoms (lauryl group) are preferable, and betaine lauryldimethylaminoacetate is more preferable.

  (A) / (b) indicating the quantitative ratio of the component (a) to the component (b) is preferably 0.005 to 2, more preferably 0.005 to 1, more preferably 0.02 as a mass ratio. To 1, particularly preferably 0.03 to 0.5. When the content is within the above range, the biofilm removing effect is more excellent, and the odor and taste are better and the feeling of use is further improved. When it is 0.005 or more, the biofilm removing effect is further improved, and when it is 2 or less, a good smell and taste of use can be sufficiently maintained.

  The oral biofilm remover of the present invention can be obtained by using the components (a) and (b) together as an active ingredient and blending the aforementioned ingredients. In addition, although it can be used as an oral biofilm remover consisting of only the above-mentioned active ingredient, if necessary, it may further contain other optional ingredients known for oral use. Can be blended in a range that does not hinder.

  The oral composition of the present invention contains the components (a) and (b) as active ingredients. The oral composition is specifically a paste, gel or liquid dentifrice composition (toothpaste, gel-like dentifrice, liquid dentifrice, liquid dentifrice, etc.), mouthwash, mouth spray, coating, patch And these can be prepared. Among them, toothpaste is preferred.

  In this case, in the present invention, the components (a) and (b), which are active ingredients for removing oral biofilms, can be defined by the above specific ratio. From the viewpoints of the film removing effect and the feeling upon use, the amounts of the components (a) and (b) are preferably in the ranges described below, and it is preferable to use both components at concentrations satisfying these.

  The compounding amount of the component (a) is preferably from 0.01 to 2% (% by mass, the same applies hereinafter) of the whole composition, more preferably from 0.01 to 1%, and still more preferably from 0.05 to 0.5%. It is. When the content is 0.01% or more, a sufficient biofilm removing effect can be obtained. When it is at most 2%, the odor and taste can be maintained well and sufficiently. If the amount is too large, the smell and taste may deteriorate and the feeling of use may be inferior.

  The amount of component (b) is preferably 0.5 to 3%, more preferably 0.5 to 2%, even more preferably 1.0 to 2.0% of the whole composition. If it is 0.5% or more, a sufficient biofilm removing effect can be obtained. When the content is 3% or less, oral irritation can be sufficiently suppressed, and odor and taste can be maintained well and sufficiently. If the amount is too large, the oral stimulus may become strong, or the odor or taste may be deteriorated, resulting in inferior usability.

  The oral composition of the present invention may further contain, if necessary, known components according to the dosage form and the like as optional components. The optional components are preferably added within a range that does not impair the effects of the present invention. Specifically, dentifrice compositions include abrasives, binders, thickeners, nonionic surfactants and cationic surfactants as surfactants, as well as sweeteners, preservatives, active ingredients, and pigments. , A fragrance can be blended, and these components can be mixed with water to produce.

  Examples of the abrasive include dibasic calcium hydrogen phosphate / anhydride and dihydrate, tertiary calcium phosphate, monobasic calcium phosphate, calcium carbonate, calcium pyrophosphate, aluminum hydroxide, alumina, silicic anhydride, aluminum silicate, insoluble Sodium metaphosphate, tribasic magnesium phosphate, magnesium carbonate, magnesium sulfate, bentonite, zirconium silicate, polymethyl metaphosphate, and other synthetic resins can be blended (the blending amount is usually 5 to 60%, and 10% for toothpaste. ~ 55%).

  In particular, paste-like dentifrice compositions such as toothpastes include sodium alginate as a binder, alginic acid derivatives such as propylene glycol alginate, xanthan gum, tragacanth gum, gela gum, gum karaya, gums such as gum arabic, carrageenan, carboxymethyl cellulose. Cellulose derivatives such as sodium, methylcellulose, hydroxyethylcellulose, sodium carboxymethylhydroxyethylcellulose, organic binders such as polyvinyl alcohol, carboxyvinyl polymer, and polyvinylpyrrolidone, and inorganic binders such as silica gel, aluminum silica gel, veegum, and laponite can be blended. (The compounding amount is usually 0.3 to 10%).

  Furthermore, especially in paste or liquid dentifrice compositions, sugar alcohols such as sorbitol, maltitol, lactitol, erythritol and polyhydric alcohols such as propylene glycol can be blended as a thickener. Alternatively, two or more kinds can be blended (the blending amount is usually 5 to 70%).

Nonionic surfactants include sugar fatty acid esters such as sucrose fatty acid esters; sugar alcohol fatty acid esters such as maltitol fatty acid esters; polyoxyethylene sorbitan fatty acid esters such as polyoxyethylene sorbitan monostearate; polyoxyethylene cured castor Polyoxyethylene fatty acid esters such as oil; fatty acid diethanolamide such as lauric acid mono- or diethanolamide; and polyoxyethylene higher alcohol ethers can be blended. Among them, polyoxyethylene fatty acid esters, in particular, polyoxyethylene hydrogenated castor oil having an addition mole number (EO, the same applies hereinafter) of ethylene oxide of 20 to 100, particularly 20 to 80, is effective in removing biofilms. More preferred.
Examples of the cationic surfactant include an alkyl ammonium salt such as distearyl methyl ammonium chloride and an alkyl benzyl ammonium salt such as stearyl dimethyl benzyl ammonium chloride.
The total amount of the nonionic surfactant and the cationic surfactant is preferably 0.01 to 10%.
In the present invention, it is preferable to mix a nonionic surfactant with the component (b) as the surfactant. When a nonionic surfactant, particularly polyoxyethylene hydrogenated castor oil, is used in combination with the component (a), the odor and taste may be deteriorated depending on the amount of addition. However, when a nonionic surfactant is added together with the component (b), Thus, the biofilm removing effect is further improved without deteriorating the feeling of use.

  Sweetening agents include saccharin sodium, stevioside, dipotassium glycyrrhizinate, perillartin, thaumatin, neohesperidyl dihydrochalcone, and asparatyl phenylalanine methyl ester. Examples of preservatives include p-hydroxybenzoate and sodium benzoate.

  Active ingredients include enzymes such as dextranase, mutanase, lysozyme, amylase, protease, lytic enzyme, and SOD (superoxide dismutase); alkali metal monofluorophosphates such as sodium monofluorophosphate and potassium monofluorophosphate; Fluorides such as sodium fluoride and stannous fluoride; tranexamic acid, epsilon aminocaproic acid, aluminum chlorohydroxyl allantoin, dihydrocholestanol, glycyrrhizic acid, glycyrrhetinic acid, glycerophosphate, chlorophyll, sodium chloride, xylitol, zinc chloride, Examples include water-soluble inorganic phosphates and vitamins such as vitamin A, vitamin B group, vitamin C and vitamin E. These active ingredients can be used alone or in combination of two or more, and can be blended in an effective amount within a range not to impair the effects of the present invention.

Flavors include peppermint oil, spearmint oil, anise oil, eucalyptus oil, wintergreen oil, cassia oil, clove oil, thyme oil, sage oil, lemon oil, orange oil, peppermint oil, cardamom oil, coriander oil, mandarin oil, lime Oil, lavender oil, rosemary oil, laurel oil, camomill oil, caraway oil, marjoram oil, bay oil, lemongrass oil, origanum oil, pine needle oil, neroli oil, rose oil, jasmine oil, iris concrete, absolute peppermint , Fragrances such as Absolute Rose, Orange Flower, etc., and fragrances that have been processed (cut into front reservoir, cut into rear reservoir, fractionation, liquid-liquid extraction, essence, powdered fragrance, etc.) Menthol, carvone, anethole, methyl salicylate, cinnami Qualdehyde, 3-l-menthoxypropane-1,2-diol, linalool, linalool acetate, limonene, menthone, menthyl acetate, N-substituted-paramentan-3-carboxamide, pinene, octylaldehyde, citral, pulegone, cal Beer acetate, anisaldehyde, ethyl acetate, ethyl butyrate, allyl cyclohexane propionate, methyl anthranilate, ethyl methyl phenyl glycidate, vanillin, undecalactone, hexanal, isoamyl alcohol, hexenol, dimethyl sulfide, cycloten, furfural, Single flavors such as trimethylpyrazine, ethyl lactate, and ethyl thioacetate, as well as strawberry flavor, apple flavor, banana flavor, Ingredients such as inapple flavor, grape flavor, mango flavor, butter flavor, milk flavor, fruit mix flavor, tropical fruit flavor, and the like can be used, and known flavor materials used in oral compositions can be used. It is not limited to.
In addition, the above-mentioned perfume material is preferably used in 0.000001 to 1% of the whole composition. It is preferable to use 0.001 to 2.0% as a fragrance for flavoring using the above fragrance material in a pharmaceutical composition.

  The pH (25 ° C.) of the oral composition is preferably 5 to 9, more preferably 6 to 8. If the pH is too low, there is a risk of oral irritation and demineralization of enamel and dentin. If the pH is too high, oral irritation and a decrease in formulation stability may occur.

  In addition, you may add a pH adjuster. As the pH adjuster, an inorganic acid, an organic acid or a salt thereof can be used. For example, acetic acid, hydrochloric acid, sulfuric acid, nitric acid, citric acid, phosphoric acid, malic acid, gluconic acid, maleic acid, succinic acid, glutamic acid, sodium hydroxide, potassium hydroxide, sodium acetate, sodium carbonate, sodium citrate, citric acid Sodium hydrogen, sodium phosphate and sodium dihydrogen phosphate.

  The oral composition of the present invention can be used in an aluminum tube, a laminated tube in which both surfaces of an aluminum foil are laminated with plastic or the like, a plastic tube, or a predetermined container such as a bottle-shaped container or an aerosol container.

Hereinafter, the present invention will be described specifically with reference to Examples and Comparative Examples, but the present invention is not limited to the following Examples. In the following examples,% indicates mass% unless otherwise specified.
The weight average molecular weight is a value measured using a gel permeation chromatograph / multi-angle laser light scattering detector (GPC-MALLS), and the conditions are as follows.
Mobile phase: 0.3 M NaClO ₄
NaN ₃ aqueous solution column: TSKgelα-M 2 precolumn: TSKguardcolumn α
Standard substance: polyethylene glycol The pH of the composition is a value at 25 ° C.

[Examples and Comparative Examples]
A dentifrice composition (toothpaste) having the composition shown in Tables 1 to 3 was prepared by the following method, filled in a container (aluminum laminated tube), and evaluated by the following method. The results are shown in the table.

<Preparation method>
(1) The component (a), other water-soluble components, and a viscosity modifier were mixed and dissolved at room temperature in purified water (mixture X).
(2) The binder was dispersed in propylene glycol at room temperature (mixture Y), and the mixture Y was added and mixed with the mixture X being stirred to prepare a mixture Z.
(3) In the mixture Z, the fragrance, the component (b), and the abrasive were mixed at room temperature using a kneader, and defoaming was performed under reduced pressure (5.3 kPa) to obtain a dentifrice composition.
In addition, the dentifrice composition of the comparative example was prepared according to the said method.

<Evaluation method of biofilm removal effect>
(1) Method for Producing Model Biofilm Bacteria used for producing a model biofilm were purchased from American Type Culture Collection (ATCC) and precultured by the following method.
Actinomyces viscosus ATCC43146, Fusobacterium nucleatum ATCC10953, Porphyromonas gingivalis (Porphymonas gingivalis) and 5mg / L MGK / L MGK / L MGK / GLK MGL MGMG27 / Cultivated in a culture medium [THBHM] (Becton and Dickinson) containing Todd hevit broth (manufactured by Becton and Dickinson), and Veylonella parvula ATCC 17745 contains 1.26% sodium lactate (Sigma). (Becton and Dickins They were cultured by n Co., Ltd.) culture [THBL]. The culture was performed at 37 ° C. overnight under anaerobic culture (80 vol% nitrogen, 10 vol% carbon dioxide, 10 vol% hydrogen).
After the culture, the bacterial solution was collected by centrifugation (10,000 rpm, 10 minutes). Each of the bacteria collected by centrifugation was resuspended in a basal medium mucin culture solution [BMM] ^{* 1,} and then the number of bacteria was reduced to 1 × 10 ⁷ cells / mL in a culture tank containing 1,000 mL of the same medium in advance. The cells were inoculated so as to be cultured at 37 ° C. under anaerobic conditions (95 vol% nitrogen, 5 vol% carbon dioxide) overnight. Thereafter, BMM was supplied at a rate of 100 mL / hour, and the culture solution was discharged at the same rate. The culture solution discharged from the culture tank was continuously supplied to another culture tank in which the liquid volume was maintained at 300 mL. A 7 mm-diameter hydroxyapatite plate (manufactured by PENTAX) was attached as a carrier for attaching a biofilm to a rotating disk (rotated at about 80 rpm) in the culture tank.
Culture by the above method was performed continuously for 10 days, and a biofilm was formed on a hydroxyapatite plate. After the culturing, the biofilm-formed hydroxyapatite plate taken out was washed twice with 5 mL of phosphate buffered saline ^{* 2} (Phosphate Buffered Saline, hereinafter referred to as PBS) to obtain a model biofilm.

* 1: BMM composition (expressed in mass per liter)
Proteose peptone (Becton and Dickinson): 4 g / L
Tryptone (Becton and Dickinson):
2g / L
Yeast extract (Becton and Dickinson):
2g / L
Mucin (manufactured by Sigma): 5 g / L
Hemin (Sigma): 2.5 mg / L
Vitamin K (manufactured by Wako Pure Chemical Industries): 0.5 mg / L
KCl (manufactured by Wako Pure Chemical Industries, Ltd.): 1 g / L
Cysteine (manufactured by Wako Pure Chemical Industries, Ltd.): 0.2 g / L
Distilled water: residual

* 2: Composition of PBS (expressed in mass per liter)
NaCl (manufactured by Wako Pure Chemical Industries, Ltd.): 8.0 g
KCl (manufactured by Wako Pure Chemical Industries, Ltd.): 0.2 g
Na ₂ HPO ₄ .12H ₂ O (manufactured by Wako Pure Chemical Industries, Ltd.): 3.63 g
KH ₂ PO ₄ (manufactured by Wako Pure Chemical Industries, Ltd.): 0.24 g
Distilled water: residue (adjusted to pH 7.4 with 1N HCl and made up to 1 L in total volume)

(2) Evaluation method of removal effect of model biofilm The model biofilm-formed hydroxyapatite plate prepared in (1) was transferred to a 24-well multiplate (manufactured by Sumitomo Bakelite). To this, the dentifrice composition prepared by the above method as a test composition was diluted 3-fold with artificial saliva (50 mM KCl, 1 mM CaCl ₂ , 0.1 mM MgCl ₂ , 1 mM KH ₂ PO ₄ , pH 7.0). 2 mL of the centrifugal supernatant (10,000 rpm, 10 minutes) of the agent solution was added and immersed for 3 minutes. Thereafter, the residue was washed six times with 1 mL of PBS (manufactured by Wako Pure Chemical Industries, Ltd.) and subjected to ultrasonic treatment (200 μA, 10 seconds) in a test tube (diameter 13 mm × 100 mm) to which 2 mL of the same buffer was added as described above. The film was forced to disperse. The turbidity (OD) of this dispersion at a wavelength of 550 nm was measured, and the remaining amount of the biofilm was measured.
The biofilm removal effect of the test composition was determined by calculating the removal rate relative to the control by the following formula, and from this removal rate, the oral biofilm removal effect was determined according to the following criteria.
A control similarly treated with 2 ml of PBS instead of the dentifrice solution was used as a control.
Biofilm removal rate (%) =
{(Turbidity of control−turbidity of test composition treated product) / turbidity of control} × 100
Criteria for biofilm removal effect ：: Biofilm removal rate is 90% or more 以上: Biofilm removal rate is 70% or more and less than 90% △: Biofilm removal rate is 50% or more and less than 70% ×: Biofilm removal rate Less than 50%

<How to evaluate usability>
Ten test subjects evaluated the following stimuli of oral irritation and odor (no oral irritation, no odor) as a feeling of use when 1 g of the dentifrice composition was placed on a toothbrush and brushed for 3 minutes to wash the inside of the mouth. The evaluation was based on criteria. The average of the evaluation points of 10 persons was calculated and judged according to the following criteria.

Evaluation criteria for oral irritation 4 points: no irritation in the oral cavity 3 points: level slightly in the oral cavity but no problem 2 points: irritation in the oral cavity 1 point: very irritation in the oral cavity Oral irritation criterion ◎: Average score of 3.0 or more ：: Average score of 2.5 or more and less than 3.0 Δ: Average score of 1.5 or more and less than 2.5 ×: Average score of 1.5 Less than the score Odor evaluation criteria 4 points: No unpleasant odor is felt in the oral cavity 3 points: A level of unpleasant odor is slightly felt in the oral cavity, but there is no problem 2 points: An unpleasant odor is felt in the oral cavity 1 point: In the oral cavity A strong smell is felt in the odor. Criteria for odor ◎: Average score of 3.5 or more ○: Average score of 3.0 or more and less than 3.5 △: Average score of 2.0 or more and less than 3.0 ×: Average point less than 2.0

The details of the sodium polyacrylate and polyacrylic acid used are shown below.
(A) Sodium polyacrylate (Mw 1,000): manufactured by Polyscience Co., Ltd. (a) Sodium polyacrylate (Mw 6,000): manufactured by Toagosei Co., Ltd.
AC-10NP
(A) Sodium polyacrylate (Mw 8,000): manufactured by Polyscience (a) Sodium polyacrylate (Mw 20,000): manufactured by Toagosei Co., Ltd., Alon A-20UN
Sodium polyacrylate (Mw 300,000, comparative product): Polyacrylic acid (Mw 6,000, comparative product): Alon A, manufactured by Toagosei Co., Ltd.
-10SL

Claims (9)

(A) a polyacrylate having a weight average molecular weight of 1,000 to 20,000,
(B) An oral biofilm remover comprising at least one selected from anionic surfactants and amphoteric surfactants.   The oral biofilm remover according to claim 1, wherein (a) / (b) is 0.005 to 2 in terms of mass ratio.   The oral biofilm remover according to claim 2, wherein (a) / (b) is 0.02 to 1 in terms of mass ratio.   The anionic surfactant is selected from an alkyl sulfate having an alkyl group having 12 to 14 carbon atoms, an acyl amino acid salt and an acyl taurine salt, and the amphoteric surfactant is an acyl amino acetic acid having an acyl group having 12 to 14 carbon atoms. The oral biofilm remover according to any one of claims 1 to 3, which is selected from betaine and fatty acid amidopropyl betaine. (A) a polyacrylate having a weight average molecular weight of 1,000 to 20,000,
(B) an oral composition comprising at least one selected from an anionic surfactant and an amphoteric surfactant;   The oral composition according to claim 5, wherein (a) / (b) is 0.005 to 2 in terms of mass ratio.   The oral composition according to claim 5 or 6, wherein the content of the component (a) is 0.01 to 2% by mass, and the content of the component (b) is 0.5 to 3% by mass.   The oral composition according to any one of claims 5 to 7, which has a pH of 5 to 9 at 25 ° C.   The oral composition according to any one of claims 5 to 8, which is a dentifrice composition.