JPWO2018070459A1 - Plant growth promoter and plant cultivation method - Google Patents

Abstract

According to the present invention, a plant growth promoter comprising a phosphorylated oligosaccharide polyvalent metal salt as an active ingredient, and a plant comprising applying the phosphorylated oligosaccharide polyvalent metal salt to a plant, preferably spraying it on the leaves of the plant A cultivation method is provided. The plant growth promoter and plant cultivation method of the present invention are highly safe, and can improve and / or prevent plant rot.

Description

Reference to related applications

  This patent application is accompanied by a claim of priority based on Japanese Patent Application No. 2016-200533 (filing date: October 12, 2016), which has been previously filed. The entire disclosure of the prior patent application is hereby incorporated by reference as part of the disclosure of the present invention.

  The present invention relates to a novel plant growth promoter and a plant cultivation method using the same.

  Conventionally, livestock manure combustion ash such as chicken manure combustion ash is used as a fertilizer raw material because it contains components such as phosphorus and potassium. Moreover, as a fertilizer raw material, a chemical conversion fertilizer obtained by reacting phosphoric acid with plant ash, and a chemical conversion fertilizer obtained by reacting phosphoric acid with chicken manure combustion ash are also used.

  In recent years, consumers have become increasingly aware of food safety. With regard to agricultural products, too, their safety has attracted attention, and organic agricultural products and low agricultural chemicals such as organic vegetables grown without using chemically synthesized fertilizers and agricultural chemicals are attracting attention. Moreover, in the cultivation process of agricultural products, a cultivation method to reduce the use of chemical synthetic fertilizers and pesticides as much as possible is considered.

  By the way, in the cultivation process of agricultural products, additional fertilization is often performed. In particular, for promoting the growth of plants, calcium-containing spraying agents and chemical fertilizers are conventionally used.

  On the other hand, phosphorylated oligosaccharide calcium salt is a compound known as potato-derived phosphorylated sugar. Phosphorylated oligosaccharide calcium salt is sold as a food material, and is also added to food and drink (for example, Patent Document 1). Phosphorylated oligosaccharide calcium salt is known to have an action of promoting calcium absorption to the living body as a calcium fortifying agent and promoting remineralization of teeth. In recent years, addition of phosphorylated oligosaccharide calcium salt to cultivation solutions of bean sprouts and mushrooms has been studied to improve the texture of bean sprouts and to increase the mineral component of mushrooms (Patent Document 2). And 3).

JP, 2002-253164, A JP, 2009-268463, A International Publication No. 2013/191159

Spraying agents adhere to and remain on edible parts such as fruits of plants, and their safety is important. In addition, calcium-containing drugs and chemical fertilizers tend to form poorly soluble salts, and their use in soil may be a problem, so their use may be limited.
On the other hand, examples in which the phosphorylated oligosaccharide calcium salts described in Patent Documents 1 to 3 are used for promoting the growth of plants have not been known conventionally.

  MEANS TO SOLVE THE PROBLEM In order to solve the subject of said prior art, the present inventors advanced examination for the purpose of providing a plant growth promoter with high safety | security and the plant cultivation method which can promote the growth of a plant.

As a result of intensive studies to solve the above problems, the present inventors found that phosphorylated oligosaccharide polyvalent metal salts mainly known as food materials can promote the growth of plants. The present invention has been completed.
Specifically, the present invention provides the following [1] to [13].

[1] A plant growth promoter containing a phosphorylated oligosaccharide polyvalent metal salt as an active ingredient.
[2] The plant growth promoter according to [1], wherein the phosphorylated oligosaccharide polyvalent metal salt is a phosphorylated oligosaccharide calcium salt.
[3] The plant growth promoter according to [1] or [2], which is an aqueous solution containing 1.00 g / L or more and 4.00 g / L or less phosphorylated oligosaccharide calcium salt.
[4] The plant growth promoter according to any one of [1] to [3], which is a plant growth regulator.
[5] The plant growth promoter according to any one of [1] to [3], which is an agent for improving and / or preventing butt rot.
The composition for plant cultivation containing the plant growth promoter in any one of [6] [1]-[5].
[7] A method of cultivating a plant, which comprises spraying the phosphorylated oligosaccharide polyvalent metal salt on a plant.
[8] The plant cultivation method according to [7], wherein the phosphorylated oligosaccharide polyvalent metal salt is a phosphorylated oligosaccharide calcium salt.
[9] The plant cultivation method according to [7] or [8], wherein the phosphorylated oligosaccharide polyvalent metal salt is dispersed on the leaf surface of the plant.
[10] The plant cultivation method according to [9], wherein an aqueous solution containing a phosphorylated oligosaccharide calcium salt having a concentration of 1.00 g / L or more and 4.00 g / L or less is dispersed.
[11] A method for ameliorating and / or preventing buttock rot, comprising applying to the plant an improvement and / or a prophylactically effective amount of buttock rot of a phosphorylated oligosaccharide polyvalent metal salt.
[12] Use of phosphorylated oligosaccharide polyvalent metal salt for promoting plant growth.
[13] Use of phosphorylated oligosaccharide polyvalent metal salt for ameliorating and / or preventing butt rot of a plant.

From still another aspect, according to the present invention,
Use of phosphorylated oligosaccharide polyvalent metal salts for plant growth regulation;
Use of a phosphorylated oligosaccharide polyvalent metal salt for the production of the above-mentioned plant growth promoter;
Use of a phosphorylated oligosaccharide polyvalent metal salt for the preparation of the above-mentioned plant growth regulator;
Use of a phosphorylated oligosaccharide polyvalent metal salt for the preparation of an agent for improving and / or preventing buttock rot;
Phosphorylated oligosaccharide polyvalent metal salts for use as plant growth promoters;
Phosphorylated oligosaccharide polyvalent metal salt for use as a plant growth regulator;
Phosphorus oligosaccharide polyvalent metal salt for use as an agent for ameliorating and / or preventing buttock rot;
A method of promoting plant growth, comprising applying a plant growth promoting effective amount of a phosphorylated oligosaccharide polyvalent metal salt to a plant; and a method of regulating plant growth, a phosphorylated oligosaccharide polyvalent metal A method is provided comprising applying a plant growth regulating effective amount of salt to a plant.

  According to the present invention, a plant growth promoter comprising a phosphorylated oligosaccharide polyvalent metal salt as an active ingredient is provided. Phosphorylated oligosaccharide polyvalent metal salts are also used as food materials. Therefore, the plant growth promoter of the present invention is highly safe. The present invention also provides a method for cultivating plants using phosphorylated oligosaccharide polyvalent metal salts. The growth method of the present invention can promote the growth of plants. In addition, since the phosphorylated oligosaccharide polyvalent metal salt is a food material, the safety when it is used as a spraying agent or fertilizer in the plant cultivation process is extremely high.

FIG. 1 is a graph showing the tail rot rate of each individual in Examples 1 to 3 and Comparative Example 1 and Reference Example 1. It is a graph which shows the average mass per fruit in Example 1, comparative example 1, and reference example 1. It is a graph which shows the average yield increase per seedling in Example 1, Comparative Example 1, and Reference Example 1.

  Hereinafter, the present invention will be described in detail. The description of the configuration requirements described below may be made based on typical embodiments and specific examples, but the present invention is not limited to such embodiments.

(Plant growth promoter)
The plant growth promoter of the present invention contains phosphorylated oligosaccharide polyvalent metal salt as an active ingredient.
In the present specification, “plant growth promotion” includes promotion of plant growth (increase of plant height and mass), improvement of fruit set rate (increase of fruit set rate), increase of fruit mass, germination promotion, rooting The meaning of promotion etc. is included, and also the meaning of increase in yield as a crop is also included. "Plant growth promotion" may be achieved by promoting plant growth by addition of nutrient components or promotion of absorption of nutrient components, etc., and is achieved by suppression of diseases that inhibit plant growth. It may be. That is, the plant growth promoter may be an agent for improving and / or preventing a disease that affects the growth of plants. The plant growth promoting agent may be, for example, the below-mentioned end rot disease as a disease affecting the growth of plants. In the present specification, “plant growth promotion” can be reworded as “plant growth regulation”. That is, the plant growth promoter may be a plant growth regulator.

In the present specification, "include as an active ingredient" means to include as a main active ingredient, and to mean to contain to the extent that an effect can be obtained.
As used herein, "amelioration and / or prevention" means "amelioration", "prevention", or "amelioration and prevention". For example, the "improvement and / or prevention agent" may function only as an improvement agent, may only function as a prevention agent, and may simultaneously have functions as an improvement agent and a prevention agent. .
In the present specification, "amelioration of plant end rot" means reduction and alleviation of symptoms exhibited by a plant having end rot, and for example, the use of the plant growth promoter of the present invention makes the end concave. It means reduction of the number of fruits which are blackening, or reduction of the degree of depression of the end of the fruits or reduction of the degree of blackening. In addition, "prevention of plant bottom rot" means to prevent the occurrence of symptoms exhibited by plants having hip rot.

  The phosphorylated oligosaccharide polyvalent metal salt is a raw material used for food and is sold as a food material. In the present invention, a highly safe plant growth promoter can be provided by using a phosphorylated oligosaccharide polyvalent metal salt that can also be used as a food.

  The plant growth promoter of the present invention can be used in the cultivation of general plants such as agricultural products and flowers. Among them, the plant growth promoter of the present invention is preferably used for crop cultivation, and more preferably used for fruit plant cultivation. As a fruit plant, Preferably a Solanaceous plant is mentioned, More preferably, a Solanum genus and a red pepper genus are mentioned, For example, a Solanum tomato, a eggplant, a red pepper genus pepper, a paprika etc. can be mentioned.

  For example, in the process of cultivating tomatoes, due to rapid growth of tomato fruits, nutrient supply from the roots may not be in time, and the phenomenon in which the ends of tomato fruits are dented or blackened may be observed. Such a phenomenon is called so-called "tail rot" and is known as a disease that inhibits the growth of plants, particularly crops such as solanaceous fruit. In addition, in both tomato and paprika, a local calcium deficiency has been confirmed as a cause of rear end rot (Morley et al., 1993; Adams and Ho, 1992; LFM Marcelis LC Ho, Journal of Experimental Botany, Vol. 50, No. 332, pp. 357-363, March 1999). The plant growth promoter of the present invention is capable of suppressing the occurrence of the above-mentioned "tail rot". That is, the plant growth promoter of the present invention can be used as an agent for improving and / or preventing hip rot. In order to prevent growth inhibition of fruits due to "tail rot", conventionally, it has been practiced to spray a chemical such as a calcium agent in the cultivation process or to additionally fertilize a chemical fertilizer containing calcium. However, conventionally used medicines and fertilizers are not sold as food materials, and therefore, the safety of cultivated fruits remains a problem. Since the plant growth promoter of the present invention is highly safe, as described later, it can also be used as a spraying agent which may be attached to fruits and the like. In addition, the plant growth promoter of the present invention has high solubility in water, rapid decomposition by microorganisms in the soil, and further does not contain nitrate nitrogen, so residual to the use soil is less likely to be a problem.

  When using the plant growth promoter of this invention in the process of growing a tomato, a plant growth promoter is sprayed on the seedling of a plant, for example. It is preferable that the plant growth promoter be sprayed multiple times in the process of growth. Through such a spraying step, a plant growth promoter may be attached to tomato fruits, or the plant growth promoter may be accumulated in the use soil. However, since the plant growth promoter of the present invention contains a phosphorylated oligosaccharide polyvalent metal salt having high safety to the extent that it can be used for food, the plant growth promoter may be attached to tomato fruits, or plant growth Even if the promoter is accumulated in the use soil, there is no problem in the safety of the tomato fruit and the safety of the use soil. Furthermore, as in the case of chemical fertilizers, the formation of poorly soluble salts is also less likely to occur and the accumulation in soil is also less. In addition, when agricultural workers and the like spray plant growth promoters, even if they inhale plant growth promoters without intention, they do not affect the human body because they are food materials. Thus, the plant growth promoter of the present invention is extremely safe.

  The plant growth promoter of the present invention works to promote the growth of fruit plants. For example, it is possible to improve the fruit number of fruit plants or to improve the mass per fruit. By using the plant growth promoter of the present invention, it is possible to promote the growth of fruit plants and further enhance the cultivation efficiency. Therefore, the plant growth promoter of the present invention can also be referred to as a fruit setting improvement agent or a harvest extender.

  In addition, the plant growth promoter of the present invention may also have the function of improving the taste of grown fruits or enhancing specific components. For example, when phosphooligosaccharide calcium salt is used as phosphooligosaccharide polyvalent metal salt, calcium concentration contained in individual plant may be increased, and calcium-enhanced vegetables and fruits may be obtained.

  Furthermore, the plant growth promoter of the present invention may also serve to reduce nitrate nitrogen concentration in grown plants. For example, fruits with reduced bitterness and astringency may be obtained by cultivation using the plant growth promoter of the present invention. It is presumed that the phosphorus contained in the phosphorylated oligosaccharide polyvalent metal salt works to improve the sugar content in the fruit.

  The plant growth promoter of the present invention can also be used for cultivating leafy vegetables and for cultivating florets. As leafy vegetables, for example, lettuce, cabbage, Chinese cabbage and the like can be mentioned. As a flower bud, you may list, for example, Eustoma grandiflorum, Delphium and the like.

  The plant growth promoter of the present invention may consist only of phosphorylated oligosaccharide polyvalent metal salt, and may contain other components as optional components. Other ingredients include metal salts. As a metal salt, calcium chloride, iron chloride, copper chloride, zinc chloride, manganese chloride etc. can be mentioned, for example.

  The plant growth promoter of the present invention can also be provided as a plant cultivation composition containing phosphorylated oligosaccharide polyvalent metal salt and other components. The composition for plant cultivation may contain an agriculturally usable excipient, and preferably comprises a foodable excipient from the viewpoint of safety. Excipients include, for example, starch, cellulose, sucrose, natural gums, lignin, lignin derivatives, furthermore clay, talc, amorphous silicon dioxide. The composition for plant cultivation may further contain an additive such as a pigment, a diluent, a pH adjuster, or a stabilizer. The composition for plant cultivation may further contain water, soil, other known fertilizers and the like.

  The plant growth promoter of the present invention may be solid or liquid. The plant growth promoter of the present invention may be a solution dissolved in water or other solvent. The solution is preferably an aqueous solution because it can be dispersed. The plant growth promoter of the present invention may be one in which the phosphorylated oligosaccharide polyvalent metal salt is dissolved in water or another solvent and then concentrated. In addition, the plant growth promoter of the present invention may be a powder. For example, an aqueous solution containing a phosphorylated oligosaccharide polyvalent metal salt and optionally other components may be concentrated and dried to form a powder.

  The plant growth promoter of the present invention is preferably transported as a powder. It is because the cost reduction at the time of transportation is possible. In this case, it can be dissolved in water or a solvent as needed at the time of use. By transporting it in powder form, the solution transport makes it possible to reduce the disadvantage of causing precipitation and inhomogeneity.

  When the plant growth promoter of the present invention is a liquid, specifically a solution, the plant growth promoter of the present invention can be preferably used as a spraying agent. In particular, the plant growth promoter of the present invention can be absorbed through leaves by using it as a foliar spray. Furthermore, in the case of liquid, accumulation in the working soil can be reduced as compared to solid.

  When a plant growth promoter is used as a spraying agent, the plant growth promoter of the present invention is preferably used as a solution containing an active ingredient at an appropriate concentration. For example, it is preferable to use a solution in which the concentration of polyvalent metal salt of phosphorylated oligosaccharide polyvalent metal salt at the time of spraying is 1.00 g / L or more and 4.00 g / L or less, more preferably 1.5 g / L. L or more and 3.5 g / L or less, more preferably 1.7 g / L or more and 3.0 g / L or less.

  The plant growth promoter of the present invention may be stored as a solution having a concentration suitable for spraying, and may be stored in a concentrated state. The plant growth promoter of the present invention may be stored in the form of powder or gel, and may be used in the form of powder or gel.

  The plant growth promoter of the present invention may be permeabilized by plants. In this case, the plant growth promoter of the present invention may be solid or liquid. Moreover, it may be provided as a chemical | medical agent for spraying (it is also called "a spreading agent"), and may be provided in the state mixed with the use earth. The plant growth promoter of the present invention may also be provided as a fertilizer. In addition, it may be provided as a plant cultivation substrate carrying a plant growth promoter on a carrier or the like.

(Phosphoric oligosaccharide polyvalent metal salt)
The phosphorylated oligosaccharide polyvalent metal salt is a compound in which a partially phosphorylated oligosaccharide is bonded to a polyvalent metal. The oligosaccharide referred to here is one in which 2 to 100 monosaccharides are linked. The degree of polymerization of the oligosaccharide monosaccharide is preferably 50 or less, more preferably 30 or less, still more preferably 10 or less, and particularly preferably 8 or less. For example, oligo and the like in the phosphorylated oligosaccharide polyvalent metal salt are preferably those in which 2 or more and 10 or less monosaccharides are bound, and more preferably 2 or more and 8 or less monosaccharides. The amount of phosphate group introduced into the oligosaccharide is preferably 1 or more and 10 or less, more preferably 1 or more and 5 or less, and 1 or 2 per phosphorylated oligosaccharide molecule. It is more preferable that

  The molecular weight of the phosphorylated oligosaccharide is preferably 400 or more, more preferably 500 or more, still more preferably 600 or more, and particularly preferably 700 or more. The molecular weight of the phosphorylated oligosaccharide is preferably 10000 or less, more preferably 2000 or less, and still more preferably 1000 or less. For example, the molecular weight of the phosphorylated oligosaccharide may be 400 or more and 10000 or less, 500 or more and 2000 or less, or 600 or more and 1000 or less.

  Since the phosphate group of the phosphorylated oligosaccharide has a negative charge, polyvalent metal ions having a positive charge form a salt with the phosphate group. Examples of the polyvalent metal include at least one selected from calcium, magnesium, iron, zinc, copper, vanadium, manganese, cobalt, nickel, aluminum, selenium and molybdenum. Among them, at least one selected from calcium, magnesium, iron and zinc is preferable, and calcium is particularly preferable. The phosphorylated oligosaccharide polyvalent metal salt is preferably a salt containing a single polyvalent metal, but may be a complex salt containing two or more polyvalent metals.

  Phosphorylated oligosaccharide polyvalent metal salt includes phosphorylated oligosaccharide calcium salt (POs-Ca), phosphorylated oligosaccharide magnesium salt (POs-Mg), phosphorylated oligosaccharide iron salt (POs-Fe) and phosphorylated oligosaccharide It is preferable to be selected from the group consisting of zinc salts (POs-Zn), and it is particularly preferable to be phosphorylated oligosaccharide calcium salt (POs-Ca). As phosphorylated oligosaccharide calcium salt (POs-Ca), for example, POs-Ca 50 manufactured by Ezaki Glico Co., Ltd. can be used. Phosphorylated oligosaccharide calcium salts have high solubility and are suitable for use as a form of a spray and the like.

  The solubility of the phosphorylated oligosaccharide calcium salt (POs-Ca) is preferably 500 g / L or more, more preferably 600 g / L or more, and still more preferably 700 g / L or more. Since the phosphorylated oligosaccharide calcium salt (POs-Ca) has high solubility, it is suitable for use as a hydroponic cultivation or a spray.

  The number of polyvalent metal ions contained in the phosphorylated oligosaccharide polyvalent metal salt is not particularly limited, and polyvalent metal ions may be bound to all of the phosphate groups present in the phosphorylated oligosaccharide, The polyvalent metal ion may be bound only to the part. For example, only one polyvalent metal ion may be present in one phosphorylated oligosaccharide molecule, or two or three or more polyvalent metal ions may be present. The upper limit value of the polyvalent metal ion present in one phosphorylated oligosaccharide molecule can be, for example, 20 or less. For example, 3 or more and 20 or less polyvalent metal ions may be present in one phosphorylated oligosaccharide molecule.

(Method for producing phosphorylated oligosaccharide multivalent metal salt)
The phosphorylated oligosaccharide polyvalent metal salt is produced by phosphorylating known saccharides.
It is preferable that glucan is used as saccharides which are the manufacturing materials of phosphorylated oligosaccharide polyvalent metal salt. As the glucan, for example, starch having a large amount of phosphate groups such as potato crude starch bound is suitable. Also, modified starch is preferably used. Furthermore, it is also possible to use various carbohydrates to which a phosphate group is chemically bonded. In potato starch, relatively many phosphate groups are ester-linked at the 3- and 6-positions of the glucose constituting the potato starch. The phosphate groups are mainly present in amylopectin.

  When the saccharide is a glucan, a phosphated oligosaccharide polyvalent metal salt can be obtained by decomposing starch having a phosphate group or modified starch. For the degradation, a starch degrading enzyme, a glycosyltransferase, an α-glucosidase and the like can be used, and these may be used alone or in combination of two or more. However, when using (alpha)-glucosidase, using in combination with another enzyme is preferable.

  The starch degrading enzyme is preferably at least one selected from the group consisting of α-amylase, β-amylase, glucoamylase, isoamylase, pullulanase, and neopullulanase. Also, the glycosyltransferase is preferably cyclodextrin glucanotransferase.

  After the step of decomposing the starch having a phosphoric acid group or the modified starch, it is preferable to provide a separation and purification step as necessary. In the separation and purification step, insoluble substances such as proteins are removed, and the oligosaccharide having a phosphate group introduced therein is separated from the oligosaccharide having no phosphate group. In order to separate an oligosaccharide into which a phosphate group has been introduced, it is preferable to carry out a separation and purification step using, for example, an anion exchange resin. The type of anion exchange resin is not particularly limited, but Chitopearl BCW 2501 type (manufactured by Fuji Spinning Co., Ltd.), Amberlite IRA type (manufactured by Organo), DEAE-cellulose (manufactured by Whatman), DEAE-Sephadex, QAE-Sephadex (Manufactured by Pharmacia), QAE-cellulose (manufactured by Bio-Rad), and the like are suitably used.

  After the separation and purification step, the phosphorylated oligosaccharide or phosphorylated oligosaccharide derivative is reacted with a polyvalent metal salt. For example, phosphorylated oligosaccharide calcium salt can be obtained by reacting water-soluble calcium salt.

  In addition, about the manufacturing method of phosphorylated sugar, the method described in Unexamined-Japanese-Patent No. 8-104696 and Unexamined-Japanese-Patent No. 10-84985 can be employ | adopted suitably.

(Plant cultivation method, ameliorating and / or preventing method of buttock rot)
The plant growth can be promoted by applying the phosphorylated oligosaccharide polyvalent metal salt to plants for plant cultivation. In addition, application of phosphorylated oligosaccharide polyvalent metal salts to plants can improve and / or prevent butt rot. In the plant cultivation method using phospho-oligosaccharide polyvalent metal salt, and the method for ameliorating and / or preventing end rot, specifically, plant growth promotion comprising the above-mentioned phospho-oligosaccharide polyvalent metal salt as an active ingredient The agent may be applied to plants.

  As a method of applying a plant growth promoter to plants, a method of spraying a plant growth promoter on a plant, a method of mixing a plant growth promoter to soil for plant cultivation, and the like can be mentioned. The plant growth promoter may be absorbed into plants by transfoliation or may be absorbed by roots. As a method of applying a plant growth promoter to a plant, a method of spraying a plant growth promoter on the foliage of a plant is preferable.

  When the plant growth promoter is applied to the plants, it is preferable that the plant growth promoter is applied regularly from the stage of planting, more preferably after fruit set and before harvest. The plant growth promoter is preferably applied to the whole plant, preferably to the leaves of the individual plant, and more preferably to both sides of the leaf. The plant growth promoter may be applied at least once, preferably twice or more, and more preferably three times or more, from the stage of plant seedling to harvest.

  The phosphorylated oligosaccharide polyvalent metal salt used for the plant cultivation method and the method for ameliorating and / or preventing end rot is preferably phosphooligosaccharide calcium salt, and in the case of spraying phosphorylated oligosaccharide calcium salt It is preferable to spray an aqueous solution containing a phosphorylated oligosaccharide calcium salt having a concentration of 1.00 g / L or more and 4.00 g / L or less. The concentration of the phosphorylated oligosaccharide calcium salt in the aqueous solution at the time of spraying is preferably 1.00 g / L or more, more preferably 1.50 g / L or more, and further preferably 1.80 g / L or more. preferable. In addition, the concentration of the phosphorylated oligosaccharide calcium salt in the aqueous solution at the time of spraying is preferably 4.00 g / L or less, more preferably 3.50 g / L or less, and 3.00 g / L or less Is more preferred, and particularly preferably 2.50 g / L or less. Specifically, the concentration of the aqueous solution containing phosphorylated oligosaccharide calcium salt used for spraying is 1.00 g / L or more and 4.00 g / L or less, 1.00 g / L or more and 3.50 g / L or less, 00 g / L to 3.00 g / L, 1.00 g / L to 2.50 g / L, 1.50 g / L to 4.00 g / L, 1.50 g / L to 3.50 g / L, 1.50 g / L to 3.00 g / L, 1.50 g / L to 2.50 g / L, 1.70 g / L to 4.00 g / L, 1.70 g / L to 3.50 g / L Below, 1.70 g / L to 3.00 g / L, 1.70 g / L to 2.50 g / L, 1.80 g / L to 4.00 g / L, 1.80 g / L to 3.50 g / L or less, 1.80 g / L or more and 3.00 g / L or less, or 1.80 g / L It is preferably less than 2.50 g / L.

  The plant to which the phosphorylated oligosaccharide polyvalent metal salt is applied may be any plant in general such as agricultural crops and flowers, but among them, a fruit plant is preferable. That is, it is preferable that the plant cultivation method of this invention is a fruit plant cultivation method. As a fruit plant, Preferably a Solanaceous plant is mentioned, More preferably, a Solanum genus and a red pepper genus are mentioned, For example, a Solanum tomato, a eggplant, a red pepper genus pepper, a paprika etc. can be mentioned.

  The features of the present invention will be more specifically described below with reference to examples and comparative examples. The materials, amounts used, proportions, treatment contents, treatment procedures, etc. shown in the following examples can be appropriately changed without departing from the spirit of the present invention. Therefore, the scope of the present invention should not be construed as limited by the specific examples shown below.

Example 1
Tomato seeds (sales company: Takii Seed Co., Ltd., variety: Momotaro) were sown in a black poly pot. After that, 5 seedlings having similar growth were selected and planted in a Wagner pot (1 / 2000a) in a vinyl house. The soil used in the Wagner pot was mixed with manure in which the calcium fertilizer was reduced by half so that it would be a condition prone to rear rot. The breakdown of the soil in Wagner Pot (12 L) is as follows. Red bead fine grain (Kanuma City) 11.2 L, 1 kg coco- tote, 160 g bark compost, 11.2 g bitter lime, 4.8 g linster 30, 11.2 g Ecolong, high lime (N / P / K = 14% / 14% / 14%) 11.2g, Ortholane granules 0.48g

  After planting, a solution (phosphorylated oligosaccharide calcium salt-containing solution) prepared so that the concentration of phosphorylated oligosaccharide calcium salt (manufactured by Ezaki Glyco Co., Ltd., POs-Ca 50) is 1.83 g / L was used as a tomato seedling Was sprayed (sprayed) throughout the The application of the phosphooligosaccharide calcium salt-containing solution was started when the first flower bunch and the second flower bunch reached, performed weekly, and was finished when the third flower bunch was harvested and analyzed. The solution containing the phosphorylated oligosaccharide calcium salt-containing solution was sprayed using an electric sprayer to an amount sufficient to drip the solution from the whole plant to all the individuals. Thus, tomato fruits were grown.

(Example 2)
The same procedure as in Example 1 was repeated except that the solution containing phosphorylated oligosaccharide calcium salt having a concentration of 2.29 g / L was similarly sprayed instead of the solution containing phosphorylated oligosaccharide calcium salt having a concentration of 1.83 g / L. Cultivated tomato fruits.

(Example 3)
The same procedure as in Example 1 was repeated except that the solution containing phosphorylated oligosaccharide calcium salt having a concentration of 3.44 g / L was similarly sprayed instead of the solution containing phosphorylated oligosaccharide calcium salt having a concentration of 1.83 g / L. Cultivated tomato fruits.

(Comparative example 1)
The tomato fruits were cultivated in the same manner as in Example 1 except that the solution containing the phosphorylated oligosaccharide calcium salt-containing solution was similarly sprayed with water, and that the fixed-planted seedlings were changed to six seedlings.

(Reference Example 1)
A tomato was prepared in the same manner as in Example 1 except that a solution prepared to have a calcium extract (manufactured by Amino Ale Chemical Laboratory Co., Ltd.) at 2.29 g / L was similarly sprayed instead of the phosphated oligosaccharide calcium salt-containing solution. Cultivated fruits.

(Evaluation)
<Check for butt rot>
Butt rot occurs in the process of growing tomato fruits. It was judged that the tail rotted when the detached part (so-called tomato fruit butt) was browning at the time of petal withdrawal.
The total number of fruit number of first to third flower clusters and the total number of butt rot of each individual in each example, comparative example and reference example were calculated, and the butt rot percentage (%) was calculated from the following equation .
Bottom rot rate (%) = total number of hip rot number / total number of fruit number x 100

  In the example, a decrease in the end rot is seen compared to the comparative example. In addition, although the fall of a tail rot rate is seen also in a reference example, the adjuvant contained in the solution used by the reference example is a chemical fertilizer, It is a thing which is not used as a foodstuff material.

<Confirmation of average mass per fruit and average yield increase per seedling>
The average weight per fruit and the average yield per seedling of Example 1, Comparative Example 1, and Reference Example 1 were confirmed. It carried out in the same cultivation area as the above-mentioned end rot confirmation.
The tomato of the sixth flower bunch was harvested from each first flower bunch (harvest period: 27 days). The number of seedlings in each plot, the number of fruits obtained, the average mass (g) per fruit and the average yield (g) per seedling are as shown in Table 2 below, FIG. 2 and FIG.

  The phosphooligosaccharide calcium confirmed a tendency to increase in average weight per fruit and average yield per seedling.

Claims (13)

  A plant growth promoter comprising a phosphorylated oligosaccharide polyvalent metal salt as an active ingredient.   The plant growth promoter according to claim 1, wherein the phosphorylated oligosaccharide polyvalent metal salt is a phosphorylated oligosaccharide calcium salt.   The plant growth promoter according to claim 1 or 2, which is an aqueous solution containing 1.00 g / L or more and 4.00 g / L or less phosphorylated oligosaccharide calcium salt.   The plant growth promoter according to any one of claims 1 to 3, which is a plant growth regulator.   The plant growth promoter according to any one of claims 1 to 3, which is an agent for improving and / or preventing butt rot.   The composition for plant cultivation containing the plant growth promoter of any one of Claims 1-5.   A plant cultivation method comprising spraying a phosphorylated oligosaccharide polyvalent metal salt to a plant.   The plant cultivation method according to claim 7, wherein the phosphorylated oligosaccharide polyvalent metal salt is a phosphorylated oligosaccharide calcium salt.   The plant cultivation method according to claim 7 or 8, wherein the phosphorylated oligosaccharide polyvalent metal salt is dispersed on the leaf surface of the plant.   The plant cultivation method according to claim 9, wherein an aqueous solution containing a phosphorylated oligosaccharide calcium salt having a concentration of 1.00 g / L or more and 4.00 g / L or less is dispersed.   A method for ameliorating and / or preventing buttock rot, which comprises applying to the plant an improvement and / or a prophylactically effective amount of buttock rot of a phosphorylated oligosaccharide polyvalent metal salt.   Use of phosphorylated oligosaccharide polyvalent metal salt for promoting plant growth.   Use of phosphorylated oligosaccharide polyvalent metal salt for amelioration and / or prevention of butt rot of plant.