JPS6485091A - Isolation and purification of activated human protein c using antibody - Google Patents

Isolation and purification of activated human protein c using antibody

Info

Publication number
JPS6485091A
JPS6485091A JP63093450A JP9345088A JPS6485091A JP S6485091 A JPS6485091 A JP S6485091A JP 63093450 A JP63093450 A JP 63093450A JP 9345088 A JP9345088 A JP 9345088A JP S6485091 A JPS6485091 A JP S6485091A
Authority
JP
Japan
Prior art keywords
human protein
activated human
adsorbent
calcium ion
purification
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
JP63093450A
Other languages
Japanese (ja)
Inventor
Kenji Wakabayashi
Yoshihiko Washimi
Yataro Ichikawa
Yoichi Sakata
Nobuo Aoki
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Teijin Ltd
Original Assignee
Teijin Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Teijin Ltd filed Critical Teijin Ltd
Priority to JP63093450A priority Critical patent/JPS6485091A/en
Publication of JPS6485091A publication Critical patent/JPS6485091A/en
Pending legal-status Critical Current

Links

Abstract

PURPOSE:To enable the very easy isolation and purification of activated human protein C of high purity by allowing a specific monoclonal antibody to bond to the activated human protein C. CONSTITUTION:A mixture containing activated human protein C is brought into contact with an adsorbent in which an insoluble support is bonded to an antibody specifically recognizing human protein C bonding to calcium ion in the Gla domain in the presence of calcium ion to bond the activated protein C linking to calcium ion to the adsorbent. Then, the adsorbent is treated with a substantially calcium ion-free solution such as a solution containing EDTA to separate the activated human protein C from the adsorbent.
JP63093450A 1987-04-17 1988-04-18 Isolation and purification of activated human protein c using antibody Pending JPS6485091A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP63093450A JPS6485091A (en) 1987-04-17 1988-04-18 Isolation and purification of activated human protein c using antibody

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP9337787 1987-04-17
JP63093450A JPS6485091A (en) 1987-04-17 1988-04-18 Isolation and purification of activated human protein c using antibody

Publications (1)

Publication Number Publication Date
JPS6485091A true JPS6485091A (en) 1989-03-30

Family

ID=26434760

Family Applications (1)

Application Number Title Priority Date Filing Date
JP63093450A Pending JPS6485091A (en) 1987-04-17 1988-04-18 Isolation and purification of activated human protein c using antibody

Country Status (1)

Country Link
JP (1) JPS6485091A (en)

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS60120825A (en) * 1983-10-18 1985-06-28 Fujisawa Pharmaceut Co Ltd Antiprotein c monoclonal antibody and hybridoma producing the same
JPS61283868A (en) * 1985-06-10 1986-12-13 Teijin Ltd Measurement of monoclonal antibody and human protein c

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS60120825A (en) * 1983-10-18 1985-06-28 Fujisawa Pharmaceut Co Ltd Antiprotein c monoclonal antibody and hybridoma producing the same
JPS61283868A (en) * 1985-06-10 1986-12-13 Teijin Ltd Measurement of monoclonal antibody and human protein c

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