JPS6485091A - Isolation and purification of activated human protein c using antibody - Google Patents
Isolation and purification of activated human protein c using antibodyInfo
- Publication number
- JPS6485091A JPS6485091A JP63093450A JP9345088A JPS6485091A JP S6485091 A JPS6485091 A JP S6485091A JP 63093450 A JP63093450 A JP 63093450A JP 9345088 A JP9345088 A JP 9345088A JP S6485091 A JPS6485091 A JP S6485091A
- Authority
- JP
- Japan
- Prior art keywords
- human protein
- activated human
- adsorbent
- calcium ion
- purification
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Abstract
PURPOSE:To enable the very easy isolation and purification of activated human protein C of high purity by allowing a specific monoclonal antibody to bond to the activated human protein C. CONSTITUTION:A mixture containing activated human protein C is brought into contact with an adsorbent in which an insoluble support is bonded to an antibody specifically recognizing human protein C bonding to calcium ion in the Gla domain in the presence of calcium ion to bond the activated protein C linking to calcium ion to the adsorbent. Then, the adsorbent is treated with a substantially calcium ion-free solution such as a solution containing EDTA to separate the activated human protein C from the adsorbent.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP63093450A JPS6485091A (en) | 1987-04-17 | 1988-04-18 | Isolation and purification of activated human protein c using antibody |
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP9337787 | 1987-04-17 | ||
JP63093450A JPS6485091A (en) | 1987-04-17 | 1988-04-18 | Isolation and purification of activated human protein c using antibody |
Publications (1)
Publication Number | Publication Date |
---|---|
JPS6485091A true JPS6485091A (en) | 1989-03-30 |
Family
ID=26434760
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP63093450A Pending JPS6485091A (en) | 1987-04-17 | 1988-04-18 | Isolation and purification of activated human protein c using antibody |
Country Status (1)
Country | Link |
---|---|
JP (1) | JPS6485091A (en) |
Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS60120825A (en) * | 1983-10-18 | 1985-06-28 | Fujisawa Pharmaceut Co Ltd | Antiprotein c monoclonal antibody and hybridoma producing the same |
JPS61283868A (en) * | 1985-06-10 | 1986-12-13 | Teijin Ltd | Measurement of monoclonal antibody and human protein c |
-
1988
- 1988-04-18 JP JP63093450A patent/JPS6485091A/en active Pending
Patent Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS60120825A (en) * | 1983-10-18 | 1985-06-28 | Fujisawa Pharmaceut Co Ltd | Antiprotein c monoclonal antibody and hybridoma producing the same |
JPS61283868A (en) * | 1985-06-10 | 1986-12-13 | Teijin Ltd | Measurement of monoclonal antibody and human protein c |
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