JPS6459058A - Enzyme immune sensor and enzyme immunoassay using said sensor - Google Patents

Enzyme immune sensor and enzyme immunoassay using said sensor

Info

Publication number
JPS6459058A
JPS6459058A JP62215016A JP21501687A JPS6459058A JP S6459058 A JPS6459058 A JP S6459058A JP 62215016 A JP62215016 A JP 62215016A JP 21501687 A JP21501687 A JP 21501687A JP S6459058 A JPS6459058 A JP S6459058A
Authority
JP
Japan
Prior art keywords
antibody
antigen
enzyme
sensor
immobilized
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP62215016A
Other languages
Japanese (ja)
Other versions
JPH059740B2 (en
Inventor
Toshihide Kuriyama
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
NEC Corp
Original Assignee
NEC Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by NEC Corp filed Critical NEC Corp
Priority to JP62215016A priority Critical patent/JPS6459058A/en
Publication of JPS6459058A publication Critical patent/JPS6459058A/en
Publication of JPH059740B2 publication Critical patent/JPH059740B2/ja
Granted legal-status Critical Current

Links

Abstract

PURPOSE:To provide an enzyme immune sensor which can detect a slight amt. of antigen or antibody by using a flat plate immobilized with an antibody or antigen and a semiconductor ion sensor with is disposed at a slight spacing from the flat plate. CONSTITUTION:The antibody or antigen is immobilized to the flat plate 1 and the immobilized surface is immersed in a sample to be measured to effect an antigen-antibody reaction. This immobilized plate is then immersed into a soln. contg. the antibody or antigen labeled with enzyme which brings the antigen or antibody in the sample into specific reaction by which the reaction is effected. The flat plate 1 is installed in such a manner that the immobilized surface faces the surface of the semiconductor ion sensor 3 at the slight spacing maintained therebetween. The soln. contg. the substrate decomposed or oxidized by the above-mentioned labeling enzyme is filled into this spacing. The change in the concn. of ions in the soln. generated by the reaction of the substrate is measured by the ion sensor 3 and the quantity of the antigen or antibody in the sample is measured.
JP62215016A 1987-08-31 1987-08-31 Enzyme immune sensor and enzyme immunoassay using said sensor Granted JPS6459058A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP62215016A JPS6459058A (en) 1987-08-31 1987-08-31 Enzyme immune sensor and enzyme immunoassay using said sensor

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP62215016A JPS6459058A (en) 1987-08-31 1987-08-31 Enzyme immune sensor and enzyme immunoassay using said sensor

Publications (2)

Publication Number Publication Date
JPS6459058A true JPS6459058A (en) 1989-03-06
JPH059740B2 JPH059740B2 (en) 1993-02-05

Family

ID=16665329

Family Applications (1)

Application Number Title Priority Date Filing Date
JP62215016A Granted JPS6459058A (en) 1987-08-31 1987-08-31 Enzyme immune sensor and enzyme immunoassay using said sensor

Country Status (1)

Country Link
JP (1) JPS6459058A (en)

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH01212347A (en) * 1988-02-19 1989-08-25 Kuraray Co Ltd Method and apparatus for measuring very small amount of analyte substance
JPH02287144A (en) * 1989-04-27 1990-11-27 Kuraray Co Ltd Instrument for measuring trace analyte material
US5328847A (en) * 1990-02-20 1994-07-12 Case George D Thin membrane sensor with biochemical switch
JPH08166388A (en) * 1994-12-14 1996-06-25 Nec Corp Method and sensor for immunoassay of enzyme
JP2017058320A (en) * 2015-09-18 2017-03-23 Ｎｌｔテクノロジー株式会社 Biosensor and detector
WO2023042657A1 (en) * 2021-09-16 2023-03-23 株式会社デンソー Ion sensor, and method for detecting substance being detected

Cited By (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH01212347A (en) * 1988-02-19 1989-08-25 Kuraray Co Ltd Method and apparatus for measuring very small amount of analyte substance
JPH02287144A (en) * 1989-04-27 1990-11-27 Kuraray Co Ltd Instrument for measuring trace analyte material
US5328847A (en) * 1990-02-20 1994-07-12 Case George D Thin membrane sensor with biochemical switch
JPH08166388A (en) * 1994-12-14 1996-06-25 Nec Corp Method and sensor for immunoassay of enzyme
JP2017058320A (en) * 2015-09-18 2017-03-23 Ｎｌｔテクノロジー株式会社 Biosensor and detector
US10385377B2 (en) 2015-09-18 2019-08-20 Nlt Technologies, Ltd. Biosensor and detection device
US10538800B2 (en) 2015-09-18 2020-01-21 Tianma Microelectronics Co., Ltd. Biosensor and detection device
WO2023042657A1 (en) * 2021-09-16 2023-03-23 株式会社デンソー Ion sensor, and method for detecting substance being detected

Also Published As

Publication number Publication date
JPH059740B2 (en) 1993-02-05

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