JPS6359896A - 固定化リパ−ゼによる油脂加水分解法 - Google Patents
固定化リパ−ゼによる油脂加水分解法Info
- Publication number
- JPS6359896A JPS6359896A JP61205588A JP20558886A JPS6359896A JP S6359896 A JPS6359896 A JP S6359896A JP 61205588 A JP61205588 A JP 61205588A JP 20558886 A JP20558886 A JP 20558886A JP S6359896 A JPS6359896 A JP S6359896A
- Authority
- JP
- Japan
- Prior art keywords
- water
- soluble
- substance
- oily
- immobilized lipase
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 108090001060 Lipase Proteins 0.000 title claims abstract description 61
- 102000004882 Lipase Human genes 0.000 title claims abstract description 61
- 239000004367 Lipase Substances 0.000 title claims abstract description 61
- 235000019421 lipase Nutrition 0.000 title claims abstract description 61
- 238000006460 hydrolysis reaction Methods 0.000 title claims abstract description 14
- 230000007062 hydrolysis Effects 0.000 title claims abstract description 13
- 239000000126 substance Substances 0.000 claims abstract description 140
- 238000002156 mixing Methods 0.000 claims abstract description 14
- 230000003301 hydrolyzing effect Effects 0.000 claims abstract description 5
- 238000000034 method Methods 0.000 claims description 25
- 238000003541 multi-stage reaction Methods 0.000 claims description 11
- 238000006243 chemical reaction Methods 0.000 abstract description 51
- 238000000354 decomposition reaction Methods 0.000 abstract description 23
- 238000000926 separation method Methods 0.000 abstract description 19
- 101710084371 Lipase 7 Proteins 0.000 abstract description 2
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 40
- 239000000047 product Substances 0.000 description 23
- 239000003921 oil Substances 0.000 description 22
- 235000019198 oils Nutrition 0.000 description 22
- 235000011187 glycerol Nutrition 0.000 description 20
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 16
- 239000003925 fat Substances 0.000 description 14
- 108090000790 Enzymes Proteins 0.000 description 8
- 102000004190 Enzymes Human genes 0.000 description 8
- 235000014113 dietary fatty acids Nutrition 0.000 description 8
- 239000000839 emulsion Substances 0.000 description 8
- 238000002474 experimental method Methods 0.000 description 8
- 229930195729 fatty acid Natural products 0.000 description 8
- 239000000194 fatty acid Substances 0.000 description 8
- 230000007423 decrease Effects 0.000 description 7
- 239000000243 solution Substances 0.000 description 7
- 238000009792 diffusion process Methods 0.000 description 6
- 230000000694 effects Effects 0.000 description 6
- 150000004665 fatty acids Chemical class 0.000 description 6
- 239000004006 olive oil Substances 0.000 description 5
- 235000008390 olive oil Nutrition 0.000 description 5
- 230000035484 reaction time Effects 0.000 description 5
- 239000012510 hollow fiber Substances 0.000 description 4
- 239000000203 mixture Substances 0.000 description 4
- 238000003756 stirring Methods 0.000 description 4
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 3
- 108010093096 Immobilized Enzymes Proteins 0.000 description 3
- 239000002253 acid Substances 0.000 description 3
- 239000007795 chemical reaction product Substances 0.000 description 3
- -1 fatty acid esters Chemical class 0.000 description 3
- 239000012528 membrane Substances 0.000 description 3
- VLKZOEOYAKHREP-UHFFFAOYSA-N n-Hexane Chemical compound CCCCCC VLKZOEOYAKHREP-UHFFFAOYSA-N 0.000 description 3
- 239000002245 particle Substances 0.000 description 3
- IMNFDUFMRHMDMM-UHFFFAOYSA-N N-Heptane Chemical compound CCCCCCC IMNFDUFMRHMDMM-UHFFFAOYSA-N 0.000 description 2
- 239000007864 aqueous solution Substances 0.000 description 2
- OGBUMNBNEWYMNJ-UHFFFAOYSA-N batilol Chemical class CCCCCCCCCCCCCCCCCCOCC(O)CO OGBUMNBNEWYMNJ-UHFFFAOYSA-N 0.000 description 2
- 230000006866 deterioration Effects 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 239000012153 distilled water Substances 0.000 description 2
- 238000001914 filtration Methods 0.000 description 2
- 230000003100 immobilizing effect Effects 0.000 description 2
- 230000005764 inhibitory process Effects 0.000 description 2
- 238000001471 micro-filtration Methods 0.000 description 2
- 239000012454 non-polar solvent Substances 0.000 description 2
- 150000003904 phospholipids Chemical class 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 239000000758 substrate Substances 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- NHTMVDHEPJAVLT-UHFFFAOYSA-N Isooctane Chemical compound CC(C)CC(C)(C)C NHTMVDHEPJAVLT-UHFFFAOYSA-N 0.000 description 1
- 239000004743 Polypropylene Substances 0.000 description 1
- 101000697856 Rattus norvegicus Bile acid-CoA:amino acid N-acyltransferase Proteins 0.000 description 1
- CDBYLPFSWZWCQE-UHFFFAOYSA-L Sodium Carbonate Chemical compound [Na+].[Na+].[O-]C([O-])=O CDBYLPFSWZWCQE-UHFFFAOYSA-L 0.000 description 1
- DWAQJAXMDSEUJJ-UHFFFAOYSA-M Sodium bisulfite Chemical compound [Na+].OS([O-])=O DWAQJAXMDSEUJJ-UHFFFAOYSA-M 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- 230000002411 adverse Effects 0.000 description 1
- AWUCVROLDVIAJX-UHFFFAOYSA-N alpha-glycerophosphate Natural products OCC(O)COP(O)(O)=O AWUCVROLDVIAJX-UHFFFAOYSA-N 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 150000001450 anions Chemical class 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 235000015278 beef Nutrition 0.000 description 1
- 239000012295 chemical reaction liquid Substances 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 238000004132 cross linking Methods 0.000 description 1
- 230000009849 deactivation Effects 0.000 description 1
- JVSWJIKNEAIKJW-UHFFFAOYSA-N dimethyl-hexane Natural products CCCCCC(C)C JVSWJIKNEAIKJW-UHFFFAOYSA-N 0.000 description 1
- 238000004821 distillation Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 239000000835 fiber Substances 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 125000002485 formyl group Chemical class [H]C(*)=O 0.000 description 1
- 239000000499 gel Substances 0.000 description 1
- 230000005484 gravity Effects 0.000 description 1
- 230000002779 inactivation Effects 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 235000019626 lipase activity Nutrition 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 229940127554 medical product Drugs 0.000 description 1
- 235000019476 oil-water mixture Nutrition 0.000 description 1
- 238000011017 operating method Methods 0.000 description 1
- 239000003960 organic solvent Substances 0.000 description 1
- 230000003647 oxidation Effects 0.000 description 1
- 238000007254 oxidation reaction Methods 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- KHIWWQKSHDUIBK-UHFFFAOYSA-N periodic acid Chemical compound OI(=O)(=O)=O KHIWWQKSHDUIBK-UHFFFAOYSA-N 0.000 description 1
- 229920001467 poly(styrenesulfonates) Polymers 0.000 description 1
- 229920001155 polypropylene Polymers 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 238000007127 saponification reaction Methods 0.000 description 1
- AWUCVROLDVIAJX-GSVOUGTGSA-N sn-glycerol 3-phosphate Chemical compound OC[C@@H](O)COP(O)(O)=O AWUCVROLDVIAJX-GSVOUGTGSA-N 0.000 description 1
- 235000010267 sodium hydrogen sulphite Nutrition 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 238000000638 solvent extraction Methods 0.000 description 1
- 238000000967 suction filtration Methods 0.000 description 1
- 239000003760 tallow Substances 0.000 description 1
- 239000002569 water oil cream Substances 0.000 description 1
Landscapes
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP61205588A JPS6359896A (ja) | 1986-09-01 | 1986-09-01 | 固定化リパ−ゼによる油脂加水分解法 |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP61205588A JPS6359896A (ja) | 1986-09-01 | 1986-09-01 | 固定化リパ−ゼによる油脂加水分解法 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS6359896A true JPS6359896A (ja) | 1988-03-15 |
| JPH0412711B2 JPH0412711B2 (enrdf_load_stackoverflow) | 1992-03-05 |
Family
ID=16509365
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP61205588A Granted JPS6359896A (ja) | 1986-09-01 | 1986-09-01 | 固定化リパ−ゼによる油脂加水分解法 |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS6359896A (enrdf_load_stackoverflow) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5010004A (en) * | 1987-10-09 | 1991-04-23 | Agency Of Industrial Science & Technology | Method for continuous reaction with fluidized immobilized lipase |
| US6258575B1 (en) | 1998-11-26 | 2001-07-10 | Kao Corporation | Hydrolyzing fats and oils using an immobilized enzyme column and substrate-feeding chamber that separates phases |
| WO2007043552A1 (en) * | 2005-10-05 | 2007-04-19 | Kao Corporation | Method for producing a useful substance by use of an immobilized enzyme |
| US20100261235A1 (en) * | 2007-12-14 | 2010-10-14 | Kansai Chemical Engineering Co., Ltd. | Continuous production of biodiesel fuel by enzymatic method |
-
1986
- 1986-09-01 JP JP61205588A patent/JPS6359896A/ja active Granted
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5010004A (en) * | 1987-10-09 | 1991-04-23 | Agency Of Industrial Science & Technology | Method for continuous reaction with fluidized immobilized lipase |
| US6258575B1 (en) | 1998-11-26 | 2001-07-10 | Kao Corporation | Hydrolyzing fats and oils using an immobilized enzyme column and substrate-feeding chamber that separates phases |
| EP1004662A3 (en) * | 1998-11-26 | 2002-02-27 | Kao Corporation | A process for hydrolyzing fats and oils |
| WO2007043552A1 (en) * | 2005-10-05 | 2007-04-19 | Kao Corporation | Method for producing a useful substance by use of an immobilized enzyme |
| US8377664B2 (en) | 2005-10-05 | 2013-02-19 | Kao Corporation | Method for producing a useful substance by use of an immobilized enzyme |
| US20100261235A1 (en) * | 2007-12-14 | 2010-10-14 | Kansai Chemical Engineering Co., Ltd. | Continuous production of biodiesel fuel by enzymatic method |
| US9879291B2 (en) | 2007-12-14 | 2018-01-30 | Kansai Chemical Engineering Co., Ltd. | Continuous production of biodiesel fuel by enzymatic method |
Also Published As
| Publication number | Publication date |
|---|---|
| JPH0412711B2 (enrdf_load_stackoverflow) | 1992-03-05 |
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