JPS6328888B2 - - Google Patents
Info
- Publication number
- JPS6328888B2 JPS6328888B2 JP55107953A JP10795380A JPS6328888B2 JP S6328888 B2 JPS6328888 B2 JP S6328888B2 JP 55107953 A JP55107953 A JP 55107953A JP 10795380 A JP10795380 A JP 10795380A JP S6328888 B2 JPS6328888 B2 JP S6328888B2
- Authority
- JP
- Japan
- Prior art keywords
- chlorella
- fraction
- anticancer agent
- powder
- hours
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
- 241000195649 Chlorella <Chlorellales> Species 0.000 claims description 12
- 239000002246 antineoplastic agent Substances 0.000 claims description 10
- 102000003886 Glycoproteins Human genes 0.000 claims description 7
- 108090000288 Glycoproteins Proteins 0.000 claims description 7
- 235000016425 Arthrospira platensis Nutrition 0.000 claims description 4
- 240000002900 Arthrospira platensis Species 0.000 claims description 4
- 229940082787 spirulina Drugs 0.000 claims description 4
- 229940041181 antineoplastic drug Drugs 0.000 claims 2
- 241000195663 Scenedesmus Species 0.000 claims 1
- 239000000843 powder Substances 0.000 description 10
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 10
- 238000000034 method Methods 0.000 description 7
- 238000002474 experimental method Methods 0.000 description 6
- 235000018102 proteins Nutrition 0.000 description 6
- 108090000623 proteins and genes Proteins 0.000 description 6
- 102000004169 proteins and genes Human genes 0.000 description 6
- BVKZGUZCCUSVTD-UHFFFAOYSA-L Carbonate Chemical compound [O-]C([O-])=O BVKZGUZCCUSVTD-UHFFFAOYSA-L 0.000 description 4
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 4
- 239000000872 buffer Substances 0.000 description 4
- 210000004027 cell Anatomy 0.000 description 4
- 230000004083 survival effect Effects 0.000 description 4
- 238000002054 transplantation Methods 0.000 description 4
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 3
- 241000195493 Cryptophyta Species 0.000 description 3
- 206010028980 Neoplasm Diseases 0.000 description 3
- 229920005654 Sephadex Polymers 0.000 description 3
- 239000012507 Sephadex™ Substances 0.000 description 3
- 238000007796 conventional method Methods 0.000 description 3
- 239000003814 drug Substances 0.000 description 3
- 229940079593 drug Drugs 0.000 description 3
- 238000002513 implantation Methods 0.000 description 3
- 235000000346 sugar Nutrition 0.000 description 3
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 2
- 241000699670 Mus sp. Species 0.000 description 2
- 238000002835 absorbance Methods 0.000 description 2
- 238000010521 absorption reaction Methods 0.000 description 2
- 238000000862 absorption spectrum Methods 0.000 description 2
- 239000002253 acid Substances 0.000 description 2
- 150000001408 amides Chemical class 0.000 description 2
- 230000001093 anti-cancer Effects 0.000 description 2
- MSWZFWKMSRAUBD-UHFFFAOYSA-N beta-D-galactosamine Natural products NC1C(O)OC(CO)C(O)C1O MSWZFWKMSRAUBD-UHFFFAOYSA-N 0.000 description 2
- 238000000502 dialysis Methods 0.000 description 2
- 238000001962 electrophoresis Methods 0.000 description 2
- 238000005194 fractionation Methods 0.000 description 2
- 238000001502 gel electrophoresis Methods 0.000 description 2
- 230000002401 inhibitory effect Effects 0.000 description 2
- 239000012528 membrane Substances 0.000 description 2
- OQUKIQWCVTZJAF-UHFFFAOYSA-N phenol;sulfuric acid Chemical compound OS(O)(=O)=O.OC1=CC=CC=C1 OQUKIQWCVTZJAF-UHFFFAOYSA-N 0.000 description 2
- 108090000765 processed proteins & peptides Proteins 0.000 description 2
- 239000011780 sodium chloride Substances 0.000 description 2
- 239000000243 solution Substances 0.000 description 2
- MTCFGRXMJLQNBG-REOHCLBHSA-N (2S)-2-Amino-3-hydroxypropansäure Chemical compound OC[C@H](N)C(O)=O MTCFGRXMJLQNBG-REOHCLBHSA-N 0.000 description 1
- MSWZFWKMSRAUBD-GASJEMHNSA-N 2-amino-2-deoxy-D-galactopyranose Chemical compound N[C@H]1C(O)O[C@H](CO)[C@H](O)[C@@H]1O MSWZFWKMSRAUBD-GASJEMHNSA-N 0.000 description 1
- MSWZFWKMSRAUBD-IVMDWMLBSA-N 2-amino-2-deoxy-D-glucopyranose Chemical compound N[C@H]1C(O)O[C@H](CO)[C@@H](O)[C@@H]1O MSWZFWKMSRAUBD-IVMDWMLBSA-N 0.000 description 1
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- 206010008342 Cervix carcinoma Diseases 0.000 description 1
- GUBGYTABKSRVRQ-WFVLMXAXSA-N DEAE-cellulose Chemical compound OC1C(O)C(O)C(CO)O[C@H]1O[C@@H]1C(CO)OC(O)C(O)C1O GUBGYTABKSRVRQ-WFVLMXAXSA-N 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- CKLJMWTZIZZHCS-REOHCLBHSA-N L-aspartic acid Chemical compound OC(=O)[C@@H](N)CC(O)=O CKLJMWTZIZZHCS-REOHCLBHSA-N 0.000 description 1
- AYFVYJQAPQTCCC-GBXIJSLDSA-N L-threonine Chemical compound C[C@@H](O)[C@H](N)C(O)=O AYFVYJQAPQTCCC-GBXIJSLDSA-N 0.000 description 1
- 208000007093 Leukemia L1210 Diseases 0.000 description 1
- 208000028018 Lymphocytic leukaemia Diseases 0.000 description 1
- 241001529936 Murinae Species 0.000 description 1
- 241000699666 Mus <mouse, genus> Species 0.000 description 1
- 108010059712 Pronase Proteins 0.000 description 1
- 239000012980 RPMI-1640 medium Substances 0.000 description 1
- 208000006268 Sarcoma 180 Diseases 0.000 description 1
- MTCFGRXMJLQNBG-UHFFFAOYSA-N Serine Natural products OCC(N)C(O)=O MTCFGRXMJLQNBG-UHFFFAOYSA-N 0.000 description 1
- AYFVYJQAPQTCCC-UHFFFAOYSA-N Threonine Natural products CC(O)C(N)C(O)=O AYFVYJQAPQTCCC-UHFFFAOYSA-N 0.000 description 1
- 239000004473 Threonine Substances 0.000 description 1
- 208000006105 Uterine Cervical Neoplasms Diseases 0.000 description 1
- 239000003513 alkali Substances 0.000 description 1
- 230000000259 anti-tumor effect Effects 0.000 description 1
- 235000003704 aspartic acid Nutrition 0.000 description 1
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 1
- OQFSQFPPLPISGP-UHFFFAOYSA-N beta-carboxyaspartic acid Natural products OC(=O)C(N)C(C(O)=O)C(O)=O OQFSQFPPLPISGP-UHFFFAOYSA-N 0.000 description 1
- 239000007853 buffer solution Substances 0.000 description 1
- 201000011510 cancer Diseases 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- 239000001569 carbon dioxide Substances 0.000 description 1
- 229910002092 carbon dioxide Inorganic materials 0.000 description 1
- 201000010881 cervical cancer Diseases 0.000 description 1
- 238000011033 desalting Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- AOMZHDJXSYHPKS-UHFFFAOYSA-L disodium 4-amino-5-hydroxy-3-[(4-nitrophenyl)diazenyl]-6-phenyldiazenylnaphthalene-2,7-disulfonate Chemical compound [Na+].[Na+].[O-]S(=O)(=O)C1=CC2=CC(S([O-])(=O)=O)=C(N=NC=3C=CC=CC=3)C(O)=C2C(N)=C1N=NC1=CC=C([N+]([O-])=O)C=C1 AOMZHDJXSYHPKS-UHFFFAOYSA-L 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- 238000010828 elution Methods 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 210000003918 fraction a Anatomy 0.000 description 1
- 238000004108 freeze drying Methods 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 229960002442 glucosamine Drugs 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- 230000007062 hydrolysis Effects 0.000 description 1
- 238000006460 hydrolysis reaction Methods 0.000 description 1
- 238000011081 inoculation Methods 0.000 description 1
- 230000002147 killing effect Effects 0.000 description 1
- 208000003747 lymphoid leukemia Diseases 0.000 description 1
- 238000004264 monolayer culture Methods 0.000 description 1
- 239000000178 monomer Substances 0.000 description 1
- 210000003200 peritoneal cavity Anatomy 0.000 description 1
- 239000008363 phosphate buffer Substances 0.000 description 1
- 229920000642 polymer Polymers 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 238000004062 sedimentation Methods 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 238000001228 spectrum Methods 0.000 description 1
- 238000001694 spray drying Methods 0.000 description 1
- 150000008163 sugars Chemical class 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 230000001225 therapeutic effect Effects 0.000 description 1
- 238000003211 trypan blue cell staining Methods 0.000 description 1
Landscapes
- Medicines Containing Plant Substances (AREA)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP10795380A JPS5732224A (en) | 1980-08-06 | 1980-08-06 | Carcinostatic agent |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP10795380A JPS5732224A (en) | 1980-08-06 | 1980-08-06 | Carcinostatic agent |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS5732224A JPS5732224A (en) | 1982-02-20 |
| JPS6328888B2 true JPS6328888B2 (enrdf_load_stackoverflow) | 1988-06-10 |
Family
ID=14472235
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP10795380A Granted JPS5732224A (en) | 1980-08-06 | 1980-08-06 | Carcinostatic agent |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS5732224A (enrdf_load_stackoverflow) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP3677706A1 (de) | 2019-01-04 | 2020-07-08 | EXCOR Korrosionsforschung GmbH | Zusammensetzungen und verfahren zur vorbehandlung von substraten für die nachfolgende fixierung von dampfphasen-korrosionsinhibitoren |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS6219528A (ja) * | 1985-07-16 | 1987-01-28 | Kurorera Kogyo Kk | 制癌剤 |
| JPH041341Y2 (enrdf_load_stackoverflow) * | 1985-12-11 | 1992-01-17 | ||
| JPS63239231A (ja) * | 1987-03-27 | 1988-10-05 | Kurorera Kogyo Kk | 分化誘導剤 |
-
1980
- 1980-08-06 JP JP10795380A patent/JPS5732224A/ja active Granted
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP3677706A1 (de) | 2019-01-04 | 2020-07-08 | EXCOR Korrosionsforschung GmbH | Zusammensetzungen und verfahren zur vorbehandlung von substraten für die nachfolgende fixierung von dampfphasen-korrosionsinhibitoren |
Also Published As
| Publication number | Publication date |
|---|---|
| JPS5732224A (en) | 1982-02-20 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Fujii et al. | Isolation and characterization of a new antitumor polysaccharide, KS-2, extracted from culture mycelia of Lentinus edodes | |
| US4699787A (en) | Nitrogen-containing polysaccharide | |
| JPS5896025A (ja) | 新規生理活性物質ｃｈ−１およびその製造法 | |
| CA1088443A (en) | Method of producing polysaccharides | |
| EP0132125B1 (en) | A protein having antitumor activity | |
| EP0115157B1 (en) | Hypocholesterolemically active protein | |
| JPS6328888B2 (enrdf_load_stackoverflow) | ||
| RU2082755C1 (ru) | Штамм ganoderma lucidum - продуцент протеогликана gоо9, обладающий противоопухолевой и иммуностимулирующей активностью | |
| US4851395A (en) | Nitrogen-containing polysaccharide | |
| CN105418789A (zh) | 一种制备具有免疫调节和抗肿瘤活性的低分子牡蛎多糖的方法 | |
| EP0136791B1 (en) | Neocarzinostatin derivatives and method of producing the same | |
| EP0132981A2 (en) | Hypotriglyceridemically active polysaccharides | |
| JPS58128322A (ja) | 制癌剤 | |
| CN117209620A (zh) | 一种马齿苋半乳葡聚糖与其组合物及其制备方法和应用 | |
| CN113717293B (zh) | 一种软珊瑚多糖及其制备方法与应用 | |
| JPH03205402A (ja) | リグニン配糖体およびその用途 | |
| Saim et al. | Low molecular weight immunosuppressive factors found in elevated amounts in cancer ascitic fluids of mice 1. Isolation, identification and immunosuppressive effects of uric acid and uracil | |
| JPS632415B2 (enrdf_load_stackoverflow) | ||
| CN105646658B (zh) | 孔鳐软骨蛋白抗氧化肽及其用途 | |
| NO155697B (no) | Fremgangsmaate for fremstilling av fysiologisk aktive forbindelser ved fermentering. | |
| JPS6044287B2 (ja) | 制癌剤の製造法 | |
| JPS6019718A (ja) | 多糖体ｎ９ｇｉの製造法 | |
| CN118021935A (zh) | 一种暗褐网柄牛肝菌蛋白的应用 | |
| CN118638891A (zh) | 一种具有抗肿瘤功效的灵芝低聚肽及其制备方法 | |
| CN110438080A (zh) | 一种人参提取物gqr1应用于肿瘤细胞的操作方法 |