JPS6324164A - Automatic biochemical anlayzing instrument - Google Patents

Abstract

PURPOSE:To execute multi-item analysis with high exactness by diluting a vital sample at a specified dilution, then supplying a prescribed amt. thereof repeatedly to a biochemical analysis part and making the multi-item analysis. CONSTITUTION:Sample vessels 21 into which samples are introduced are successively arranged in respective sample lines 2 of an automatic biochemical analysis instrument 1. While empty dilution vessel 31 are held arranged in a dilution line 3, a dilution dispenser 7 sucks a dilution standard liquid in a position (c) and dispenses the liquid into a cuvette 61 in a position (e). The detection output obtd. in the analysis part 6 in such a manner is stored together with the concn. of the dilution standard liquid into an arithmetic part. The concns. with respective items are calculated in accordance with the concn. of the dilution standard liquid and the output in the analysis part 6. The calibration by the dilution standard liquid is executed again after the analysis is made with a prescribed number of the samples. The correction of the concn. value of the dilution standard liquid is executed for the respective items at every prescribed number of the calibrations, by which the dilution error by the dilution dispensing operation is corrected.

Description

DETAILED DESCRIPTION OF THE INVENTION Field of Industrial Application This invention relates to an automatic biochemical analyzer. More specifically, the present invention relates to a multi-item biochemical analyzer configured to dilute a biological sample at a constant ratio and then repeatedly supply a predetermined amount of the diluted sample to a biochemical analysis section to perform multi-item analysis.

(B) Conventional technology In recent years, biochemistry automatic 8 analyzers used in clinical chemistry tests have been used to dilute the sample and then sample the diluted solution and send it to the biochemical analysis department. The number of devices that supply and analyze samples is increasing, and in particular, devices that perform multi-item analysis require a relatively large amount of specimen, so such dilution type devices are being used.

Such a dilution type multi-item automatic biochemical analyzer is usually equipped with a sample line in which a plurality of sample containers are arranged and a corresponding dilution line in which a plurality of dilution containers are arranged. The sample (specimen) is poured into a dilution container using an appropriate mobile dilution dispenser at a predetermined magnification (usually 5 to 20
This diluted sample is then repeatedly supplied to the biochemical analysis section for analysis for each item measurement. As the standard solution for calibration, a diluted standard solution with a known concentration corresponding to the diluted sample is prepared in advance and used, and detection is performed when this diluted standard solution is directly supplied to the biochemical analysis department using the above-mentioned dispenser. The concentration of each item in the sample is calculated based on the output and its standard solution concentration, and this calibration is updated every predetermined analysis cycle.

(c) Problems to be solved by the invention However, since the above-mentioned dilution type analyzer uses a mechanical dilution dispensing means called a moving dilution dispenser, it is prone to errors in the dilution ratio. There is a problem in that systematic errors are likely to occur, and when analytical calculations are performed based on the diluted standard solution prepared in accordance with a fixed dilution ratio, non-negligible errors occur. In particular, micrometer samples (
For example, when an analysis is performed on about 10 samples, for example, if the dilution is set to 20 times, there is an inconvenience that the concentration error will be about ±1 to 2%.

Therefore, it is ideal to introduce an undiluted standard solution into a sample container and supply it to the analysis department through a dilution operation using a pipettor under the same conditions as the sample. There is a problem in that operations such as introducing the standard solution into the sample container, setting the set, and control mode are required every time, which is complicated.

The present invention has been made in view of this situation, and it is an object of the present invention to provide a multi-item biochemical automatic analyzer using a dilution method, which allows optimization of calibration with a particularly simple operation.

(d) Means for solving the problem According to the present invention, (a) a sample line in which a plurality of sample containers are arranged and a dilution line in which a plurality of dilution containers are arranged correspondingly; (C) A biochemical analysis section that performs multi-item analysis; A mobile dilution dispenser that can aspirate a predetermined amount of sample and repeatedly separate and supply it to the biochemical analysis department, as well as aspirate the diluted standard solution in the standard solution storage tank and supply it directly to the biochemical analysis department every predetermined analysis cycle. and a calculation unit that calculates the concentration of the test component in each sample based on the concentration of the TEL diluted standard solution and the detection output when supplied to the biochemical analysis unit, Based on the detection output A when the undiluted standard solution is introduced and supplied to the biochemical analysis department through the dilution dispensing operation using the mobile dilution dispensing means, the concentration of the diluted standard solution is calculated for each item, which is the basis for calculation. Formula: (
■) C=Co -A/B--(I>C In the formula, C is the concentration for calculation of the corrected diluted standard solution, Co
represents the actual concentration of the diluted standard solution, and B represents the detection output of the diluted standard solution.

In the device of the present invention, based on the above formula (I>),
The concentration for calculation of the diluted standard solution used for calibration is corrected every predetermined analysis cycle. In other words, the difference between the output when an undiluted standard solution is supplied to the analysis section through the dilution dispensing operation and the output when a pre-diluted standard solution is supplied is due to the setting by the dilution operation of the dilution dispensing means. In order to deal with the difference between the dilution ratio and the actual dilution ratio, the dilution standard used for calculation is calculated by multiplying the set concentration Co of the diluted standard solution by a coefficient obtained by dividing the detection output of the former by the detection output B of the latter. The liquid'a degree value can be optimized, and errors caused by dilution and dispensing operations can be eliminated. In general, such optimization operations do not need to be performed for every calibration, but for a long period of time (e.g.
It is sufficient to do this every month or so, and it does not cause any operational complications.

(F) Embodiment FIG. 1 is an explanatory diagram showing the configuration of an embodiment of the automatic biochemical analyzer of the present invention, and FIG. 2 is a plan explanatory diagram showing a part of the configuration. In the figure, the biochemical automatic analyzer 1 is
A sampling table 4 having a sample line 2 in which a plurality of sample containers 21 are arranged and a dilution line 3 in which a plurality of dilution containers 31 are arranged, a storage tank 5 for a diluted standard solution each containing a test component of a known concentration, and a multi-item It basically consists of a biochemical analysis section 6 that performs analysis, a rotationally movable dilution dispenser 7 that performs sample sampling, dilution, transfer, etc., a control section 9, and a display section 10. Note that 8 is a cleaning liquid storage tank.

The biochemical analysis section 6 includes a turntable 62 on which reaction cuvettes 61 are continuously arranged, and one or two types of reagents for measuring a predetermined item (for example, GOT, CPK, A reaction reagent dispenser 63 for selecting and dispensing a reagent for optical measurement such as P, BtJN, etc.; and a detector for detecting the absorbance at a predetermined wavelength of the reaction liquid in the reaction cuvette, which is equipped with a light source, a spectroscopic means, and a light receiver. It consists of 64. As shown in FIG. 2, the dilution dispenser 7 has a sample suction position b on the sample line, a diluted sample discharge/suction position wa on the dilution line, a diluted standard solution suction position C, and a cleaning position d of the nozzle 71. and the discharge position e into the reaction cuvette, and the nozzle is moved up and down to perform suction and discharge operations. Specifically, aspirating the sample in the sample container (position b
) → Discharge of diluted sample in the dilution container l\ (position a) → Cleaning of the nozzle (position d) → Aspiration of a part of the diluted sample in the dilution container (position a) → Discharge of the diluted sample into the reaction cuvette ( Perform the sample sampling operation consisting of position e〉 and the standard solution sampling operation consisting of suction of the diluted standard solution (position Z, c) → discharge of the diluted standard solution into the reaction cuvette (position e).The standard solution sampling operation consists of: The concentration of a predetermined item in the sample is calculated based on the ratio between the known concentration value and the detection output [for example, m degrees - output of the sample x (output of the diluted standard solution x dilution). The output of the magnification/dilution standard solution is displayed on the display unit 10.

On the other hand, the control unit 9 controls the driving of the sampling table, dilution dispenser, biochemical analysis unit, etc. with a microcomputer according to a predetermined program, and has a calculation unit that calculates the concentration of the above-mentioned predetermined items, Furthermore, it is equipped with a correction calculation section. The correction calculation unit uses the detection output obtained when performing the sample sampling operation using the undiluted standard solution instead of the sample to calculate the concentration of the diluted standard solution used as the calculation base for the concentration in the sample using the following formula (I ):% formula % [In the formula, C is the concentration for calculation of the corrected diluted standard solution, C0
Correction is made based on the actual concentration of the diluted standard solution, A the detection output of the undiluted standard solution, and B the detection output of the diluted standard solution. In addition, it is preferable to adopt the average value of multiple measurements for each of the above-mentioned numerical values, and it is usually optimal to use the average value of three measurements. These correction calculations and adoption of the average value mode are performed by external input using a keyboard or the like.

In such an automatic biochemical analyzer 1, each sample line 2
With the sample containers 21 into which samples such as serum have been introduced are arranged in sequence, and the empty dilution containers 31 are arranged in the dilution line 3, the dilution dispenser 7 dispenses the diluted standard solution and undiluted standard solution at position C. A 20-fold diluted solution) is aspirated and dispensed into the cuvette 61 at position e, and the detection output obtained by the analysis section 6 is stored in the calculation section together with the concentration of the diluted standard solution inputted from the outside. This is done for each item.

Next, after cleaning the nozzle, the sample is aspirated at position a, and the diluted sample is dispensed at position a (the set dilution factor is the same as above, 2).
After cleaning the nozzle, the diluted sample is suctioned at position a and the diluted sample is discharged (dispensed) at position e, and this is repeated until a number of diluted samples corresponding to the multiple items are transferred to Cubera 1-61. The diluted standard solution is introduced continuously, and the concentration of each item is calculated in the analysis section 6 based on the diluted standard solution'&1 degree and the output. After analyzing a predetermined number of samples, calibration is performed again using a diluted standard solution, and this calibration is also performed every predetermined analysis cycle. Concentration values are corrected for each item, and dilution errors due to dilution and dispensing operations are corrected. It is sufficient to perform such a correction once a month in routine examinations at a normal hospital, and this makes it possible to easily perform multi-item analysis with high accuracy.

(G) Effects of the Invention According to the automatic biochemical analyzer of the present invention, highly accurate multi-item analysis can be performed without introducing a standard solution into a sample container for each calibration. Therefore, it is extremely useful as an analyzer for routine testing that performs multi-item biochemical analysis of a large amount of samples.

[Brief explanation of the drawing]

FIG. 1 is a structural explanatory diagram showing one embodiment of an automatic biochemical analyzer of the present invention, and FIG. 2 is a planar structural explanatory diagram showing a part of the same. 1... Biochemical automatic analyzer, 2... Sample line, 3... Dilution line, 4...
Sampling table, 5...Standard solution storage, 6
...Biochemical analysis department, 7...Rotating and moving dilution dispenser, 9...Control section, 21...
Sample container, 31... Dilution container.

Claims (1)

[Scope of Claims] 1. (a) A sample line in which a plurality of sample containers are arranged, a dilution line in which a plurality of dilution containers are arranged correspondingly, and (
b) A standard solution storage tank for storing a diluted standard solution of known concentration;
) A biochemical analysis department that performs multi-item analysis; and (d) the sample in the sample container is aspirated, diluted and dispensed at a predetermined ratio into a dilution container, and then a predetermined amount of this diluted sample is aspirated and repeated to the biochemical analysis department. A mobile dilution/dispensing means that can separately supply the diluted standard solution and aspirate the diluted standard solution in the standard solution storage tank for each predetermined analysis cycle and directly supply it to the biochemical analysis section; and (e) the concentration and biochemistry of the diluted standard solution. It is equipped with a calculation unit that calculates the concentration of the test component in each sample based on the detection output when it is supplied to the analysis unit, and an undiluted standard solution is introduced in place of the sample into the sample container, allowing for mobile dilution and dispensing. Based on the detection output A when supplied to the biochemical analysis department through the dilution and dispensing operation using the method, the concentration of the diluted standard solution, which is the basis for calculation, can be calculated using the following formula for each item: (I
) C=Co・A/B...(I) [In the formula, C is the concentration for calculation of the corrected diluted standard solution, Co
represents the actual concentration of the diluted standard solution, and B represents the detection output of the diluted standard solution.