JPS6317436B2 - - Google Patents
Info
- Publication number
- JPS6317436B2 JPS6317436B2 JP54038438A JP3843879A JPS6317436B2 JP S6317436 B2 JPS6317436 B2 JP S6317436B2 JP 54038438 A JP54038438 A JP 54038438A JP 3843879 A JP3843879 A JP 3843879A JP S6317436 B2 JPS6317436 B2 JP S6317436B2
- Authority
- JP
- Japan
- Prior art keywords
- deoxyribonuclease
- dna
- sepharose
- extract
- activity
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
- 102000016911 Deoxyribonucleases Human genes 0.000 claims description 40
- 108010053770 Deoxyribonucleases Proteins 0.000 claims description 40
- 238000000034 method Methods 0.000 claims description 16
- 239000000284 extract Substances 0.000 claims description 14
- 102000053602 DNA Human genes 0.000 claims description 7
- 108020004414 DNA Proteins 0.000 claims description 7
- 239000000243 solution Substances 0.000 claims description 7
- 239000007979 citrate buffer Substances 0.000 claims description 6
- 238000001179 sorption measurement Methods 0.000 claims description 6
- 229920000936 Agarose Polymers 0.000 claims description 2
- 230000002378 acidificating effect Effects 0.000 claims description 2
- 229920002684 Sepharose Polymers 0.000 description 22
- WCUXLLCKKVVCTQ-UHFFFAOYSA-M Potassium chloride Chemical compound [Cl-].[K+] WCUXLLCKKVVCTQ-UHFFFAOYSA-M 0.000 description 12
- 230000000694 effects Effects 0.000 description 11
- 102000004142 Trypsin Human genes 0.000 description 9
- 108090000631 Trypsin Proteins 0.000 description 9
- 239000012588 trypsin Substances 0.000 description 9
- 238000010828 elution Methods 0.000 description 8
- 235000011164 potassium chloride Nutrition 0.000 description 6
- 239000001103 potassium chloride Substances 0.000 description 6
- 238000000746 purification Methods 0.000 description 6
- 102000006382 Ribonucleases Human genes 0.000 description 5
- 108010083644 Ribonucleases Proteins 0.000 description 5
- 235000018102 proteins Nutrition 0.000 description 4
- 102000004169 proteins and genes Human genes 0.000 description 4
- 108090000623 proteins and genes Proteins 0.000 description 4
- 238000005406 washing Methods 0.000 description 4
- 238000002835 absorbance Methods 0.000 description 3
- 238000001042 affinity chromatography Methods 0.000 description 3
- 239000000872 buffer Substances 0.000 description 3
- 210000000496 pancreas Anatomy 0.000 description 3
- 229940062190 pancreas extract Drugs 0.000 description 3
- 102000004190 Enzymes Human genes 0.000 description 2
- 108090000790 Enzymes Proteins 0.000 description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- FKMJXALNHKIDOD-LBPRGKRZSA-N TAMe Chemical compound NC(=N)NCCC[C@@H](C(=O)OC)NS(=O)(=O)C1=CC=C(C)C=C1 FKMJXALNHKIDOD-LBPRGKRZSA-N 0.000 description 2
- QTBSBXVTEAMEQO-UHFFFAOYSA-N acetic acid Substances CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 2
- 239000007853 buffer solution Substances 0.000 description 2
- 239000000356 contaminant Substances 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 238000001914 filtration Methods 0.000 description 2
- 229950010604 pancreas powder Drugs 0.000 description 2
- 229920002477 rna polymer Polymers 0.000 description 2
- 239000012266 salt solution Substances 0.000 description 2
- 239000000758 substrate Substances 0.000 description 2
- 239000006228 supernatant Substances 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- VZSRBBMJRBPUNF-UHFFFAOYSA-N 2-(2,3-dihydro-1H-inden-2-ylamino)-N-[3-oxo-3-(2,4,6,7-tetrahydrotriazolo[4,5-c]pyridin-5-yl)propyl]pyrimidine-5-carboxamide Chemical compound C1C(CC2=CC=CC=C12)NC1=NC=C(C=N1)C(=O)NCCC(N1CC2=C(CC1)NN=N2)=O VZSRBBMJRBPUNF-UHFFFAOYSA-N 0.000 description 1
- ODKSFYDXXFIFQN-BYPYZUCNSA-N L-arginine Chemical compound OC(=O)[C@@H](N)CCCN=C(N)N ODKSFYDXXFIFQN-BYPYZUCNSA-N 0.000 description 1
- 229930064664 L-arginine Natural products 0.000 description 1
- 235000014852 L-arginine Nutrition 0.000 description 1
- 102000035195 Peptidases Human genes 0.000 description 1
- 108091005804 Peptidases Proteins 0.000 description 1
- 239000008351 acetate buffer Substances 0.000 description 1
- 239000003463 adsorbent Substances 0.000 description 1
- 230000003139 buffering effect Effects 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 238000004587 chromatography analysis Methods 0.000 description 1
- 238000000354 decomposition reaction Methods 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 238000003028 enzyme activity measurement method Methods 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 238000004255 ion exchange chromatography Methods 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 102000039446 nucleic acids Human genes 0.000 description 1
- 108020004707 nucleic acids Proteins 0.000 description 1
- 150000007523 nucleic acids Chemical class 0.000 description 1
- 235000015277 pork Nutrition 0.000 description 1
- 239000002244 precipitate Substances 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
Landscapes
- Enzymes And Modification Thereof (AREA)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP3843879A JPS55131389A (en) | 1979-04-02 | 1979-04-02 | Purification of deoxyribonuclease i |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP3843879A JPS55131389A (en) | 1979-04-02 | 1979-04-02 | Purification of deoxyribonuclease i |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS55131389A JPS55131389A (en) | 1980-10-13 |
| JPS6317436B2 true JPS6317436B2 (enrdf_load_stackoverflow) | 1988-04-13 |
Family
ID=12525299
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP3843879A Granted JPS55131389A (en) | 1979-04-02 | 1979-04-02 | Purification of deoxyribonuclease i |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS55131389A (enrdf_load_stackoverflow) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| ATE176924T1 (de) * | 1988-12-23 | 1999-03-15 | Genentech Inc | Verfahren zur herstellung von menschlicher dnase |
-
1979
- 1979-04-02 JP JP3843879A patent/JPS55131389A/ja active Granted
Also Published As
| Publication number | Publication date |
|---|---|
| JPS55131389A (en) | 1980-10-13 |
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