JPS6314640A - Culture of mushroom(enokidake) - Google Patents

Abstract

(57) [Summary] This bulletin contains application data before electronic filing, so abstract data is not recorded.

Description

DETAILED DESCRIPTION OF THE INVENTION Technical Field of the Invention The present invention relates to a method for cultivating enoki mushrooms, and more particularly to a method for cultivating enoki mushrooms that promotes the growth of enoki mushrooms and suppresses the proliferation of various bacteria. .

The technological success of the invention and its development In recent years, enoki mushrooms have been cultivated artificially.
It spreads over the entire meal. This enoki mushroom is
In general, one solid medium prepared by adding nutrients such as rice sugar and wheat flour to Koyoi is inoculated with 1 Koenoki mushroom fungus. Grow the mycelium in 1 to 7 increments, remove the enoki fungi inoculated onto the surface of the solid medium, and then sprout the fruiting bodies in a dark place at 12-15°C. After that, it has been cultured by growing in a cool, dark place at 2 to 6°C.

However, according to the method of cultivating enoki mushrooms as described above,
There was a problem in that the growth of enoki mushrooms varied, and some enoki mushrooms did not fully grow, resulting in insufficient sealing of the enoki mushrooms that could be harvested.

In addition, when inoculating Enogi mushroom fungi onto a solid medium such as the one mentioned above, it is difficult to completely prevent and cover the Enogi mushroom fungi, which may inhibit the growth of Enogi mushrooms or prevent them from growing. There was a serious problem that enoki mushrooms could turn brown.

The inventor of the present invention has made extensive research to resolve the above problems at once, and has found that the above problems can be solved by spraying Shiitake mushroom mycelium extract at a specific time on a solid medium inoculated with Enoki mushroom fungus. The present invention was completed by discovering that these problems could be solved at once.

4z Conventionally, it has been disclosed in JP-A-51-18670 that the mycelium extract of basidiomycetes such as Apr. 11 mushrooms is effective in promoting the growth of green leafy plants such as soybean, tomato, and North. It was completely unknown that shiitake mushroom mycelium extract promotes the growth of enoki mushrooms and prevents the harmful effects of bacteria on the growth of enoki mushrooms.

The present invention aims to solve the above-mentioned problems associated with the prior art at once, and is capable of promoting the growth of enoki mushrooms and preventing the growth of enoki mushrooms caused by various bacteria. The purpose is to capture and provide methods for cultivating enoki mushrooms to prevent their negative effects.

Invention It! ii.Summary The method for culturing enoki mushrooms according to the present invention involves inoculating enoki mushroom fungi into a solid medium to grow mycelium, and then growing the fruiting bodies of enoki mushrooms. After that, when the fruiting body of the enoki mushroom is less than 3 cm in height, add the shiitake mycelium extract to a solid medium or enoki mushroom.
It is characterized by the fact that it is defeated by the fruiting body.

In the present invention, since the shiitake mushroom mycelium extract is sprayed on the solid medium or the fruiting bodies of enoki mushrooms as described above,
Not only is the growth promoting effect of the fruiting body recognized, but also the growth of the enoki n fruiting body is prevented from being harmed by bacteria, and the browning of the enoki n fruiting body is prevented.

Detailed explanation of the method for culturing enoki mushroom backs according to the present invention will be described below.

In the present invention, after the enoki mushroom fungus is inoculated into a solid medium and the mycelium is grown, the fruiting body of the enoki mushroom is grown, after the mycelium of the enoki mushroom back has grown in the solid medium, and This method is characterized in that the shiitake mushroom mycelium extract is sprayed on the solid medium on which the mycelium has grown or on the enoki n fruiting body grown from the solid medium at a time when the fruiting body of the enoki mushroom is 3 cm or less in height. This J: Sea urchin The present invention is characterized by spraying the shiitake mushroom mycelium extract onto a solid medium or the fruiting bodies of enoki mushrooms. A known method can be used.

First, the conventionally known methods for culturing enoki mushrooms will be explained below.
After a specific explanation, the method for culturing enoki mushrooms according to the present invention will be explained.

First, enoki mushroom fungi are inoculated onto a solid medium. As the solid medium, a medium prepared by adding nutritional components such as rice bran and bran to sawdust is usually preferably used. It is also possible to use a solid medium made by adding nutritive ingredients such as rice bran and bran to Bacas, which is the squeezed lees of sugar cane. It is preferable to sterilize such a solid medium by heating or the like before inoculating the enoki mushroom fungus.

The solid medium inoculated with the Enogi mushroom fungus in this manner is kept at a temperature of 15 to 25°C, preferably 16 to 22°C, while being humidified, and placed in a dark culture chamber for 15 to 20 minutes.
Enoki mushroom mycelium was grown in a solid medium using a polishing device for about 25 days. Ru.

Next, the enoki mushroom fungus inoculated into the solid medium is removed, and the solid medium is then heated to 10-18°C, preferably 12-15°C.
After the fruiting bodies are transferred to a dark culture chamber kept at 0°C to 10°C, the fruiting bodies are transferred to a darkened low-temperature culture room kept at a temperature of 0 to 10°C, preferably 2 to 6°C. grow.

Although the method described above is a typical conventionally known method for culturing enoki mushrooms, for example, enoki mushrooms can also be cultured using a single culture chamber instead of using a plurality of culture chambers. In addition, after sprouting the fruiting bodies of enoki mushrooms from a solid medium,
Instead of growing fruiting bodies in a low-humidity culture chamber, the fruiting bodies of enoki mushrooms can also be grown while being irradiated with light.

In the present invention, in the conventionally known method for culturing enoki mushrooms as described above, after the mycelium of enoki mushrooms has grown in a solid medium, and furthermore, the height of the fruiting bodies of enoki mushrooms is 3 cm.
At a certain time, preferably after the mycelium has grown in the solid medium and the inoculated enoki mushroom fungus has been removed from the surface of the solid medium, the height of the fruiting body of the enoki mushroom is approximately 2 cm or less, preferably 1 cm. The method is characterized in that, at a certain time, the shiitake mushroom mycelium extract is sprayed on a solid medium on which the mycelium has grown or on an enoki bamboo shoot body grown from a solid medium.

Spraying the shiitake mushroom mycelium extract, the enoki bamboo shoot body is 3cm high.
If this is done after the Enogi bamboo shoots have grown beyond m, the bulk of the Enogi bamboo shoots will become too large and the appearance will deteriorate, which is not preferable. On the other hand, if the shiitake mushroom mycelium extract is applied before the enogi mushroom mycelium grows in the solid medium, the dispersion effect of the shiitake mushroom mycelium extract will not be sufficiently observed, which is not preferable.

The shiitake mushroom mycelium extract used in the present invention is obtained by growing shiitake fungi on a solid medium based on Bacchus, and extracts various active ingredients from a solid medium containing shiitake mushroom mycelium with water or water and alcohol. This is an extract obtained by extraction with a mixed solvent of Nato and an organic solvent. This shiitake mushroom mycelium extract contains large amounts of various amino acids, vitamins, lentinol, and the like.

It has been confirmed that C in shiitake mushroom mycelium extract contains many more nutritional and medicinal components than shiitake mushroom extract.

For example, in Shiitake mushroom mycelium, 2.07 in 1009 mycelium.
Contains threonine, an essential amino acid in ff, while in the fruiting body of the vertebrate, 0.8's per 100g of the fruiting body.
It only contains threonine.

This shiitake mushroom mycelium extract can be obtained by inoculating Shiitake fungi into a solid medium based on Bacas and rice bran, and then unbundling the solid medium containing the mycelium obtained by multiplying the mycelium.
After adding water and an enzyme such as cellulase, protease or glucidase as necessary, the solid medium is crushed and crushed to extract the active ingredient,
It is then heated to a temperature of up to 95°C to inactivate the enzyme and sterilize it.

The time to unravel the solid medium containing mycelium is preferably when the mycelium has grown sufficiently in the solid medium and immediately before or immediately after the fruiting body has appeared.

In the present invention, the shiitake mushroom mycelium extract obtained as described above may be used as is, but it is usually preferable to use it after diluting it with water.

When Shiitake mycelium extract is sprayed on a solid medium on which enoki mushrooms have grown or on enoki bamboo shoot bodies with a height of 3 cm or less that have grown from a solid medium, does the growth of enoki bamboo shoot bodies be promoted? When expressed in terms of the weight of the harvested Enogi bamboo shoots, a growth promoting effect of up to 20-40% is observed.

Furthermore, when the shiitake mycelium extract is sprayed on a solid medium or enoki bamboo shoot bodies, the growth of the enoki bamboo shoot bodies is prevented from being inhibited by contaminants that are mixed in when the solid medium is inoculated with enoki mushroom fungi. This is presumably because the shiitake mushroom mycelium extract has excellent antibacterial activity. For example, Shiitake mushroom mycelium extract contains Staphylococcus 3T, a
It has excellent antibacterial activity against viruses such as LJS.

An example of the antibacterial activity of this shiitake mushroom mycelium extract against fungi is shown in Table 1 in comparison with methyl paraben, which is known as an antibacterial agent. Since fik is applied to the fruit, it is suspected that it has a growth promoting effect on the enoki bamboo fruit, and that the growth of the enoki bamboo fruit may be inhibited by bacteria, or that the enoki bamboo fruit may turn brown.

EXAMPLES The present invention will be explained below with reference to Examples, but the present invention is not limited to these Examples.

A solid medium consisting of 80% by weight of sawdust and 20% by weight of rice bran was placed in a pot, sterilized by a conventional method, and then the enoki mushroom fungus was inoculated into the solid medium.

While humidifying the solid medium inoculated with enoki mushroom fungi,
Mycelium was grown in a solid medium in a culture chamber maintained at 16-20° C. for 18 days. Approximately 150 pots were prepared and enoki thatch was cultured.

Next, after removing the enoki mushroom fungus inoculated onto the surface of the solid medium, fruiting bodies were allowed to sprout at a temperature of 12 to 15°C.

When the length of the enoki bamboo shoot body reached approximately 0.5 mm, Shiitake mycelium extract diluted with water was sprayed onto the fruit body.

Next, this solid medium was placed in a culture chamber maintained at a humidity of 2 to 6°C, and fruiting bodies were grown. The weight of the harvested enoki mushrooms was measured and the average value of about 150 enoki mushrooms was calculated.

Table 2 shows the average harvest weight on each harvest day.

Table 2 Moreover, no browning was observed in the obtained enoki mushrooms.

Fruit cultivation 1-2 The operation of Example 1 was repeated in exactly the same manner to carry out a series of culturing of enoki mushrooms.

Table 3 shows each harvest amount and average harvest weight.

Table 3 No browning was observed in the obtained enoki mushrooms.

几11TSUJI-1 Enoki mushrooms were cultured in exactly the same manner as in Example 1, except that the shiitake mycelium extract was not sprayed.

Table 4 shows the respective yields and yields in this control plot.

Table 4 Browning was observed in some of the obtained enokiwi.

Comparative Example 2 Enoki mushrooms were cultured in exactly the same manner as in Example 1, except that the shiitake mycelium extract was sprayed after the enoki bamboo shoot bodies had grown to about 4 cm.

The resulting enoki mushrooms had a large bulk, had a poor appearance, and decreased their value as a product.

Claims (1)

[Claims] 1) After the enoki mushroom fungus is inoculated onto a solid medium and the mycelium is grown, when growing the enoki mushroom fruiting body, after the enoki mushroom mycelium has grown and the enoki mushroom fruiting body is high. A method for cultivating enoki mushrooms, which comprises spraying Shiitake fungus striae extract on a solid medium or on enoki mushroom fruiting bodies when the mushroom size is 3 cm or less.