JPS63137675A - 新規ｎａｄ（ｐ）ｈオキシダ−ゼ及びその用途 - Google Patents
新規ｎａｄ（ｐ）ｈオキシダ−ゼ及びその用途Info
- Publication number
- JPS63137675A JPS63137675A JP61283240A JP28324086A JPS63137675A JP S63137675 A JPS63137675 A JP S63137675A JP 61283240 A JP61283240 A JP 61283240A JP 28324086 A JP28324086 A JP 28324086A JP S63137675 A JPS63137675 A JP S63137675A
- Authority
- JP
- Japan
- Prior art keywords
- nad
- oxidase
- substrate
- enzyme
- nadh
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 108010002998 NADPH Oxidases Proteins 0.000 title claims abstract description 19
- 102000004722 NADPH Oxidases Human genes 0.000 title claims abstract description 19
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- 108090000790 Enzymes Proteins 0.000 claims abstract description 42
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- 239000000758 substrate Substances 0.000 claims abstract description 28
- 238000006243 chemical reaction Methods 0.000 claims abstract description 19
- 239000007788 liquid Substances 0.000 claims abstract description 18
- 230000002255 enzymatic effect Effects 0.000 claims abstract description 8
- 239000000126 substance Substances 0.000 claims abstract description 5
- 229930027945 nicotinamide-adenine dinucleotide Natural products 0.000 claims description 46
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- XJLXINKUBYWONI-DQQFMEOOSA-N [[(2r,3r,4r,5r)-5-(6-aminopurin-9-yl)-3-hydroxy-4-phosphonooxyoxolan-2-yl]methoxy-hydroxyphosphoryl] [(2s,3r,4s,5s)-5-(3-carbamoylpyridin-1-ium-1-yl)-3,4-dihydroxyoxolan-2-yl]methyl phosphate Chemical compound NC(=O)C1=CC=C[N+]([C@@H]2[C@H]([C@@H](O)[C@H](COP([O-])(=O)OP(O)(=O)OC[C@@H]3[C@H]([C@@H](OP(O)(O)=O)[C@@H](O3)N3C4=NC=NC(N)=C4N=C3)O)O2)O)=C1 XJLXINKUBYWONI-DQQFMEOOSA-N 0.000 claims description 36
- 230000000694 effects Effects 0.000 claims description 31
- 238000000034 method Methods 0.000 claims description 28
- BOPGDPNILDQYTO-NNYOXOHSSA-N nicotinamide-adenine dinucleotide Chemical compound C1=CCC(C(=O)N)=CN1[C@H]1[C@H](O)[C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OC[C@@H]2[C@H]([C@@H](O)[C@@H](O2)N2C3=NC=NC(N)=C3N=C2)O)O1 BOPGDPNILDQYTO-NNYOXOHSSA-N 0.000 claims description 27
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- 238000012360 testing method Methods 0.000 claims description 9
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- BAWFJGJZGIEFAR-NNYOXOHSSA-N NAD zwitterion Chemical compound NC(=O)C1=CC=C[N+]([C@H]2[C@@H]([C@H](O)[C@@H](COP([O-])(=O)OP(O)(=O)OC[C@@H]3[C@H]([C@@H](O)[C@@H](O3)N3C4=NC=NC(N)=C4N=C3)O)O2)O)=C1 BAWFJGJZGIEFAR-NNYOXOHSSA-N 0.000 claims 1
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- 239000003960 organic solvent Substances 0.000 description 1
- KHPXUQMNIQBQEV-UHFFFAOYSA-N oxaloacetic acid Chemical compound OC(=O)CC(=O)C(O)=O KHPXUQMNIQBQEV-UHFFFAOYSA-N 0.000 description 1
- 238000006395 oxidase reaction Methods 0.000 description 1
- 230000036284 oxygen consumption Effects 0.000 description 1
- QWUFXJHVFNRNCU-UHFFFAOYSA-N phenazin-1-amine Chemical compound C1=CC=C2N=C3C(N)=CC=CC3=NC2=C1 QWUFXJHVFNRNCU-UHFFFAOYSA-N 0.000 description 1
- 150000002989 phenols Chemical class 0.000 description 1
- 235000021317 phosphate Nutrition 0.000 description 1
- 150000003013 phosphoric acid derivatives Chemical class 0.000 description 1
- 229920002401 polyacrylamide Polymers 0.000 description 1
- 239000002244 precipitate Substances 0.000 description 1
- 238000001556 precipitation Methods 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 229940076788 pyruvate Drugs 0.000 description 1
- 229940107700 pyruvic acid Drugs 0.000 description 1
- 230000035484 reaction time Effects 0.000 description 1
- 230000009257 reactivity Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 230000002441 reversible effect Effects 0.000 description 1
- 238000005185 salting out Methods 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 238000010206 sensitivity analysis Methods 0.000 description 1
- 229910052708 sodium Inorganic materials 0.000 description 1
- 239000001540 sodium lactate Substances 0.000 description 1
- 229940005581 sodium lactate Drugs 0.000 description 1
- 239000000600 sorbitol Substances 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 239000005720 sucrose Substances 0.000 description 1
- 229910052717 sulfur Inorganic materials 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 125000003831 tetrazolyl group Chemical group 0.000 description 1
- 238000009210 therapy by ultrasound Methods 0.000 description 1
- 238000002525 ultrasonication Methods 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 230000002747 voluntary effect Effects 0.000 description 1
- 239000012138 yeast extract Substances 0.000 description 1
Landscapes
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
Priority Applications (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP61283240A JPS63137675A (ja) | 1986-11-28 | 1986-11-28 | 新規ｎａｄ（ｐ）ｈオキシダ−ゼ及びその用途 |
| DE3725851A DE3725851A1 (de) | 1986-08-12 | 1987-08-04 | Nad(p)h-oxidase, verfahren zu ihrer isolierung und anwendung |
| US07/083,921 US4954445A (en) | 1986-08-12 | 1987-08-11 | Novel NAD(P)H oxidase |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP61283240A JPS63137675A (ja) | 1986-11-28 | 1986-11-28 | 新規ｎａｄ（ｐ）ｈオキシダ−ゼ及びその用途 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS63137675A true JPS63137675A (ja) | 1988-06-09 |
| JPH0441596B2 JPH0441596B2 (enrdf_load_stackoverflow) | 1992-07-08 |
Family
ID=17662905
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP61283240A Granted JPS63137675A (ja) | 1986-08-12 | 1986-11-28 | 新規ｎａｄ（ｐ）ｈオキシダ−ゼ及びその用途 |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS63137675A (enrdf_load_stackoverflow) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2008092832A (ja) * | 2006-10-10 | 2008-04-24 | Keio Gijuku | 新規過酸化水素生成型ｎａｄｈオキシダーゼ |
-
1986
- 1986-11-28 JP JP61283240A patent/JPS63137675A/ja active Granted
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2008092832A (ja) * | 2006-10-10 | 2008-04-24 | Keio Gijuku | 新規過酸化水素生成型ｎａｄｈオキシダーゼ |
Also Published As
| Publication number | Publication date |
|---|---|
| JPH0441596B2 (enrdf_load_stackoverflow) | 1992-07-08 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| LAPS | Cancellation because of no payment of annual fees |