JPS63116653A - Feed - Google Patents

Abstract

PURPOSE:To obtain the aimed feed to enhance color tone of hen's vitellus, body color of goldfish, etc., effectively, by specifying a weight ratio of alpha-carotene and beta-carotene and a weight ratio of trans-form and cis-form of carotene. CONSTITUTION:A feed is blended with carotene (preferably palm carotene concentrated from palm oil) having a weight ratio of alpha-carotene and beta-carotene of 25-45:75-55 and a weight ratio of transform and cis-form of 60-80:40-20 to give the aimed feed.

Description

[Detailed description of the invention] The present invention relates to feed for chickens, fish, etc., and more specifically, it enhances the color of chicken egg yolk, the body color of goldfish, Nishikigoi, shrimp, etc. Concerning feed that can be.

Don't be fooled - IL/i' must be resolved -) - ζ problem solving - Conventionally, color enhancers have been added to feed in order to enhance the color of egg yolks and the body color of goldfish, etc. However, as color enhancers, natural products include paprika (cabusanthin) and spirulina, and synthetic products include canthaxanthin and β.
-Apo-8'carotic acid ethyl ester is mainly used.

In this case, synthetic products are used to enhance the yellow color for laying hens, but poultry farmers are preferring natural products. However, the current situation is that natural products such as paprika and spirulina cannot be completely replaced by synthetic products in terms of cost, quantity, and effectiveness.

Furthermore, as an emergency color tone enhancer, synthetic β-carotene (all l-lance form) has almost no effect.

Therefore, a color enhancer that is effective in terms of cost, quantity, and effectiveness is further desired.

The present invention was made in view of the circumstances described in item 1-1, and an object of the present invention is to provide a feed that can effectively enhance the color of egg yolk and the body color of goldfish.

-Uα-Rise-up-Solution-Ene marriage two sides-□I×□ノ#, Noroto (7) As a result of intensive consideration to achieve the important goals,
The ratio of α-carotene to β-carotene is 25~25 by weight
Carotene which is 45 near 5-55 and whose weight ratio of trans isomer to cis isomer is 60-80:4-0-20 is found in chicken (layer).

It was found that it is very effective as a color enhancer for fish (goldfish, Nishikigoi, shrimp, etc.) and fish (goldfish, Nishikigoi, shrimp, etc.).

That is, the above-mentioned carotene can be obtained by concentrating palm oil, for example, but when feeding chickens, fish, etc. with dark carotene added to feed, it improves the color tone of chicken eggs, chicken, fish, etc. It was also found that body color can be enhanced almost independently of the species of fish using Enhancement 5 pi. In particular, bar 7, the oil contains carotene, but the carotene (palm carotene) obtained by concentrating palm oil as mentioned above, especially the carotene obtained by alcoholysing palm oil with lower monoalcohols. A hydrophilic solvent and water are mixed with the oil phase mainly composed of lower alkyl esters of palm oil to obtain a carotene concentrate (Japanese Unexamined Patent Publication No. 61-115062), palm oil containing carotene, or The lower alkyl costyl of palm oil is brought into contact with a sgrain-divinylbenzene copolymer resin to adsorb it to the resin, and at the same time, carotene is adsorbed, and then alcohol is brought into contact with the resin to form Bar A oil sol. After elution and separation of the lower alkyl esters of the oil 11, the resin was contacted with a hydrophobic solvent 12,- of which was easily removed under a force of 1-1'J'.
Carotene concentrate (Unexamined Japanese Patent Publication No. 6112657) is a carotene concentrate containing 0.β-carotene and 1 to silane bodies and cis bodies in the above-mentioned ratios. 1 Chin is concentrated to 3000 ppm - 25% J0'', but these carotene concentrates are further mixed with balm oil, 1. Palm hi lower argyl ester base 2, and γ. - carotene, lycobin,
It contains carotenoids such as xan 1-tonoi, tocopherol, squalene, phospholipids, etc., and these ingredients are said to be useful as animal feed ingredients.
1:l-ml)] The antioxidant effect of the roll stabilizes the strength of the product (the color tone enhancer is required to have a stability of 33 months or more for the product and 17, but it is not included in the product). It is very stable due to the presence of tocopherols in it), has a good absorption effect, and since rodin is usually dissolved in par-11 oil or lower alkyl ester of palm oil, it can be added to feed uniformly. Therefore, compared to conventional natural and synthetic color enhancers, it is more effective, lower cost and stable production (1.
It is effective in terms of: 1. It is a natural product; We have discovered that it is possible to meet the demands of consumers, and have come up with the present invention.

The present invention will be explained in more detail below.

The feed according to the present invention is for chickens (layers, broilers),
It is used as feed for fish (goldfish, Nishikigoi, shrimp, sea bream, etc.), as well as for coloring the feathers of red canaries, for cattle, and as bed food for dogs and 1111. It contains α-carotene and β-carotene. The ratio of 1-lance isomer to cis isomer is 25 to 4.5875 to 55 by weight, and the ratio of 1-lance isomer to cis isomer is 60 by weight.
-80:40-.20 carotene is added.

There is no limit to the method of obtaining the carotene mentioned above, and 0.

-) is obtained by mixing the β-form, trans-form, and cis-form within the above range. Although it is possible to use palm carotene, it is particularly preferable to use one concentrated from palm carotene (palm carotene). In this case, the lower alkyl ester of palm oil obtained by alcoholysing palm oil with a lower monoalcohol such as methanol or ethanol is used as the concentration method from par 11 oil. A method of precipitating and separating carotene by adding water and a hydrophilic solvent such as meth, ethanol, or ethanol to the oil phase is preferably adopted.

In addition, by contacting the lower argyl seaester (oily substance) of balm oil with the stylinone-divinylbenzene copolymer resin, the stylinone-divinylbenzene copolymer resin has a force of 11 tin and an oily substance. The substance was adsorbed, and then the above-mentioned styrene divinylbenzene copolymer resin was contacted with the lower divinylbenzene copolymer resin. After separating the oil-distributing substance from the alcohol by elution I, the styrene-divinylbenzene copolymer resin was mixed with hexane,
Method IJ is preferably employed in which a hydrophobic solvent in which carotene is readily soluble, such as chloroform or petroleum ether, is brought into contact and agitated, and the carotene is eluted and separated in this hydrophobic solvent.

The carotene a-condensate obtained in this manner contains, for example, 0.3 to 25% of total rotinoid (weight %, same hereinafter), 0.01 to 0.7% of xanthophyll, and 0.7% of tocopherol.
Contains 05-0.1%, sterol ('), 04-1%, phospholipid 0.02-1%, squalene 0.1-5%, fatty acid alkyl ester 99-60%, etc., making it a total rotinoid. It contains 0.1-8% of α-carotene, 0.2-15% of β-carotene, 0.02-1.6% of other carotenoids (γ-carotene, lycopene), and
-Hydrotin, β-carotene 25-4-5: 75-
55, I-lance form, cis form 60-80: 4.0
20%, and therefore has an excellent effect as a color tone enhancer.

These carotene concentrates are based on lower alkyl esters of palm oil or par-11 oil and usually have the carotene dissolved therein, so when added to feed, they can be sprayed into the feed base.

Added? I'! A four-match method can be adopted. It is also possible to mix powder instead of adding liquid by microcapsulating it with gelatin or the like or spray drying it after emulsification.

The feed of the present invention is made by adding carotene to the feed as described above, and the ratio of α-carotene to β-carotene is 25 to 45 (nearly 5 to 55) by weight, and 1 - The ratio of lance isomer to cis isomer is 60 to 80 by weight
: 4. The amount of carotene to be added to feed, which is 0 to 20, is selected depending on the type of feed, etc., but 0.00
It is preferably 0.05 to 0.1%, particularly 0.001 to 0.01%.

In addition,! As the feed base to which oily carotene is added, known feed bases can be used depending on the type of animal.

According to the feed of the present invention, by adding a specific carotene, it is possible to enhance the color tone of the egg yolk as a feed for laying hens (for example, the yoke of the egg yolk). It has a good effect on color tone enhancement, such as increasing the color fan value from 5 to 8), and has a color-increasing effect on goldfish, Nishikigoi, crab shrimp, etc. In addition to being used for broilers, for coloring the feathers of red canaries, for sea breams, and for promoting pregnancy in chickens and cows, it is also suitably used for a variety of purposes, including as a bed food to improve the coat quality of dogs and cats.

EXAMPLES Hereinafter, the present invention will be specifically explained with reference to Examples.

[Example 1] As egg-laying hens, we used individuals of the White Leghorn breed that were 280 days old and 4 months old after starting to lay eggs (10 birds per piece).
After confirming that the egg production rate was 75% or more on average, we conducted a test to enhance the color in the egg yolk. As a preliminary rearing period, for 10 days, low carotenoid basal diet (total rotinoid 4.2 ppm) and high carotenoid diet were used. After growing separately in the basal feed (total rotinoid 17.4 ppm) group, gelatin capsules (average particle size 0.3 mmφ) of the color tone enhancer of the present invention were added to the carotene concentration of 5 pp.
Q-M, 1.0 ppm, 20 ppm was mixed with the Q-basal feed and continuously administered by ad libitum feeding for 20 days. No. 15).

The results are shown in Table 2.

In addition, - membrane properties, etc., egg production rate, egg Hau unit, egg weight, and yellow tint coefficient were checked, but no effects were observed at all due to the addition of the color tone enhancer.

Here, the composition of the basic feed for laying hens is as shown in Table 1.

In addition, as the gelatin capsule (color tone enhancer of the present invention), carotene (7%
, 7% lower alkyl ester solution of carotene) was dispersed in the core, 20% aqueous sodium sulfate was added dropwise for phase separation, and then ]-0
Cool to ℃, after gelation, raise to room temperature -15%
The product was washed with sodium sulfate, dehydrated and filtered, and air-dried.

Gelatin 35% Lower alkyl ester 60% Carotene 5% From the results in Table 1 and Table 2, it was confirmed that the color tone of egg yolk was enhanced by the addition of carotene. .

[Example 2] For the test goldfish, choose one with a single red color and pay interest.

A body color enhancement test was conducted on 7 mice in each test group of sulfuric acid and comet.

The test plot consisted of basal feed (total rotinoid 2, 8
a group fed only 1,100 ppm of the color enhancer of the present invention as carotene to the same basic feed, and a group fed the same basic feed with 1000 ppm of the color enhancer of the present invention added as carotene. The goldfish were divided into groups and feed was intermittently fed at an amount of 3 to 4% of the goldfish body weight per day, divided into three times: morning, afternoon, and night. Body color was observed for about 2 months and evaluated based on the average body color of 7 mice in each test group.

As a result, there was almost no change in body color in the group receiving only basal feed, but in the group supplemented with 1.0 Qppm of carotene,
IS color chart: 1.0 R6/1. , 4 to 7.5R6
/12, 1. l0 in the OOOppm addition area
The color tone became clearer from R6/'4 to 7 and 5 R5/i4, and from this it was confirmed that the body color of goldfish could be enhanced by the addition of carotene.

In addition, when growing goldfish, the growth of algae and moss was suppressed and external factors were eliminated.

Furthermore, the color tone enhancer of the present invention has a DM of about 50% after pulverizing it for emergency feed. Add water to L sea urchin,
A carotene solution (lower alkyl ester solution containing 7% carotene) was kneaded therein in a mortar, kneaded in a granulator, shaped into rods in a bloater, and air-dried.

Furthermore, the composition of the basal feed is as follows.

Animal raw materials (fish meal, feather meal, fish solubles): 48-73% Grains (wheat flour, barley, soybean flour): 25-35
% Vegetable oil meal (soybean oil meal, corn gluten meal) =
5-15% Rice bran (rice bran oil cake): 2-9%
Other (fermentation) υ9 salt, oil and fat) = 2 to 7% [Example 3] Low carotenoid basic feed for laying hens (total carotenoid 4
．． 2 ppm) to which the color tone enhancer of the present invention was added as carotene at 10 ppm and 20 ppm at 35°C.

After storing for 3 months in an atmosphere with a humidity of 85%, the total amount of rotinoid was measured and the residual rate was determined. 3rd result
Shown in the table.

The color tone enhancer and basic feed of the present invention are the same as in Example 1.

Table 3 [Example 4] A test was conducted using 20 shrimp in each test group with a size of 4.4 to 4.8 g/fish (n=2).

In this case, the feed and color enhancer are the same as in Example 2,
Feed intermittently for about 2 months so that there is no leftover food, and 1.
A comparison was made between the 000 ppm addition group and the blank.

As a result, there was no difference in the external color tone (blue) when alive, but by heating and boiling, one blank had an IS standard color chart of 2.5YR, 5/10, while one dead ~
There was a clear difference between ward 7 and 5 R5/14.

Claims (1)

[Claims] 1. The ratio of α-carotene to β-carotene is 2 by weight.
5 to 45:75 to 55, and the ratio of trans isomer to cis isomer is 60 to 80:40 to 20 by weight. 2. The feed according to claim 1, wherein the carotene is palm carotene concentrated from palm oil.