JPS6248398A - 化学発光法による酵素の定量法 - Google Patents
化学発光法による酵素の定量法Info
- Publication number
- JPS6248398A JPS6248398A JP18581785A JP18581785A JPS6248398A JP S6248398 A JPS6248398 A JP S6248398A JP 18581785 A JP18581785 A JP 18581785A JP 18581785 A JP18581785 A JP 18581785A JP S6248398 A JPS6248398 A JP S6248398A
- Authority
- JP
- Japan
- Prior art keywords
- fluorescent substance
- enzyme
- reaction
- liberate
- added
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 102000004190 Enzymes Human genes 0.000 title claims abstract description 26
- 108090000790 Enzymes Proteins 0.000 title claims abstract description 26
- 238000000034 method Methods 0.000 title claims description 22
- 239000000126 substance Substances 0.000 claims abstract description 23
- MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 claims abstract description 22
- 238000006243 chemical reaction Methods 0.000 claims abstract description 18
- -1 4-methylumbelliferyl galactoside Chemical class 0.000 claims abstract description 10
- 150000001875 compounds Chemical class 0.000 claims abstract description 4
- MUBZPKHOEPUJKR-UHFFFAOYSA-N Oxalic acid Natural products OC(=O)C(O)=O MUBZPKHOEPUJKR-UHFFFAOYSA-N 0.000 claims description 18
- 235000006408 oxalic acid Nutrition 0.000 claims description 8
- 238000004445 quantitative analysis Methods 0.000 claims description 3
- CBZOGAWUNMFXFQ-UHFFFAOYSA-N bis(2,4-dinitrophenyl) oxalate Chemical compound [O-][N+](=O)C1=CC([N+](=O)[O-])=CC=C1OC(=O)C(=O)OC1=CC=C([N+]([O-])=O)C=C1[N+]([O-])=O CBZOGAWUNMFXFQ-UHFFFAOYSA-N 0.000 claims description 2
- GEVPIWPYWJZSPR-UHFFFAOYSA-N tcpo Chemical group ClC1=CC(Cl)=CC(Cl)=C1OC(=O)C(=O)OC1=C(Cl)C=C(Cl)C=C1Cl GEVPIWPYWJZSPR-UHFFFAOYSA-N 0.000 claims 1
- 230000035945 sensitivity Effects 0.000 abstract description 7
- 239000003960 organic solvent Substances 0.000 abstract description 2
- 150000005690 diesters Chemical class 0.000 abstract 2
- 239000003054 catalyst Substances 0.000 abstract 1
- 230000002255 enzymatic effect Effects 0.000 abstract 1
- 229940088598 enzyme Drugs 0.000 description 19
- 239000003153 chemical reaction reagent Substances 0.000 description 18
- 238000004020 luminiscence type Methods 0.000 description 15
- 239000000758 substrate Substances 0.000 description 13
- 238000004458 analytical method Methods 0.000 description 10
- 239000000523 sample Substances 0.000 description 8
- WEVYAHXRMPXWCK-UHFFFAOYSA-N Acetonitrile Chemical compound CC#N WEVYAHXRMPXWCK-UHFFFAOYSA-N 0.000 description 6
- XEKOWRVHYACXOJ-UHFFFAOYSA-N Ethyl acetate Chemical compound CCOC(C)=O XEKOWRVHYACXOJ-UHFFFAOYSA-N 0.000 description 6
- 238000002795 fluorescence method Methods 0.000 description 6
- 238000005259 measurement Methods 0.000 description 6
- 239000000243 solution Substances 0.000 description 6
- 102000003914 Cholinesterases Human genes 0.000 description 5
- 108090000322 Cholinesterases Proteins 0.000 description 5
- 238000010521 absorption reaction Methods 0.000 description 5
- 229940048961 cholinesterase Drugs 0.000 description 5
- 238000002360 preparation method Methods 0.000 description 4
- 102000004882 Lipase Human genes 0.000 description 3
- 108090001060 Lipase Proteins 0.000 description 3
- 239000004367 Lipase Substances 0.000 description 3
- 239000000872 buffer Substances 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 230000003301 hydrolyzing effect Effects 0.000 description 3
- 235000019421 lipase Nutrition 0.000 description 3
- 238000011002 quantification Methods 0.000 description 3
- 239000012488 sample solution Substances 0.000 description 3
- 239000011550 stock solution Substances 0.000 description 3
- HSHNITRMYYLLCV-UHFFFAOYSA-N 4-methylumbelliferone Chemical compound C1=C(O)C=CC2=C1OC(=O)C=C2C HSHNITRMYYLLCV-UHFFFAOYSA-N 0.000 description 2
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 description 2
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 2
- 239000007864 aqueous solution Substances 0.000 description 2
- ZYGHJZDHTFUPRJ-UHFFFAOYSA-N coumarin Chemical compound C1=CC=C2OC(=O)C=CC2=C1 ZYGHJZDHTFUPRJ-UHFFFAOYSA-N 0.000 description 2
- 238000001952 enzyme assay Methods 0.000 description 2
- YQGOJNYOYNNSMM-UHFFFAOYSA-N eosin Chemical compound [Na+].OC(=O)C1=CC=CC=C1C1=C2C=C(Br)C(=O)C(Br)=C2OC2=C(Br)C(O)=C(Br)C=C21 YQGOJNYOYNNSMM-UHFFFAOYSA-N 0.000 description 2
- 150000002148 esters Chemical class 0.000 description 2
- QRMZSPFSDQBLIX-UHFFFAOYSA-N homovanillic acid Chemical compound COC1=CC(CC(O)=O)=CC=C1O QRMZSPFSDQBLIX-UHFFFAOYSA-N 0.000 description 2
- 239000008363 phosphate buffer Substances 0.000 description 2
- HSSLDCABUXLXKM-UHFFFAOYSA-N resorufin Chemical compound C1=CC(=O)C=C2OC3=CC(O)=CC=C3N=C21 HSSLDCABUXLXKM-UHFFFAOYSA-N 0.000 description 2
- RGJTZDRUTUWWTD-UHFFFAOYSA-N (7-oxophenoxazin-3-yl) butanoate Chemical compound C1=CC(=O)C=C2OC3=CC(OC(=O)CCC)=CC=C3N=C21 RGJTZDRUTUWWTD-UHFFFAOYSA-N 0.000 description 1
- FOSXAUKTMJXZDC-UHFFFAOYSA-N 2-oxo-2-(2,3,4-trichlorophenoxy)acetic acid Chemical compound OC(=O)C(=O)OC1=CC=C(Cl)C(Cl)=C1Cl FOSXAUKTMJXZDC-UHFFFAOYSA-N 0.000 description 1
- 241000589516 Pseudomonas Species 0.000 description 1
- PLXBWHJQWKZRKG-UHFFFAOYSA-N Resazurin Chemical compound C1=CC(=O)C=C2OC3=CC(O)=CC=C3[N+]([O-])=C21 PLXBWHJQWKZRKG-UHFFFAOYSA-N 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 239000000538 analytical sample Substances 0.000 description 1
- 238000000149 argon plasma sintering Methods 0.000 description 1
- NWCHELUCVWSRRS-UHFFFAOYSA-N atrolactic acid Chemical compound OC(=O)C(O)(C)C1=CC=CC=C1 NWCHELUCVWSRRS-UHFFFAOYSA-N 0.000 description 1
- 238000011088 calibration curve Methods 0.000 description 1
- 229910002092 carbon dioxide Inorganic materials 0.000 description 1
- 239000001569 carbon dioxide Substances 0.000 description 1
- 239000007795 chemical reaction product Substances 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 229960000956 coumarin Drugs 0.000 description 1
- 235000001671 coumarin Nutrition 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- MOTZDAYCYVMXPC-UHFFFAOYSA-N dodecyl hydrogen sulfate Chemical compound CCCCCCCCCCCCOS(O)(=O)=O MOTZDAYCYVMXPC-UHFFFAOYSA-N 0.000 description 1
- 229940043264 dodecyl sulfate Drugs 0.000 description 1
- 238000006911 enzymatic reaction Methods 0.000 description 1
- 230000005284 excitation Effects 0.000 description 1
- 150000008195 galaktosides Chemical class 0.000 description 1
- 239000007789 gas Substances 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 238000006460 hydrolysis reaction Methods 0.000 description 1
- OSZNNLWOYWAHSS-UHFFFAOYSA-M neostigmine methyl sulfate Chemical compound COS([O-])(=O)=O.CN(C)C(=O)OC1=CC=CC([N+](C)(C)C)=C1 OSZNNLWOYWAHSS-UHFFFAOYSA-M 0.000 description 1
- 229960002253 neostigmine methylsulfate Drugs 0.000 description 1
- BOPGDPNILDQYTO-NNYOXOHSSA-N nicotinamide-adenine dinucleotide Chemical compound C1=CCC(C(=O)N)=CN1[C@H]1[C@H](O)[C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OC[C@@H]2[C@H]([C@@H](O)[C@@H](O2)N2C3=NC=NC(N)=C3N=C2)O)O1 BOPGDPNILDQYTO-NNYOXOHSSA-N 0.000 description 1
- 229930027945 nicotinamide-adenine dinucleotide Natural products 0.000 description 1
- 150000002942 palmitic acid derivatives Chemical class 0.000 description 1
- 125000001997 phenyl group Chemical class [H]C1=C([H])C([H])=C(*)C([H])=C1[H] 0.000 description 1
- 229920001184 polypeptide Polymers 0.000 description 1
- 102000004196 processed proteins & peptides Human genes 0.000 description 1
- 108090000765 processed proteins & peptides Proteins 0.000 description 1
- 239000011541 reaction mixture Substances 0.000 description 1
- 210000002966 serum Anatomy 0.000 description 1
- 239000012086 standard solution Substances 0.000 description 1
- 239000012089 stop solution Substances 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
- UFTFJSFQGQCHQW-UHFFFAOYSA-N triformin Chemical compound O=COCC(OC=O)COC=O UFTFJSFQGQCHQW-UHFFFAOYSA-N 0.000 description 1
- 239000002023 wood Substances 0.000 description 1
Landscapes
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP18581785A JPS6248398A (ja) | 1985-08-26 | 1985-08-26 | 化学発光法による酵素の定量法 |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP18581785A JPS6248398A (ja) | 1985-08-26 | 1985-08-26 | 化学発光法による酵素の定量法 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS6248398A true JPS6248398A (ja) | 1987-03-03 |
| JPH0544280B2 JPH0544280B2 (enrdf_load_stackoverflow) | 1993-07-05 |
Family
ID=16177399
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP18581785A Granted JPS6248398A (ja) | 1985-08-26 | 1985-08-26 | 化学発光法による酵素の定量法 |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS6248398A (enrdf_load_stackoverflow) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5736320A (en) * | 1993-05-12 | 1998-04-07 | Schlederer; Thomas | Method of detecting substances by chemiluminescence |
-
1985
- 1985-08-26 JP JP18581785A patent/JPS6248398A/ja active Granted
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5736320A (en) * | 1993-05-12 | 1998-04-07 | Schlederer; Thomas | Method of detecting substances by chemiluminescence |
Also Published As
| Publication number | Publication date |
|---|---|
| JPH0544280B2 (enrdf_load_stackoverflow) | 1993-07-05 |
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