JPS62294434A - Apparatus for forming monomolecular built-up film - Google Patents

Abstract

PURPOSE:To obtain a high quality monomolecular built-up film simply and efficiently, by partitioning the monomolecular film developed to the water surface of a single developing water tank into a plurality of sections by partition barriers and transferring the partitioned films to a carrier successively. CONSTITUTION:Molecules (a) are diffused onto the water surface of a developing water tank and a moving barrier 12 is moved to form a monomolecular film. Next, a plurality of partition barriers 7 surface-treated so as to have the same property as the surface of a carrier are placed on a rising and falling arm 8 and the partition barriers are allowed to fall until the bottom surfaces thereof are contacted with the monomolecular film (a). At this time, since the bottom surfaces of the partition barriers are brought to a state of adsorbing the monomolecular film, to change is generated in the densities of the monomolecular films on the partitioned water surfaces. Next, the monomolecular films on the water surfaces of the partitioned developing water tanks S are successively transferred while a carrier 19 is moved to build up a multilayered monomolecular film on the surface of the carrier. By this method, a built-up film is obtained by simple operation without taking a process for adjusting the density of a molecule at every one transfer as is conventional.

Description

Detailed Description of the Invention [Industrial Field of Application] The present invention is applicable to a sample used for structural analysis of proteins, etc., on the surface of a solid such as glass (hereinafter referred to as a carrier). Among film formation methods that transfer the developed monomolecular film onto the surface of a liquid filled in a developing tank, the carrier is gently lowered parallel to the liquid surface, and then the carrier is pulled up to form a monolayer molecular film. The present invention relates to a film forming apparatus for depositing a monomolecular cumulative film on a carrier surface (commonly referred to as a horizontal deposition method) for forming a plurality of monomolecular films in a stacked manner on a carrier.

[Prior Art] Conventionally, as a device for accumulating a monomolecular film on a carrier between multiple layers, molecules of the type of substance to be collected are deposited on the surface of a liquid such as water or alcohol filled in a single liquid tank. After developing it to form a single layer of molecular film, immerse a carrier such as surface-treated glass parallel to the liquid surface, then pull up the carrier and transfer the single layer of monolayer film. After correcting the molecular density of the developing substance to the initial molecular density by moving the migration barrier that moves along the top of the water tank by the area occupied by the molecules transferred by the carrier, the monomolecular film has already been transferred. The carrier is immersed horizontally above the liquid level again, pulled up to accumulate a second monolayer, and the same method is repeated to form multiple layers of the same monolayer. Accumulation was being done. However, when using only the conventional single water tank mentioned above, the molecular density of the developing substance on the liquid surface changes every time the developing substance is transferred to the carrier.
The moving barrier must be moved carefully for precise calculations, and the state of the developed material must be monitored by measuring the molecular density along the way, making it difficult to deposit large cumulative layers. had the disadvantage that it was very time consuming.

[Problems to be Solved by the Invention] The present invention provides a method for forming a multi-layer monomolecular film using the conventional single development water tank, in which the moving barrier gold is moved each time a monomolecular layer is transferred to a carrier. Create multiple layers of monomolecules in a simpler process without having to go through the complicated process of layering! The purpose of this invention is to provide an apparatus capable of forming a gold film, and also to provide an apparatus that takes economic efficiency into account by making use of the conventionally used expansion tank.

[Means and effects for solving the problems] In order to solve the above-mentioned problems, the present invention uses a single expansion tank, which is the same as the conventional technology, but the liquid level of the single expansion tank is Problems can be solved by making it possible to transfer the developed substances in a partitioned development tank, which is divided into multiple partitioned development tanks using partition barriers so that the developed monolayer layer does not change the molecular density, onto the carrier in any order. This is an attempt to solve the problem.

EXAMPLES] Hereinafter, the present invention will be described in detail with reference to the drawings showing examples.

FIG. 1 is a book showing the entire apparatus of the present invention. In the figure, 1 is a developing water tank, which is provided with an inlet 2 and an outlet 3 for temperature-adjusting reflux water. Reference numeral 4 denotes a partition bar having a rectangular cross section and fitted along the inside of the longitudinal side portion 1a of the expansion water tank 1. 5 is a sink, and the expansion water tank 1 is supported by a support rod 6 protruding from the bottom of the sink. A partition barrier 7 is placed on the upper side of a pair of barrier lifting arms 8, 8. Reference numeral 9 denotes a barrier lifting arm which raises and lowers the barrier lifting arm ses parallel to the upper surface of the longitudinal side 1a of the H open water tank 1, and when turned clockwise, the barrier lifting arm 8
．． 8 is raised, and the bottom surface 7m of the partition barrier 7 is separated from the upper side 1a of the expansion water tank 1, as shown in FIG. The barrier lifting arms 8, 8 are supported by the bottom of the sink 5 by a portal-shaped support leg 10 and a lifting section 11, and the lifting mechanism is built into the portal-shaped support leg 10. Reference numeral 12 denotes a movable barrier, which is placed on the upper side 1a of the expansion tank 1, and is movable along the longitudinal direction of the expansion tank by means of a barrier transfer rod 13.

Reference numeral 14 denotes a barrier control section, which has a built-in stepping motor for moving the barrier transfer rod 13.

Reference numeral 15 denotes a base, and screws 16 for maintaining horizontality protrude from the four corners of the bottom of the base. Also, 17 is a molecular membrane pressure meter, which is suspended! By raising and lowering the rope 18,
1 The molecular membrane pressure dyn//ctn2 of the developing substance 9 on the liquid R filled in the developing water tank 1 is measured. Reference numeral 19 is a carrier such as a glass plate, which is suspended from a carrier lifting device 21 via a holder 20 horizontally with respect to the liquid level. The process of accumulating a monomolecular film using the apparatus of the present invention having the above configuration will be described below.

(1) After adjusting the horizontal maintenance screw 16 to bring the expansion tank 1 into a horizontal state, fill the expansion tank 1 with a liquid such as water or alcohol so that the liquid level is flush with the top surface of the expansion tank. . At this time, the barrier lifting arm 8.8 is
The partition barrier 7 is in the raised state shown in FIG.
As shown in the figure, it is away from the upper surface IIL of the expansion water tank 1.

(2) Diffuse the molecules a of the substance to be developed next on the water surface, and use the moving barrier 1 so that the molecules become monomolecular on the water surface.
Move 2 and apply piston pressure to adjust.

(3) Place a plurality of partition barriers 7 on the barrier lifting arm. The mutual spacing between the partition barriers 7 may be arbitrary as long as the carrier 19 can pass through, but in order to prevent movement during work, positioning holes 8& are provided in the upper surface of the barrier lifting arm 8. A positioning pin 7a is provided protruding from the side, and by fitting the two together 6, the partition barrier 7 becomes the barrier lifting arm 8.
so that they are fixed at equal intervals. The material of the partition barrier 7 is surface-treated to have the same properties (hydrophilic or hydrophobic) as the surface of the carrier.

(4) In the above state, when the barrier lift arm 8.8 is turned counterclockwise, the barrier lift arm 8.8 lowers at the same time while remaining in the horizontal position, as shown in Figs. 3 and 5. The bottom surface 7b of the partition barrier 7 contacts the upper surface 1a of the developing water tank 1 and the developed monomolecular film a on the liquid R. At this time,
Since the bottom surface 7b of the partition barrier 7 is in a state where the monomolecular film a is adsorbed, there is no change in the density of the monomolecular film on the liquid surface in the part where the bottom surface 7b of the partition barrier 7 does not contact. The monomolecular film densities on the liquid surface partitioned by 7 are all the same.

(5) The water tank 1 can be expanded by the partition barrier 7.
Since a plurality of partitioned developing tanks 18 having the same molecular density appear, while moving the carrier 19 with the carrier lifting device 21 in the longitudinal direction of the developing tank 1, the monomolecular film a of the partitioned υ developing tank 1s is sequentially moved. By transferring it to the surface 1 of the carrier 19! Several monolayer layers can be accumulated.

This state is schematically shown in the drawings from FIG. 6 onwards. That is, Fig. 6 shows the first sampling state, Fig. 7 shows the first pulling state, Fig. 8 shows the second collecting state, and Fig. 9 shows the second pulling state. By repeating this process, a monomolecular cumulative film having a desired number of layers can be formed.

Note that the function of the partition bar 4 is that when using a conventional developing water tank with a large opening surface, the amount of developing substance on the liquid 1j is large, and the liquid level is unstable if the area of the water surface is large. Unfortunately, the width of the expansion tank is narrowed to reduce the surface area of the liquid. This reduces the amount of developing material on the liquid surface and also
Since the width of the partitioned development water tank IB is narrowed from the left and right by the partition bar 4, fluctuations in the liquid level are significantly reduced and the liquid level is stabilized, making it easier to immerse the carrier 19 in the developing substance. This has the effect of greatly improving efficiency.

[Effects of the Invention] As described above, the monomolecular cumulative film forming apparatus according to the present invention does not require the conventional complicated process of adjusting the molecular density of the spread material for each deposition. It is possible to partition the monomolecular film pre-deployed on the liquid surface into multiple small partitioned development tanks without changing the molecular density while using one development tank. The same effect is produced, and for this reason, it is possible to obtain high-quality films even with a large number of stacked layers by a simple operation such as moving the carriers one after another onto a partitioned development tank and collecting them. This is a great invention.

[Brief explanation of the drawing]

The drawings all show embodiments of the present invention; Fig. 1 is a perspective view showing the entire device, Fig. 2 is a vertical cross-section of the main part along line ■-■ in Fig. 2 is a vertical sectional view of the main part showing the lowered state of the barrier lifting arm, FIG. 4 is an enlarged sectional view along the line ■-■ in FIG. 2, and FIG. ! 6, 7, 8, and 9 are explanatory diagrams schematically showing the process of forming a monomolecular cumulative film. DESCRIPTION OF SYMBOLS 1... Deployment water tank, 4... Partition bar, 7... Partition barrier, 8... Barrier elevating arm, 9... Barrier elevating arm, 12... Moving barrier, 7IL... Fixing pin, 8m...fixing hole. Applicant's agent Patent attorney Takehiko Suzue -10- Figure 3 7'' Figure 6 q Figure 7 Figure 8 Figure 9

Claims (3)

[Claims] (1) A deployment water tank having a moving barrier that applies piston pressure to the deployable substance on the liquid surface, a barrier lifting arm provided parallel to the outside of the longitudinal side of the deployment tank, and a lifting mechanism that horizontally raises and lowers the barrier lifting arm. A monomolecular cumulative film forming apparatus characterized by having: (2) The monomolecular cumulative film forming apparatus according to claim (1), wherein positioning holes for fixing the partition barrier are provided at equal intervals on the upper surface of the barrier lifting arm. (3) Claim (1) comprising a deployment water tank in which the effective area of the deployment water tank is reduced by attaching a rod-shaped partition bar with the same length as the inner diameter of the deployment tank along the inside of the longitudinal side of the deployment water tank. The monomolecular cumulative film deposition apparatus described above.