JPS6354933A - Monomolecular built-up film forming device - Google Patents

Abstract

PURPOSE:To manufacture a monomolecular built-up film composed of many layers in a short time by partitioning the liquid surface part of developing water tanks having same liquid phase to prevent the developing substance on the liquid surface from being mixed and providing a fixed barrier fitted with a freely opening and closing gate permeating only a carrier. CONSTITUTION:In case a carrier 15 is descended to the position wherein developing substance A is developed on the liquid surface 4a of a developing water tank 1A and immersed thereinto, the monomolecular membrane of substance A is shifted and stuck on the surface of the carrier 15. Then the carrier 15 is parallel moved and passed through gates 11, 12 and transferred to a developing water tank 1B side. The carrier 15 is ascended from a developing water tank 1B developed with developing substance B, and at this time, the monomolecular film of substance B is built up on the monomolecular film of substance A stuck on the carrier 15. In this method, the removing work of developing substance which was heretofore performed in a single developing water tank can be obviated and the monomolecular built-up film composing many layers can be manufactured in a short time.

Description

[Detailed Description of the Invention] [Industrial Field of Application] The present invention is applicable to a sample for structural analysis of proteins, for example, in which a sample is placed on the surface of a solid such as glass (hereinafter referred to as a carrier) and placed in a water tank. A film forming device (commonly known as Langmulr-Blodgett
In this method, two types of amphiphiles a and b are alternately collected on a carrier to form a monomolecular heterolaminar film (commonly known as an alternating cumulative LB film) with an asymmetry of 6λB...AB''. The present invention relates to a film forming apparatus for forming an asymmetrical cumulative film (referred to as a polar film).

[Prior Art] Conventionally, the method of accumulating a monomolecular film on a carrier involves depositing a monolayer of the type of substance to be formed on the surface of a liquid such as water or alcohol filled in a single liquid tank. A device that keeps the density of the molecular film on the liquid surface constant by applying piston pressure with a moving barrier to reduce the area of the liquid surface on which the developed molecular weight is floating at the same time as a carrier at a very slow speed. A monomolecular film is transferred onto a carrier by a combination of However, according to the conventional method using a single liquid bath, when the carrier is lowered into the liquid surface and the monomolecular layer is transferred to it during the process of immersion, when the carrier is lifted up as it is, the monomolecular layer from which the hydrophilic groups have already been transferred is formed. Two layers of homopolar monomolecular films arranged in the same direction as "AA" are laminated.

To prevent this, - immerse the monomolecular film of the friend substance in the carrier liquid to which it has been transferred, completely remove the human substance on the surface of the liquid, and allow the substance B to develop into a new phase. A step is taken in which a carrier is pulled up to obtain a two-layer monomolecular film.

In order to form two types of alternating monomolecular cumulative films called "AB...AB", (1) A carrier is dropped into a liquid tank in which human substances have been developed, and a monomolecular cumulative film of human substances is deposited on the carrier. Transfer to the surface.

(2) Submerge the carrier in the liquid tank 7? : Completely removes the developing substance on the liquid surface.

(3) Next, Substance B is spread on the liquid surface and the carrier is pulled up to transfer Substance B onto the human substance.

(4) Completely remove substance B on the liquid surface.

By repeating (1) to (4) below, “AB...A”
A monomolecular heterolaminar film with an asymmetric structure called "B" was formed.

However, with this method, the process of completely removing the substance spread on the liquid surface is complicated and time-consuming, and the molecules on the carrier deteriorate during long-term operations, resulting in single molecules with a large number of layers. There were limits to the formation of heterogeneous films.

[Problems to be Solved by the Invention] The present invention simplifies the work by eliminating the wasteful steps of developing the conventional monolayer and washing away the next monomolecular layer each time a single layer of monomolecular film is transferred to a carrier. The present invention aims to provide a high-quality film forming apparatus for monomolecular heterolaminar films with a greater number of layers than ever before.

[Means for Solving the Problems] In order to solve the above-mentioned problems, the present invention provides a gate that can be opened and closed at the top of the liquid tank, thereby partitioning the liquid surface in the liquid tank. The human substance is deployed on one liquid surface, and the B substance is deployed on the other liquid surface, and each time the carrier is raised and lowered, the carrier moves horizontally and is separated by a carrier partition plate. In order to prevent the 82 types of substances from mixing, a mechanism was adopted in which the carriers alternately come into contact with single molecules on the liquid surface of the two development tanks by passing through the opening and closing part of the gate or above the r-t. By doing this, the problem is solved.

[Example] The details of the apparatus of the present invention will be described below with reference to the drawings showing examples.

Figure 1 shows the entire device, and 1 in the figure is a hollow deployment tank, consisting of deployment tank IA for deploying human substances and deployment tank IB for deploying B substance, both of which are at the bottom. There are continuously an inlet 2 and an outlet 3 for temperature-adjusted reflux water in each of the development tanks, and the liquid 4 injected into the development tanks 7A and IB is maintained at a constant temperature. A deep IC is provided in the center of the deployment water tank 1, and the carrier is raised and lowered in this part to be transferred. 5A and 5B are movable barriers that are in contact with the upper edge 6 of the expansion water tank 1 and the liquid surface 1aK to apply piston pressure to the expansion substance, and are connected to a barrier transfer frame 8 that is moved in the longitudinal direction by a stepping motor 7. are doing. Reference numerals 9 and 10 are fixed barriers located in the middle of the expansion tank 1, and base portions 9a and 10h are connected to the upper edge 6 of the expansion tank 1 at a tip portion 9b.

10b are fixed so as to face each other. At the tips of the fixed barriers 9 and 10, gates 11 and 12, which can be opened and closed, are protruded from the ends of the fixed barriers 9 and 10 and made of a thin elastic hydrophobic film. This gate 11, 12d is made of a hydrophobic thin film, and the end portions 11b, 12b are fixed at the tips 9b, 10b of the fixed barrier 9° 10 so that the curved portions 11a, 12m that are elastically curved are in contact with each other with constant pressure. It is glued to the side walls 9c and 10c of.

Reference numeral 13 is an elevator, and the carrier 1 is suspended from a support 14.
5 vertically. Reference numeral 16 denotes an arm that supports the elevator, and the base 16hfl of the arm 16 is fixed to the moving pedestal 17.

18 is a horizontal movement device that horizontally moves the movable bed. Reference numeral 19 is a surface pressure gauge, and 20 is a grate suspended from the surface pressure gauge, which measures the pressure of the molecular membrane deployed on the liquid filled in each deployment tank of both deployment tanks for one person and IB. It is now possible to do so.

In the above structure, the functions of the gates 11 and 12 at the tips of the fixed barriers 9 and 10 will be explained in detail with reference to FIGS. 4 and 5. The curved portions 11tr and 12th of the gates are in opposing contact with each other with moderate contact pressure.
Figures 4(a) to 4(c) show the state in which the carrier passes between
) and the side view of FIG. 5(a) to FIG. This shows the process of transferring the carrier 15 while it is immersed in the other developing water tank IB so that the developing material 19 does not match. By horizontal movement of the moving ped 17 on the WI B via the arm 16, the carrier 15 passes between the curved parts 11h and 12g of the gates that are in opposing contact with each other, and the curved parts 11h and 12 * indicates the surface 4a of liquid 4, which moves in the direction of arrow P while pressing the carrier surface with appropriate elasticity.
The spread material is scraped off by the barrier curved parts 11a and 12a, and another spread material is spread while remaining immersed in the liquid of the carrier development tank without being mixed into the development tank on the opposite side. can be transferred to the other expansion tank.

Next, the process of forming a monomolecular heterogeneous film will be described with reference to FIGS. 7 and 8.

FIG. 7(a) shows the step in which the carrier 15 is lowered and immersed in the developing water tank where the developing material is deployed above the liquid level 4, and FIG. 7(b) shows the stage where the carrier 15 is being immersed. After being immersed in the liquid, a monomolecular film of substance A is transferred to the surface of the carrier as shown in FIG. 8(a). Next, in FIG. 7(c), the carrier 15 is moved in parallel to the gates 11 and 12.
The next state is shown in FIG. 7(d) when the carrier 15 is raised from the developing tank IB in which the developing substance B is being developed. b)
As shown in FIG.
A monolayer of material is accumulated.

Fig. g7 (b) shows a state in which the carrier 15, which has been raised above the water surface of the development tank IB, is passed over the gate 11.12 and transferred again to the development tank IA. In the same manner, FIG. 7(f) is again expanded with one carrier per person
Figure 8 (c) shows the state in which λ substance is transferred onto the monomolecular film that has already accumulated @AB''' and a cumulative film of 1 BA'' is formed when 5 is lowered. By repeating the same process, ABAB...
・It is possible to form a monomolecular heterolaminar film with an asymmetric structure.

In the above embodiment of the present invention, a single molecule cumulative film deposition apparatus for two types of substances, human and B, was shown, but by further partitioning the top surface of the developing water tank, single molecules with the structure "ABCD..." It is also possible to form a heterogeneous film.

[Effects of the Invention] As described above, the monomolecular cumulative film deposition apparatus of the present invention is a simple device in which the liquid surface is divided into multiple parts by a fixed barrier having a gate that can be opened and closed in the same development water tank. , it is possible to omit the removal work of the next developing material, which was conventionally done in a single developing tank, and it is possible to IF multiple layers of monomolecular heterolaminar films in a short time, thereby reducing the work environment such as oxidation. This has the effect of making it possible to obtain high-quality samples with less soy sauce.

[Brief explanation of drawings]

The drawings show embodiments of the present invention, and the rear and Y-
Figure 3 is a cross-sectional view taken along line 1-1 in Figure 2; Plan view of passing state, Fig. 5 (a
), FIG. 5(b), and FIG. 5(c) are all side views of the state in which the carrier passes between the barriers, FIG. 6 is a plan view showing another embodiment of the barrier, and FIG. 7( Figures 8 (a) to 7 (pupils) are explanatory diagrams showing the state of the carrier and the developing water tank, and Figures 8 (a) to 8 (c) are schematic diagrams showing the state in which the monomolecular film is transferred to the carrier surface. FIG. 1... Deployment water tank, 5 people, 5B... Moving barrier, 9゜10... Fixed barrier, 11.12... Gate, 13
...Elevator, 18...Horizontal movement device. Applicant's representative Patent attorney Takehiko Suzue Figure 2 Figure 3 1'. (a) (b) (c) Figure 4 (a) (b) (c) Figure 5 Figure 6 Procedure amendment form) 1. Display of the case Japanese Patent Application No. 61-089416 2, Name of the invention is monomolecular heterogeneous deoxidation ati 3, Person making the amendment Relationship to the case Patent applicant Kyowa Interface Science Co., Ltd. 4 Agent Kasumigaseki, Chiyoda-ku, Tokyo 3-7-2 UBE Building 〒1
00 Telephone 03 (502) 3181 (main representative)
7. Contents of amendment (1) “Figure 7 (
a) to Figure 7 (Q)" should be corrected to "Figure 7 4."

Claims (1)

[Claims] A fixed barrier with an openable and closable gate that partitions the liquid surface part of a developing water tank that has the same liquid phase to prevent mixing of the developing substances on the liquid surface and allows only the carrier to pass through; It consists of a moving barrier that applies piston pressure to the developing substance on the liquid level provided in each of the multiple expansion tanks, an elevator that lowers and raises the carrier vertically into the liquid, and a horizontal movement device that moves the carrier horizontally. A monomolecular heterogeneous film deposition apparatus characterized by the following.