JPS62253374A - Production of chlorella - Google Patents
Production of chlorellaInfo
- Publication number
- JPS62253374A JPS62253374A JP9604086A JP9604086A JPS62253374A JP S62253374 A JPS62253374 A JP S62253374A JP 9604086 A JP9604086 A JP 9604086A JP 9604086 A JP9604086 A JP 9604086A JP S62253374 A JPS62253374 A JP S62253374A
- Authority
- JP
- Japan
- Prior art keywords
- chlorella
- grinding
- crushing
- cell wall
- stone
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 241000195649 Chlorella <Chlorellales> Species 0.000 title claims abstract description 44
- 238000004519 manufacturing process Methods 0.000 title claims description 6
- 239000004575 stone Substances 0.000 claims abstract description 20
- 210000002421 cell wall Anatomy 0.000 claims abstract description 17
- 235000019621 digestibility Nutrition 0.000 claims abstract description 12
- 210000004027 cell Anatomy 0.000 claims abstract description 8
- 230000015556 catabolic process Effects 0.000 abstract 1
- 238000006731 degradation reaction Methods 0.000 abstract 1
- 238000000034 method Methods 0.000 description 9
- 102000057297 Pepsin A Human genes 0.000 description 6
- 108090000284 Pepsin A Proteins 0.000 description 6
- 229940111202 pepsin Drugs 0.000 description 6
- 239000000047 product Substances 0.000 description 5
- 230000003834 intracellular effect Effects 0.000 description 3
- 102000004169 proteins and genes Human genes 0.000 description 3
- 108090000623 proteins and genes Proteins 0.000 description 3
- 240000009108 Chlorella vulgaris Species 0.000 description 2
- 235000007089 Chlorella vulgaris Nutrition 0.000 description 2
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 description 2
- -1 EPA) Chemical class 0.000 description 2
- 102000004190 Enzymes Human genes 0.000 description 2
- 108090000790 Enzymes Proteins 0.000 description 2
- 241001465754 Metazoa Species 0.000 description 2
- 239000002253 acid Substances 0.000 description 2
- 150000007513 acids Chemical class 0.000 description 2
- 239000004480 active ingredient Substances 0.000 description 2
- 230000006866 deterioration Effects 0.000 description 2
- 229940088598 enzyme Drugs 0.000 description 2
- 239000000843 powder Substances 0.000 description 2
- 238000002604 ultrasonography Methods 0.000 description 2
- 241000195652 Auxenochlorella pyrenoidosa Species 0.000 description 1
- 235000007091 Chlorella pyrenoidosa Nutrition 0.000 description 1
- 241000195628 Chlorophyta Species 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 150000001720 carbohydrates Chemical class 0.000 description 1
- 235000014633 carbohydrates Nutrition 0.000 description 1
- 229930002875 chlorophyll Natural products 0.000 description 1
- 235000019804 chlorophyll Nutrition 0.000 description 1
- ATNHDLDRLWWWCB-AENOIHSZSA-M chlorophyll a Chemical compound C1([C@@H](C(=O)OC)C(=O)C2=C3C)=C2N2C3=CC(C(CC)=C3C)=[N+]4C3=CC3=C(C=C)C(C)=C5N3[Mg-2]42[N+]2=C1[C@@H](CCC(=O)OC\C=C(/C)CCC[C@H](C)CCC[C@H](C)CCCC(C)C)[C@H](C)C2=C5 ATNHDLDRLWWWCB-AENOIHSZSA-M 0.000 description 1
- 235000014113 dietary fatty acids Nutrition 0.000 description 1
- 230000029087 digestion Effects 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 229930195729 fatty acid Natural products 0.000 description 1
- 239000000194 fatty acid Substances 0.000 description 1
- 150000004665 fatty acids Chemical class 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 235000012041 food component Nutrition 0.000 description 1
- 230000020169 heat generation Effects 0.000 description 1
- 229910052500 inorganic mineral Inorganic materials 0.000 description 1
- 210000000936 intestine Anatomy 0.000 description 1
- 150000002632 lipids Chemical class 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 239000011707 mineral Substances 0.000 description 1
- 235000010755 mineral Nutrition 0.000 description 1
- 238000006386 neutralization reaction Methods 0.000 description 1
- 235000008935 nutritious Nutrition 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 230000000144 pharmacologic effect Effects 0.000 description 1
- 238000000053 physical method Methods 0.000 description 1
- 230000035939 shock Effects 0.000 description 1
- 238000007086 side reaction Methods 0.000 description 1
- 210000002784 stomach Anatomy 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Landscapes
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
Description
【発明の詳細な説明】
＋８）産業上の利用分野
本発明は細胞壁が破砕されたクロレラの製造方法に関す
るものである。Detailed Description of the Invention +8) Industrial Application Field The present invention relates to a method for producing chlorella whose cell walls have been crushed.
（ｂｌ従来の技術
クロレラは直径２〜１０μｍの単細胞緑藻の一種でアミ
ノ酸バランスの優れた蛋白質資源（乾物中蛋白質５０％
以上）であり、また豊富なビタミン、ミネラル、葉緑素
資源であるため栄養自然食品として広く利用されている
。さらにクロレラ中の糖質、脂質（例えばＥＰＡ）その
他各種成分の薬理的効果も研究され、各種疾病への有効
性が期待されている。(bl Conventional technology Chlorella is a type of unicellular green algae with a diameter of 2 to 10 μm. It is a protein resource with an excellent amino acid balance (50% protein in dry matter)
(above), and it is widely used as a nutritious natural food because it is rich in vitamins, minerals, and chlorophyll resources. Furthermore, the pharmacological effects of carbohydrates, lipids (e.g., EPA), and other various components in chlorella have been studied, and their effectiveness against various diseases is expected.
一方クロレラは硬い細胞壁を有し、このため動物や人間
の胃ないし腸内では消化され難く各種栄養成分を有効に
利用することが困難であった。そこで近年、酸やアルカ
リあるいは酸素などを用いて化学的にあるいは機械的衝
撃（ボールミルなど）や超音波などを用いて物理的に細
胞壁を破壊し消化率をあげ細胞内成分を有効に利用する
方法が見出されている。On the other hand, chlorella has a hard cell wall, which makes it difficult to digest in the stomachs or intestines of animals and humans, making it difficult to effectively utilize various nutritional components. Therefore, in recent years, methods have been developed to increase the digestibility and effectively utilize intracellular components by destroying cell walls chemically using acids, alkalis, or oxygen, or physically using mechanical shock (such as a ball mill) or ultrasound. has been found.
（ｅ）発明が解決しようとする問題点
しかしながら化学的な細胞壁破砕方法では消化率の高い
クロレラが得られるが酸やアルカリ、酵素などを使用し
、さらに水が加わるため副反応やフエオホルバイドの生
成による品質の劣化が考えられる。(e) Problems to be solved by the invention However, although the chemical cell wall crushing method yields highly digestible chlorella, it uses acids, alkalis, enzymes, etc., and water is added, resulting in side reactions and the formation of pheophorbide. Possible quality deterioration.
また中和や酵素除去などの破砕後の処理が加わるため、
作業性の面で経済的でなく、また排水面での問題もある
。In addition, post-shredding processes such as neutralization and enzyme removal are added, so
It is not economical in terms of workability and also has problems in terms of drainage.
一方機械的衝撃や超音波による物理的破砕方法は簡便で
はあるが、均質な細胞壁破砕品を得ることがむずかしく
各クロレラ細胞の破砕度にばらつきがあるため、消化率
の向上に限界がある。On the other hand, physical crushing methods using mechanical impact or ultrasound are simple, but there are limits to improving digestibility because it is difficult to obtain a homogeneous cell wall crushed product and the degree of crushing of each Chlorella cell varies.
本発明の目的は、人間あるいは動物がクロレラの細胞内
有効成分を最も効率的に摂取できるような、細胞壁破砕
クロレラを簡便にしかも低コス゛トで得ることにある。An object of the present invention is to easily obtain cell-wall-disrupted chlorella at a low cost so that humans or animals can most efficiently ingest the intracellular active ingredients of chlorella.
ｆｄ１問題点を解決するための手段
本発明者らは上記の目的を達成すべく鋭意研究の結果、
クロレラを磨砕粉砕すると、消化率を高めることができ
、とくに石臼あるいは石臼と同等の機能を有する機器を
用いて磨砕粉砕すると効果的であることを見出し、本発
明を完成した。Means for Solving the FD1 Problems The inventors of the present invention have conducted extensive research to achieve the above objectives, and have found that:
The present invention was completed based on the discovery that the digestibility of chlorella can be increased by grinding and grinding, and that it is particularly effective to grind and grind using a stone mill or a device having the same function as a stone mill.
即ち本発明によれば、クロレラを磨砕粉砕することによ
り細胞壁を破砕し、消化率を高めることを特徴とする細
胞壁破砕クロレラの製造方法が提供される。That is, according to the present invention, there is provided a method for producing cell wall-crushed chlorella, which is characterized by crushing cell walls and increasing digestibility by grinding and crushing chlorella.
前記の石臼と同等の機能を有する機器とは、スーパーグ
ラインディングミル６００型（株式会社あいゃ製）など
のような試料を２つの盤の間ですりつぶす機能を持った
機器をさす。同じ物理的方法である従来の衝撃による破
砕方法に比べ磨砕による破砕方法は物質を超微粉末化す
るのに通し、より細かく均質にクロレラの細胞壁を破砕
することができる。The device having the same function as the stone mill mentioned above refers to a device having the function of grinding a sample between two plates, such as Super Grinding Mill Model 600 (manufactured by Aya Co., Ltd.). Compared to the conventional impact-based crushing method, which is the same physical method, the grinding-based crushing method transforms the material into ultra-fine powder, and can crush the cell walls of chlorella more finely and homogeneously.
またこの時クロレラの処理能力は石臼の磨砕面（石盤）
の表面積、ホッパーからの投入量、石臼の回転速度など
と関係するが、単位時間、単位石盤表面積あたりのクロ
レラ処理量が３〜１５ｇ／−・ｈの範囲になるよう調整
することが好ましい。Also, at this time, the processing capacity of chlorella is the grinding surface of the stone mill (stone disk).
It is preferable to adjust the amount of chlorella processed per unit time and unit stone surface area to be in the range of 3 to 15 g/-.h, although this is related to the surface area of the hopper, the amount of input from the hopper, the rotation speed of the stone mill, etc.
単位時間あたりの投入量を多くすると細胞壁破砕率が低
くなり、また回転速度をあげると発熱によるクロレラの
品質劣化が見られるため、上記範囲以上は好ましくなく
、また上記範囲以下では作業効率が悪く経済的ではない
。If the amount of input per unit time is increased, the cell wall crushing rate will be lowered, and if the rotation speed is increased, the quality of chlorella will deteriorate due to heat generation. Not the point.
本発明の方法に用いられるクロレラは、クロレラブルガ
リス（Ｃｈｌｏｒｅｌｌａ ｖｕｌｇａｒｉｓ）クロレ
ラエリプソイブイア（Ｃｈｌｏｒｅｌｌａ ｅｌｌｉｐ
ｓｏｉｄｉａ）クロレラピレノイドサ（Ｃｈｌｏｒｅｌ
ｌａ ｐｙｒｅｎｏｉｄｏｓａ）クロレラミニュテイシ
マ（Ｃｈｌｏｒｅｌｌａ ＋＊ｉｎｕｔｉｓｓｉｍａ）
などである。The chlorella used in the method of the present invention includes Chlorella vulgaris, Chlorella ellipsobuia, and Chlorella ellipsobuia.
soidia) Chlorella pyrenoidosa (Chlorel
la pyrenoidosa) Chlorella ++ inutissima
etc.
（ｅ）実施例
実施例１
クロレラ（Ｃｈｌｏｒｅｌｌａ ｍｉｎｕｔｉｓｓｉｍ
ａ乾燥品）をスーパーグラインディングミル６００型（
石盤の直径６０ＯＮ株式会社あいゃ製）を用い１８０ｇ
／ｈ （６，４ｇ／４００−・ｈ）の処理速度で磨砕粉
砕した。得られた細胞壁破砕クロレラのペプシン消化率
は未処理品の３９．６％に対し８０゜０％まで向上した
。(e) Examples Example 1 Chlorella (Chlorella minutissim)
a dry product) in a super grinding mill model 600 (
180g using a stone plate with a diameter of 60ON (manufactured by Aya Co., Ltd.)
Grinding was carried out at a processing speed of /h (6.4 g/400-.h). The pepsin digestibility of the resulting cell wall-crushed Chlorella was improved to 80.0%, compared to 39.6% for the untreated product.
なおペプシン消化率の測定方法は次の方法に依った。The method for measuring pepsin digestibility was as follows.
試料１ｇにエーテルを加えて懸濁させ上澄を除き、ペプ
シン−ＨＣｌ溶液（ＨＣ１０，０７５Ｎ。Ether was added to 1 g of the sample to suspend it, the supernatant was removed, and a pepsin-HCl solution (HC10,075N) was added.
ペプシン０．２％）を１５０ｍ１加え、４５℃で１６時
間インキュベートしたのち吸引濾過し、残渣を分解チュ
ーブに入れたん蛋白量を測定し、下式からペプシン消化
率を算出する。Add 150ml of pepsin (0.2%), incubate at 45°C for 16 hours, filter by suction, put the residue into a digestion tube, measure the amount of protein, and calculate the pepsin digestibility using the formula below.
ペプシン消化率（％）＝
ブランクの蛋白量（％）−未消化蛋白量（％）×１００
実施例２
クロレラ（Ｃｈｌｏｒｅｌｌａ ｖｕ１ｇａｒｉｓ乾燥
品）を石臼（石盤の直径３３０ｍｍ）を用い８５ｇ／ｈ
（１０ｇ／１００−・ｈ）の処理速度で磨砕粉砕した。Pepsin digestibility (%) = Blank protein content (%) - Undigested protein content (%) x 100 Example 2 Chlorella (Chlorella vulgaris dried product) was harvested at 85 g/h using a stone mill (stone diameter 330 mm).
Grinding was carried out at a processing speed of (10 g/100-h).
得られた細胞壁破砕クロレラのペプシン消化率は未処理
品の５０．０％に対し９３．５％まで向上した。The pepsin digestibility of the resulting cell wall-crushed Chlorella was improved to 93.5%, compared to 50.0% for the untreated product.
（ｆ１発明の効果
本発明のクロレラの製造方法は、クロレラの細胞壁を磨
砕粉砕によって破砕するものであり、とくに石臼または
石臼と同等の機能を有する機器を用いるとクロレラの細
胞同士および石臼とクロレラが摩擦しあって粉砕される
ため、強じんなりロレラの細胞壁も均質に破砕すること
ができ衝撃による破砕方法よりさらに消化率の高いクロ
レラを得ることかで゛きる。(f1 Effects of the Invention The method for producing chlorella of the present invention is to crush the cell walls of chlorella by grinding and crushing, and in particular, when a stone mill or a device having the same function as a stone mill is used, the cells of chlorella are separated from each other, and between the stone mill and chlorella. Since they are crushed by friction, even the tough cell walls of Lorella can be homogeneously crushed, making it possible to obtain Chlorella with higher digestibility than by crushing by impact.
また本発明の方法はクロレラ粉末をそのまま、連続的に
処理することができるため、低コストで経済的である。In addition, the method of the present invention is economical at low cost because chlorella powder can be continuously processed as it is.
さらに破砕されたクロレラは順次送られ回収されるので
長時間石臼の間に残されるということはなく品質の劣化
がない。Furthermore, since the crushed chlorella is sent and collected one by one, it is not left between the stone mills for a long time, so there is no deterioration in quality.
さらにまた、細胞壁を取り去るのではないため、クロレ
ラの細胞内有効成分は細胞壁につつまれミ細胞壁破砕後
も品質が安定である。例えば、クロレラとしてＥＰＡを
含むもの（Ｃｈｌｏｒｅｌｌａ ｎ＋ｉｎｕｔｉｓｓｉ
ｍａなど）を用いた場合、ＥＰＡは酸化されやすい脂肪
酸であるが、本発明で得られる細胞壁破砕クロレラはＥ
ＰＡの品質が安定である。Furthermore, since the cell wall is not removed, the intracellular active ingredients of Chlorella are surrounded by the cell wall and remain stable in quality even after the cell wall is disrupted. For example, those containing EPA as chlorella (Chlorella n+inutissi
EPA is a fatty acid that is easily oxidized, but the cell wall-disrupted chlorella obtained in the present invention is
The quality of PA is stable.
Claims (2)
し、消化率を高めることを特徴とする細胞壁破砕クロレ
ラの製造方法。(1) A method for producing cell wall-crushed chlorella, which is characterized by crushing cell walls and increasing digestibility by grinding and crushing chlorella.
機器を用いて行い、かつ単位時間、単位石盤表面積あた
りのクロレラの処理量が、３〜１５ｇ／１００ｃｍ＾３
・ｈの範囲にある特許請求の範囲第（１）項記載の製造
方法。(2) Grinding and crushing is performed using a stone mill or equipment with the same function as a stone mill, and the amount of chlorella processed per unit time and unit stone surface area is 3 to 15 g/100 cm^3
- The manufacturing method according to claim (1) falling within the scope of h.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP9604086A JPS62253374A (en) | 1986-04-24 | 1986-04-24 | Production of chlorella |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP9604086A JPS62253374A (en) | 1986-04-24 | 1986-04-24 | Production of chlorella |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JPS62253374A true JPS62253374A (en) | 1987-11-05 |
Family
ID=14154378
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP9604086A Pending JPS62253374A (en) | 1986-04-24 | 1986-04-24 | Production of chlorella |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS62253374A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH11171782A (en) * | 1997-12-03 | 1999-06-29 | Kaoru Yamane | Production of liquid shark cartilage |
-
1986
- 1986-04-24 JP JP9604086A patent/JPS62253374A/en active Pending
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH11171782A (en) * | 1997-12-03 | 1999-06-29 | Kaoru Yamane | Production of liquid shark cartilage |
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