JPS62253321A - Inoculation of shiitake fungi - Google Patents

Abstract

(57) [Summary] This bulletin contains application data before electronic filing, so abstract data is not recorded.

Description

DETAILED DESCRIPTION OF THE INVENTION <Industrial Application Field> The present invention relates to a method of inoculating mushrooms that improves the efficiency of inoculating work and allows shiitake fungi to spread throughout the log in a short period of time.

<Conventional technology and its v4 issues> Shiitake cultivation has traditionally involved cutting logs such as sawtooth oak and oak into pieces, cutting them into a certain length, making many small holes, and inserting secondary mushrooms into the holes. A method of planting seeds using sawdust seed fungi or wood chips that have cultivated mycelium has been adopted. The process is based on the idea that the logs used to varying degrees must be sufficiently dry and withered.

This conventional method is very time-consuming as it requires drilling many small holes in the log and then embedding the seeds, and it also introduces unnecessary harmful bacteria into the log when opening the holes. It may invade, and it may take up to 15 days for secondary mycelium to spread from the planted seed to the entire log and turn the entire log into a hodder.
Since it takes a long time of ~18 months, there is a problem that the overall production efficiency is low.

<Means for Solving the Problems> In solving the above problems, the inventors of the present invention discovered that since the shiitake fungus is a saprophytic fungus, it grows into what is called a mushroom (fruiting body).
Although it is true that the log must be withered and dead in order for this to occur, the experiment was conducted based on the basic idea that there would be no problem even if the log was still alive when inoculating the bacteria for the first time. As a result, the present invention was developed, and the gist of the present invention is to immerse the cut end of a freshly felled log in a culture solution containing secondary mycelia of shiitake mushrooms, and to absorb the transpiration effect of the leaves of the log. This is a method for inoculating shiitake fungi, which is characterized by transferring secondary hyphae into logs together with a culture solution.

The method of the present invention needs to be carried out while the log is still alive and has the ability to suck up the solution through the pump action associated with the physiological phenomenon of leaf transpiration. It should be started immediately and the log should be used in its original state with its twigs and leaves. The volume of culture solution containing secondary shiitake mushroom hyphae is approximately 5 cm when the diameter of the ice test is about 10 to 15 cIllI.
00ce, the diameter of the log is about 20-25cIn, 100
0ee position, 20-25 cIn position, 1500ee position, 30
For logs larger than cm, it is sufficient to absorb about 2'0OOce, and this amount of culture solution is absorbed in about one day and night, but after that, leave it for 2 to 3 days to ensure that the culture solution is distributed throughout the log.

Also, cut the cut end of the log diagonally to make it as easy to absorb the culture solution as possible, or peel off the skin around the cut edge, and place the cut end in an airtight bag immediately after cutting it so that the cut end does not come in contact with the air as much as possible. The bag is immersed in the stored culture solution, and preferably an air release mechanism is attached to the airtight bag.

After the shiitake secondary mycelia are transferred to every corner of the log along with the culture solution in this way, the shiitake mushrooms are cut into appropriate lengths to form hoda trees, and shiitake mushrooms are produced by the usual method.

<Examples and operations> The method of the present invention will be described in detail below with reference to the drawings showing examples of the present invention.

A sawtooth oak with a diameter of about 20 cm at the cut end was selected as the log (1), and after cutting it down, the twigs and leaves remained intact.
The skin around the cut end was peeled off, and the cut end was placed in an airtight container (3) containing a culture solution (2) containing secondary shiitake mushroom mycelium, as shown in the figure, and kept airtight. The airtight container (3) is provided with an air vent hole (4), and while the air is removed from the air vent hole, the culture solution is injected through the separately provided culture solution inlet (5), and if left in that state, approximately The culture solution continued to be absorbed into the logs for up to a day and a half, but after that it stopped decreasing significantly.

Thereafter, the log (1) was removed from the airtight container (3) and left for another 3 days, cut into pieces about 120 cm in length, and left lying face down with branches and leaves placed over them to prevent direct sunlight. When the log that had been cut into pieces was observed about three months later, it was confirmed that white primordia had been formed over the entire area of the log.

<Effects of the Invention> As described above, the method of the present invention utilizes the life effect of the log while the log is still alive and active, which is completely different from conventional common sense, and allows shiitake mushrooms to be injected into the log. This is a new method for distributing secondary mycelia, and compared to the conventional method of using coma fungi and sawdust fungi, this method reduces the need for drilling holes in logs and improves work efficiency. At the same time, various tools such as an electric drill and their power sources are not required, and drilling the holes can also prevent the intrusion of harmful bacteria during work.

In addition, in the method of the present invention, the shiitake secondary hyphae begin their activity throughout the entire area of the log, so the period until the primordium is formed is shortened to 3 months compared to about 6 months in the conventional method, which increases the production efficiency of shiitake mushrooms. It will improve.

The method of the present invention can also be applied to the production of mushrooms such as jellyfish and nameko mushrooms.

[Brief explanation of drawings]

The drawing is a partially cutaway perspective view for explaining the present invention in detail. In the diagram, (1): Log (2): Culture solution (3): Sealed container (4): Air vent hole (5) ゛Culture solution inlet

Claims (1)

[Claims] 1. The cut end of a freshly felled log is immersed in a culture solution containing secondary mycelia of shiitake mushrooms, and the secondary mycelium is transferred into the log along with the culture solution using the transpiration effect of the leaves of the log. A distinctive method of inoculating shiitake mushrooms.