JPS62247254A - 核酸の塩基配列決定のための信号処理方法 - Google Patents

Info

Publication number

JPS62247254A

JPS62247254A JP61090606A JP9060686A JPS62247254A JP S62247254 A JPS62247254 A JP S62247254A JP 61090606 A JP61090606 A JP 61090606A JP 9060686 A JP9060686 A JP 9060686A JP S62247254 A JPS62247254 A JP S62247254A

Authority

JP

Japan

Prior art keywords

band

base sequence

autoradiograph

nucleic acid

determining

Prior art date

1986-04-19

Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)

Granted

Links

• Espacenet
• Global Dossier
• Discuss

• 150000007523 nucleic acids Chemical class 0.000 title claims abstract description 36
• 102000039446 nucleic acids Human genes 0.000 title claims abstract description 35
• 108020004707 nucleic acids Proteins 0.000 title claims abstract description 35
• 238000012545 processing Methods 0.000 title claims description 12
• 238000000926 separation method Methods 0.000 claims abstract description 25
• 238000011161 development Methods 0.000 claims abstract description 24
• 238000001514 detection method Methods 0.000 claims abstract description 20
• 238000000034 method Methods 0.000 claims description 37
• 239000012634 fragment Substances 0.000 claims description 28
• OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 claims description 27
• 239000000126 substance Substances 0.000 claims description 21
• 239000000203 mixture Substances 0.000 claims description 15
• 238000003672 processing method Methods 0.000 claims description 15
• 239000000463 material Substances 0.000 claims description 10
• OPTASPLRGRRNAP-UHFFFAOYSA-N cytosine Chemical compound NC=1C=CNC(=O)N=1 OPTASPLRGRRNAP-UHFFFAOYSA-N 0.000 claims description 8
• UYTPUPDQBNUYGX-UHFFFAOYSA-N guanine Chemical compound O=C1NC(N)=NC2=C1N=CN2 UYTPUPDQBNUYGX-UHFFFAOYSA-N 0.000 claims description 8
• 230000008569 process Effects 0.000 claims description 6
• RWQNBRDOKXIBIV-UHFFFAOYSA-N thymine Chemical compound CC1=CNC(=O)NC1=O RWQNBRDOKXIBIV-UHFFFAOYSA-N 0.000 claims description 6
• 229940104302 cytosine Drugs 0.000 claims description 4
• GFFGJBXGBJISGV-UHFFFAOYSA-N Adenine Chemical compound NC1=NC=NC2=C1N=CN2 GFFGJBXGBJISGV-UHFFFAOYSA-N 0.000 claims description 3
• 229930024421 Adenine Natural products 0.000 claims description 3
• 229960000643 adenine Drugs 0.000 claims description 3
• 229940113082 thymine Drugs 0.000 claims description 3
• 230000005284 excitation Effects 0.000 claims description 2
• 230000001678 irradiating effect Effects 0.000 claims description 2
• 238000012163 sequencing technique Methods 0.000 claims description 2
• 230000007480 spreading Effects 0.000 claims description 2
• 230000004927 fusion Effects 0.000 abstract description 7
• 230000005855 radiation Effects 0.000 description 15
• 230000002285 radioactive effect Effects 0.000 description 11
• 238000001962 electrophoresis Methods 0.000 description 8
• 150000001875 compounds Chemical class 0.000 description 7
• 230000005012 migration Effects 0.000 description 7
• 238000013508 migration Methods 0.000 description 7
• 238000010586 diagram Methods 0.000 description 5
• 238000006243 chemical reaction Methods 0.000 description 4
• 238000003776 cleavage reaction Methods 0.000 description 4
• 239000000047 product Substances 0.000 description 4
• 230000007017 scission Effects 0.000 description 4
• 238000000376 autoradiography Methods 0.000 description 3
• 230000002068 genetic effect Effects 0.000 description 3
• 230000007018 DNA scission Effects 0.000 description 2
• ISAKRJDGNUQOIC-UHFFFAOYSA-N Uracil Chemical compound O=C1C=CNC(=O)N1 ISAKRJDGNUQOIC-UHFFFAOYSA-N 0.000 description 2
• 230000008859 change Effects 0.000 description 2
• 238000003384 imaging method Methods 0.000 description 2
• 238000004809 thin layer chromatography Methods 0.000 description 2
• 101100136092 Drosophila melanogaster peng gene Proteins 0.000 description 1
• 229910052693 Europium Inorganic materials 0.000 description 1
• 240000007594 Oryza sativa Species 0.000 description 1
• 235000007164 Oryza sativa Nutrition 0.000 description 1
• 238000004458 analytical method Methods 0.000 description 1
• 230000008901 benefit Effects 0.000 description 1
• 230000015572 biosynthetic process Effects 0.000 description 1
• 230000000052 comparative effect Effects 0.000 description 1
• 230000000295 complement effect Effects 0.000 description 1
• 239000002131 composite material Substances 0.000 description 1
• 238000000354 decomposition reaction Methods 0.000 description 1
• 239000007857 degradation product Substances 0.000 description 1
• -1 europium activated barium fluoride bromide Chemical class 0.000 description 1
• 239000012535 impurity Substances 0.000 description 1
• 230000010365 information processing Effects 0.000 description 1
• 238000005259 measurement Methods 0.000 description 1
• 230000007246 mechanism Effects 0.000 description 1
• 238000004816 paper chromatography Methods 0.000 description 1
• 239000002985 plastic film Substances 0.000 description 1
• 229920006255 plastic film Polymers 0.000 description 1
• 229920000642 polymer Polymers 0.000 description 1
• 238000002360 preparation method Methods 0.000 description 1
• 230000001681 protective effect Effects 0.000 description 1
• 238000002601 radiography Methods 0.000 description 1
• 238000001959 radiotherapy Methods 0.000 description 1
• 230000010076 replication Effects 0.000 description 1
• 235000009566 rice Nutrition 0.000 description 1
• 150000003839 salts Chemical class 0.000 description 1
• 238000003786 synthesis reaction Methods 0.000 description 1
• 238000012360 testing method Methods 0.000 description 1
• 235000011178 triphosphate Nutrition 0.000 description 1
• 239000001226 triphosphate Substances 0.000 description 1
• UNXRWKVEANCORM-UHFFFAOYSA-N triphosphoric acid Chemical compound OP(O)(=O)OP(O)(=O)OP(O)(O)=O UNXRWKVEANCORM-UHFFFAOYSA-N 0.000 description 1
• 229940035893 uracil Drugs 0.000 description 1
• 230000000007 visual effect Effects 0.000 description 1