JPS62220598A - Sanitary product containing encapsulated enzyme - Google Patents

Abstract

(57) [Summary] This bulletin contains application data before electronic filing, so abstract data is not recorded.

Description

DETAILED DESCRIPTION OF THE INVENTION (a) Industrial Application Field The present invention relates to sanitary products such as shampoos, conditioners, body soaps, and liquid bath additives containing encapsulated enzymes.

(b) Conventional Technology Various sanitary products containing enzymes mixed in a base have been developed, focusing on the deproteinizing, anti-inflammatory and anti-inflammatory effects of enzymes.

However, due to the nature of enzymes, it is difficult to stabilize the enzyme activity over the long term.Especially, enzymes are in solution form, contain water, and contain many surfactants, making long-term stabilization extremely difficult. be.

Therefore, methods have been developed to achieve stabilization through chemical stabilization of the components or physical methods.

For example, enzyme immobilization techniques are known in which enzymes are wrapped in polymers such as sericin, fibron, or lauromacrogol to make them water-insoluble.

(c) Problems to be Solved by the Invention However, although such conventional techniques can stabilize the enzyme activity to a certain extent, there is a problem in terms of the enzyme activity expression rate during use. That is, since the enzyme itself is immobilized with sericin, fibroin, etc., it has poor reactivity with substrates and has the problem of not being able to expect an effective enzyme activity effect in a short period of time.

Furthermore, in the past, it has been impossible to separate and quantify each enzyme in a product containing a plurality of fermentation straws, particularly in a product containing a plurality of enzymes having similar substrate activities.

SUMMARY OF THE INVENTION An object of the present invention is to provide a sanitary product containing an encapsulated enzyme that can solve the problems of the prior art described above.

(d) Means and structure for solving the problems The present invention includes:
This relates to a sanitary product manufactured by mixing an encapsulated enzyme in a sanitary product base.

Here, sanitary products refer to general liquid, cream, solid, or powder sanitary products, including shampoo, conditioner, body soap, liquid bath additives, liquid detergent, toothpaste, lotion (basic cosmetics), etc. Refers to a product.

Further, the enzyme contained in the capsule can be appropriately selected depending on the use of the sanitary product, and includes, for example, protease, lipolytic enzyme, and anti-inflammatory enzyme. In addition, as proteolytic enzymes, ASP protease, papain,
Promelain and the like are used, the lipolytic enzyme is ASP lipase and the like, and the anti-inflammatory enzyme is lysozyme chloride and the like.

Next, the structure of the capsule containing the above enzyme will be explained.

■First, referring to the particle diameter and capsule content of the capsule, the particle diameter is preferably 0.3 to 5.5 mm, taking into account the encapsulation technology and the commercial value when commercialized. The capsule content (capsule + core material) based on the total weight of the product is 0.03 to 9% by weight, considering that if it is too small, the capsules will not be uniformly dispersed in the base, and if it is too large, the product value will be impaired. It is preferable that

However, the type of capsule is not particularly limited as long as it is easily breakable, that is, easily destroyed by physical stress during use, or easily water-soluble.

■The wall material of the capsule varies depending on the purpose of use and product characteristics of the sanitary product, but it may be destroyed by physical stress during use, or it may be dissolved in water, etc. It is sufficient if it shows the effective activity of the h enzyme released to the outside.

Preferred capsule wall materials used in the present invention are broadly classified into water-soluble wall materials and water-insoluble wall materials.

Examples of water-soluble wall materials include those using water-soluble polymers. Examples of such water-soluble polymers include polysaccharides and cellulose derivatives such as carrageenan, methylcellulose, pectin, alginic acid, amylopectin, guar gum, ethylcellulose, hydroxyethylcellulose, hydroxypropylcellulose, and carboxymethylcellulose, as well as gelatin and polyvinyl alcohol. etc.

However, when the above-mentioned water-soluble polymer is used as a capsule wall material, it is necessary to make the capsule wall material insoluble or to prevent it from dissolving in the composition, depending on the sanitary product in which the capsule is blended.

Methods for insolubilizing such capsules include methods that use cross-linking agents, as well as methods that reduce or prevent solubility by adjusting the pH of the components, blending electrolyte components, blending poor solvents for polymers, etc. Become.

Valley 4. -T. Fu, J. Shiishi U. Chuta 1. One bite of water-soluble encapsulated enzyme is required. Conventionally, as representative water-soluble capsule wall material components,
Gelatin is used, but the problem in this case is that when it is poured into a bathtub, an oil film floats on the water surface, and gelatin becomes insolubilized over time.

Therefore, to prevent the formation of an oil film, by selecting oils, fats, surfactants, etc. and adding them in appropriate amounts, oily substances can be dispersed in hot water.
It becomes possible to melt it.

On the other hand, gelatin insolubilization is often caused by aldehyde groups or ketone groups in molecules such as perfumes and surfactants bonding with amino groups in gelatin molecules, but rapid solubility is This is a critical problem for the required bath capsules. Therefore, for insolubility of capsules, for example when using gelatin,
This problem can be solved by using Dogelatin (M-Ko), a modification in which the amino group in the gelatin molecule is replaced with succinic acid or phthalic acid.

The soft encapsulation technology used in the application to the island 1b is 1.15 years old.
- This can be done by any of the following encapsulation techniques.

If the shell of a soft capsule is made of a single wall material such as gelatin, it will break due to external impact, so it is necessary to give the gelatin shell elasticity and flexibility. Therefore, a capsule wall material sheet is prepared by adding glycerin, sorbitol, or the like as a plasticizer to the base material serving as the capsule wall material.

Soft capsules are manufactured using a conventional rotary method or a drip method. In this case, products manufactured using the rotary method generally have a seam in the capsule, and products manufactured using the drip method generally do not have a seam in the capsule. In addition, the manufacturing method using the dropping method generally requires 2
It is used to make small capsules of about 0 to 0.5 cm.

■Enzyme can be packed by filling the enzyme directly as a powder, or by suspending and dispersing the enzyme powder in a liquid or oily substance such as oil, propylene glycol, butylene glycol, or polyhydric alcohol such as glycerin. Can be filled.

In this case, depending on the dispersion, the final undiluted solution may have a high or low viscosity, so the selection of the type of dispersion, as well as the formulation of enzyme stabilizers, excipients, etc. It is necessary to adjust the type and blending ratio.

For example, the appropriate blending ratio for the enzyme powder component is 30x, which is the maximum amount of the packed component. However, the enzyme powder itself
Conditions differ between those with very high bulk density and those with very low bulk density.

(2) The capsules used in the present invention may be colored with red, blue, yellow, etc., or a mixture of these colors, or may be given pearlescent luster, in order to enhance their aesthetic appearance.

(2) Also, by changing the thickness of the capsule wall, it is possible to adjust the degree of breakage due to physical stress during use, or to adjust fJI parameters such as solubility in water and dissolution time.

■Even when multiple enzyme capsules are combined, enzyme activity can be separated and quantified by changing the particle size of each enzyme capsule.

■With regard to the problem between the solution inside the capsule and the wall material, when incorporating encapsulated enzymes into sanitary products, depending on the intended product, the type of enzyme or the wall material must be considered. Depending on the type of capsule wall material, the enzyme may react as a substrate, resulting in the capsule wall material being dissolved. Therefore, when considering a capsule wall material, the first thing to consider is that it does not have substrate properties for enzymes, and that the enzyme dispersion liquid does not pose a problem with the long-term stability of enzymes.

In a composition containing such an enzyme capsule, the difference in specific gravity between the composition and the capsule is adjusted within a range of ±0.1 so that the capsule itself is dispersed as uniformly as possible in the sanitary product composition. It is preferable to do so.

[Phase] When filling the capsule with an enzyme in the form of a dispersion, the dispersion used for dispersing the enzyme is preferably hydrophilic due to the nature of the product being a sanitary product.

(e) Function and effect - The present invention is a method of incorporating enzymes into sanitary products as capsules. Therefore, during use, the capsule itself may be destroyed by physical stress or dissolved in water, etc. Since it is released by the enzyme that is the core substance of the capsule, it is possible to prevent the enzyme protein from being denatured by the composition for a long period of time, and not only can the enzyme activity be stabilized for a long period of time, but it is also effective in a short period of time when used. Enzymatic effects can be exerted.

- Encapsulated enzymes have a different shape from other ingredients, so they can give consumers a sense of security that they contain enzymes.

■By encapsulating enzymes, it is possible to
In particular, for products that contain multiple enzymes with similar substrate activity, it has been impossible to separate and quantify the enzymes, but now this is possible, allowing enzyme analysis necessary to ensure the quality of the product. Quantitative understanding becomes possible.

(f) Examples Next, the present invention will be explained in more detail based on examples.

[Example 1] Example 1 is a case where the present invention was applied to shampoo.

Shampoo is a hair and scalp cleansing agent, and its purpose is to cleanse the scalp and hair by removing various dirt, sebum, dandruff, and leftover hair styling products. Therefore,
As the enzyme component, it is preferable to include a proteolytic enzyme, a lipolytic enzyme, etc.

Therefore, in this example, encapsulated proteolytic enzymes A and B and encapsulated lipolytic enzyme C are blended into the shampoo composition.

In addition, in order to compare the effect of the encapsulated enzyme in this example with that of a conventional shampoo, Comparative Example 1 (composition is the same as Example 1, but the enzyme is not encapsulated. However, the potency in the product ) are prepared, and the component compositions of both are shown on the first back.

Table 1 It is le.

Changes in the enzyme activity rate over time in Example 1 and Comparative Example 1 are shown in the graphs of FIGS. 1 to 3.

The storage conditions for the product for measuring changes over time to investigate enzyme activity stability were room temperature and 40"C.
Regarding proteases (ASP protease, papain), separation and quantification was not possible in Comparative Example 1 because they had similar substrate activities.

Therefore, the overall activity is shown in the graph of FIG.

As shown in FIGS. 1 to 3, each encapsulated enzyme A and B in the shampoo according to Example 1.

C maintains a high activity rate with almost no change in residual activity even after 100 days at both room temperature and 40°C, whereas the enzyme in the shampoo of Comparative Example 1 At both room temperature and 40'C, a rapid decrease in activity rate is shown in a short period of time.

In addition, in Example 1, three types of enzymes were blended, but
Since they are all encapsulated enzymes A, B, and C, the particle size and coloring of each encapsulated enzyme can be changed.
Top 4 middle B request ear Lφ? h Four consecutive bamboo sticks are extremely effective.

In addition, in Example 1, since the enzyme is encapsulated,
Enzyme-to-enzyme contact can be prevented, and the enzyme itself can be prevented from reacting as a substrate.

[Example 2] In this example, the present invention was applied to a rinse.

Rinse is after using shampoo as a cleaning agent.
By using it on degreased hair, the conditioner ingredients are adsorbed onto the hair and form a film that protects the hair and gives it shine and emollient effect.It also has an antistatic effect that makes it easier to style the hair. It gives flexibility to

Therefore, it is preferable to include a proteolytic enzyme or the like as the enzyme component.

Therefore, in this example, encapsulated protease A is blended into the rinse composition.

In addition, the effect menu of the encapsulated enzyme in this example;
The Torayasu 61819 lure was the same as Example 2, but without encapsulating the enzyme. However, the titer in the products is the same. ) were prepared, and the component compositions of both are shown in Table 2.

Table 2 Encapsulated enzyme A is a water-insoluble capsule.

The graph of FIG. 4 shows the changes over time in the enzyme activity rates in Example 2 and Comparative Example 2. Note that the storage conditions for the product for measuring changes over time to examine enzyme activity stability were room temperature and 40°C.

As shown in FIG. 4, the encapsulated enzyme A in the rinse according to Example 2 shows almost no change in residual activity even after 100 days at both room temperature and 40'C, and has high activity. On the other hand, the enzyme in the rinse of Comparative Example 2 shows a rapid decrease in activity rate in a short period of time at both room temperature and 40°C.

[Example 3] This example is a case where the present invention is applied to bath additives (liquid).

Bath additives are intended to enhance the effects of bathing, such as keeping warm, promoting blood circulation, and cleaning the whole body; therefore, the enzyme components include proteolytic enzymes, lipolytic enzymes, etc. It is preferable to mix them.

Therefore, in this example, encapsulated proteases A and B and encapsulated lipolytic enzyme C are blended into the bath additive composition.

In addition, in order to compare the effect of the encapsulated enzyme in this example with that of conventional bath additives, Comparative Example 3 (composition is the same as Example 3, but the enzyme is not encapsulated. Table 3 shows the component compositions of both.

Table 3 Changes in the enzyme activity rate over time in Example 3 and Comparative Example 3 are shown in the graphs of FIGS. 5 to 7.

Note that the storage conditions for the product for measuring changes over time to examine enzyme activity stability were room temperature and 40°C. In addition, regarding proteases (ASP protease, papain), since they have similar substrate activities, in Comparative Example 3,
Separation and quantification was not possible.

Therefore, the overall activity is shown in the graph of FIG.

As shown in FIGS. 5 to 7, each of the encapsulated enzymes A, B, and C in the bath additive according to Example 3 was heated at room temperature and at 40°C.
℃, there is almost no change in the residual activity rate even after 100 days, and the enzyme in the bath additive of Comparative Example 3 maintains a high activity rate at room temperature and 40℃.
Each of these shows a rapid decrease in activity rate over a short period of time.

In addition, in Example 3, three types of enzymes were blended, but
Since they are all encapsulated enzymes A, B, and C, by changing the particle size and coloring of each encapsulated enzyme, separation and quantification becomes possible, which is extremely effective for quality control.

In addition, in Example 3, since the enzyme is encapsulated,
Enzyme-to-enzyme contact can be prevented, and therefore, the enzyme itself can be prevented from reacting as a substrate.

[Example 4] This example is a case where the present invention was applied to a cleansing cream.

Cleansing creams are intended to cleanse the skin by removing dirt from the skin and makeup products that have been used on the face. Therefore, it is preferable to incorporate proteolytic enzymes, lipolytic enzymes, etc. as enzyme components.

In this example, encapsulated proteolytic enzymes A and B and encapsulated lipolytic enzyme C are blended into the cleansing cream composition.

In addition, in order to compare the effect of the encapsulated enzyme in this example with that of a conventional cleansing cream, Comparative Example 4 (the composition was the same as in Example 4, but the enzyme was not fully encapsulated. Table 4 shows the component compositions of both.

Table 4 It is a cell.

Changes in the enzyme activity rate over time in Example 4 and Comparative Example 4 are shown in the graphs of FIGS. 8 to 10.

Note that the storage conditions for the product for measuring changes over time to examine enzyme activity stability were room temperature and 40°C. In addition, as for proteases (ASP protease, papain), since they have similar substrate activities, in Comparative Example 4,
Separation and quantification was not possible.

Therefore, the overall activity is shown in the graph of FIG.

As shown in FIGS. 8 to 10, each encapsulated enzyme A, B, and C in the cleansing cream according to Example 4
The enzyme in the cleansing cream of Comparative Example 4 maintains a high activity rate with almost no change in residual activity even after 100 days at both room temperature and 40°C. At room temperature and 40℃,
It shows a rapid decrease in activity rate over a short period of time.

In addition, in Example 4, three types of enzymes were blended, but
Since they are all encapsulated enzymes A, B, and C, phantom separation V can be achieved by changing the particle size and coloring of each encapsulated enzyme.
The amount can be controlled and quality control is extremely effective.

In addition, in Example 4, since the enzyme is encapsulated,
Enzyme-to-enzyme contact can be prevented, and therefore, the enzyme itself can be prevented from reacting as a substrate.

[Example 5] This example is a case where the present invention is applied to a carpet cleaning liquid.

Carpet cleaning liquids are intended to remove dust and dirt adhering to carpets, but lipids and proteins are particularly difficult to remove. Therefore, it is preferable to incorporate proteolytic enzymes, lipolytic enzymes, etc. as enzyme components.

Therefore, in this example, encapsulated protease A and encapsulated lipolytic enzyme B are blended into the carpet cleaning liquid composition.

In addition, in order to compare the effect of the encapsulated enzyme in this example with that of a conventional carpet cleaning solution, Comparative Example 5 (composition is the same as in Example 5, but the enzyme is not encapsulated. The values are the same.)
Table 5 shows the component compositions of both.

Table 5 Encapsulated enzyme A, I: ij, water-soluble capsule.

Changes in the enzyme activity rate over time in Example 5 and Comparative Example 5 are shown in the graphs of FIGS. 11 and 12.

Note that the storage conditions for the product for measuring changes over time to examine enzyme activity stability were room temperature and 40°C.

As shown in FIGS. 11 and 12, each of the encapsulated enzymes A and B in the carpet cleaning solution according to Example 5 was
At both room temperature and 40°C, there is almost no change in the residual activity rate even after 100 days, and the enzyme in the carpet cleaning solution of Comparative Example 5 maintains a high activity rate. At each temperature of 40°C, a rapid decrease in activity rate is shown in a short period of time.

In addition, in Example 5, since the enzyme is encapsulated,
Enzyme-to-enzyme contact can be prevented, and therefore, the enzyme itself can be prevented from reacting as a substrate.

[Brief explanation of drawings]

FIGS. 1 to 3 show Sani/jl+ according to Embodiment 1 of the present invention.
-Control CI /r11n IK t? -+ 14
1 寥y;M pu;IT-J4':I2 J-Sk
A comparison table of the enzyme residual activity rate over time of the JIZ/7'l-Jh-Tally product, and FIG. 4 shows the enzyme residual activity rate over time of the sanitary product according to Example 2 of the present invention and the conventional Sanita IJ-11. Comparison table of enzyme residual activity rate of products over time,
Figures 5 to 7 are comparison tables of the enzyme residual activity rate over time of the sanitary product according to Example 3 of the present invention and the enzyme residual activity rate over time of conventional sanitary products, and Figures 8 to 10 are A comparison table of the enzyme residual activity rate over time of the sanitary product according to Example 4 of the present invention and the enzyme residual activity rate over time of the conventional sanitary product, FIGS. 11 and 12 are Example 5 of the present invention
2 is a comparison table of the enzyme residual activity rate over time of the sanitary product related to the above and the enzyme residual activity rate over time of the conventional sanitary product. Patent applicant Tadao Shiraishi

Claims (1)

[Claims] 1. An encapsulated enzyme-containing sanitary product characterized by containing an encapsulated enzyme in a base.