JPS62195288A - Production enhancement of microbial flocculant - Google Patents
Production enhancement of microbial flocculantInfo
- Publication number
- JPS62195288A JPS62195288A JP61036022A JP3602286A JPS62195288A JP S62195288 A JPS62195288 A JP S62195288A JP 61036022 A JP61036022 A JP 61036022A JP 3602286 A JP3602286 A JP 3602286A JP S62195288 A JPS62195288 A JP S62195288A
- Authority
- JP
- Japan
- Prior art keywords
- flocculant
- organic nitrogen
- microbial flocculant
- microorganism
- aeration
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 230000000813 microbial effect Effects 0.000 title claims description 9
- 238000004519 manufacturing process Methods 0.000 title claims description 8
- 125000001477 organic nitrogen group Chemical group 0.000 claims abstract description 10
- 244000005700 microbiome Species 0.000 claims abstract description 8
- 229940041514 candida albicans extract Drugs 0.000 claims abstract description 7
- 239000012138 yeast extract Substances 0.000 claims abstract description 7
- 238000000034 method Methods 0.000 claims abstract description 6
- 230000007935 neutral effect Effects 0.000 claims abstract description 6
- 239000001888 Peptone Substances 0.000 claims abstract description 5
- 108010080698 Peptones Proteins 0.000 claims abstract description 5
- 241000316848 Rhodococcus <scale insect> Species 0.000 claims abstract description 5
- 239000002253 acid Substances 0.000 claims abstract description 5
- 235000019319 peptone Nutrition 0.000 claims abstract description 5
- 238000005273 aeration Methods 0.000 claims description 15
- 150000007513 acids Chemical class 0.000 claims description 4
- 238000012258 culturing Methods 0.000 claims description 4
- 230000016615 flocculation Effects 0.000 claims description 4
- 238000005189 flocculation Methods 0.000 claims description 4
- 239000000126 substance Substances 0.000 claims description 4
- 230000003311 flocculating effect Effects 0.000 abstract description 4
- 235000015097 nutrients Nutrition 0.000 abstract description 2
- 241000187561 Rhodococcus erythropolis Species 0.000 abstract 1
- 239000012531 culture fluid Substances 0.000 abstract 1
- 230000000694 effects Effects 0.000 description 16
- 239000000243 solution Substances 0.000 description 12
- 238000004220 aggregation Methods 0.000 description 11
- 230000002776 aggregation Effects 0.000 description 11
- 239000002609 medium Substances 0.000 description 11
- 239000008394 flocculating agent Substances 0.000 description 6
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 4
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 4
- 229910052799 carbon Inorganic materials 0.000 description 4
- 239000005995 Aluminium silicate Substances 0.000 description 3
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 description 3
- 238000002835 absorbance Methods 0.000 description 3
- 235000012211 aluminium silicate Nutrition 0.000 description 3
- 239000004202 carbamide Substances 0.000 description 3
- 210000000641 erythrophore Anatomy 0.000 description 3
- NLYAJNPCOHFWQQ-UHFFFAOYSA-N kaolin Chemical compound O.O.O=[Al]O[Si](=O)O[Si](=O)O[Al]=O NLYAJNPCOHFWQQ-UHFFFAOYSA-N 0.000 description 3
- 239000010802 sludge Substances 0.000 description 3
- 239000006228 supernatant Substances 0.000 description 3
- 238000009423 ventilation Methods 0.000 description 3
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 2
- 239000007836 KH2PO4 Substances 0.000 description 2
- 241000187654 Nocardia Species 0.000 description 2
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 description 2
- 229910052921 ammonium sulfate Inorganic materials 0.000 description 2
- 235000011130 ammonium sulphate Nutrition 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 230000002708 enhancing effect Effects 0.000 description 2
- 239000008103 glucose Substances 0.000 description 2
- 239000001963 growth medium Substances 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 229910000402 monopotassium phosphate Inorganic materials 0.000 description 2
- 235000019796 monopotassium phosphate Nutrition 0.000 description 2
- 229910052757 nitrogen Inorganic materials 0.000 description 2
- GNSKLFRGEWLPPA-UHFFFAOYSA-M potassium dihydrogen phosphate Chemical compound [K+].OP(O)([O-])=O GNSKLFRGEWLPPA-UHFFFAOYSA-M 0.000 description 2
- 238000003756 stirring Methods 0.000 description 2
- 239000000758 substrate Substances 0.000 description 2
- 229920001059 synthetic polymer Polymers 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 description 1
- 229930091371 Fructose Natural products 0.000 description 1
- 239000005715 Fructose Substances 0.000 description 1
- RFSUNEUAIZKAJO-ARQDHWQXSA-N Fructose Chemical compound OC[C@H]1O[C@](O)(CO)[C@@H](O)[C@@H]1O RFSUNEUAIZKAJO-ARQDHWQXSA-N 0.000 description 1
- 230000000996 additive effect Effects 0.000 description 1
- 230000004520 agglutination Effects 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- 238000011143 downstream manufacturing Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- -1 etc. Chemical compound 0.000 description 1
- 239000013028 medium composition Substances 0.000 description 1
- 239000004006 olive oil Substances 0.000 description 1
- 235000008390 olive oil Nutrition 0.000 description 1
- 229920002401 polyacrylamide Polymers 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- 239000012085 test solution Substances 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
- 238000004065 wastewater treatment Methods 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Landscapes
- Treatment Of Sludge (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Separation Of Suspended Particles By Flocculating Agents (AREA)
Abstract
Description
【発明の詳細な説明】
〔技術分野〕
本発明はロードコツカス・エリスロポレス（旧名ノカル
ディア・エリスロポレス）にＲ−Ｓ−Ｉ株（ＦＥＲＭ３
５３０号）に代表されるように凝集剤生産能力を有する
ロードコツカス属微生物を培養して、微生物凝集剤を製
造するに際し、炭素源、有機体窒素源を選択し、かつ初
発ｐＨを中性からアルカリ性領域にし、過度な通気を抑
えて培養することにより。[Detailed Description of the Invention] [Technical Field] The present invention relates to the development of R-S-I strain (FERM3
When manufacturing a microbial flocculant by culturing Rhodococcoccus microorganisms that have the ability to produce flocculants, as typified by No. 530, carbon sources and organic nitrogen sources are selected, and the initial pH is changed from neutral to alkaline. By culturing in a separate area and suppressing excessive aeration.
微生物凝集剤の生産を増強する方法に関するものである
。The present invention relates to a method for enhancing the production of microbial flocculants.
従来、活性汚泥処理、土木浚渫処理剤として無機系凝集
剤及び合成高分子凝集剤が使用されてきた。しかしなが
ら１合成高分子凝集剤の中には、能力、経済性の面から
優れているが、ポリアクリルアミド系統のように安全性
及び二次公害の面からみて問題点が指摘されているのも
現実である。Conventionally, inorganic flocculants and synthetic polymer flocculants have been used as activated sludge treatment and civil engineering dredging treatment agents. However, although some synthetic polymer flocculants are superior in terms of performance and economy, the reality is that problems have been pointed out from the standpoint of safety and secondary pollution, such as the polyacrylamide series. It is.
これらの問題点を克服・解消し、環境保全の面からも、
安全性の面からも、新規な凝集剤の開発は廃水処理分野
のみならずあらゆる分野から切望されており、ダウンス
トリームプロセッシングの面からもその重要性は認識さ
れてきている。By overcoming and eliminating these problems, and from the perspective of environmental conservation,
From the standpoint of safety, the development of new flocculants is strongly desired not only in the wastewater treatment field but in all other fields, and its importance is also being recognized from the standpoint of downstream processing.
そこで、本発明者らは、すでに凝集効果が大きくかつ毒
性のない微生物凝集剤Ｎ０Ｃ−１（特許番号１０９６０
６２）の開発を行っており、今回、本凝集剤のより効率
的な生産増強方法について検討を進めたところ、培地の
栄養源を選択することにより、また培養ｐＨ１通気量を
選定することにより、極めて効果的に本凝集剤を生産増
強することを認め、本発明を完成するに至ったものであ
る。Therefore, the present inventors developed a microbial flocculant N0C-1 (Patent No. 10960), which has a large flocculating effect and is not toxic.
62), and we have now investigated a more efficient production enhancement method for this flocculant, and found that by selecting the nutrient source of the culture medium and the aeration rate at culture pH 1, It was recognized that production of this flocculant can be increased extremely effectively, and the present invention was completed.
本発明によれば、ロードコツカス属に属し、凝集能力を
有する微生物を培養して微生物凝集剤を製造するに際し
、酵母エキス、カザミノ酸及びペプトンの中から選ばれ
る少なくとも１種を含む含有物を有機窒素源として添加
し、初発ｐＨを中性からアルカリ性領域にし、かつ培養
液に対する通気比を１以下に制限して通気することを特
徴とする微生物凝集剤の生産増強法が提供される。According to the present invention, when producing a microbial flocculant by culturing a microorganism belonging to the genus Rhodococchus and having flocculating ability, organic nitrogen is added to the content containing at least one selected from yeast extract, casamino acids, and peptone. Provided is a method for enhancing the production of a microbial flocculant, which is characterized in that the initial pH is adjusted from neutral to alkaline, and the aeration ratio to the culture solution is limited to 1 or less for aeration.
本発明に使用される菌株は、ロードコツカス属に属し、
汚泥物質等に対し凝集能を有する菌株であればいずれで
もよいが、その代表例示菌株として、ロードコツカス・
エリスロポレス（旧名 ノカルディア・エリスロポレス
）（ＦＥＲＭ ３５３０号）が寄託されている。The strain used in the present invention belongs to the genus Rhodococchus,
Any strain may be used as long as it has the ability to flocculate sludge materials, etc., but as a representative example, Rhodococcus spp.
Erythrophores (formerly Nocardia erythrophores) (FERM No. 3530) has been deposited.
なお、旧名ノカルディア・エリスロポレスは１９８０年
に国際微生物命名規約委員会により、ロードコツカス・
エリスロポレスと再整理分類されている。The former name Nocardia erythropores was renamed to Rhodococcuscus in 1980 by the International Committee on Nomenclature of Microorganisms.
It has been reclassified as Erythrophores.
ところで、本凝集剤質を生産させる培地としては、これ
ら凝集能を有する微生物が生育できる培地であればどの
ような組成であっても使用できる。By the way, as a medium for producing the present flocculant substance, any composition can be used as long as it is a medium in which these microorganisms having flocculation ability can grow.
一般的な組成要素としては、炭素源及び硫酸アンモニウ
ム、尿素等の無機窒素源、その他、ＫＨ２ＰＯ４、Ｍｇ
ＳＯ４、ＣａＣＱ ２等の無機塩類等を含む培地が使用
されるが、望ましくは以下に示す培地組成、培養条件で
培養するのが好ましい。Common compositional elements include carbon sources, ammonium sulfate, inorganic nitrogen sources such as urea, etc., KH2PO4, Mg
A medium containing inorganic salts such as SO4 and CaCQ2 is used, and it is preferable to culture under the following medium composition and culture conditions.
炭素源においては、水溶性炭素源を用いる場合には、高
生育量を示すグルコース、フラクトース等の基質を用い
ることにより高凝集活性が得られ、かつ菌の生育と凝集
活性との間に比例関係が認められる。一方、非水溶性で
あるオリーブオイルを基質に使用すると培体量に比して
凝集活性は高くはない。無機窒素源では硫安、尿素が凝
集活性に良い、有機窒素源においては、酵母エキス、カ
ザミノ酸、ペプトンにより顕著な添加効果が認められる
。この他、至適可°養°幼発ｐｉは、７〜１１の中性か
らアルカリ性、好ましくは８．５〜９．５の弱アルカリ
性領域が適している。また、至適培養温度は３０℃が好
ましいが、２５℃、３７℃においても微生物は充分な生
育と凝集活性を示す、また１通気量は、培養液に対する
通気比で１以下、好ましくは０〜゛０．８の範囲に制限
した方が培地当りの凝集活性が高いことが判明した。こ
れらの結果を総合すると従来法に比べて８倍の活性を培
地当り増強させることが可能になった。When using a water-soluble carbon source as a carbon source, high aggregation activity can be obtained by using a substrate such as glucose or fructose that exhibits a high growth rate, and there is a proportional relationship between bacterial growth and aggregation activity. is recognized. On the other hand, when water-insoluble olive oil is used as a substrate, the aggregation activity is not high compared to the amount of culture medium. Among inorganic nitrogen sources, ammonium sulfate and urea are good for flocculation activity, and among organic nitrogen sources, yeast extract, casamino acids, and peptone have a remarkable additive effect. In addition, the optimum culturable infantile pi is in the neutral to alkaline range of 7 to 11, preferably in the slightly alkaline range of 8.5 to 9.5. In addition, although the optimum culture temperature is preferably 30°C, microorganisms exhibit sufficient growth and aggregation activity even at 25°C and 37°C, and the aeration rate is 1 or less, preferably 0 to 1, as the aeration ratio to the culture solution. It was found that the aggregation activity per medium was higher when the concentration was limited to a range of 0.8. Taking these results together, it became possible to increase the activity per medium by eight times compared to the conventional method.
なお１本明細書における培養液に対する通気比は、次の
式で表わされるものである。Note that the aeration ratio to the culture solution in this specification is expressed by the following formula.
Ｒ＝通気比
Ｖ、＝１分間当りの常圧空気の通気量（Ｑ）Ｖ２＝培養
液の容量（１１）
ところで、微生物産生凝集剤の被凝集対象は、活性汚泥
等の有機性からカオリン等の無機性まで広範囲にわたる
が、凝集力価測定法においては。R = aeration ratio V, = aeration rate of normal pressure air per minute (Q) V2 = volume of culture solution (11) By the way, the targets to be flocculated by the microorganism-produced flocculant range from organic substances such as activated sludge to kaolin, etc. Although it covers a wide range of inorganic properties, in the agglutination titer method.
被凝集物質としてカオリンを選定して行った。活性は上
澄液の透明度を１／濁度で表示した。即ち、１００ｍｇ
メスシリンダーに培養液（物）を０．５ｓｆｌ入れ、蒸
留水で１ＯＩＩＩＱにフィールアップした後、５０００
ρρｍのカオリン懸濁液８０■Ｑと１０％塩化カルシウ
ム塩１０■悲を加えて攪拌後、５分後の上澄液の吸光度
を波長５５０ｎｍにおいて測定する。凝集活性は供試液
を用いた時の上澄の吸光度の逆数から、対照の吸光度の
逆数を差し引いた値として算出し力価とした。即ち、こ
の逆数の値が大きいほど透明度が大きいことを意味し、
凝集活性が高いことを示す。Kaolin was selected as the substance to be agglomerated. The activity was expressed as the transparency of the supernatant liquid divided by the turbidity. i.e. 100mg
Put 0.5sfl of culture solution (material) into a graduated cylinder, increase the volume to 1OIIIQ with distilled water, and then add 5000
After adding 80 μm of a kaolin suspension of ρρm and 10 μm of a 10% calcium chloride salt and stirring, the absorbance of the supernatant liquid after 5 minutes was measured at a wavelength of 550 nm. The aggregation activity was calculated as the value obtained by subtracting the reciprocal of the absorbance of the control from the reciprocal of the absorbance of the supernatant when the test solution was used, and the titer was determined. In other words, the larger the value of this reciprocal, the greater the transparency.
Indicates high aggregation activity.
次に、実施例によって本発明を更に詳しく説明する。 Next, the present invention will be explained in more detail with reference to Examples.
実施例１
グルコース１％、尿素０．０５％、Ｋ２ＨＰＯ４０，５
％。Example 1 Glucose 1%, Urea 0.05%, K2HPO40.5
%.
ＫＨ２ＰＯ４０，２％、Ｍｇ５０４０．０２％の培地に
酵母エキス等の有機体窒素源を０．０５％添加した培地
ｌＯ抛Ｑを５００■ａの三角フラスコに入れ、 ｐＨ７
，５に調整した後、１２０℃、１５分間加圧減菌した後
、ロードコツカス・エリスロポレス（旧名ノカルディア
・エリスロホＬ／ ス）ＫＲ−５７株−テＦ’ＥＲＭ
３５３０号） ｔｒ、 接ｍ シ、３０℃にて培養し、
各培養液の凝集活性の経時変化を調べた。A medium containing 0.2% KH2PO4 and 0.02% Mg50 and 0.05% organic nitrogen source such as yeast extract was added to a 500-a Erlenmeyer flask, and the pH was 7.
, 5, and sterilized under pressure at 120°C for 15 minutes.
3530), cultured at 30°C,
Changes in the aggregation activity of each culture solution over time were investigated.
各種有機体窒素源添加による培養液０．５ｍ！当りの最
大凝集活性を表−１に示す。0.5m culture solution with addition of various organic nitrogen sources! The maximum aggregation activity per unit is shown in Table-1.
これらの結果より、有機体窒素源として酵母エキス、カ
ザミノ酸、ペプトンを添加することにより培養液の凝集
活性は明らかに増大することが判明した。These results revealed that the flocculation activity of the culture solution was clearly increased by adding yeast extract, casamino acids, and peptone as organic nitrogen sources.
表−１
実施例２
実施例１の培地に有機体窒素源としての酵母エキスを０
．０５％添加した培地１００ｓ ｍを５００−〇の三角
フラスコに入れ、培地の初発ｐＨを６．０．７．５．８
．５．９．５に調整した後、１２０℃、１５分間加圧滅
菌した後、ロードコツカス・エリスロポレスにＲ−Ｓ
−［（ＦＥＲＭ３５３０号）を接種し、３０℃にて培養
し、培養液の凝集活性の経時変化を調べた。初発ｐＨと
各培養該０．５ｍＱ当りの最大凝集活性値を表−２に示
す。Table 1 Example 2 Yeast extract as an organic nitrogen source was added to the medium of Example 1.
．． Pour 100 s m of medium supplemented with 0.5% into a 500-〇 Erlenmeyer flask, and adjust the initial pH of the medium to 6.0.7.5.8.
．． After adjusting to 5.9.5 and autoclaving at 120°C for 15 minutes, R-S
-[(FERM No. 3530) was inoculated and cultured at 30°C, and changes over time in the aggregation activity of the culture solution were examined. Table 2 shows the initial pH and the maximum aggregation activity value per 0.5 mQ of each culture.
さらに、培地の初発ｐＨをｐＨ１０，１１にしても本菌
は生育し、培養液は凝集活性をもつ。Furthermore, even if the initial pH of the medium is set to pH 10 or 11, the bacteria grow and the culture solution has flocculating activity.
以上の結果より、培養液の初発ｐＨを中性からアルカリ
性領域にするのが凝集剤質生産には望ましいことが判明
した。From the above results, it has been found that it is desirable for the production of flocculant to keep the initial pH of the culture solution from neutral to alkaline.
表−２
実施例３
実施例２に示す培地（ｐＨ８，５）２ Ｑを卓上型ジャ
ーファーメーター（丸菱製、５Ｑ容器）の培養槽に入れ
、１２０℃、１５分間加圧滅菌後、ロードコツカス・エ
リスロポレスにＲ−５−Ｉ株（ＦＥＲＭ ３５３０号）
を接種し、３０℃にて毎秒５００回転の攪拌数にて通気
攪拌培養した。空気の通気量はゼロ（通気比：０）、１
／ｗｉｎ（通気比＝１）、４ Ｑ ／ｗｉｎ（通気比：
２）の３段階に設定した。各通気比において得られた培
養液０．５ｔａＱ当りの最大凝集力を表−３に示す。Table 2 Example 3 The medium (pH 8,5) 2Q shown in Example 2 was placed in the culture tank of a tabletop Jarfer meter (manufactured by Marubishi, 5Q container), and after autoclaving at 120°C for 15 minutes, Rhodococcus spp.・Erythropores strain R-5-I (FERM No. 3530)
was inoculated and cultured with aeration at 30° C. at a stirring speed of 500 revolutions per second. Air ventilation amount is zero (airflow ratio: 0), 1
/win (ventilation ratio = 1), 4 Q /win (ventilation ratio:
2) was set in three stages. Table 3 shows the maximum cohesive force per 0.5 taQ of culture solution obtained at each aeration ratio.
この結果、過度の通気量は高活性凝集剤生産には好まし
くなく、通気比が１以下になるように空気の通気量を制
限して培養するのが望ましいことが判明した。As a result, it was found that an excessive amount of aeration is not preferable for producing a highly active flocculant, and that it is desirable to limit the amount of air aeration and culture so that the aeration ratio is 1 or less.
表−３
〔効 果〕
以上の実験結果から、本発明によれば、微生物凝集剤を
効率よく生産することができる。Table 3 [Effect] From the above experimental results, according to the present invention, a microbial flocculant can be efficiently produced.
Claims (1)
物を培養して微生物凝集剤を製造するに際し、酵母エキ
ス、カザミノ酸及びペプトンの中から選ばれる少なくと
も１種を含む含有物を有機窒素源として添加し、初発ｐ
Ｈを中性からアルカリ性領域にし、かつ培養液に対する
通気比を１以下に制限して通気することを特徴とする微
生物凝集剤の生産増強法。(1) When producing a microbial flocculant by culturing a microorganism that belongs to the genus Rhodococcus and has flocculation ability, a substance containing at least one selected from yeast extract, casamino acids, and peptone is used as an organic nitrogen source. Added, initial p.
1. A method for increasing the production of a microbial flocculant, which comprises changing H from a neutral to alkaline range and aerating the culture solution by limiting the aeration ratio to 1 or less.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP61036022A JPH0661268B2 (en) | 1986-02-20 | 1986-02-20 | Microbial flocculant production enhancement method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP61036022A JPH0661268B2 (en) | 1986-02-20 | 1986-02-20 | Microbial flocculant production enhancement method |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS62195288A true JPS62195288A (en) | 1987-08-28 |
| JPH0661268B2 JPH0661268B2 (en) | 1994-08-17 |
Family
ID=12458099
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP61036022A Expired - Lifetime JPH0661268B2 (en) | 1986-02-20 | 1986-02-20 | Microbial flocculant production enhancement method |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPH0661268B2 (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH01211492A (en) * | 1988-02-18 | 1989-08-24 | Agency Of Ind Science & Technol | Enhanced production of microorganism flocculant noc-1 |
| CN110104921A (en) * | 2019-04-09 | 2019-08-09 | 浙江工商大学 | A method of adding microbial fermentation solution improves waste activated sludge dewatering |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS5186189A (en) * | 1974-12-25 | 1976-07-28 | Ajinomoto Kk | BISEIBUTSUNYORUTANPAKUGYOSHUKATSUSEIBUTSUSHITSUNO SEIZOHO |
| JPS5639633A (en) * | 1979-09-06 | 1981-04-15 | Nippon Gakki Seizo Kk | Loop filter circuit in phase-locked loop circuit |
| JPS58183910A (en) * | 1982-04-22 | 1983-10-27 | Agency Of Ind Science & Technol | Flocculation of filthy substance by microorganism |
-
1986
- 1986-02-20 JP JP61036022A patent/JPH0661268B2/en not_active Expired - Lifetime
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS5186189A (en) * | 1974-12-25 | 1976-07-28 | Ajinomoto Kk | BISEIBUTSUNYORUTANPAKUGYOSHUKATSUSEIBUTSUSHITSUNO SEIZOHO |
| JPS5639633A (en) * | 1979-09-06 | 1981-04-15 | Nippon Gakki Seizo Kk | Loop filter circuit in phase-locked loop circuit |
| JPS58183910A (en) * | 1982-04-22 | 1983-10-27 | Agency Of Ind Science & Technol | Flocculation of filthy substance by microorganism |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH01211492A (en) * | 1988-02-18 | 1989-08-24 | Agency Of Ind Science & Technol | Enhanced production of microorganism flocculant noc-1 |
| CN110104921A (en) * | 2019-04-09 | 2019-08-09 | 浙江工商大学 | A method of adding microbial fermentation solution improves waste activated sludge dewatering |
Also Published As
| Publication number | Publication date |
|---|---|
| JPH0661268B2 (en) | 1994-08-17 |
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