CN107118965A - A kind of marine active sludge product and preparation method thereof and water treatment applications - Google Patents
A kind of marine active sludge product and preparation method thereof and water treatment applications Download PDFInfo
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
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- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/02—Aerobic processes
- C02F3/12—Activated sludge processes
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
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- C02F3/28—Anaerobic digestion processes
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
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- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2209/00—Controlling or monitoring parameters in water treatment
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Abstract
The present invention relates to a kind of marine active sludge product and preparation method thereof and water treatment applications, the marine active sludge product is rich in resistance to, halophilic microorganism from ocean or other salt environments, exist with the cotton-shaped activated sludge form of particle or the form for separating composite bacteria agent, DO contents in product be 0.1~4.0mg/L, environment temperature be 0~50 degree, salinity be that 2~34%, pH value is 5~9, and with the activated sludge form in the presence of, its SVI is that 50~200mL/g, MLVSS are 1.2~7.5g/L.The preparation method that the present invention is provided, innovatively halophilic microorganism culture technique and activated sludge culture technological incorporation are used, microbial cleaning system new, for high salt treatment of Organic Wastewater is prepared, the runtime of high wastewater degradation ability, economic operating cost and temperature is realized, and it is widely applicable.Present invention also offers related method for treating water, the water treatment capacity and application surface of marine active sludge product are improved, practicality is stronger.
Description
Technical field
The present invention relates to microorganism water treatment field, and in particular to a kind of marine active sludge product and preparation method thereof and
Water treatment applications.
Background technology
High salt organic waste water is one of industrial water body difficult to deal with present, due in waste water containing a large amount of inorganic salts from
Son, causes high salt organic waste water to be difficult to be degraded by microorganisms, and uses the cost of physics, chemical treatment method higher, therefore seeks
Efficient biologic treating technique is sought to purify high salt organic waste water be a green technology problem.
Describe in the prior art " a kind of method of enhanced biological Anaerobic Treatment high salt organic wastewater with high concentration "（Application number
CN201610198750.3）, by reactor design and using microbial acclimation technology, salinity can be handled below 5g/L's
High salt organic waste water；Because the salinity of industrial high salt organic waste water is often higher than 30g/L, this method is difficult to for handling work
High salt organic waste water in industry, it is also difficult to apply to processing ocean organic wastewater（The average salinity of world ocean is 3.5%）, it is real
It is not strong with property.
Describe in the prior art " a kind of anaerobic ammonia oxidation reactor operation method for handling high-salt wastewater "（Application number
CN201410095868.4）, by using the quick acclimatization technology of salinity, realize the salt tolerant energy for stepping up microbe colony
Power, can handle high salt organic waste water of the salinity in below 35g/L；But the salt resistance ability for the microbe colony that this method is used has
Limit, is not suitable for the treatment of Organic Wastewater of higher salinity, and domestication process is uncontrollable, is used according to different types of high-salt wastewater
Domestication process be also required to change, applicable surface is wide.
" colonization method of floating stuffing in a kind of high-salt wastewater MBBR processing systems " described in prior art（Application number
CN201510580959.1）, by adding halophilic microorganism microbial inoculum, and adjusting ambient conditional parameter into reactor, promote thermophilic
Salt microbe colony biofilm is attached to filler surface, forms halophilic microorganism ecological environment, has preferably to high salt organic waste water
Degradation capability；But the ecosystem for the halophilic microorganism microbial inoculum that this method is used is excessively single, culture difficulty is larger, applicable surface
It is wideless.
Therefore, highly efficient high salt organic waste water biologic treating technique is sought, it is micro- by using the salt tolerant more optimized
Bioecosystem, improves microorganism to the degradation capability of high salt concentration organic wastewater, and can be suitably used for that a variety of industry are organic to be contained
There is the widespread demand of waste water, be the problem of being currently needed for solving.
The content of the invention
For defect of the prior art, present invention aims at provide a kind of marine active sludge product and its preparation side
Method and water treatment applications, by directly turning out new activated sludge product from ooze, sea water mixing thing（I.e. microorganism gives birth to
State system）, for the processing of a variety of high salt organic waste waters, realize microorganism water process efficiently, practical.
To achieve the above object, the technical scheme that provides of the present invention is：
In a first aspect, the invention provides a kind of marine active sludge product, it is characterised in that：The marine active sludge product
Rich in resistance to, halophilic microorganism from ocean or other salt environments, the marine active sludge product is with the cotton-shaped activity of particle
Sludge form or the form presence for separating composite bacteria agent, the dissolved oxygen of the marine active sludge product（DO）Content be 0.1~
4.0mg/L（It is anaerobic environment less than 0.5mg/L）, environment temperature be 0~50 degree（Long-term storage is preferably 4 degree）, salinity be 2
~34%, pH value be 5~9, and with the activated sludge form in the presence of, its sludge volume index（SVI）For 50~200mL/g,
Mixed liquor volatile suspended solid, MLVSS concentration（MLVSS）For 1.2~7.5g/ L.
Activated sludge process is a kind of the most frequently used bioremediation, and operation price is extremely low, micro- life that degrading waste water is used
Thing is the microbial ecosystem survived based on floc sludge carrier.In the present invention, marine active sludge i.e. from ooze, seawater or
Resistance to, the halophilic microorganism colony being separately cultured in other salt environments, wherein solid-state carrier are mainly that original ooze and resistance to, thermophilic salt are micro-
The mixture of the biological organism macromolecular of biological production, in order to meet the different application modes such as industry, research, the present invention is carried
The marine active sludge product of confession exists with the form of the cotton-shaped activated sludge form of particle or separation composite bacteria agent.It should also say
It is bright, according to compound resistance to, halophilic microorganism colony species the difference of customization, the dissolving in the marine active sludge product
Oxygen（DO）Content is 0.1~0.5mg/L（For anaerobism is resistance to, halophilic microorganism）, 0.5~4.0mg/L（For aerobic resistance to, thermophilic salt
Microorganism）Or 0.1~4.0mg/L（For the facultative resistance to, halophilic microorganism of good anaerobism）；The environment temperature of storage is preferably 4 degree,
But various based on marine microorganism flora species, it is all feasible to be preserved under 0~50 degree, is set with species；According to should
Water body salinity, pH value are different, and the salinity oriented in the marine active sludge product of production is preferably 2~34%,
PH value is preferably 5~9；In addition, in the presence of the marine active sludge product is with activated sludge form, in order to prevent sludge swollen
Swollen or bag card product optimal sludge concentration, its sludge volume index（SVI）Preferably 50~200mL/g, mixed liquor volatility
Suspended sediment concentration（MLVSS）Preferably 1.2~7.5g/ L, this scope is also allowed for dispensing and preserved simultaneously.
That is, a kind of marine active sludge product that the present invention is provided, by the way that activated sludge process and resistance to, thermophilic salt is micro-
Biodegradation technique joint operation, produces marine active sludge product, realizes traditional active sludge in high salt organic waste water
Application in biological treatment, the scope of application is wider.
Second aspect, the invention provides a kind of preparation method of marine active sludge product, it is characterised in that bag
Include following steps：The ooze that do not sterilize is taken as microorganism growing carrier, seawater and configures nutrient solution；Cultivated in bioreactor
30~180 days, obtain marine active sludge；The living environment for sexually revising marine active sludge is pointed to, dominant microflora is enriched with；By sea
Foreign activated sludge is dispensed by product specification, or separation obtains composite bacteria agent.
In the further embodiment of the present invention, the initial sludge volume index of the ooze（SVI）For 5~35mL/
G, the salinity of the seawater is that the element ratio of C, N, P in 2~34%, the nutrient solution of configuration are preferably 100：5：1, its correspondence
COD（COD）Concentration is 100~600mg/L.It should be noted that the initial sludge volume index of the ooze
（SVI）, salinity depend on sample point, the SVI of the ooze in general area is relatively low, between 5~20mL/g, and pollution or nutrient compared with
The marine site of horn of plenty, the SVI of the ooze of sampling is higher, can reach 20~35mL/g；The average salinity in whole world ocean is 3.5%,
Ooze, the seawater salinity of general offshore area sampling are 2~3.5%, and some extreme body of salt environment such as Dead Seas, up to 34 %
（2011 annual datas）, the salinity in the Dead Sea and the waters that nearby crosses is then between 3.5~34%；C, N, P in the nutrient solution of configuration
Element ratio 100：5：It is optimal culture condition when 1, changing C, N, P ratio can have a certain impact to microbiologic population, C,
N, P ratio are during follow-up post-directed training or domestication with requiring to change；Its correspondence COD（COD）If concentration is less than
100mg/L can cause nutrition not enough, the nutrient that microbial ecosystem cannot be sufficient, cause cultivation cycle length or culture to be lost
Lose, and COD exceedes the demand of culture microorganism if higher than 600mg/L, superior microorganism growth is too fast, and the ecosystem is unstable
It is fixed, while also result in the waste of culture medium.
During cultivate the marine active sludge, the nutrient solution for cultivating marine active sludge renews number of times
For 2~7 times/week, the dissolved oxygen of culture environment（DO）Content is 0.1~4.0mg/L（It is Anaerobic culturel less than 0.5mg/L）, training
The temperature for supporting environment is 20~40 degree.It should be noted that culture activated sludge process needs to renew nutrient solution by cycle timing
To ensure that microorganism has the nutrient growth of abundance, in Initial stage of culture, renew number of times and should be once a day（I.e. 7 times/week）, progressively drop
Low to 2 times/week extremely cultures are completed；And the DO contents of culture environment are set according to needed for training objective thalline, Anaerobic culturel is used as
When, DO contents be 0.1~0.5mg/L, during as aerobic culture, DO contents be 0.5~4.0mg/L, for good anaerobism it is facultative it is resistance to,
The thermophilic micro- life of salt can use DO for 0.1~4.0mg/L, but good, the micro- life of anaerobism salt ratio in the DO contents influence ecosystem,
Controlled according to actual customization；Simultaneously because the suitable living environment of marine microorganism is at 20~40 degree, therefore the temperature of culture environment
Degree is preferably in this temperature range, and speed is cultivated in otherwise influence.
In the further embodiment of the present invention, the living environment that the sensing sexually revises marine active sludge is mainly wrapped
Organic components, inorganic ion composition, salinity, pH value, temperature, dissolved oxygen amount, the mixing speed changed in culture solution is included,
Pass through the corresponding dominant microflora of the Environmental enrichment for changing the ecosystem.Marine active is sexually revised it should be noted that pointing to
The living environment of sludge is the process of a similar domestication, is enriched with the dominant bacteria in marine active sludge by customizing, and is used
To handle the high salt organic waste water under respective conditions, directive property parameter includes changing the organic components in culture solution, that is, worked as
Use other organic matters, the organic matter of particularly toxic property（Such as phenol, polythene derivative）During as microbe carbon source,
Being only capable of explaining the marine microorganism of these organic matters can survive, by the culture of certain time, in marine active sludge then
It is enriched the microorganism species for the organic components or derivatives thereof that can degrade；For inorganic ion composition, when in water body
Contain other inorganic salts, the organic matter of particularly toxic property（Such as mercury ion, chromium ion, nitrite ion）When, it is only capable of
Being resistant to the marine microorganism of these inorganic ions can survive, by the culture of certain time, in marine active sludge then
It is enriched the microorganism species for being resistant to these inorganic ions；As one kind of inorganic ion composition, work as salinity（Refer in particular to
Sodium chloride）During change, then the microorganism species that can be survived under specific salinity are then enriched in marine active sludge；For pH
Value, although most of marine microorganism is suitable for relative neutral environment（PH value is 6.5 ~ 8.5）, wherein be also no lack of it is various it is acidproof,
Alkaline-resisting marine microorganism, so that the pH value of condition water body environment, then rich in marine active sludge after the culture of certain time
The microorganism species that can be survived under specific pH are collected；For temperature, the influence due to temperature to cytoactive is different because of strain,
So being enriched with the dominant microflora in marine active sludge at a certain temperature, be conducive to improving the applicable surface of marine active sludge,
But because most of marine microorganism can survive in the environment of 5 ~ 45 degree, influence of the temperature to microorganism with respect to other factors its
Effect is smaller；For dissolved oxygen amount（DO）, during as Anaerobic culturel, DO contents are 0.1~0.5mg/L, during as aerobic culture, DO
Content is 0.5~4.0mg/L, and DO can be used for 0.1~4.0mg/L for facultative resistance to, the thermophilic micro- life of salt of good anaerobism, therefore DO contains
Good, the micro- life of anaerobism salt ratio in the amount influence ecosystem；And mixing speed mainly influences the knot between microorganism and activated sludge
Close, the big microorganism of stickiness can be more attached on activated sludge, the more nutriment due to activated sludge absorption is then used
During larger mixing speed, after the culture of certain time, stickiness larger microorganism species in part in marine active sludge
Ratio can increase.
In the further embodiment of the present invention, the packing specification of the marine active sludge is 50~200mL/g's
Sludge volume index（SVI）, 1.2~7.5g/ L mixed liquor volatile suspended solid, MLVSS concentration（MLVSS）, the separation process
The suspension bacteria liquid obtained by sieving desliming, centrifugal filtration and concentration.It should be noted that the packing rule of marine active sludge
Lattice determine shelf-life and the cost performance of product, and when the SVI of the marine active sludge after packing is less than 50mL/g, its activity is too
It is low, micro organism quantity very little, when the marine active sludge after packing SVI be higher than 200mL/g when, due to partial sludge expansion,
When largely using the particle suspensions of bioreactor can be caused to be difficult to settle, so as to influence water treatment efficiency；When MLVSS contents
Less than 1.2g/L, very little, corresponding water process effect is then inadequate for the amount of the marine active sludge of packing, and when MLVSS contents are high
When 7.5g/L, the amount of the marine active sludge of packing is too many, then microbe number therein is also a lot, due in unit volume
Not enough, therefore MLVSS is too high can cause the shortened shelf life of product for the nutriment that retains.
A kind of preparation method for marine active sludge product that the present invention is provided, first by halophilic microorganism culture technique and
Activated sludge culture technological incorporation is used, and has prepared microbial cleaning system new, for high salt treatment of Organic Wastewater
System, realizes the runtime of high wastewater degradation ability, economic operating cost and temperature, and widely applicable.
The third aspect, present invention also offers a kind of water treatment applications method of marine active sludge product, is used for
Low salt concn（Salinity is 2~10%）During wastewater degradation, for the ecosystem modifying agent of the biological water treatment system of existing high salt
When, the marine active sludge product is added directly into bioreactor；For the biological water treatment system of newly-built high salt
During original flora, the marine active sludge product is individually carried out to activation in 3~7 days and expanded after culture, life is added in batches
In thing reactor.It should be noted that because the salinity of target waste water is not high, the accessible existence of marine microorganism energy is simultaneously numerous
Grow in target waste water, and obtain efficient degradation rate；It is certain due to having in reactor when being applied as the modifying agent
Microflora basis, the marine active sludge product of addition is intended merely to add new resistance to, Halophiles to ecosystem system
Group, can directly add；When the original flora in as New bioreactor, the marine active sludge product of addition is preferably single
Solely carry out activation in 3~7 days and expand culture, then be added in batches in reactor, with the increase marine microorganism compared with limits
Bacterium amount, prevents excessive environmental impact in water treatment procedure from causing microorganism to be difficult to amount reproduction and extending bioreactor
The startup time is, it is necessary to which additional description, because marine microorganism can be enriched with breeding for 2 ~ 3 days under optimum conditions, then described
Activation expands incubation time and should be greater than 3 days, and excess resource and time were then wasted more than 7 days, it is not necessary that.
In addition, when marine active sludge be used for salinity be 10~34% and the above superelevation salt biological treatment system when, Ying Jiang
The marine active sludge product individually carries out activation in 3~7 days and expands culture, and life is added in batches again after carrying out classification domestication
In thing reactor.It should be noted that to prevent excessive environmental impact in water treatment procedure from causing microorganism to be difficult to largely
Breed and influence the water treatment efficiency of bioreactor, then need to carry out activation expansion culture（Similarly need 3 ~ 7 days）；Again due to salt
Height is spent, more than the tolerance range of half marine microorganism, only small part marine microorganism can survive and breed described
In superelevation salt biological treatment system, it is therefore desirable to classification domestication（The classification is domesticated for water process term, improves not with referring to gradient
The envirment factor grown beneficial to microorganism, to improve the tolerance of microorganism）It is added in batches in bioreactor again afterwards, with
Improve the salt resistance ability of marine microorganism.
The method for treating water for the marine active sludge product that the present invention is provided, by combining water process experimental technology, is improved
The water treatment capacity and application surface of marine active sludge product, practicality are stronger.
The additional aspect and advantage of the present invention will be set forth in part in the description, and will partly become from the following description
Obtain substantially, or recognized by the practice of the present invention.
Brief description of the drawings
Fig. 1 be the embodiment of the present invention in the marine active sludge product preparation method flow chart.
Fig. 2 is to prepare the equipment schematic diagram of marine active sludge product in the embodiment of the present invention.
Fig. 3 handles height at different temperatures for the marine active sludge product prepared in the embodiment of the present invention two, three and four
The COD degradation rate of salt organic waste water.
Fig. 4 handles high salt for the marine active sludge product prepared in the embodiment of the present invention two, three and four under different pH
The COD degradation rate of organic wastewater.
Fig. 5 locates for the marine active sludge product prepared in the embodiment of the present invention two, three and four under different mixing speeds
Manage the COD degradation rate of high salt organic waste water.
Fig. 6 is the dominant bacteria isolated in the marine active sludge product for preparing in the embodiment of the present invention two, three and four
Bacterium colony photo on solid medium.
Fig. 7 be in the embodiment of the present invention five the marine active sludge product for preparing under different salinity or phenol initial amount
The ability of degradation of phenol.
Embodiment
Below in conjunction with the accompanying drawing in the embodiment of the present invention, the technical scheme in the embodiment of the present invention is carried out clear, complete
Site preparation is described, it is clear that described embodiment is only a part of embodiment of the invention, rather than whole embodiments.It is based on
Embodiment in the present invention, it is every other that those of ordinary skill in the art are obtained under the premise of creative work is not made
Embodiment, belongs to the scope of protection of the invention.
Embodiment one
The present embodiment provides a kind of marine active sludge product, it is characterised in that：The marine active sludge product is rich in and is derived from
Ocean or resistance to, the halophilic microorganism of other salt environments, the marine active sludge product is with the cotton-shaped activated sludge form of particle
Or the form of separation composite bacteria agent is present, the dissolved oxygen of the marine active sludge product（DO）Content is 0.1~4.0mg/L
（It is anaerobic environment less than 0.5mg/L）, environment temperature be 0~50 degree（Long-term storage is preferably 4 degree）, salinity be 2~34%,
PH value be 5~9, and with the activated sludge form in the presence of, its sludge volume index（SVI）For 50~200mL/g, mixed liquor
VSS concentration（MLVSS）For 1.2~7.5g/ L.
For the marine active sludge product of the present invention, the present invention devises the preparation side of the marine active sludge product
Method.
Fig. 1 be the embodiment of the present invention in the marine active sludge product preparation method flow chart, as shown in figure 1,
The preparation method for the marine active sludge product that the present embodiment is provided, it is characterised in that comprise the following steps：
S1：The ooze that do not sterilize is taken as microorganism growing carrier, seawater and configures nutrient solution；
S2：Cultivated 30~180 days in bioreactor, obtain marine active sludge；
S3：The living environment for sexually revising marine active sludge is pointed to, dominant microflora is enriched with；
S4：Marine active sludge is dispensed by product specification, or separation obtains composite bacteria agent.
The equipment such as Fig. 2 used in the preparation method of the marine active sludge product provided with the present invention, incubation
It is shown：Fig. 2 mainly includes material storing box and culture to prepare the equipment schematic diagram of marine active sludge product in the embodiment of the present invention
Case, nutrient medium of the material storing box to configure culture marine active sludge, incubator is loading and culture marine active sludge.
It should be noted that activated sludge process is a kind of the most frequently used bioremediation, operation price is extremely low, and degraded is useless
The microorganism that water is used is the microbial ecosystem survived based on floc sludge carrier.In the present invention, marine active sludge is
Resistance to, the halophilic microorganism colony being separately cultured from ooze, seawater or other salt environments, wherein solid-state carrier are mainly original sea
The mixture of mud and resistance to, halophilic microorganism production biological organism macromolecular, in order to meet the different applications such as industry, research
Mode, the marine active sludge product that the present invention is provided is with the cotton-shaped activated sludge form of particle or the form of separation composite bacteria agent
In the presence of.It should also be noted that according to compound resistance to, halophilic microorganism colony species the difference of customization, the marine active is dirty
DO contents in mud product are 0.1~0.5mg/L（For anaerobism is resistance to, halophilic microorganism）, 0.5~4.0mg/L（For aerobic
Resistance to, halophilic microorganism）Or 0.1~4.0mg/L（For the facultative resistance to, halophilic microorganism of good anaerobism）；The environment temperature of storage is excellent
Elect 4 degree as, but it is various based on marine microorganism flora species, and it is all feasible to be preserved under 0~50 degree, is set with species
It is fixed；Different according to the water body salinity of application, pH value, the salinity oriented in the marine active sludge product of production is preferred
For 2~34%, pH value is preferably 5~9；In addition, in the presence of the marine active sludge product is with activated sludge form, in order to
Prevent the optimal sludge concentration of sludge bulking or bag card product, its SVI be preferably 50~200mL/g, MLVSS be preferably 1.2~
7.5g/ L, this scope is also allowed for dispensing and preserved simultaneously.
The preparation method of marine active sludge product with reference to specific embodiment to being provided in the embodiment of the present invention is made
Further illustrate.
Embodiment two
Present embodiments provide a kind of preparation method of marine active sludge product, it is characterised in that comprise the following steps：
S1：The golden unpasteurized ooze in bay outdoor bathing place in Weihai is taken as microorganism growing carrier, the golden bay in Weihai outdoor bathing place is taken not
The seawater configuration nutrient solution of sterilizing, the initial sludge volume index of ooze（SVI）For 12mL/g, the extra large body environment of the sampling
Salinity is 2.8%, and the nutrient solution of configuration includes glucose 0.21g/L, starch 0.17g/L, ammonium chloride 0.06g/L, phosphoric acid
Potassium dihydrogen 0.003g/L（C, N, P element ratio are about 100：5：1）, its correspondence COD（COD）Concentration is 400mg/L；
S2：Cultivated 90 days in bioreactor, obtain marine active sludge；During the marine active sludge is cultivated
Do not stir, nutrient solution renew number of times for first 30 days 7 times/week, in 30 days 4 times/week, latter 30 days 2 times/week, culture environment it is molten
Solve oxygen（DO）Content is 2.0mg/L, and the temperature of culture environment is 30 degree；
S3：The living environment for sexually revising marine active sludge is pointed to, dominant microflora is enriched with；During being somebody's turn to do, glucose is changed to equivalent
Sucrose（Organic components）, ammonium chloride be changed to equivalent ammonium nitrate（Inorganic salts ingredients）, salinity increase to 5%, pH value adjust to 7,
Temperature adjustment to 25 degree, dissolved oxygen amount regulation arrives 3.0mg/L, adds 100rmp magnetic agitation；
S4：Marine active sludge is dispensed by product specification；The packing specification of the marine active sludge for 100mL/g SVI,
2.5g/ L MLVSS.
Embodiment three
Present embodiments provide a kind of preparation method of marine active sludge product, it is characterised in that comprise the following steps：
S1：The unpasteurized ooze in Bohai Sea Area is taken as microorganism growing carrier, the unpasteurized seawater in Huanghai Sea area is taken and matches somebody with somebody
Put nutrient solution, the initial sludge volume index of ooze（SVI）For 35mL/g, the salinity of the extra large body environment of the sampling is 2%, is matched somebody with somebody
The nutrient solution put includes sucrose 0.05g/L, peptone 0.05g/L, ammonium nitrate 0.015g/L, dipotassium hydrogen phosphate
0.001g/L（Its correspondence COD concentration is about 100mg/L）；
S2：Cultivated 30 days in bioreactor, obtain marine active sludge；During the marine active sludge is cultivated
Do not stir, nutrient solution renew number of times for first 10 days 7 times/week, in 10 days 5 times/week, latter 10 days 3 times/week, the DO of culture environment
Content is 0.1mg/L, and the temperature of culture environment is 40 degree；
S3：The living environment for sexually revising marine active sludge is pointed to, dominant microflora is enriched with；During being somebody's turn to do, sucrose is changed to equivalent breast
Sugar, peptone are changed to equivalent milk powder（Organic components）, dipotassium hydrogen phosphate be changed to equivalent potassium chloride（Inorganic salts ingredients）, salinity
Increase to 7%, pH value and adjust the magnetic agitation that 1.0mg/L, addition 150rmp are arrived to 6, temperature adjustment to 20 degree, dissolved oxygen amount regulation；
S4：Marine active sludge is dispensed by product specification；The packing specification of the marine active sludge for 200mL/g SVI,
7.5g/ L MLVSS.
Example IV
Present embodiments provide a kind of preparation method of marine active sludge product, it is characterised in that comprise the following steps：
S1：The unpasteurized ooze in the Dead Sea is taken as microorganism growing carrier, the unpasteurized seawater configuration nutrient solution in the Dead Sea is taken,
The initial SVI of ooze is 5mL/g, and the salinity of the extra large body environment of the sampling is 34%, and the nutrient solution of configuration includes glucose
0.31g/L, starch 0.27g/L, ammonium chloride 0.06g/L, potassium dihydrogen phosphate 0.003g/L（Its correspondence COD concentration is 600mg/
L）；
S2：Cultivated 120 days in bioreactor, obtain marine active sludge；Cultivating the process of the marine active sludge
In do not stir, nutrient solution renew number of times for first 30 days 7 times/week, in 60 days 5 times/week, latter 30 days 4 times/week, culture environment
DO contents are 4.0mg/L, and the temperature of culture environment is 20 degree；
S3：The living environment for sexually revising marine active sludge is pointed to, dominant microflora is enriched with；During being somebody's turn to do, glucose is changed to equivalent
Galactolipin（Organic components）, ammonium chloride be changed to equivalent ammonium nitrate（Inorganic salts ingredients）, salinity be reduced to 20%, pH value regulation
2.0mg/L, addition 80rmp magnetic agitation are arrived to 8, temperature adjustment to 25 degree, dissolved oxygen amount regulation；
S4：The packing specification of the marine active sludge not dispensed is 50mL/g SVI, 1.2g/ L MLVSS, by ocean
Activated sludge passes through sieving desliming, centrifugal filtration and concentration, the suspension bacteria liquid of acquisition（Composite bacteria agent）.
For the water treatment capacity that the obtained marine active of research is dirty, mud is according to above-described embodiment two, embodiment three, embodiment
The four marine active sludge products prepared, have carried out water treatment capacity measure：
In contrast experiment, the amount of the marine active sludge used is 100L（Before example IV is extracted using composite bacteria agent
Marine active sludge）, SVI is 70mL/g, and MLVSS is 3g/ L；The salinity of the pending high salt organic waste water used is 5%,
And contain glucose 0.21g/L, starch 0.17g/L, ammonium chloride 0.06g/L, potassium dihydrogen phosphate 0.003g/L, its correspondence COD
Concentration is 400mg/L；Water process determines the COD degradation rate in water body after 12 hours（Assay method is permanganate method：GB/
T11892-1989）, the change of experiment parameter includes environment temperature（20~45 degree）、pH（3~11）And mixing speed（60~
220rpm）, the convenient running environment of marine active sludge is determined by changing environmental condition.
Fig. 3 is that the marine active sludge product for preparing is at different temperatures in the embodiment of the present invention two, three and four（PH value is consolidated
It is set to 7, without stirring）Handle the COD degradation rate of high salt organic waste water.As shown in figure 3, under 20~45 degree, prepared by embodiment two
The COD clearances of marine active sludge be more than the COD clearances of embodiment three, four, embodiment three is minimum, and at 35 degree
When, the COD clearance highests of each embodiment, more than 80%.Illustrate that the bacterium in marine active sludge can be under 20~45 degree
The biodegradability for showing the microflora in biological degradation activity, and the marine active sludge of embodiment two is greater than reality
Apply example three, four（Embodiment three is minimum）；It also illustrate that the activated sludge product turned out under different condition has difference simultaneously
Microbial ecosystem, and its sensitivity to temperature differs.
Fig. 4 be in the embodiment of the present invention two, three and four the marine active sludge product for preparing under different pH（Temperature is fixed
For 25 degree, without stirring）Handle the COD degradation rate of high salt organic waste water.As shown in figure 4, under pH3~11, prepared by embodiment two
The COD clearances of marine active sludge be more than the COD clearances of embodiment three, four, embodiment three is when pH is 3,5 or 11
Minimum, example IV is minimum when pH is 7~9, and pH be 7 when, the COD clearance highests of each embodiment, 80% with
On.Illustrate that the bacterium in marine active sludge can show biological degradation activity, and the generally sea of embodiment two under pH3~11
The biodegradability of microflora in foreign activated sludge is greater than embodiment three, four；And in inclined marine environment pH conditions
Under（PH7~9）, the marine active that the biodegradability of the marine active sludge performance of embodiment three is higher than example IV is dirty
Mud, illustrates that the marine active sludge of embodiment three bioactivity in the range of this pH is higher, but in other pH value, embodiment three
The COD clearances of marine active sludge decline to a great extent, and the biodegradability of the marine active sludge of example IV performance
It is higher than the marine active sludge of embodiment three, the anti-pH of the marine active sludge of this explanation example IV（Soda acid）Changing capability
Outline is strong；It also illustrate that the activated sludge product turned out under different condition differs to pH sensitivity above.
Fig. 5 be in the embodiment of the present invention two, three and four the marine active sludge product for preparing under different mixing speeds（Temperature
Degree be fixed as 25 degree, pH be fixed as 7）Handle the COD degradation rate of high salt organic waste water.As shown in figure 5, stirring in 60~220rpm
Mix under speed, the COD clearances of marine active sludge prepared by embodiment two are more than the COD clearances of embodiment three, four, implement
Example three is minimum, and in 200rpm mixing speed, the COD clearance highests of each embodiment, more than 75%, explanation is stirred
Speed is mixed to have a certain impact to the COD clearances of each embodiment；But COD clearance of each embodiment under different mixing speeds
Change trend is almost identical, and it is the processing speed of macrosystem to illustrate what different mixing speeds mainly influenceed（Waterpower cycle, micro- life
Thing attachment ratio, concentration of suspended particles）, it is little to microcosmic microbial ecological capacity.
For the preliminary difference for confirming the strain in above example marine active sludge product, its dominant bacteria is by albumen
Peptone-Solid agar culture separation, Fig. 6 is in the marine active sludge product prepared in the embodiment of the present invention two, three and four point
Bacterium colony photo of the dominant bacteria separated out on solid medium, according to Fig. 6, marine active sludge prepared by embodiment two
In dominant bacteria show yellow color colonies on culture medium, the dominant bacteria in marine active sludge prepared by embodiment three is in training
Support and milky bacterium colony is shown on base, the dominant bacteria in marine active sludge prepared by example IV shows yellowish green on culture medium
Color bacterium colony, intuitively these dominant bacterias of surface are not a kind of, and further third party's detection data represent the yellow color colonies
Strain be that albumen Pseudomonas, the strain of the milky white bacterium colony are that Bacillus, the strain of the milky white bacterium colony are vibrio.
In the implementation of the present invention, the parameter for cultivating activated sludge is extremely important, should illustrate for step S1
It is that the initial SVI of the ooze is preferably 5~35mL/g, during the salinity of the seawater is 2~34%, the nutrient solution of configuration
C, N, P element ratio are preferably 100：5：1, its correspondence COD concentration is 100~600mg/L；The initial SVI of the ooze, salinity
Depending on sample point, the SVI of the ooze in general area is relatively low, between 5~20mL/g, and in the sea of pollution or nutrient compared with horn of plenty
Domain, the SVI of the ooze of sampling is higher, can reach 20~35mL/g；The average salinity in whole world ocean is 3.5%, general coastal waters
Ooze, the seawater salinity of area's sampling are 2~3.5%, and some extreme body of salt environment such as Dead Seas, up to 34 %（2011 years
According to）, the salinity in the Dead Sea and the waters that nearby crosses is then between 3.5~34%；C, N, P element ratio in the nutrient solution of configuration
100：5：It is optimal culture condition when 1, changing C, N, P ratio can have a certain impact to microbiologic population, and C, N, P ratio exist
With requiring to change during follow-up post-directed training or domestication；If its correspondence COD concentration can cause nutrition not enough less than 100mg/L,
Microbial ecosystem cannot get sufficient nutrient, cause cultivation cycle length or culture failure, and COD is if higher than if 600mg/L
More than the demand of culture microorganism, superior microorganism growth is too fast, and the ecosystem is unstable, while also result in the wave of culture medium
Take.
In the further implementation process for cultivating the marine active sludge, should be noted that for step S2 is used for
The number of times that renews for cultivating the nutrient solution of marine active sludge is 2~7 times/week, and the DO contents of culture environment are 0.1~4.0mg/L
（It is Anaerobic culturel less than 0.5mg/L）, the temperature of culture environment is 20~40 degree；Culture activated sludge process needs fixed by the cycle
Shi Huanxin nutrient solutions, in Initial stage of culture, renew number of times and should be 1 times/day, progressively drop to ensure that microorganism has the nutrient growth of abundance
Low to 2 times/week extremely cultures are completed；And the DO contents of culture environment are set according to needed for training objective thalline, Anaerobic culturel is used as
When, DO contents be 0.1~0.5mg/L, during as aerobic culture, DO contents be 0.5~4.0mg/L, for good anaerobism it is facultative it is resistance to,
The thermophilic micro- life of salt can use DO for 0.1~4.0mg/L, but good, the micro- life of anaerobism salt ratio in the DO contents influence ecosystem,
Controlled according to actual customization；Simultaneously because the suitable living environment of marine microorganism is at 20~40 degree, therefore the temperature of culture environment
Degree is preferably in this temperature range, and speed is cultivated in otherwise influence.
In the further implementation process for cultivating the marine active sludge, it should be noted that for step S3 described
The living environment for sexually revising marine active sludge is pointed to mainly including changing the organic components in culture solution, inorganic ion
Composition, salinity, pH value, temperature, dissolved oxygen amount, mixing speed, it is corresponding described excellent by the Environmental enrichment for changing the ecosystem
Gesture flora；Wherein, the living environment that sensing sexually revises marine active sludge is the process of a similar domestication, by customizing sea
Dominant bacteria enrichment in foreign activated sludge, to handle the high salt organic waste water under respective conditions, directive property parameter includes changing
The organic components become in culture solution, i.e., when using other organic matters, the organic matter of particularly toxic property（Such as phenol, poly-
Ethene derivatives etc.）During as microbe carbon source, being only capable of explaining the marine microorganism of these organic matters can survive, and pass through one
The microbial bacteria for the organic components or derivatives thereof that can degrade then is enriched in the culture fixed time, marine active sludge
Group；For inorganic ion composition, when containing other inorganic salts, the organic matter of particularly toxic property in water body（Such as mercury ion,
Chromium ion, nitrite ion etc.）When, being only resistant to the marine microorganism of these inorganic ions can survive, and pass through one
The microorganism species for being resistant to these inorganic ions are then enriched in the culture fixed time, marine active sludge；As inorganic
One kind of salt ion composition, works as salinity（Refer in particular to sodium chloride）During change, then being then enriched in marine active sludge to survive
Microorganism species under specific salinity；For pH value, although most of marine microorganism is suitable for relative neutral environment（PH value
For 6.5 ~ 8.5）, wherein also it is no lack of various acidproof, alkaline-resisting marine microorganisms, so that the pH value of condition water body environment, by certain
The microorganism species that can be survived under specific pH are then enriched after the culture of time, in marine active sludge；For temperature, due to
Influence of the temperature to cytoactive is different because of strain, so the dominant microflora in enrichment marine active sludge at a certain temperature,
Be conducive to improving the applicable surface of marine active sludge, but because most of marine microorganism can survive in the environment of 5 ~ 45 degree,
With respect to other factors, its effect is smaller for influence of the temperature to microorganism；For DO, during as Anaerobic culturel, DO contents be 0.1~
0.5mg/L, during as aerobic culture, DO contents are 0.5~4.0mg/L, and DO can be used for facultative resistance to, the thermophilic micro- life of salt of good anaerobism
For 0.1~4.0mg/L, therefore good, the micro- life of anaerobism salt ratio in the DO contents influence ecosystem；And the main shadow of mixing speed
The combination between microorganism and activated sludge is rung, the big microorganism of stickiness can be more attached on activated sludge, because activated sludge is inhaled
Attached more nutriment, then when using larger mixing speed, after the culture of certain time, in marine active sludge
The ratio of the larger microorganism species of part stickiness can increase.
In the further implementation process for cultivating the marine active sludge, it should be noted that for step S4 described
The packing specification of marine active sludge is 50~200mL/g SVI, 1.2~7.5g/ L MLVSS, and the separation process is to lead to
Cross the suspension bacteria liquid that sieving desliming, centrifugal filtration and concentration are obtained.It should be noted that the packing specification of marine active sludge is determined
Determine shelf-life and the cost performance of product, when the SVI of the marine active sludge after packing is less than 50mL/g, its activity is too low,
Micro organism quantity very little, when the marine active sludge after packing SVI be higher than 200mL/g when, due to partial sludge expansion, largely
When using the particle suspensions of bioreactor can be caused to be difficult to settle, so as to influence water treatment efficiency；When MLVSS contents are less than
1.2g/L, very little, corresponding water process effect is then inadequate for the amount of the marine active sludge of packing, and when MLVSS contents are higher than
During 7.5g/L, the amount of the marine active sludge of packing is too many, then microbe number therein is also a lot, due to unit volume internal memory
Not enough, therefore MLVSS is too high can cause the shortened shelf life of product for the nutriment that stays.
Pass through a kind of described above, marine active sludge product provided in an embodiment of the present invention, first by halophilic microorganism
Culture technique and activated sludge culture technological incorporation are used, prepared it is new, for the micro- of high salt treatment of Organic Wastewater
Biological purification system, realizes application of the traditional active sludge in high salt organic waste water biological treatment, has reached high waste water
The runtime of degradation capability, economic operating cost and temperature, practicality is higher.
In order to further expand the scope of application of marine active sludge product, the embodiments of the invention provide ocean work
The water treatment applications method of property sludge product, is described further with reference to specific embodiment to it.
Embodiment five
Present embodiments provide the preparation method and water treatment applications method of a kind of marine active sludge product, it is characterised in that
Comprise the following steps：
S1：The unpasteurized ooze in East China Sea is taken as microorganism growing carrier, the unpasteurized seawater in East China Sea is taken and matches somebody with somebody
Nutrient solution is put, the initial SVI of ooze is 20mL/g, and the salinity of the extra large body environment of the sampling is 3%, the nutrient solution of configuration
Include starch 0.2g/L, lactose 0.2g/L, ammonium chloride 0.03g/L, potassium dihydrogen phosphate 0.003g/L（Its correspondence COD concentration is about
For 400mg/L）；
S2：Cultivated 90 days in bioreactor, obtain marine active sludge；During the marine active sludge is cultivated
Do not stir, nutrient solution renew number of times for first 30 days 7 times/week, in 30 days 4 times/week, latter 30 days 2 times/week, the DO of culture environment
Content is 1mg/L, and the temperature of culture environment is 25 degree；
S3：The living environment for sexually revising marine active sludge is pointed to, dominant microflora is enriched with；During being somebody's turn to do, starch is changed to equivalent benzene
Phenol（Organic components）, potassium dihydrogen phosphate be changed to equivalent potassium chloride（Inorganic salts ingredients）, salinity increase to 3.5%, pH value regulation
2mg/L, addition 100rmp magnetic agitation are arrived to 7, temperature adjustment to 20 degree, dissolved oxygen amount regulation；
S4：Marine active sludge is dispensed by product specification；The packing specification of the marine active sludge for 100mL/g SVI,
2.5g/ L MLVSS.
S5：For salinity for 3.5~10% biological water treatment system of existing high salt the ecosystem modifying agent when, by institute
Marine active sludge product is stated to be added directly into bioreactor；The amount of the marine active sludge wherein used is
100L, the salinity of the pending high salt organic waste water used is 3.5~10%（Multiple-group analysis）, and contain phenol 1g/L；Water process
The phenol residual quantity in each group water body is determined after 12 hours；
S6：For salinity for the 2% biological water treatment system of newly-built high salt original flora when, the marine active sludge is produced
Product individually carry out activation in 3~7 days and expanded after culture, and point 3 equivalent are added in bioreactor；The ocean wherein used
The amount of activated sludge is 100L, and the salinity of the pending high salt organic waste water used is initially 2%（Add the ocean of salinity 3.5%
Salinity can be raised after activated sludge, between 2~3.5%）, and contain phenol 0,0.5,1.0,1.5,2.0g/L（Five groups of contrasts）；
Water process determines the phenol clearance in each group water body after 12 hours.
Fig. 7 be in the embodiment of the present invention five the marine active sludge product for preparing in different salinity（Step S5）Or phenol
Under initial amount（Step S6）The ability of degradation of phenol, as described in Figure 7, for step S5 experiment, when salinity rises since 3.5%
Gao Shi, the phenol disposal ability of marine active sludge slowly declines, but in salinity 6% and following experiment, residual phenol amount exists
In 2mg/L, and when salinity is more than 6.5% and during the above, the phenol degrading ability of marine active sludge drastically declines, it is impossible to use again
In the processing of the high salt phenol wastewater；For step S6 experiment, when phenol initial concentration is in 0~1g/L, marine active is dirty
The phenol disposal ability of mud is higher, close to 100%, but when phenol initial concentration is in more than 1g/L, the phenol of marine active sludge
Degradation capability drastically declines, and phenol removal ability deficiency is, it is necessary to rethink the design of technique.
It should be noted that the water treatment applications method of the marine active sludge product provided using the present embodiment,
For low salt concn（Salinity is 2~10%）During wastewater degradation, because the salinity of target waste water is not high, marine microorganism energy nothing
The existence of obstacle is simultaneously bred in target waste water, obtains efficient degradation rate；When being applied as the modifying agent, due to anti-
Answering has certain microflora basis in device, the marine active sludge product of addition is intended merely to the addition of ecosystem system newly
Resistance to, Halophiles group, can directly add；When the original flora in as New bioreactor, the marine active of addition is dirty
Mud product preferably individually carries out activation in 3~7 days and expands culture, then is added in batches in reactor, with the increase compared with limits
The bacterium amount of marine microorganism, prevents excessive environmental impact in water treatment procedure from causing microorganism to be difficult to amount reproduction and extend
Startup time of bioreactor, it is necessary to additional description, because marine microorganism can be enriched with for 2 ~ 3 days under optimum conditions
Breeding, then the activation expands incubation time and should be greater than 3 days, and excess resource and time were then wasted more than 7 days, it is not necessary that.
Embodiment six
Present embodiments provide the preparation method and water treatment applications method of a kind of marine active sludge product, it is characterised in that
Comprise the following steps：
S1：Take Qinghai Area（Salt lake, belongs to the hypersaline environment of non-ocean water body）Unpasteurized lake mud grows as microorganism
Carrier, takes the unpasteurized seawater configuration nutrient solution in the Nanhai area, the initial SVI of lake mud is 25mL/g, the extra large body of the sampling
The salinity of environment is 3.2%, the nutrient solution of configuration comprising glucose 0.3g/L, sucrose 0.2g/L, ammonium chloride 0.02g/L,
Potassium dihydrogen phosphate 0.002g/L（Its correspondence COD concentration is about 500mg/L）；
S2：Cultivated 80 days in bioreactor, obtain marine active sludge；During the marine active sludge is cultivated
Do not stir, nutrient solution renew number of times for first 30 days 7 times/week, in 30 days 3 times/week, latter 20 days 2 times/week, the DO of culture environment
Content is 0.5mg/L, and the temperature of culture environment is 20 degree；
S3：The living environment for sexually revising marine active sludge is pointed to, dominant microflora is enriched with；During being somebody's turn to do, sucrose is changed to equivalent milk
Powder（Organic components）, ammonium chloride be changed to equivalent potassium chloride（Inorganic salts ingredients）, salinity increase to 5%, pH value adjust to 7.5,
Temperature adjustment to 25 degree, dissolved oxygen amount regulation arrives 2.5mg/L, adds 50rmp magnetic agitation；
S4：Marine active sludge is dispensed by product specification；The packing specification of the marine active sludge for 80mL/g SVI,
3g/ L MLVSS；
S5：For salinity be 10% high salt biological treatment system when, by the marine active sludge product individually carry out work in 3 days
Change and expand culture, divide again 2 times after progress classification domestication and be added in bioreactor；The marine active sludge wherein used
Amount be 50L, 0.3g/L containing glucose, sucrose 0.2g/L；Water process determines the COD clearances in water body after 12 hours；
S6：For salinity be 34% superelevation salt biological treatment system when, by the marine active sludge product individually carry out 7 days
Activation expands culture, divides again 5 times after progress classification domestication and is added in bioreactor；The marine active wherein used is dirty
The amount of mud is 50L, 0.3g/L containing glucose, sucrose 0.2g/L；Water process determines the COD clearances in water body after 12 hours.
Measurement result shows, for the present embodiment step S5 experiment, due to salinity（10%）Adopted in only incubation step S3
Salinity（5%）Twice, the 12 hours COD clearances measured be 84 ± 5%, show the microorganism in the marine active sludge
Twice of salinity is adapted to, and has preferable wastewater degradation ability；And for the present embodiment step S6 experiment, due to salinity
（34%）For the salinity used in incubation step S3（5%）6.8 times, the 12 hours COD clearances measured are only 21 ± 7%, are shown
Microorganism in most of marine active sludge does not adapt to the salinity of superelevation, but still has that part is resistance to, halophilic microorganism performance
Go out activity, obtain certain COD clearances, the organic wastewater that this superelevation salinity is handled in theory is also feasible, but need to use
Longer treatment time of water or use instead it is more effectively cultivating, containing more resistance to, halophilic microorganism flora marine active sludge.
It should be noted that the classification is domesticated for water process term, improves with referring to gradient and be unfavorable for microorganism growth
Envirment factor, to improve the tolerance of microorganism.Meanwhile, in the water treatment procedure of the present embodiment, due to marine active sludge
For the superelevation salt biological treatment system that salinity is 10~34% and the above, the marine active sludge product should individually be carried out 3
Activation in~7 days expands culture, is added in batches in bioreactor again after carrying out classification domestication, and to prevent water-treated
Excessive environmental impact causes microorganism to be difficult to amount reproduction and influences the water treatment efficiency of bioreactor, it is necessary to carry out in journey
Activation expands culture（3 ~ 7 days）；Again due to too high in salinity, more than the tolerance range of half marine microorganism, only small part sea
Foreign microorganism can survive and breed in described superelevation salt biological treatment system, it is therefore desirable to add in batches again after classification domestication
Enter into bioreactor, to improve the salt resistance ability of marine microorganism.
The method for treating water for the marine active sludge product that the present invention is provided, by combining water process experimental technology, is improved
The water treatment capacity and application surface of marine active sludge product, practicality are stronger.
Although embodiments of the invention have been shown and described above, it is to be understood that above-described embodiment is example
Property, it is impossible to limitation of the present invention is interpreted as, one of ordinary skill in the art within the scope of the invention can be to above-mentioned
Embodiment is changed, changed, replacing and modification, and the essence of appropriate technical solution is departed from various embodiments of the present invention skill
The scope of art scheme, it all should cover among the claim of the present invention and the scope of specification.
Claims (8)
1. a kind of marine active sludge product, it is characterised in that：The marine active sludge product be rich in from ocean or other
Resistance to, the halophilic microorganism of salt environment, the marine active sludge product are compound with the cotton-shaped activated sludge form of particle or separation
The form of microbial inoculum is present, the dissolved oxygen of the marine active sludge product（DO）Content is 0.1~4.0mg/L（Less than 0.5mg/L
For anaerobic environment）, environment temperature be 0~50 degree（Long-term storage is preferably 4 degree）, salinity be that 2~34%, pH value is 5~9, and
In the presence of the activated sludge form, its sludge volume index（SVI）Suspended for 50~200mL/g, mixed liquor volatility solid
Bulk concentration（MLVSS）For 1.2~7.5g/ L.
2. a kind of preparation method of marine active sludge product according to claim 1, it is characterised in that including following step
Suddenly：
S1：The ooze that do not sterilize is taken as microorganism growing carrier, seawater and configures nutrient solution；
S2：Cultivated 30~180 days in bioreactor, obtain marine active sludge；
S3：The living environment for sexually revising marine active sludge is pointed to, dominant microflora is enriched with；
S4：Marine active sludge is dispensed by product specification, or separation obtains composite bacteria agent.
3. a kind of preparation method of marine active sludge product according to claim 2, it is characterised in that：The ooze
Initial sludge volume index（SVI）For 5~35mL/g, the salinity of the seawater be C in 2~34%, the nutrient solution of configuration,
N, P element ratio are preferably 100：5：1, its correspondence COD（COD）Concentration is 100~600mg/L.
4. a kind of preparation method of marine active sludge product according to claim 2, it is characterised in that：It is described in culture
During marine active sludge, the number of times that renews of nutrient solution is 2~7 times/week, the dissolved oxygen of culture environment（DO）Content is
0.1~4.0mg/L（It is Anaerobic culturel less than 0.5mg/L）, the temperature of culture environment is 20~40 degree.
5. a kind of preparation method of marine active sludge product according to claim 2, it is characterised in that：The directive property
Change marine active sludge living environment mainly include change culture solution in organic components, inorganic ion composition,
Salinity, pH value, temperature, dissolved oxygen amount, mixing speed, pass through the corresponding dominant bacteria of the Environmental enrichment for changing the ecosystem
Group.
6. a kind of preparation method of marine active sludge product according to claim 2, it is characterised in that：Live the ocean
Property sludge packing specification be 50~200mL/g sludge volume index（SVI）, 1.2~7.5g/ L mixed liquor volatility hang
Floating solid concentration（MLVSS）, the separation process is the suspension bacteria liquid obtained by sieving desliming, centrifugal filtration and concentration.
7. a kind of water treatment applications of marine active sludge product according to claim 1, it is characterised in that：For salinity
For 2~10% biological water treatment system of existing high salt the ecosystem modifying agent when, the marine active sludge product is direct
It is added in bioreactor；For salinity for 2~10% biological water treatment system of newly-built high salt original flora when, by institute
State marine active sludge product individually to carry out after activation expansion culture in 3~7 days, be added in batches in bioreactor.
8. a kind of water treatment applications of marine active sludge product according to claim 1, it is characterised in that：For salinity
For 10~34% and the above superelevation salt biological treatment system when, by the marine active sludge product individually carry out work in 3~7 days
Change and expand culture, be added in batches in bioreactor again after carrying out classification domestication.
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114276963A (en) * | 2022-01-04 | 2022-04-05 | 南昌航空大学 | Breeding method and application of deep-sea salt-tolerant phenol degrading bacteria |
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