JPS6146116B2 - - Google Patents
Info
- Publication number
- JPS6146116B2 JPS6146116B2 JP53045535A JP4553578A JPS6146116B2 JP S6146116 B2 JPS6146116 B2 JP S6146116B2 JP 53045535 A JP53045535 A JP 53045535A JP 4553578 A JP4553578 A JP 4553578A JP S6146116 B2 JPS6146116 B2 JP S6146116B2
- Authority
- JP
- Japan
- Prior art keywords
- hrp
- peroxidase
- enzyme
- activity
- hydrogen donor
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
- 102000003992 Peroxidases Human genes 0.000 claims description 35
- 230000000694 effects Effects 0.000 claims description 25
- 108040007629 peroxidase activity proteins Proteins 0.000 claims description 25
- 239000000852 hydrogen donor Substances 0.000 claims description 20
- 238000000034 method Methods 0.000 claims description 17
- 150000002978 peroxides Chemical class 0.000 claims description 12
- 239000000758 substrate Substances 0.000 claims description 12
- 238000006243 chemical reaction Methods 0.000 claims description 8
- 238000012921 fluorescence analysis Methods 0.000 claims description 7
- 238000006911 enzymatic reaction Methods 0.000 claims description 6
- 230000002378 acidificating effect Effects 0.000 claims description 5
- QRMZSPFSDQBLIX-UHFFFAOYSA-N homovanillic acid Chemical compound COC1=CC(CC(O)=O)=CC=C1O QRMZSPFSDQBLIX-UHFFFAOYSA-N 0.000 description 18
- 102000004190 Enzymes Human genes 0.000 description 13
- 108090000790 Enzymes Proteins 0.000 description 13
- 230000035945 sensitivity Effects 0.000 description 10
- 238000005259 measurement Methods 0.000 description 9
- 108700020962 Peroxidase Proteins 0.000 description 8
- 239000000243 solution Substances 0.000 description 7
- 239000002253 acid Substances 0.000 description 5
- 239000000126 substance Substances 0.000 description 5
- MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 description 4
- 239000007864 aqueous solution Substances 0.000 description 4
- 238000002360 preparation method Methods 0.000 description 4
- WDGFFVCWBZVLCE-UHFFFAOYSA-N purpurogallin Chemical group C1=CC=C(O)C(=O)C2=C1C=C(O)C(O)=C2O WDGFFVCWBZVLCE-UHFFFAOYSA-N 0.000 description 4
- 241000196324 Embryophyta Species 0.000 description 3
- 238000004847 absorption spectroscopy Methods 0.000 description 3
- 239000000427 antigen Substances 0.000 description 3
- 102000036639 antigens Human genes 0.000 description 3
- 108091007433 antigens Proteins 0.000 description 3
- 239000000872 buffer Substances 0.000 description 3
- -1 but among them Proteins 0.000 description 3
- 108010044467 Isoenzymes Proteins 0.000 description 2
- DZGWFCGJZKJUFP-UHFFFAOYSA-N Tyramine Natural products NCCC1=CC=C(O)C=C1 DZGWFCGJZKJUFP-UHFFFAOYSA-N 0.000 description 2
- 150000007513 acids Chemical class 0.000 description 2
- HVYWMOMLDIMFJA-DPAQBDIFSA-N cholesterol Chemical compound C1C=C2C[C@@H](O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)CCCC(C)C)[C@@]1(C)CC2 HVYWMOMLDIMFJA-DPAQBDIFSA-N 0.000 description 2
- 230000005284 excitation Effects 0.000 description 2
- 125000002485 formyl group Chemical class [H]C(*)=O 0.000 description 2
- 238000003018 immunoassay Methods 0.000 description 2
- IWDCLRJOBJJRNH-UHFFFAOYSA-N p-cresol Chemical compound CC1=CC=C(O)C=C1 IWDCLRJOBJJRNH-UHFFFAOYSA-N 0.000 description 2
- 238000011002 quantification Methods 0.000 description 2
- 229960003732 tyramine Drugs 0.000 description 2
- DZGWFCGJZKJUFP-UHFFFAOYSA-O tyraminium Chemical compound [NH3+]CCC1=CC=C(O)C=C1 DZGWFCGJZKJUFP-UHFFFAOYSA-O 0.000 description 2
- 235000011330 Armoracia rusticana Nutrition 0.000 description 1
- 240000003291 Armoracia rusticana Species 0.000 description 1
- 108091003079 Bovine Serum Albumin Proteins 0.000 description 1
- 102000008015 Hemeproteins Human genes 0.000 description 1
- 108010089792 Hemeproteins Proteins 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 102000004316 Oxidoreductases Human genes 0.000 description 1
- 108090000854 Oxidoreductases Proteins 0.000 description 1
- 239000012670 alkaline solution Substances 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 239000012491 analyte Substances 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 150000004982 aromatic amines Chemical class 0.000 description 1
- 238000012742 biochemical analysis Methods 0.000 description 1
- 229940098773 bovine serum albumin Drugs 0.000 description 1
- 238000011095 buffer preparation Methods 0.000 description 1
- 239000007853 buffer solution Substances 0.000 description 1
- 230000003139 buffering effect Effects 0.000 description 1
- 238000007385 chemical modification Methods 0.000 description 1
- 235000012000 cholesterol Nutrition 0.000 description 1
- 238000003759 clinical diagnosis Methods 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 239000000470 constituent Substances 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 229930003836 cresol Natural products 0.000 description 1
- 230000002255 enzymatic effect Effects 0.000 description 1
- 238000005558 fluorometry Methods 0.000 description 1
- 230000000984 immunochemical effect Effects 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 238000002372 labelling Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 230000007935 neutral effect Effects 0.000 description 1
- 150000007530 organic bases Chemical class 0.000 description 1
- 238000006395 oxidase reaction Methods 0.000 description 1
- 238000007254 oxidation reaction Methods 0.000 description 1
- 239000000813 peptide hormone Substances 0.000 description 1
- 229920000768 polyamine Polymers 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 239000011541 reaction mixture Substances 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 235000000346 sugar Nutrition 0.000 description 1
- 150000008163 sugars Chemical class 0.000 description 1
- 239000013076 target substance Substances 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
Landscapes
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP4553578A JPS54137397A (en) | 1978-04-18 | 1978-04-18 | Method of measuring activity of peroxidase |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP4553578A JPS54137397A (en) | 1978-04-18 | 1978-04-18 | Method of measuring activity of peroxidase |
Publications (2)
Publication Number | Publication Date |
---|---|
JPS54137397A JPS54137397A (en) | 1979-10-25 |
JPS6146116B2 true JPS6146116B2 (de) | 1986-10-13 |
Family
ID=12722076
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP4553578A Granted JPS54137397A (en) | 1978-04-18 | 1978-04-18 | Method of measuring activity of peroxidase |
Country Status (1)
Country | Link |
---|---|
JP (1) | JPS54137397A (de) |
Families Citing this family (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS60200167A (ja) * | 1984-03-24 | 1985-10-09 | Toyobo Co Ltd | 化学発光法による過酸化水素の定量法 |
Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS5852640A (ja) * | 1981-09-22 | 1983-03-28 | Toppan Printing Co Ltd | 印刷用版下の作製方法 |
-
1978
- 1978-04-18 JP JP4553578A patent/JPS54137397A/ja active Granted
Patent Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS5852640A (ja) * | 1981-09-22 | 1983-03-28 | Toppan Printing Co Ltd | 印刷用版下の作製方法 |
Also Published As
Publication number | Publication date |
---|---|
JPS54137397A (en) | 1979-10-25 |
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