JPS6139617B2 - - Google Patents
Info
- Publication number
- JPS6139617B2 JPS6139617B2 JP52110921A JP11092177A JPS6139617B2 JP S6139617 B2 JPS6139617 B2 JP S6139617B2 JP 52110921 A JP52110921 A JP 52110921A JP 11092177 A JP11092177 A JP 11092177A JP S6139617 B2 JPS6139617 B2 JP S6139617B2
- Authority
- JP
- Japan
- Prior art keywords
- concentration
- electrophoresis
- dye
- gel
- gradient
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
- 239000011544 gradient gel Substances 0.000 claims description 39
- 238000001962 electrophoresis Methods 0.000 claims description 33
- 239000000126 substance Substances 0.000 claims description 28
- 239000000499 gel Substances 0.000 claims description 20
- 239000000178 monomer Substances 0.000 claims description 16
- 238000000465 moulding Methods 0.000 claims description 14
- 239000003431 cross linking reagent Substances 0.000 claims description 12
- 229920000642 polymer Polymers 0.000 claims description 11
- 239000007864 aqueous solution Substances 0.000 claims description 9
- 238000000034 method Methods 0.000 claims description 7
- 239000003505 polymerization initiator Substances 0.000 claims description 7
- 238000004519 manufacturing process Methods 0.000 claims description 5
- 230000000379 polymerizing effect Effects 0.000 claims description 4
- 238000006116 polymerization reaction Methods 0.000 claims description 3
- 230000003247 decreasing effect Effects 0.000 claims description 2
- 238000009826 distribution Methods 0.000 claims description 2
- 239000000243 solution Substances 0.000 description 25
- 102000004169 proteins and genes Human genes 0.000 description 13
- 108090000623 proteins and genes Proteins 0.000 description 13
- HRPVXLWXLXDGHG-UHFFFAOYSA-N Acrylamide Chemical compound NC(=O)C=C HRPVXLWXLXDGHG-UHFFFAOYSA-N 0.000 description 11
- 238000002835 absorbance Methods 0.000 description 10
- 239000007853 buffer solution Substances 0.000 description 8
- 238000013508 migration Methods 0.000 description 7
- 230000005012 migration Effects 0.000 description 7
- 229920002401 polyacrylamide Polymers 0.000 description 6
- 238000002360 preparation method Methods 0.000 description 6
- ROOXNKNUYICQNP-UHFFFAOYSA-N ammonium persulfate Chemical compound [NH4+].[NH4+].[O-]S(=O)(=O)OOS([O-])(=O)=O ROOXNKNUYICQNP-UHFFFAOYSA-N 0.000 description 4
- 102000009027 Albumins Human genes 0.000 description 3
- 108010088751 Albumins Proteins 0.000 description 3
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 3
- LYCAIKOWRPUZTN-UHFFFAOYSA-N Ethylene glycol Chemical compound OCCO LYCAIKOWRPUZTN-UHFFFAOYSA-N 0.000 description 3
- GDTBXPJZTBHREO-UHFFFAOYSA-N bromine Chemical compound BrBr GDTBXPJZTBHREO-UHFFFAOYSA-N 0.000 description 3
- ZIUHHBKFKCYYJD-UHFFFAOYSA-N n,n'-methylenebisacrylamide Chemical compound C=CC(=O)NCNC(=O)C=C ZIUHHBKFKCYYJD-UHFFFAOYSA-N 0.000 description 3
- LHGMHYDJNXEEFG-UHFFFAOYSA-N 4-[4-(dimethylamino)phenyl]iminocyclohexa-2,5-dien-1-one Chemical compound C1=CC(N(C)C)=CC=C1N=C1C=CC(=O)C=C1 LHGMHYDJNXEEFG-UHFFFAOYSA-N 0.000 description 2
- 102000016938 Catalase Human genes 0.000 description 2
- 108010053835 Catalase Proteins 0.000 description 2
- 229910052684 Cerium Inorganic materials 0.000 description 2
- LCGLNKUTAGEVQW-UHFFFAOYSA-N Dimethyl ether Chemical compound COC LCGLNKUTAGEVQW-UHFFFAOYSA-N 0.000 description 2
- 102000008857 Ferritin Human genes 0.000 description 2
- 108050000784 Ferritin Proteins 0.000 description 2
- 238000008416 Ferritin Methods 0.000 description 2
- KWYHDKDOAIKMQN-UHFFFAOYSA-N N,N,N',N'-tetramethylethylenediamine Chemical compound CN(C)CCN(C)C KWYHDKDOAIKMQN-UHFFFAOYSA-N 0.000 description 2
- 229910001870 ammonium persulfate Inorganic materials 0.000 description 2
- KGBXLFKZBHKPEV-UHFFFAOYSA-N boric acid Chemical compound OB(O)O KGBXLFKZBHKPEV-UHFFFAOYSA-N 0.000 description 2
- 239000004327 boric acid Substances 0.000 description 2
- UDSAIICHUKSCKT-UHFFFAOYSA-N bromophenol blue Chemical compound C1=C(Br)C(O)=C(Br)C=C1C1(C=2C=C(Br)C(O)=C(Br)C=2)C2=CC=CC=C2S(=O)(=O)O1 UDSAIICHUKSCKT-UHFFFAOYSA-N 0.000 description 2
- GWXLDORMOJMVQZ-UHFFFAOYSA-N cerium Chemical compound [Ce] GWXLDORMOJMVQZ-UHFFFAOYSA-N 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 235000019441 ethanol Nutrition 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- HFWWEMPLBCKNNM-UHFFFAOYSA-N n-[bis(hydroxyamino)methyl]hydroxylamine Chemical compound ONC(NO)NO HFWWEMPLBCKNNM-UHFFFAOYSA-N 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- 239000004971 Cross linker Substances 0.000 description 1
- ZGTMUACCHSMWAC-UHFFFAOYSA-L EDTA disodium salt (anhydrous) Chemical compound [Na+].[Na+].OC(=O)CN(CC([O-])=O)CCN(CC(O)=O)CC([O-])=O ZGTMUACCHSMWAC-UHFFFAOYSA-L 0.000 description 1
- VGGSQFUCUMXWEO-UHFFFAOYSA-N Ethene Chemical compound C=C VGGSQFUCUMXWEO-UHFFFAOYSA-N 0.000 description 1
- 239000005977 Ethylene Substances 0.000 description 1
- 102000006395 Globulins Human genes 0.000 description 1
- 108010044091 Globulins Proteins 0.000 description 1
- BELBBZDIHDAJOR-UHFFFAOYSA-N Phenolsulfonephthalein Chemical compound C1=CC(O)=CC=C1C1(C=2C=CC(O)=CC=2)C2=CC=CC=C2S(=O)(=O)O1 BELBBZDIHDAJOR-UHFFFAOYSA-N 0.000 description 1
- 101710100170 Unknown protein Proteins 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 238000004364 calculation method Methods 0.000 description 1
- 210000001175 cerebrospinal fluid Anatomy 0.000 description 1
- 238000004440 column chromatography Methods 0.000 description 1
- 230000002596 correlated effect Effects 0.000 description 1
- 230000000875 corresponding effect Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 230000005484 gravity Effects 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- FQPSGWSUVKBHSU-UHFFFAOYSA-N methacrylamide Chemical compound CC(=C)C(N)=O FQPSGWSUVKBHSU-UHFFFAOYSA-N 0.000 description 1
- 239000011259 mixed solution Substances 0.000 description 1
- 239000002808 molecular sieve Substances 0.000 description 1
- UBTYFVJZTZYJHZ-UHFFFAOYSA-N n-[2-(prop-2-enoylamino)propyl]prop-2-enamide Chemical compound C=CC(=O)NC(C)CNC(=O)C=C UBTYFVJZTZYJHZ-UHFFFAOYSA-N 0.000 description 1
- CHDKQNHKDMEASZ-UHFFFAOYSA-N n-prop-2-enoylprop-2-enamide Chemical compound C=CC(=O)NC(=O)C=C CHDKQNHKDMEASZ-UHFFFAOYSA-N 0.000 description 1
- 102000039446 nucleic acids Human genes 0.000 description 1
- 108020004707 nucleic acids Proteins 0.000 description 1
- 150000007523 nucleic acids Chemical class 0.000 description 1
- 230000002572 peristaltic effect Effects 0.000 description 1
- 229960003531 phenolsulfonphthalein Drugs 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 210000002966 serum Anatomy 0.000 description 1
- URGAHOPLAPQHLN-UHFFFAOYSA-N sodium aluminosilicate Chemical compound [Na+].[Al+3].[O-][Si]([O-])=O.[O-][Si]([O-])=O URGAHOPLAPQHLN-UHFFFAOYSA-N 0.000 description 1
- 238000010186 staining Methods 0.000 description 1
- 210000002700 urine Anatomy 0.000 description 1
Landscapes
- Addition Polymer Or Copolymer, Post-Treatments, Or Chemical Modifications (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP11092177A JPS5443881A (en) | 1977-09-14 | 1977-09-14 | Gradient gel for electrophoresis and preparation thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP11092177A JPS5443881A (en) | 1977-09-14 | 1977-09-14 | Gradient gel for electrophoresis and preparation thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS5443881A JPS5443881A (en) | 1979-04-06 |
| JPS6139617B2 true JPS6139617B2 (de) | 1986-09-04 |
Family
ID=14547996
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP11092177A Granted JPS5443881A (en) | 1977-09-14 | 1977-09-14 | Gradient gel for electrophoresis and preparation thereof |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS5443881A (de) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS6095552U (ja) * | 1983-12-07 | 1985-06-29 | サッポロビール株式会社 | ゲル分子量測定装置 |
| JPS62115156U (de) * | 1985-11-18 | 1987-07-22 | ||
| JPH0660885B2 (ja) * | 1987-08-20 | 1994-08-10 | 富士写真フイルム株式会社 | 電気泳動用媒体の製造方法 |
-
1977
- 1977-09-14 JP JP11092177A patent/JPS5443881A/ja active Granted
Also Published As
| Publication number | Publication date |
|---|---|
| JPS5443881A (en) | 1979-04-06 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Choules et al. | An acrylamide gel soluble in scintillation fluid: its application to electrophoresis at neutral and low pH | |
| FI97893C (fi) | Isoelektrinen fokusointimenetelmä ja laite mainitun menetelmän suorittamiseksi | |
| Small | Hydrodynamic chromatography a technique for size analysis of colloidal particles | |
| EP0138763B1 (de) | Verfahren zum Giessen von bei der Dünnschicht-Elektrophorese verwendbaren Gelen | |
| Lewis et al. | The interaction of sodium dodecyl sulfate with methyl cellulose and polyvinyl alcohol | |
| US3384564A (en) | Electrophoretic process for simultaneously spearating and concentrating particles | |
| EP0813062A2 (de) | Einstufige Herstellung von Trennungsmedien für umgekehrte Phasenchromatographie | |
| Righetti et al. | Polymerization kinetics of polyacrylamide gels. III. Effect of catalysts | |
| CN105044234B (zh) | 检测塑料制品中13种甲基丙烯酸/酯特定迁移量的方法 | |
| JPS6139617B2 (de) | ||
| Peschier et al. | Water mobility and structure in poly [2-hydroxyethylmethacrylate] hydrogels by means of the pulsed field gradient NMR technique | |
| Vaerman | [20] Single radial immunodiffusion | |
| Butterman et al. | Ferguson plots based on absolute mobilities in polyacrylamide gel electrophoresis: Dependence of linearity of polymerization conditions and application to the determination of free mobility | |
| VESTERBERG | Quantification of proteins with a new sensitive method—zone immunoelectrophoresis assay | |
| Tulchin et al. | A microgel system for disc electrophoresis | |
| Sola et al. | Tuning capillary surface properties by charged polymeric coatings | |
| Güney | Multiple‐point adsorption of terbium ions by lead ion templated thermosensitive gel: elucidating recognition of conformation in gel by terbium probe | |
| EP2019319B1 (de) | Reagenz für eine Latexaggregationsreaktion und Verfahren zum Nachweis einer Zielsubstanz | |
| Yuk et al. | Contact angles on deformable solids | |
| JP4908590B2 (ja) | キャピラリー電気泳動による分離に続いて行われるテイラー分散の分析による、混合物の成分の流体力学的半径の決定 | |
| JP2597145B2 (ja) | 電気泳動用ゲルの製造方法 | |
| Kaminogawa et al. | Studies on molecular properties of αs1-κ-casein complex by the hydrodynamical methods | |
| JP2794592B2 (ja) | 生体液中の高分子物質の濃縮方法 | |
| Wiener | Polymerization in the system vinylidene chloride‐potassium laurate‐potassium persulfate | |
| JPS5962319A (ja) | 双性イオン分離用電気焦点化装置 |