JPS5443881A - Gradient gel for electrophoresis and preparation thereof - Google Patents

Gradient gel for electrophoresis and preparation thereof

Info

Publication number
JPS5443881A
JPS5443881A JP11092177A JP11092177A JPS5443881A JP S5443881 A JPS5443881 A JP S5443881A JP 11092177 A JP11092177 A JP 11092177A JP 11092177 A JP11092177 A JP 11092177A JP S5443881 A JPS5443881 A JP S5443881A
Authority
JP
Japan
Prior art keywords
concn
soln
coloring matter
gel
vessel
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP11092177A
Other languages
Japanese (ja)
Other versions
JPS6139617B2 (en
Inventor
Tadao Hoshino
Keika Kan
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Mitsubishi Kasei Corp
Original Assignee
Mitsubishi Kasei Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Mitsubishi Kasei Corp filed Critical Mitsubishi Kasei Corp
Priority to JP11092177A priority Critical patent/JPS5443881A/en
Publication of JPS5443881A publication Critical patent/JPS5443881A/en
Publication of JPS6139617B2 publication Critical patent/JPS6139617B2/ja
Granted legal-status Critical Current

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  • Investigating Or Analysing Biological Materials (AREA)
  • Addition Polymer Or Copolymer, Post-Treatments, Or Chemical Modifications (AREA)

Abstract

PURPOSE: To prepare the gel described in the titled which requires no addn. of conventional material and which makes easy detection of gel concn. at a specific position, by addn. of a coloring matter to one of two aq. soln. contg. a monomer, a crosslinking agent, and a polymerization initiator, and by making coloring matter concn. in a gel correlate with produced crosslinked polymer concn.
CONSTITUTION: An aq. soln. 1 of higher concn. made by dissolving a polymer, a crosslinking agent, a polymerization initiator, and a polymerization promotor in a suitable buffer soln. is fed to a vessel 2, and an aq. soln. 3 of lower concn. consisting of the above-mentioned materials is fed to a vessel. A stirrer 5 is started to rotate, then a pump 9 is operated and simultaneously a valve 6 is opened. When the lower concn. soln. 3 is moved to a molding instrument 11, the higher concn. soln. 1 moves to the vessel 4 by gravity. If the liq. levels in the vessels 2, 4 are maintained equal, coloring matter concn. of the low concn. soln. 3 increases with increase in the concn. of the soln. 3. Consequently, measurement of the coloring matter concn. of a gradient gel contained in the molding instrument 11 can show gel concn. When the coloring matter concn. is correlated with migrated position of a specific material, the mol.wt. of an unknown material is given from the migrated position, and the unknown material is identified.
COPYRIGHT: (C)1979,JPO&Japio
JP11092177A 1977-09-14 1977-09-14 Gradient gel for electrophoresis and preparation thereof Granted JPS5443881A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP11092177A JPS5443881A (en) 1977-09-14 1977-09-14 Gradient gel for electrophoresis and preparation thereof

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP11092177A JPS5443881A (en) 1977-09-14 1977-09-14 Gradient gel for electrophoresis and preparation thereof

Publications (2)

Publication Number Publication Date
JPS5443881A true JPS5443881A (en) 1979-04-06
JPS6139617B2 JPS6139617B2 (en) 1986-09-04

Family

ID=14547996

Family Applications (1)

Application Number Title Priority Date Filing Date
JP11092177A Granted JPS5443881A (en) 1977-09-14 1977-09-14 Gradient gel for electrophoresis and preparation thereof

Country Status (1)

Country Link
JP (1) JPS5443881A (en)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS6095552U (en) * 1983-12-07 1985-06-29 サッポロビール株式会社 Gel molecular weight measuring device
JPS62115156U (en) * 1985-11-18 1987-07-22
JPS6449951A (en) * 1987-08-20 1989-02-27 Fuji Photo Film Co Ltd Production of medium for electrophoresis

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS6095552U (en) * 1983-12-07 1985-06-29 サッポロビール株式会社 Gel molecular weight measuring device
JPS62115156U (en) * 1985-11-18 1987-07-22
JPS6449951A (en) * 1987-08-20 1989-02-27 Fuji Photo Film Co Ltd Production of medium for electrophoresis

Also Published As

Publication number Publication date
JPS6139617B2 (en) 1986-09-04

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