JPS5443881A - Gradient gel for electrophoresis and preparation thereof - Google Patents
Gradient gel for electrophoresis and preparation thereofInfo
- Publication number
- JPS5443881A JPS5443881A JP11092177A JP11092177A JPS5443881A JP S5443881 A JPS5443881 A JP S5443881A JP 11092177 A JP11092177 A JP 11092177A JP 11092177 A JP11092177 A JP 11092177A JP S5443881 A JPS5443881 A JP S5443881A
- Authority
- JP
- Japan
- Prior art keywords
- concn
- soln
- coloring matter
- gel
- vessel
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- Investigating Or Analysing Biological Materials (AREA)
- Addition Polymer Or Copolymer, Post-Treatments, Or Chemical Modifications (AREA)
Abstract
PURPOSE: To prepare the gel described in the titled which requires no addn. of conventional material and which makes easy detection of gel concn. at a specific position, by addn. of a coloring matter to one of two aq. soln. contg. a monomer, a crosslinking agent, and a polymerization initiator, and by making coloring matter concn. in a gel correlate with produced crosslinked polymer concn.
CONSTITUTION: An aq. soln. 1 of higher concn. made by dissolving a polymer, a crosslinking agent, a polymerization initiator, and a polymerization promotor in a suitable buffer soln. is fed to a vessel 2, and an aq. soln. 3 of lower concn. consisting of the above-mentioned materials is fed to a vessel. A stirrer 5 is started to rotate, then a pump 9 is operated and simultaneously a valve 6 is opened. When the lower concn. soln. 3 is moved to a molding instrument 11, the higher concn. soln. 1 moves to the vessel 4 by gravity. If the liq. levels in the vessels 2, 4 are maintained equal, coloring matter concn. of the low concn. soln. 3 increases with increase in the concn. of the soln. 3. Consequently, measurement of the coloring matter concn. of a gradient gel contained in the molding instrument 11 can show gel concn. When the coloring matter concn. is correlated with migrated position of a specific material, the mol.wt. of an unknown material is given from the migrated position, and the unknown material is identified.
COPYRIGHT: (C)1979,JPO&Japio
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP11092177A JPS5443881A (en) | 1977-09-14 | 1977-09-14 | Gradient gel for electrophoresis and preparation thereof |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP11092177A JPS5443881A (en) | 1977-09-14 | 1977-09-14 | Gradient gel for electrophoresis and preparation thereof |
Publications (2)
Publication Number | Publication Date |
---|---|
JPS5443881A true JPS5443881A (en) | 1979-04-06 |
JPS6139617B2 JPS6139617B2 (en) | 1986-09-04 |
Family
ID=14547996
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP11092177A Granted JPS5443881A (en) | 1977-09-14 | 1977-09-14 | Gradient gel for electrophoresis and preparation thereof |
Country Status (1)
Country | Link |
---|---|
JP (1) | JPS5443881A (en) |
Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS6095552U (en) * | 1983-12-07 | 1985-06-29 | サッポロビール株式会社 | Gel molecular weight measuring device |
JPS62115156U (en) * | 1985-11-18 | 1987-07-22 | ||
JPS6449951A (en) * | 1987-08-20 | 1989-02-27 | Fuji Photo Film Co Ltd | Production of medium for electrophoresis |
-
1977
- 1977-09-14 JP JP11092177A patent/JPS5443881A/en active Granted
Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS6095552U (en) * | 1983-12-07 | 1985-06-29 | サッポロビール株式会社 | Gel molecular weight measuring device |
JPS62115156U (en) * | 1985-11-18 | 1987-07-22 | ||
JPS6449951A (en) * | 1987-08-20 | 1989-02-27 | Fuji Photo Film Co Ltd | Production of medium for electrophoresis |
Also Published As
Publication number | Publication date |
---|---|
JPS6139617B2 (en) | 1986-09-04 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
DK148392C (en) | PROCEDURE FOR POLYMERIZATION OR COPOLYMERIZATION OF ONE OR MORE 1-OLEPHINE Monomers | |
DK144274C (en) | PROCEDURE FOR POLYMERIZATION OR COPOLYMERIZATION OF ALFA OLEFINES AND SOLID CATALYTIC COMPLEX FOR USE BY THE PROCEDURE | |
JPS55110105A (en) | Preparation of polymer composition containing physiologically active material | |
JPS5443881A (en) | Gradient gel for electrophoresis and preparation thereof | |
JPS5679113A (en) | Low-shrinkage unsaturated polyester resin composition | |
JPS5233986A (en) | Preparation of ethylene homo-or copolymer | |
DK127511B (en) | Process for the polymerization or copolymerization of vinyl chloride under reduced pressure. | |
JPS5343742A (en) | Tackifying substance and its preparation | |
JPS5430281A (en) | Improved preparation of acrylonitrile-type polymer melt | |
JPS5310683A (en) | New oligomer, its preparation and emulsion polymerization using the same | |
DK128499B (en) | Process for the continuous polymerization or copolymerization of vinyl chloride in aqueous suspension or in aqueous fine suspension using an inoculum. | |
JPS5421484A (en) | Polymerization of vinyl chloride | |
JPS5329386A (en) | Preparation of heat-stable vinyl chloride polymer | |
JPS5336586A (en) | Production of vinyl chloride or cinylidene chloride polymer | |
IE44309L (en) | Polymerization | |
Voort | Etching techniques for image analysis | |
JPS5341386A (en) | Preparation of acrylamide polymer | |
JPS55164348A (en) | Comparison electrode consisting of field effect transistor | |
JPS5485292A (en) | Preparation of liquid chloroprene polymer which can be loaded with large amount of fillers | |
Zurakowska-Orszagh et al. | Initiation of Solid-Phase Polyreaction. I. Chemical Initiation | |
JPS5252984A (en) | Proces for polymerizing a vinyl monomer | |
JPS5686911A (en) | Preparation of polyvinyl chloride having improved thermal stability | |
JPS53129288A (en) | Preparation of polymer emulsion | |
AT338519B (en) | PROCESS FOR THE POLYMERIZATION AND COPOLYMERIZATION OF VINYL CHLORIDE | |
JPS55155004A (en) | Methyl methacrylate syrup composition excellent in storage stability |