JPS6136296A - 生体膜蛋白質の分離精製法 - Google Patents

生体膜蛋白質の分離精製法

Info

Publication number
JPS6136296A
JPS6136296A JP15764384A JP15764384A JPS6136296A JP S6136296 A JPS6136296 A JP S6136296A JP 15764384 A JP15764384 A JP 15764384A JP 15764384 A JP15764384 A JP 15764384A JP S6136296 A JPS6136296 A JP S6136296A
Authority
JP
Japan
Prior art keywords
surfactant
membrane proteins
separation
gel electrophoresis
weight
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP15764384A
Other languages
English (en)
Japanese (ja)
Other versions
JPH0521118B2 (OSRAM
Inventor
Toshio Takagi
俊夫 高木
Masahiro Fukuda
正博 福田
Kazuo Obe
大部　一夫
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Lion Corp
Original Assignee
Lion Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Lion Corp filed Critical Lion Corp
Priority to JP15764384A priority Critical patent/JPS6136296A/ja
Priority to US06/758,247 priority patent/US4654132A/en
Priority to DE19853527139 priority patent/DE3527139A1/de
Publication of JPS6136296A publication Critical patent/JPS6136296A/ja
Publication of JPH0521118B2 publication Critical patent/JPH0521118B2/ja
Granted legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/24—Extraction; Separation; Purification by electrochemical means
    • C07K1/26—Electrophoresis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44704—Details; Accessories
    • G01N27/44747—Composition of gel or of carrier mixture

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Electrochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Dispersion Chemistry (AREA)
  • Biophysics (AREA)
  • Biomedical Technology (AREA)
  • Physics & Mathematics (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
JP15764384A 1984-07-30 1984-07-30 生体膜蛋白質の分離精製法 Granted JPS6136296A (ja)

Priority Applications (3)

Application Number Priority Date Filing Date Title
JP15764384A JPS6136296A (ja) 1984-07-30 1984-07-30 生体膜蛋白質の分離精製法
US06/758,247 US4654132A (en) 1984-07-30 1985-07-24 Separation and purification of biomembrane proteins
DE19853527139 DE3527139A1 (de) 1984-07-30 1985-07-29 Abtrennung und reinigung von biomembranproteinen

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP15764384A JPS6136296A (ja) 1984-07-30 1984-07-30 生体膜蛋白質の分離精製法

Publications (2)

Publication Number Publication Date
JPS6136296A true JPS6136296A (ja) 1986-02-20
JPH0521118B2 JPH0521118B2 (OSRAM) 1993-03-23

Family

ID=15654205

Family Applications (1)

Application Number Title Priority Date Filing Date
JP15764384A Granted JPS6136296A (ja) 1984-07-30 1984-07-30 生体膜蛋白質の分離精製法

Country Status (3)

Country Link
US (1) US4654132A (OSRAM)
JP (1) JPS6136296A (OSRAM)
DE (1) DE3527139A1 (OSRAM)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2011501748A (ja) * 2007-10-11 2011-01-13 プロメガ コーポレイション 切断可能な界面活性剤
JP2021510811A (ja) * 2018-01-12 2021-04-30 アジレント・テクノロジーズ・インクＡｇｉｌｅｎｔ Ｔｅｃｈｎｏｌｏｇｉｅｓ， Ｉｎｃ． 電気泳動分離用分解剤としてのトリ−およびテトラ−ヒドロキシル第四級アンモニウム化合物の使用

Families Citing this family (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5084150A (en) * 1987-03-24 1992-01-28 Northeastern University Selective high performance electrokinetic separations employing the surface of moving charged colloidal particles
US5283196A (en) * 1988-01-12 1994-02-01 The United States Of America As Represented By The Department Of Health And Human Services Polyacrylamide gels with improved detection of protein
US5219923A (en) * 1988-04-29 1993-06-15 At Biochem, Inc. Electrophoretic media
US5055517A (en) * 1988-04-29 1991-10-08 At Biochem Electrophoretic media
US5292665A (en) * 1989-03-15 1994-03-08 The United States Of America As Represented By The Department Of Health And Human Services Catalyst for preparing polyacrylamide gel which improves the detection of biomaterials by silver staining
US5957203A (en) * 1992-08-31 1999-09-28 Union Oil Company Of California Ultra-high temperature stable gels
US5617920A (en) * 1992-08-31 1997-04-08 Union Oil Company Of California Method for modifying gelation time of organically crosslinked, aqueous gels
US5246073A (en) * 1992-08-31 1993-09-21 Union Oil Company Of California High temperature stable gels
AU5171293A (en) * 1992-10-14 1994-05-09 Regents Of The University Of Colorado, The Ion-pairing of drugs for improved efficacy and delivery
US5726154A (en) * 1996-06-28 1998-03-10 University Of Utah Research Foundation Stabilization and oral delivery of calcitonin
US6758953B2 (en) 1999-10-28 2004-07-06 Nathan A. Thomas Multistage electrophoresis apparatus and method of use for the separation and purification of cells, particles and solutes
DE10027705A1 (de) * 2000-06-03 2001-12-13 Erich Sackmann Verfahren zur elektrophoretischen Auftrennung von Membranproteinen
US20050148100A1 (en) * 2003-12-30 2005-07-07 Intel Corporation Methods and devices for using Raman-active probe constructs to assay biological samples
FR2869995B1 (fr) * 2004-05-10 2006-09-22 Sebia Sa Procede ameliore de separation de proteines par electrophorese capillaire et compositions de tampon pour electrophorese capillaire
US7731829B2 (en) * 2006-02-13 2010-06-08 Expedion, Inc. Electrophoresis gel and method of making same
US8282800B2 (en) 2008-09-02 2012-10-09 Bio-Rad Laboratories, Inc. Hydrolysis-resistant polyacrylamide gels
US20110065643A1 (en) 2009-06-12 2011-03-17 University Of Southern California Clusterin Pharmaceuticals and Treatment Methods Using the Same
WO2014150275A1 (en) 2013-03-15 2014-09-25 Bio-Rad Laboratories, Inc. Polyacrylamide gels for rapid casting, blotting, and imaging, with storage stability
WO2021030427A2 (en) * 2019-08-12 2021-02-18 Yale University Intragel well sample loading system
WO2024056508A1 (en) 2022-09-15 2024-03-21 Cube Biotech Gmbh In vitro diagnostic method for detecting the presence of a target by using stabilized membrane proteins

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4481094A (en) * 1982-05-17 1984-11-06 Techamerica Group, Inc. Stabilized polyacrylamide gels and system for SDS electrophoresis
JPS6060548A (ja) * 1983-09-14 1985-04-08 Fuji Photo Film Co Ltd 電気泳動用媒体

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2011501748A (ja) * 2007-10-11 2011-01-13 プロメガ コーポレイション 切断可能な界面活性剤
JP2021510811A (ja) * 2018-01-12 2021-04-30 アジレント・テクノロジーズ・インクＡｇｉｌｅｎｔ Ｔｅｃｈｎｏｌｏｇｉｅｓ， Ｉｎｃ． 電気泳動分離用分解剤としてのトリ−およびテトラ−ヒドロキシル第四級アンモニウム化合物の使用
US11815488B2 (en) 2018-01-12 2023-11-14 Agilent Technologies, Inc. Use of tri- and tetra-hydroxyl quaternary ammonium compounds as resolving agents for electrophoretic separations

Also Published As

Publication number Publication date
US4654132A (en) 1987-03-31
DE3527139A1 (de) 1986-02-06
JPH0521118B2 (OSRAM) 1993-03-23

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