JPS612865A - Sampling method of high density blood component - Google Patents

Abstract

(57) [Summary] This bulletin contains application data before electronic filing, so abstract data is not recorded.

Description

DETAILED DESCRIPTION OF THE INVENTION The present invention relates to a method for collecting blood components containing a high density of components to be collected, such as granulocytes and platelets, from blood of a blood donor.

FIG. 1 shows a conventional blood component collection method. In FIG. 1, blood is collected from a blood donor 32 using a roller pump 8, and the rotation speed of the roller pump 7 is set so that the blood anticoagulant 28 is at a constant ratio. Then, add an appropriate amount of physiological saline 29 and temporarily store it in the blood bag. Next, the valve 14 is closed, and the roller pump 9 is turned to introduce an appropriate amount of blood into the centrifuge container from the blood inlet 4 through the opening. At the same time, the valve 1 is closed, the valve 2 is opened, and the roller pump 2 is rotated to collect the sterilized air in the centrifuge container 20 through the opening 23 and the blood inlet 3 into the blood bag %. After putting an appropriate amount of blood into the centrifuge container 20, the roller pumps 9 and n are stopped. The blood in the centrifugation container forms a separation layer of red blood cells 17, white blood cells, and blood plasma D in this order from the outer periphery due to the centrifugal force proportional to the square of the number of rotations and the radius of rotation. By making the upper surface of the centrifugal separation vessel transparent, the boundary between the separation layers can be detected.

By pumping whole blood with fine adjustment using the pump 9, the separation layer within the centrifugal separation container m is moved toward the center.

When a portion of the red blood cells 17, most of the white blood cells, and a portion of the hemoglobin reach the concave portion, it is visually or automatically detected and the rotation is stopped, and components including granulocytes accumulate in the concave portion.

Red blood cells 17 and whole blood accumulate in the four parts, and blood flows into the recesses 5
19 accumulates. Open the valve, and turn the pump t to pump the components into the blood bag 26 through the opening 1 and the blood inlet 1.
Store in. The component of the recess 35 is removed by turning the pump 2 to the opening η
The blood passes through the blood outlet 2 and is stored in the blood bag 27. The components in the fourth part 36 are collected in the blood bag 26 through the blood outlet 3 through the opening device by turning the pump (3). When there are no more components in the centrifugal separation container 20, the pumps p1 and p1 are stopped. A suitable amount of physiological saline is added to the components of the blood bag 26, passed through a felter 31, and the roller pump B is turned to return the blood to the blood donor 32. By performing the above operations multiple times, a large amount of platelets and granulocytes accumulate in the blood bag 27.

Next, the components of the blood bag 27 are returned to the centrifugation container by turning the roller pump 2, and centrifugation is performed. Once a wide separation layer of white blood cells is formed, whole blood from the blood bank δ is sent in, and the component layer to be collected is moved to the recess. Once the transfer is complete, stop the rotation of the centrifuge container. The components in the recess 36 are collected in the blood bag 27 by closing the valve, opening the valve I, and rotating the roller pump U. The components of the fourth part are collected in the blood bag 26 by turning the roller pump υ, and then returned to the blood donor by the roller pump B. By the above operations, a component containing granulocytes or platelets at a high density is obtained from the blood bag n.

However, this conventional method has the following drawbacks.

1) Since the components accumulated in the recess are put into the blood bag 27 by the roller pump ν, and conversely, the components are returned to the fourth part 35, a large amount of blood cells die when passing through the pump 2.

The structure of the centrifugation container is complicated because there are four openings communicating with the recesses of the centrifugation container and four blood inflow ports for the openings.

The total number of pumps is large because two roller pumps are used: the roller pump 9 that pumps whole blood into the recess and the roller pump 10 that sucks out red blood cells from the four parts.

4) When sucking out the ingredients from the recesses 34, 35, 36,
Since air is sent into the centrifuge container area from the blood puncture 26 through the valve shell and the opening U of the blood inflow port 11, the air and red blood cells mix at the opening and form bubbles, which is not desirable as a component for returning blood. .

5) The component is sucked out from the fourth part 36 through the opening, but since there is only one opening and the area of the opening 36 is small compared to the bottom area of the recess, the component remaining on the bottom surface separated from the opening is small. The quantity is large.

An object of the present invention is to eliminate the drawbacks of the above-mentioned conventional techniques and to collect platelets, granulocytes, or both components of platelets and granulocytes, which are contained in trace amounts in blood, at a high density. When collecting both platelet and granulocyte components in a high M column,
This will be explained as a representative example, but the same applies when collecting granulocytes at high density and when collecting platelets at high density.

The present invention performs separation multiple times, increases the density of the components to be collected in the centrifugation container each time the separation is repeated, and when an appropriate density is reached, part of the separation is completed and the components are transferred to blood. It is collected in a bag.

Next, the present invention will be explained in detail with reference to FIGS. 2 and 3.

Blood is collected from the donor 32 using the roller pump 8, the rotation speed of the roller bong is set so that the blood anticoagulant column is at a constant ratio, an appropriate amount of physiological saline is added, and the blood is pumped once.
Store in. Next, close the valves E and 16, open the valve 14, turn the roller pump 9, and pour an appropriate amount of blood into the centrifuge container 20 from the blood inlet l through the opening 21. Then, close the valve M and turn the roller pump 9. and start the centrifuge timer. Although the centrifugation timer is not shown in the diagram,
It will stop after the set time has elapsed. The blood in the centrifugation container forms separated layers of red blood cells 17, white blood cells 18, and plasma 19 in this order from the outer periphery due to the centrifugal force proportional to the square of the number of rotations and the radius of rotation. By making the upper surface of the centrifugation container 20 transparent, the boundary between the separation layers can be automatically detected by a sensor field.

When the centrifugation timer stops and the leukocyte lull is at the sensor detection position, the rotation of the centrifuge container hole is stopped. If white blood cells are not at the detection position of the sensor 33, put whole blood into the centrifuge container using the roller pump 9, or close the valve 14, open the valve, and rotate the centrifuge container 20 at a low rotation speed. The red blood cells 17 are fed through the opening 21 through the blood inlet 1 into the blood bag 26 by rotating the roller bomb 9 to move the separation layer in the centrifuge container 20, and the white blood cells come to the detection position of the sensor 33. When the sensor 33 detects this, the rotation of the centrifugal separation container hole and the roller pump 9 is stopped. Red blood cells 17 are collected in the fourth part 34, part of the red blood cells 17 containing many granulocytes and part of plasma 19 containing many white blood cells and platelets are collected in the recessed part 36, and plasma D is collected in the recessed part 36. Next, close the valve 14, open the valve 15, rotate the roller pump 9 to remove the component in the recess, and open the opening 2.
1, the blood flows through the blood inlet 1 and accumulates in the blood bag 26. The component in the recess is collected in the blood bag through the blood outflow port 2 from the opening n by rotating the roller pump (blood bag 2).
Ingredients 6 add an appropriate amount of physiological saline, filter 1
The blood is returned to the blood donor 32 by rotating the roller pump B. By repeating the above action multiple times, the density of the granulocytes and blood plasma plate in the recess increases in proportion to the number of times the action is performed.

Next, the components accumulated in the recess 35 are directly transfused into the patient from the transparent rotating container via the blood inlet 1. When collecting granulocytes or platelets at a high density, the only difference is the detection position of the sensor 33, and other operations remain the same.

According to the present invention, it is easy and reliable to collect a blood component containing a high density of granulocytes, a blood component containing a high density of platelets, and a blood component containing a high density of granulocytes and platelets from the whole blood of a blood donor. , the collection time is short. This means that when a large amount of blood is taken from a donor, only the blood components that are contained in minute amounts are taken out, and the remaining blood is returned to the donor, it can be collected with high efficiency, and the short collection time means that the donor It is also very effective in terms of reducing the burden. In addition, since blood is transfused directly to the patient from the transparent rotating container, there is no need for a blood transfusion bag.

[Brief explanation of the drawing]

FIG. 1 is an explanatory diagram showing a conventional blood component collection method, and FIGS. 2 and 3 are explanatory diagrams showing a blood component collection method according to the present invention. l is blood inlet, 2 is blood outlet, 3 is blood outlet, 4
is blood outflow port, 5 is sole, 6 is seal, 7 is roller pump, 8 is roller pump, 9 is roller pump, t is roller pump, ■ is roller pump, 12 is roller pump, B
is a roller pump; 14 is a valve; U is an opening, 25 is a blood bag, 26 is a blood bag, 27 is a blood bag, 28 is a blood anticoagulant, 29 is a physiological saline, 30
is saline, 31 is filter, 32 is blood donor, 33
is the sensor, and a is the air inlet. , t + 42 figures le 3 figures

Claims (1)

[Claims] In a concentric circle centered on the rotation axis, radially a from the center
In a centrifugal separator equipped with a rotating container having three concave chambers consisting of a chamber, a chamber B, and a chamber C, 1) Blood collected from a donor is injected into chamber C of the rotating container, and blood is sequentially injected from chamber B to chamber A. 2) After rotating the rotating container and centrifuging for a set time, move the separation layer until the layer of the desired blood component is located in chamber B, 3) Stop the rotation, and fill chamber A of the rotating container. High-density blood from which at least one of granulocytes or platelets is collected at a high density, characterized by returning the blood components accumulated in chamber c to the blood donor and repeating steps 1) to 3) a predetermined number of times. How to collect ingredients.