JPS58139754A - Rotor for centrifugal separation - Google Patents

Abstract

PURPOSE:To separate blood constituent etc. that contains white corpuscles at high density in a short time and reduce the size and weight of a roller pump by providing concentric projections around the rotation axis of a blood separating rotor and connecting the concave chamber of inner and outer diameter side and the concave chamber provided at the projection to outside. CONSTITUTION:Anticoagulant 27 is added to blood collected continously from a blood donor 15 by a roller pump 23, diluted with physiological saline solution 28, stored in a bag 21, and a quantity of blood corresponding to inner volume of a rotor 3' is sent in by a roller pump 19. The blood is led to the chamber of the rotor 3' rotating at high speed through a blood inlet 1 and an opening 12, and separated layers of red corpuscles 9, white corpuscles 10, blood plasma 11 are formed in a short time from outer periphery side. When the layer of white corpuscles 10 is outer side from the concave chamber of the projection 18, very small quantity of blood is sent in while adjusting by the pump 19 and moved to the concave chamber. When it becomes as shown in the figure, the pump 19 and rotor 3' are stopped. At this time each constituent collects in each concave chamber, and when roller pumps 17, 25, 26 are rotated, white corpuscles 10 shift to the bag 8, and red corpuscles and blood plasma shift to a bag 22. Small pumps may be satisfactorily used for this purpose as they are only required to rotate after the rotor is stopped.

Description

DETAILED DESCRIPTION OF THE INVENTION The present invention relates to a centrifugal rotor that facilitates the collection of blood components containing granulocytes, platelets, etc. under high illumination from the blood of a blood donor.

FIG. 1 shows a conventional centrifugal rotor.

In FIG. 1, blood collected from a blood donor 15 by a roller pump p is introduced into a chamber of a centrifugal rotor 3 through an opening [fd] through a blood inlet 1. A stationary seal 4 that is integral with the blood inlet 1 and the blood outlet 2 is connected to the centrifuge rotor 3.
It has a structure that maintains airtightness together with the rotary seal 5 which is integrated with the rotary seal 5.

Inside the rotating rotor 3, red blood cells 9, white blood cells 10. Separation layers are formed in the order of plasma 11. When the white blood cells lO) are located above the opening 13, the roller honda 19 is stopped, the roller pump 7 is rotated to suck out the white blood cells lυ from the opening 13, and when the white blood cells lυ are collected in the blood bag 8, the pump 7 is stopped. let The blood remaining in the chamber of the rotor 3 is returned to the donor 15 by the roller pump 6.
The above procedure is repeated multiple times to collect a large number of white blood cells 10 into a blood bag 8, which is then used for blood transfusion to a patient.

However, according to this prior art, the white blood cells 10 are transferred to the opening 13.
Since a pumping action that overcomes the centrifugal force acting on the blood is required when collecting blood into the blood bag 8, the roller pump 7 must be strong. It is possible to reduce the centrifugal force so that a normal roller pump can be used, but in order to do so, the radius of the rotor 3 must be made smaller.
I have to lower the rpm. It may be possible to reduce the total radius and deepen the depth of the rotor chamber to make the processing capacity the same as before and prevent a decrease in the processing speed of blood components, but this would reduce the width of the separation In of leukocytes. The area becomes narrower, making it difficult to collect trace components such as condylar globules.
When the rotational speed is lowered, the centrifugal force decreases in proportion to the square of the rotational speed, and the centrifugation time increases, resulting in a reduction in processing speed.

An object of the present invention is to eliminate the above-mentioned drawbacks of the prior art and to easily and quickly collect a blood component containing a high density of white blood cells, X7, which is contained in only a trace amount in blood. The explanation will mainly be made using white blood cells as an example, but the same will apply to the collection of actually necessary components containing a high density of granulocytes or components containing a high density of platelets. Do not centrifuge with
For this purpose, a separation layer is created by high-speed rotation, and the whole blood is fed while controlling the outer circumference of the rotor chamber, that is, the entire surface in the direction of centrifugal force.When the leukocyte loss to be collected reaches the concave chamber above the opening, The structure is such that when the rotation of the roller pump 19 and the rotor 3 is stopped, the red blood cells 9, blood cells, and plasma 11 can be collected in concave chambers corresponding to their respective positions. Blood components 9, ILI, 11 are from the opening ν, 16.17,
By turning the respective roller pumps 26, 25, and 17 f at the same time, the sample can be collected all at once.

Next, FIG. 2 to FIG. 4 1f (more detailed explanation of the present invention).

Blood anticoagulation 412 is applied to blood continuously collected from blood donor 15.
7i roller pump 23 to add at an appropriate ratio.
The blood is suitably diluted with physiological saline 28 and then stored in a blood bag 21, and a roller pump 19 sends a sufficient amount of blood into the internal volume of the rotor. The blood enters the chamber of the rotating centrifugal rotor 3' through the opening and closing of the blood inlet 11, and forms a separation layer of red blood cells 9, white blood cells, and plasma 11 in this order from the outer circumferential side. The layer of white blood cells 10 is located at the outer periphery 1111j from the concave chamber of the convex part.
When Vct is lower than Vct, the roller pump 19 is used to adjust the amount of gj and move it to the concave chamber, and when the state shown in FIG. 4 is reached, the roller pump p and the centrifugal rotor 3 are stopped. FIG. 3 shows the state in which each component accumulates in each concave chamber at that time. Next, when the roller pumps 17, 25, and 26 are turned at the same time, the white blood cells flow into the blood bag δ, and the red blood cells 9 and plasma 11 are transferred to the blood bag 22. The air bubbles sucked in together escape to the upper VC of the blood bag 22, and the blood to be returned sinks to the lower part. If the blood bag 22 is placed at a sufficiently high place, the blood will be returned to the donor 15 through a filter due to the drop. At this time, physiological saline 29 is added as necessary. A blood return pump may also be used (not shown).

By repeating the above operations multiple times, the desired number of white blood cells can be obtained.

Next, what is actually needed is often granulocytes and platelets.

It detects from the transparent outer wall of the centrifugal rotor 3' that the boundary between plasma 11 and white blood cells comes to the convex plane 31 on the outer peripheral side of the convex part, and then finely adjusts the amount of whole blood to be fed to make the boundary concave. If the roller pump 19 and centrifugal rotor 3' are stopped when the room becomes busy, blood containing a high density of blood clots can be collected into the blood bag 8. This is probably because platelets are abundant in the plasma of the buffy coat, and there is a boundary between plasma 11 and white blood cells on the inner convex plane 32 of the convex portion! Observing this through the transparent outer wall, a small amount of the treasure was pumped using the roller pump D, and 11 white blood cells were placed on the concave chamber.
) and the red blood cells 9, by stopping the roller pump p and the rotor 3', blood components containing a high density of granulocytes can be collected into the blood clog 8. This is because it is contained in large quantities.

The space between the inner convex element dEI32 and the convex plane 3N of the outer circumferential surface 3N is between the transparent outer wall of the rotor 3'! r - If you make it narrow,
Since the separation layer passing through it is magnified, white blood cells closer to plasma (containing a high density of platelets) and white blood cells closer to red blood cells (containing a high density of granulocytes) can be easily detected.

Since the high-speed rotation generates high centrifugal force for separation, each centrifugation time can be shortened. When collecting blood components into a blood pump, the centrifugal rotor is stopped before the roller pump is turned on, so a small, lightweight, and inexpensive pump can be used. Since blood components for return and blood components to be collected for transfusion to a patient can be collected simultaneously, the collection time can be shortened.

Not only can white blood cells be collected at a high rate, but blood components containing a high concentration of platelets and blood components containing a high density of granulocytes can also be collected.

[Brief explanation of the drawing]

FIG. 1 is a longitudinal sectional view of a conventional centrifugal rotor. Second
4 to 4 are longitudinal sectional views of the centrifugal rotor of the present invention. l is blood inlet, 2 is blood outlet, 3.3' is centrifuge rotor, 4 is stationary seal, 5 is rotating cool, 6Fi roller pump, 7 is roller pump, 8 is blood bag, 9 is red blood cell, powder is white blood cell, 11 is plasma, 12 is opening, 13 is opening, 14 is reservoir, b is blood donor, plaque is opening, 17 is opening, partner is convex part, p is roller pump, 20 is blood Outlet, 21 is a blood bag, 22 is a blood bag, 23 is a roller pump, 24 roller lab Patent applicant's name Hitachi Koki Co., Ltd. -: I2 [? ]

Claims (1)

[Claims] There is a concentric convex part around the rotation axis, and in addition to a concave chamber formed on the outer diameter of this convex part and a concave chamber formed on the inner diameter side, a concentric concave chamber is also provided on the upper surface of the convex part, and each concave chamber and the outside A centrifugal rotor characterized by having an independent opening that connects.