JPS58138464A - Sampling of high density blood component - Google Patents

Abstract

(57) [Summary] This bulletin contains application data before electronic filing, so abstract data is not recorded.

Description

DETAILED DESCRIPTION OF THE INVENTION The present invention relates to a method for collecting blood components containing a high density of components to be collected, such as granulocytes and platelets, from blood of a blood donor. - Figure 1 shows a conventional blood component collection method. In FIG. 1, blood collected from a donor by a roller pump 19 is introduced into a centrifugal rotor 30 chamber from a blood inlet 1 through an opening 12. A stationary seal 4 that is integral with the blood inlet 1 and an outlet 2 is a rotating seal 5 that is integral with the centrifugal rotor 3.
It also has a structure that maintains airtightness.

Inside the rotating centrifugal rotor 3, red blood cells 9 are collected from outside the outer periphery.
, a separate layer is formed between the white blood cells 101 and 11 of the plasma 11.

- When a layer of white blood cells 10 is located above the mouth 13, stop the roller pump 19, rotate the roller pump 7 to suck out the white blood cells 10 from the opening 13, collect it in the blood bag 8, and then turn off the pump 7. make it stop. Blood remaining in the chamber of the rotor 3 is returned to the blood donor 15 by the roller pump 6. The above procedure is repeated several times to collect a large number of white blood cells into the blood bag 8.

However, this conventional method has the following drawbacks. it is,
White blood cells io (This area is called the puffy coat, which is mainly composed of white blood cells) Fi is extremely thin and the separation layer is narrow, so it is difficult to extract this much, and it also contains a large amount of red blood cells 9 and plasma 11. The amount of τ sampled is only 7. Therefore, the leukocyte retention area of the collected component is extremely low.

Moreover, the granulocytes, which are part of the buffy coat, are removed from the local side.
(It is difficult to collect platelets or collect platelets at a high density.

The object of the present invention is to eliminate the drawbacks of the above-mentioned and conventional techniques and to collect leukocyte-containing components at a high density, which are contained in minute amounts in lambda blood. The explanation will be given using white blood cells as a typical example, but the same applies when collecting actually necessary components containing a high density of granulocytes or components containing a high density of platelets.

In the present invention, after first performing a plurality of rough separations, the crude separators from the plurality of times are re-separated one or more times to obtain a purified product. The explanation will be limited to white blood cells as what is to be collected. The separation layer formed inside the centrifugal rotor consists of red blood cells, white blood cells, and plasma in this order from the outer circumferential side, and if the amount of each is RlwXpz, then the amount of W is k I'm I
I] is very narrow but contains some red blood cells (△R) and plasma (A
If Pf ) can also be extracted, it can be easily collected.

If you repeat this process once, will it produce a crude separated product? L(ΔR
+W +t2j-) is obtained and stored in an external container.

Another part of this crude separation is returned to the rotor chamber to form a separation layer, and as shown in Fig. 2, red blood cells (-, ΔR) h1 white blood cells (n, W) 17, plasma (?L,, Bei) 18 In particular, since the layer of white blood cells 17 is n times as large as that in whole blood separation, collection of only white blood cells becomes easy. Assuming that P1 is simultaneously extracted into a small amount of red blood cells △R and plasma (△R-
A coffin isolate of 1-n, 1-n, and 1-n is easily obtained.

The content rate of white blood cells in blood components collected by the conventional method is W/(ΔR-) W-)-JJ), but in the case of the present invention, it is 4.2ΔRn,W,-f-
tsPi).

The improvement ratio of white blood cell content is expressed by the following formula.

That is, the white blood cell content of the blood components collected according to the present invention increases as the number of times of rough separation increases.

Next, the present invention will be explained in detail with reference to FIGS. 2 and 3.

Blood is collected from the blood donor using the roller pump 19, and the roller pump 25 is adjusted so that the blood anticoagulant 26 is in a uniform ratio. ! 1
One rotation number is set, and energized saline saline 27 is added and stored in one blood tank 21. Roller pump 24? 1O
After pouring an appropriate amount of blood into the centrifugal rotor 3' from the blood sprinkling volume 1 through the opening, the pump 24JI-i is stopped. The blood in the centrifugal rotor 3 forms separation layers in the order of red blood cells 9, blood cells, and plasma 11 from the outer periphery due to the centrifugal force proportional to the square of the rotation number and the radius of rotation.

Next, the roller pump 24 is turned to feed whole blood (not shown) to the outer periphery of the rotor chamber, and at the same time the valve 22 is opened, allowing plasma U to flow out in addition to the blood bag. When the white blood cells 10 come out to the blood outflow port 2, the valve 22 is closed, and when the valve is opened, the white blood cells (white blood cells) flow out into the blood bag 8. Part 22 in response to
Fi is closed. When the red blood cells 9 begin to appear in the blood bag 8, the valve is closed and the rotation of the centrifugal rotor 3 is stopped. The outflow of primary red blood cells 9 is similarly detected by another sensor (not shown), and the rotor 3 stops rotating in response to the sensor.

The remaining blood accumulates in the reservoir 14 with red blood cells in the lower layer and plasma in the upper layer. When the rotor 3 (b) stops rolling, the roller pump 23 immediately rotates and collects the remaining blood in the blood bag, allowing the mixed air to escape to the upper part of the bag 20 and allowing the blood to remain air-free. While appropriately diluting the blood with physiological saline 28, the blood is returned to the donor 15 through the Z filter 29 using the roller pump 6. When the above operation is repeated once, the amount of separated components in the blood bag 8 will be reduced to 9 (△R-)-
W+ΔpL) is accumulated.

Next, the roller pump 7 is turned to return the crudely separated components to the rotor 3', and centrifugation is performed.
6 (9, △R), white blood cells 17 (9, w), plasma 18
(?L, ΔPj) O° order 5 A wide separation layer of white blood cells is obtained. The roller pump 24 pushes whole blood (not shown) from the blood bag 21 into the outer periphery of the centrifugal rotor chamber 9 and the valve 2
2, plasma 18 comes out from the opening 31 into the blood bag. When the white blood cells 17 reach the blood outflow port 2, the valve 22 is closed and the valve 30 is opened to introduce the total amount W of the white blood cells 17 into the blood bag 8 along with a small amount ΔP1 of plasma particles. When the valve (supply) is closed when the blood enters the blood bag 8, it becomes a separated component (ΔR19, W+
Δpz) can be collected.

The roller pumps 19 and 25 are stopped, blood collection from the donor 6 is completed, and dripping of the physiological saline 27 is also stopped, and the roller pump 24 is rotated until the blood bag 21 is empty. When the rotation of the rotor 3' is stopped, the residual blood collects in the reservoir 14, and is guided to the blood bag l using the reroller bomb 23 through the opening, and after removing air bubbles, the roller pump adds physiological saline 28 as necessary. 6 to the donor 15 via the filter 29
4C Return blood. If the blood bag 20 is located sufficiently higher than the donor 6, the roller pump 6 may be omitted.

The following has been explained using the example of white blood cells, but in general, the layer that forms the bucficoat at the boundary between red blood cells and plasma is collected. Strictly speaking, granulocytes and platelets are often required. However, even in that case, the crude separation method and the blood return method are common, and the only difference is the chestnut separation method.

Granulocytes can be isolated by collecting the buffy coat near the red blood cells. According to the prior art, the white blood cell layer is so wide that it is difficult to collect it, but according to the present invention, the layer is expanded by a factor of 1, making the next collection method easier. That is, when whole blood is sent to the outer periphery of the centrifugal rotor chamber by a roller pump and the valve 22 is opened, plasma is pushed out through the opening 31 and into the blood bag. Next, when some white blood cells 17- have entered, the valve 22 is closed and the gap between the valves is opened to send them into the blood bag 8.
When some red blood cells have entered, the valve gap is closed and the roller pump is stopped, and the purified surface liquid component (n, W+?FL, ΔR) containing granulocytes at a high density can be collected.

For fine separation of platelets, it is sufficient to collect blood plasma from platelets. Similarly, when the white blood cells 17 are pushed out to the blood outflow port 2, the valve mold is closed and the valve 30 is opened, and the blood outflow p2
As soon as 16 red blood cells appear, close square 30.

When the roller pump 24 is stopped, the separated blood containing platelets at a high density (?L, W-1-?FL,
ΔPj) can be collected. (?L>?FL) According to the present invention, it is possible not only to easily and reliably collect a blood component containing a high concentration of white blood cells from the whole blood of a blood donor, but also to collect a blood component containing a high concentration of granulocytes. It is easy and reliable to collect things, and it is also possible to collect things that contain platelets at a high density. This means that when a large amount of blood is taken from a donor, only trace amounts of the above blood components are taken out, and the remaining blood is returned to the donor, high efficiency collection reduces the burden on the donor. But the effect is great.

[Brief explanation of the drawing]

FIG. 1 is an explanatory diagram showing a conventional blood component collection method, and FIGS. 2 and 3 are explanatory diagrams showing a blood component collection method according to the present invention. 1 is a blood inlet, 2 is a blood outlet, 3.3' is centrifugal fraction 1
11'-ta, 4 is a seal, 5 is a seal, 6 is a roller pump, 7 is a roller pump, 8 is a blood cell (lug, 9 is a red blood cell, 10 is a white blood cell, 11 is a plasma, Minoru is an opening, 13 is an opening Department, support is Tamabe, 15 blood donors, Jin is red blood cells, 17 are white blood cells, Kakashi is plasma, stand is roller pump, another is blood bag, 2
1Fi blood bag, 22 is valve, n is roller pump, 24
25 is a roller pump, 25 is a blood anticoagulant, 27 is physiological saline, 29 is a filter, 3 o Ifi valve, 31 is an opening. Name of patent applicant Hitachi Koki Co., Ltd.

Claims (1)

[Claims] Blood collected from a donor is injected into the rotor chamber to form a centrifugal separation layer, and the crudely separated blood components containing the blood components to be collected, such as granulocytes or platelets, and some adjacent red blood cells and plasma are transferred to an external container. The blood remaining in the rotor is collected in the rotor, and the blood remaining in the rotor is returned to the donor. This operation is repeated multiple times, and the crudely separated blood components from multiple times are returned to the rotor chamber. The same separation and collection operation is repeated one or more times to form granules. A method for collecting separated blood components containing a high density of blood components to be collected, such as spheres or platelets.