JPS61282085A - ビフエニル代謝機能を備えた外来遺伝子ｂｐｈ Ａ−Ｃ及びこれを保有する新規な微生物 - Google Patents
ビフエニル代謝機能を備えた外来遺伝子ｂｐｈ Ａ−Ｃ及びこれを保有する新規な微生物Info
- Publication number
- JPS61282085A JPS61282085A JP60124555A JP12455585A JPS61282085A JP S61282085 A JPS61282085 A JP S61282085A JP 60124555 A JP60124555 A JP 60124555A JP 12455585 A JP12455585 A JP 12455585A JP S61282085 A JPS61282085 A JP S61282085A
- Authority
- JP
- Japan
- Prior art keywords
- strain
- biphenyl
- bacteria
- bpha
- exogenote
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- ZUOUZKKEUPVFJK-UHFFFAOYSA-N diphenyl Chemical compound C1=CC=CC=C1C1=CC=CC=C1 ZUOUZKKEUPVFJK-UHFFFAOYSA-N 0.000 title claims abstract description 31
- 235000010290 biphenyl Nutrition 0.000 title claims abstract description 15
- 239000004305 biphenyl Substances 0.000 title claims abstract description 15
- 230000007102 metabolic function Effects 0.000 title claims abstract description 5
- 244000005700 microbiome Species 0.000 title claims description 4
- 108090000623 proteins and genes Proteins 0.000 claims abstract description 21
- 108091008146 restriction endonucleases Proteins 0.000 claims abstract description 10
- 241000589630 Pseudomonas pseudoalcaligenes Species 0.000 claims abstract description 4
- 101000702488 Rattus norvegicus High affinity cationic amino acid transporter 1 Proteins 0.000 claims description 3
- 241001099272 Pseudomonas pseudoalcaligenes KF707 = NBRC 110670 Species 0.000 claims 1
- 239000013612 plasmid Substances 0.000 abstract description 9
- 241000894006 Bacteria Species 0.000 abstract description 8
- 241000588724 Escherichia coli Species 0.000 abstract description 5
- 238000003776 cleavage reaction Methods 0.000 abstract description 4
- 230000007017 scission Effects 0.000 abstract description 4
- 241000589517 Pseudomonas aeruginosa Species 0.000 abstract description 3
- 230000003698 anagen phase Effects 0.000 abstract description 3
- 238000005406 washing Methods 0.000 abstract description 3
- 241000193830 Bacillus <bacterium> Species 0.000 abstract description 2
- 241000589776 Pseudomonas putida Species 0.000 abstract description 2
- 238000007796 conventional method Methods 0.000 abstract description 2
- 238000011426 transformation method Methods 0.000 abstract description 2
- 239000007853 buffer solution Substances 0.000 abstract 2
- 239000000725 suspension Substances 0.000 abstract 2
- 239000000872 buffer Substances 0.000 description 6
- 210000004027 cell Anatomy 0.000 description 6
- 230000012010 growth Effects 0.000 description 5
- 238000006243 chemical reaction Methods 0.000 description 4
- 150000001875 compounds Chemical class 0.000 description 4
- 230000002503 metabolic effect Effects 0.000 description 4
- XEKOWRVHYACXOJ-UHFFFAOYSA-N Ethyl acetate Chemical compound CCOC(C)=O XEKOWRVHYACXOJ-UHFFFAOYSA-N 0.000 description 3
- 102000004316 Oxidoreductases Human genes 0.000 description 3
- 108090000854 Oxidoreductases Proteins 0.000 description 3
- KDYFGRWQOYBRFD-UHFFFAOYSA-N Succinic acid Natural products OC(=O)CCC(O)=O KDYFGRWQOYBRFD-UHFFFAOYSA-N 0.000 description 3
- 210000003495 flagella Anatomy 0.000 description 3
- 238000000034 method Methods 0.000 description 3
- 229920001817 Agar Polymers 0.000 description 2
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 description 2
- 108090000790 Enzymes Proteins 0.000 description 2
- 102000004190 Enzymes Human genes 0.000 description 2
- 102000004020 Oxygenases Human genes 0.000 description 2
- 108090000417 Oxygenases Proteins 0.000 description 2
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 2
- 229920002472 Starch Polymers 0.000 description 2
- 239000007983 Tris buffer Substances 0.000 description 2
- 239000002253 acid Substances 0.000 description 2
- 239000008272 agar Substances 0.000 description 2
- 230000001580 bacterial effect Effects 0.000 description 2
- YKOQAAJBYBTSBS-UHFFFAOYSA-N biphenyl-2,3-diol Chemical compound OC1=CC=CC(C=2C=CC=CC=2)=C1O YKOQAAJBYBTSBS-UHFFFAOYSA-N 0.000 description 2
- 238000005119 centrifugation Methods 0.000 description 2
- 239000013611 chromosomal DNA Substances 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 229940088598 enzyme Drugs 0.000 description 2
- 238000000605 extraction Methods 0.000 description 2
- 230000007062 hydrolysis Effects 0.000 description 2
- 238000006460 hydrolysis reaction Methods 0.000 description 2
- 239000000543 intermediate Substances 0.000 description 2
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 230000004060 metabolic process Effects 0.000 description 2
- 239000000049 pigment Substances 0.000 description 2
- 238000012216 screening Methods 0.000 description 2
- 239000000243 solution Substances 0.000 description 2
- 238000010186 staining Methods 0.000 description 2
- 239000008107 starch Substances 0.000 description 2
- 235000019698 starch Nutrition 0.000 description 2
- UCSJYZPVAKXKNQ-HZYVHMACSA-N streptomycin Chemical compound CN[C@H]1[C@H](O)[C@@H](O)[C@H](CO)O[C@H]1O[C@@H]1[C@](C=O)(O)[C@H](C)O[C@H]1O[C@@H]1[C@@H](NC(N)=N)[C@H](O)[C@@H](NC(N)=N)[C@H](O)[C@H]1O UCSJYZPVAKXKNQ-HZYVHMACSA-N 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 2
- XNCSCQSQSGDGES-UHFFFAOYSA-N 2-[2-[bis(carboxymethyl)amino]propyl-(carboxymethyl)amino]acetic acid Chemical compound OC(=O)CN(CC(O)=O)C(C)CN(CC(O)=O)CC(O)=O XNCSCQSQSGDGES-UHFFFAOYSA-N 0.000 description 1
- 241000588986 Alcaligenes Species 0.000 description 1
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- 101710088194 Dehydrogenase Proteins 0.000 description 1
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 1
- 241000588722 Escherichia Species 0.000 description 1
- 241000192125 Firmicutes Species 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- 102000003960 Ligases Human genes 0.000 description 1
- 108090000364 Ligases Proteins 0.000 description 1
- 101710132698 Lysozyme 3 Proteins 0.000 description 1
- 239000007993 MOPS buffer Substances 0.000 description 1
- 102000016943 Muramidase Human genes 0.000 description 1
- 108010014251 Muramidase Proteins 0.000 description 1
- 108010062010 N-Acetylmuramoyl-L-alanine Amidase Proteins 0.000 description 1
- 108010059712 Pronase Proteins 0.000 description 1
- 102000006382 Ribonucleases Human genes 0.000 description 1
- 108010083644 Ribonucleases Proteins 0.000 description 1
- 241000191940 Staphylococcus Species 0.000 description 1
- 230000002378 acidificating effect Effects 0.000 description 1
- 239000008186 active pharmaceutical agent Substances 0.000 description 1
- 239000013543 active substance Substances 0.000 description 1
- 244000052616 bacterial pathogen Species 0.000 description 1
- 239000003899 bactericide agent Substances 0.000 description 1
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 239000001055 blue pigment Substances 0.000 description 1
- 101150022758 bphA gene Proteins 0.000 description 1
- 101150086954 bphB gene Proteins 0.000 description 1
- 101150005170 bphC gene Proteins 0.000 description 1
- KDYFGRWQOYBRFD-NUQCWPJISA-N butanedioic acid Chemical compound O[14C](=O)CC[14C](O)=O KDYFGRWQOYBRFD-NUQCWPJISA-N 0.000 description 1
- 229940041514 candida albicans extract Drugs 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- 239000006285 cell suspension Substances 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 230000009089 cytolysis Effects 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- 239000012634 fragment Substances 0.000 description 1
- 238000002290 gas chromatography-mass spectrometry Methods 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- 238000003306 harvesting Methods 0.000 description 1
- 101150029472 hsaC gene Proteins 0.000 description 1
- 229930027917 kanamycin Natural products 0.000 description 1
- 229960000318 kanamycin Drugs 0.000 description 1
- SBUJHOSQTJFQJX-NOAMYHISSA-N kanamycin Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CN)O[C@@H]1O[C@H]1[C@H](O)[C@@H](O[C@@H]2[C@@H]([C@@H](N)[C@H](O)[C@@H](CO)O2)O)[C@H](N)C[C@@H]1N SBUJHOSQTJFQJX-NOAMYHISSA-N 0.000 description 1
- 229930182823 kanamycin A Natural products 0.000 description 1
- 239000004310 lactic acid Substances 0.000 description 1
- 235000014655 lactic acid Nutrition 0.000 description 1
- 229960000274 lysozyme Drugs 0.000 description 1
- 235000010335 lysozyme Nutrition 0.000 description 1
- 239000004325 lysozyme Substances 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 230000035939 shock Effects 0.000 description 1
- 238000005507 spraying Methods 0.000 description 1
- 229960005322 streptomycin Drugs 0.000 description 1
- 239000001384 succinic acid Substances 0.000 description 1
- 125000000391 vinyl group Chemical group [H]C([*])=C([H])[H] 0.000 description 1
- 229920002554 vinyl polymer Polymers 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
- 239000012138 yeast extract Substances 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/52—Genes encoding for enzymes or proenzymes
Landscapes
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Chemical & Material Sciences (AREA)
- Molecular Biology (AREA)
- Organic Chemistry (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Microbiology (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP60124555A JPS61282085A (ja) | 1985-06-08 | 1985-06-08 | ビフエニル代謝機能を備えた外来遺伝子ｂｐｈ Ａ−Ｃ及びこれを保有する新規な微生物 |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP60124555A JPS61282085A (ja) | 1985-06-08 | 1985-06-08 | ビフエニル代謝機能を備えた外来遺伝子ｂｐｈ Ａ−Ｃ及びこれを保有する新規な微生物 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS61282085A true JPS61282085A (ja) | 1986-12-12 |
| JPH0336515B2 JPH0336515B2 (cs) | 1991-05-31 |
Family
ID=14888376
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP60124555A Granted JPS61282085A (ja) | 1985-06-08 | 1985-06-08 | ビフエニル代謝機能を備えた外来遺伝子ｂｐｈ Ａ−Ｃ及びこれを保有する新規な微生物 |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS61282085A (cs) |
-
1985
- 1985-06-08 JP JP60124555A patent/JPS61282085A/ja active Granted
Also Published As
| Publication number | Publication date |
|---|---|
| JPH0336515B2 (cs) | 1991-05-31 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| O’Sullivan et al. | Large scale production of cyclohexanone monooxygenase from Escherichia coli TOP10 pQR239 | |
| CN103497911A (zh) | 金黄杆菌及其羰基还原酶用于阿瑞匹坦手性中间体生产 | |
| CN109055324B (zh) | 一种改进的酮还原酶及其应用 | |
| CN111454998B (zh) | 一种手性羟基酸酯的生物制备方法 | |
| JP3408737B2 (ja) | ニトリルヒドラターゼの活性化に関与するタンパク質及びそれをコードする遺伝子 | |
| RU2088667C1 (ru) | Способ микробиологического гидроксилирования метильных групп ароматических 5- или 6-членных гетероциклов, рекомбинантная плазмидная днк pl04, рекомбинантная плазмидная днк pl05, штамм микроорганизма escherichia coli, используемый для получения гидроксиметильных производных ароматических 5- или 6-членных гетероциклов, и штамм микроорганизма pseudomonas putida, используемый для получения гидроксиметильных производных ароматических 5- или 6-членных гетероциклов | |
| JP2003531620A (ja) | チモモナスモビリスにおける部位特異的挿入方法 | |
| JP4753273B2 (ja) | 光学活性ピリジンエタノール誘導体の製造方法 | |
| JPH0614771A (ja) | アスコルビン酸の中間体およびプロセス酵素類 | |
| JPS61282085A (ja) | ビフエニル代謝機能を備えた外来遺伝子ｂｐｈ Ａ−Ｃ及びこれを保有する新規な微生物 | |
| CN113249348B (zh) | 羰基还原酶、其基因、含有该基因的重组表达转化体及其应用 | |
| CN111690581B (zh) | 利用重组大肠杆菌发酵生产冰核蛋白的方法 | |
| CN116200352A (zh) | 7α-羟基类固醇脱氢酶突变体及其在7-氧代-石胆酸合成中的应用 | |
| US5308765A (en) | Esterase genes, esterase, recombinant plasmids and transformants containing the recombinant plasmid and methods of producing optically acitve carboxylic acids and their enantiomeric esters using said transformants | |
| US20070015236A1 (en) | Process of preparing prednisolone devoid of steroid-derived impurities using recombinant E. coli transformed with A delta¹-dehydrogenase gene | |
| US20040023348A1 (en) | Microbiological process for enantioselective (S)-hydroxylation | |
| US5306625A (en) | Process for terminal hydroxylation of ethyl groups on aromatic 5- or 6-ring heterocycles | |
| JPH0336510B2 (cs) | ||
| JPH0342071B2 (cs) | ||
| JPH0559705B2 (cs) | ||
| JPS63157986A (ja) | 遺伝子組換によるｌ−フエニルアラニンの製造方法 | |
| FR2464999A1 (fr) | Procede pour edifier un element genetique determinant la production d'un enzyme | |
| JP2598718B2 (ja) | 新規インドール−３−ピルビン酸デカルボキシラーゼおよびその製造法 | |
| JPH0468909B2 (cs) | ||
| CN114480459A (zh) | 氧化酶全细胞催化剂及其制备高光学纯度r型1,3丁二醇的方法 |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| EXPY | Cancellation because of completion of term |