JPS61219861A - オ−トラジオグラフ解析方法 - Google Patents
オ−トラジオグラフ解析方法Info
- Publication number
- JPS61219861A JPS61219861A JP6229885A JP6229885A JPS61219861A JP S61219861 A JPS61219861 A JP S61219861A JP 6229885 A JP6229885 A JP 6229885A JP 6229885 A JP6229885 A JP 6229885A JP S61219861 A JPS61219861 A JP S61219861A
- Authority
- JP
- Japan
- Prior art keywords
- autoradiograph
- separation
- analysis method
- image
- radiolabeled substance
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000004458 analytical method Methods 0.000 title claims abstract description 36
- 238000012545 processing Methods 0.000 claims abstract description 57
- 230000002285 radioactive effect Effects 0.000 claims abstract description 7
- 239000000126 substance Substances 0.000 claims description 63
- 238000000926 separation method Methods 0.000 claims description 59
- 238000000034 method Methods 0.000 claims description 41
- 238000011161 development Methods 0.000 claims description 35
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 claims description 25
- 239000000463 material Substances 0.000 claims description 10
- 102000039446 nucleic acids Human genes 0.000 claims description 5
- 108020004707 nucleic acids Proteins 0.000 claims description 5
- 150000007523 nucleic acids Chemical class 0.000 claims description 5
- 229920001222 biopolymer Polymers 0.000 claims description 3
- 239000002131 composite material Substances 0.000 claims description 3
- 238000000354 decomposition reaction Methods 0.000 claims description 3
- 230000005284 excitation Effects 0.000 claims description 2
- 230000001678 irradiating effect Effects 0.000 claims description 2
- 238000012937 correction Methods 0.000 abstract description 13
- 238000001962 electrophoresis Methods 0.000 abstract description 12
- 239000000203 mixture Substances 0.000 abstract description 3
- 230000005012 migration Effects 0.000 description 23
- 238000013508 migration Methods 0.000 description 19
- 230000005855 radiation Effects 0.000 description 19
- 239000012634 fragment Substances 0.000 description 14
- 238000010586 diagram Methods 0.000 description 7
- 108090000623 proteins and genes Proteins 0.000 description 6
- 238000006243 chemical reaction Methods 0.000 description 5
- 102000004169 proteins and genes Human genes 0.000 description 4
- 238000002601 radiography Methods 0.000 description 4
- 238000000376 autoradiography Methods 0.000 description 3
- 238000002347 injection Methods 0.000 description 3
- 239000007924 injection Substances 0.000 description 3
- 238000012163 sequencing technique Methods 0.000 description 3
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 description 2
- OPTASPLRGRRNAP-UHFFFAOYSA-N cytosine Chemical compound NC=1C=CNC(=O)N=1 OPTASPLRGRRNAP-UHFFFAOYSA-N 0.000 description 2
- 238000000151 deposition Methods 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 230000006870 function Effects 0.000 description 2
- UYTPUPDQBNUYGX-UHFFFAOYSA-N guanine Chemical compound O=C1NC(N)=NC2=C1N=CN2 UYTPUPDQBNUYGX-UHFFFAOYSA-N 0.000 description 2
- 239000012535 impurity Substances 0.000 description 2
- 238000005259 measurement Methods 0.000 description 2
- 239000000941 radioactive substance Substances 0.000 description 2
- 238000012216 screening Methods 0.000 description 2
- 238000004809 thin layer chromatography Methods 0.000 description 2
- RWQNBRDOKXIBIV-UHFFFAOYSA-N thymine Chemical compound CC1=CNC(=O)NC1=O RWQNBRDOKXIBIV-UHFFFAOYSA-N 0.000 description 2
- 229930024421 Adenine Natural products 0.000 description 1
- GFFGJBXGBJISGV-UHFFFAOYSA-N Adenine Chemical compound NC1=NC=NC2=C1N=CN2 GFFGJBXGBJISGV-UHFFFAOYSA-N 0.000 description 1
- 238000012935 Averaging Methods 0.000 description 1
- 229910052693 Europium Inorganic materials 0.000 description 1
- 238000002105 Southern blotting Methods 0.000 description 1
- 229960000643 adenine Drugs 0.000 description 1
- 210000003323 beak Anatomy 0.000 description 1
- 239000004202 carbamide Substances 0.000 description 1
- 229940104302 cytosine Drugs 0.000 description 1
- 230000008021 deposition Effects 0.000 description 1
- -1 europium-activated barium fluoride bromide Chemical class 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 238000001502 gel electrophoresis Methods 0.000 description 1
- 230000002068 genetic effect Effects 0.000 description 1
- 230000017525 heat dissipation Effects 0.000 description 1
- 230000010365 information processing Effects 0.000 description 1
- 229920002521 macromolecule Polymers 0.000 description 1
- 238000004452 microanalysis Methods 0.000 description 1
- 238000004816 paper chromatography Methods 0.000 description 1
- 230000035515 penetration Effects 0.000 description 1
- 239000002985 plastic film Substances 0.000 description 1
- 229920006255 plastic film Polymers 0.000 description 1
- 238000003672 processing method Methods 0.000 description 1
- 230000001681 protective effect Effects 0.000 description 1
- 229940113082 thymine Drugs 0.000 description 1
- 238000004454 trace mineral analysis Methods 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
- 238000001262 western blot Methods 0.000 description 1
Landscapes
- Investigating Or Analysing Biological Materials (AREA)
Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP6229885A JPS61219861A (ja) | 1985-03-27 | 1985-03-27 | オ−トラジオグラフ解析方法 |
| US06/844,967 US4837687A (en) | 1985-03-27 | 1986-03-27 | Method for analyzing an autoradiograph |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP6229885A JPS61219861A (ja) | 1985-03-27 | 1985-03-27 | オ−トラジオグラフ解析方法 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS61219861A true JPS61219861A (ja) | 1986-09-30 |
| JPH0570111B2 JPH0570111B2 (enrdf_load_stackoverflow) | 1993-10-04 |
Family
ID=13196071
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP6229885A Granted JPS61219861A (ja) | 1985-03-27 | 1985-03-27 | オ−トラジオグラフ解析方法 |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS61219861A (enrdf_load_stackoverflow) |
-
1985
- 1985-03-27 JP JP6229885A patent/JPS61219861A/ja active Granted
Also Published As
| Publication number | Publication date |
|---|---|
| JPH0570111B2 (enrdf_load_stackoverflow) | 1993-10-04 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| LAPS | Cancellation because of no payment of annual fees |