JPH0529069B2 - - Google Patents
Info
- Publication number
- JPH0529069B2 JPH0529069B2 JP5548386A JP5548386A JPH0529069B2 JP H0529069 B2 JPH0529069 B2 JP H0529069B2 JP 5548386 A JP5548386 A JP 5548386A JP 5548386 A JP5548386 A JP 5548386A JP H0529069 B2 JPH0529069 B2 JP H0529069B2
- Authority
- JP
- Japan
- Prior art keywords
- autoradiograph
- band
- base sequence
- cursor
- nucleic acid
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 238000000034 method Methods 0.000 claims description 48
- 238000004458 analytical method Methods 0.000 claims description 34
- 150000007523 nucleic acids Chemical class 0.000 claims description 33
- 102000039446 nucleic acids Human genes 0.000 claims description 32
- 108020004707 nucleic acids Proteins 0.000 claims description 32
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 claims description 28
- 238000011161 development Methods 0.000 claims description 28
- 239000012634 fragment Substances 0.000 claims description 26
- 238000000926 separation method Methods 0.000 claims description 25
- 239000000126 substance Substances 0.000 claims description 22
- 238000012545 processing Methods 0.000 claims description 21
- 238000012790 confirmation Methods 0.000 claims description 13
- 239000000463 material Substances 0.000 claims description 11
- 230000002285 radioactive effect Effects 0.000 claims description 9
- 238000012937 correction Methods 0.000 claims description 7
- 230000005284 excitation Effects 0.000 claims description 3
- 230000001678 irradiating effect Effects 0.000 claims description 3
- 238000012163 sequencing technique Methods 0.000 claims 2
- 238000001962 electrophoresis Methods 0.000 description 24
- 238000013508 migration Methods 0.000 description 20
- 230000005012 migration Effects 0.000 description 20
- 239000000203 mixture Substances 0.000 description 13
- 230000005855 radiation Effects 0.000 description 13
- 238000010586 diagram Methods 0.000 description 10
- 235000004522 Pentaglottis sempervirens Nutrition 0.000 description 7
- OPTASPLRGRRNAP-UHFFFAOYSA-N cytosine Chemical compound NC=1C=CNC(=O)N=1 OPTASPLRGRRNAP-UHFFFAOYSA-N 0.000 description 6
- UYTPUPDQBNUYGX-UHFFFAOYSA-N guanine Chemical compound O=C1NC(N)=NC2=C1N=CN2 UYTPUPDQBNUYGX-UHFFFAOYSA-N 0.000 description 6
- 230000008569 process Effects 0.000 description 6
- 240000004050 Pentaglottis sempervirens Species 0.000 description 4
- 238000006243 chemical reaction Methods 0.000 description 4
- 238000003776 cleavage reaction Methods 0.000 description 4
- 230000007017 scission Effects 0.000 description 4
- RWQNBRDOKXIBIV-UHFFFAOYSA-N thymine Chemical compound CC1=CNC(=O)NC1=O RWQNBRDOKXIBIV-UHFFFAOYSA-N 0.000 description 4
- GFFGJBXGBJISGV-UHFFFAOYSA-N Adenine Chemical compound NC1=NC=NC2=C1N=CN2 GFFGJBXGBJISGV-UHFFFAOYSA-N 0.000 description 3
- 229930024421 Adenine Natural products 0.000 description 3
- 229960000643 adenine Drugs 0.000 description 3
- 229940104302 cytosine Drugs 0.000 description 3
- 230000007018 DNA scission Effects 0.000 description 2
- ISAKRJDGNUQOIC-UHFFFAOYSA-N Uracil Chemical compound O=C1C=CNC(=O)N1 ISAKRJDGNUQOIC-UHFFFAOYSA-N 0.000 description 2
- 150000001875 compounds Chemical class 0.000 description 2
- 230000007547 defect Effects 0.000 description 2
- 239000007857 degradation product Substances 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 230000002068 genetic effect Effects 0.000 description 2
- 238000003672 processing method Methods 0.000 description 2
- 239000000047 product Substances 0.000 description 2
- 238000002601 radiography Methods 0.000 description 2
- 229940113082 thymine Drugs 0.000 description 2
- 238000012935 Averaging Methods 0.000 description 1
- 230000006820 DNA synthesis Effects 0.000 description 1
- 229910052693 Europium Inorganic materials 0.000 description 1
- 238000000376 autoradiography Methods 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 238000004440 column chromatography Methods 0.000 description 1
- 230000000295 complement effect Effects 0.000 description 1
- 239000002131 composite material Substances 0.000 description 1
- 239000000470 constituent Substances 0.000 description 1
- 238000000354 decomposition reaction Methods 0.000 description 1
- -1 europium-activated barium fluoride bromide Chemical class 0.000 description 1
- 230000006870 function Effects 0.000 description 1
- 238000001502 gel electrophoresis Methods 0.000 description 1
- 230000017525 heat dissipation Effects 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 238000004816 paper chromatography Methods 0.000 description 1
- 239000002985 plastic film Substances 0.000 description 1
- 229920006255 plastic film Polymers 0.000 description 1
- 229920000642 polymer Polymers 0.000 description 1
- 230000001681 protective effect Effects 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 230000010076 replication Effects 0.000 description 1
- 230000002194 synthesizing effect Effects 0.000 description 1
- 238000004809 thin layer chromatography Methods 0.000 description 1
- 235000011178 triphosphate Nutrition 0.000 description 1
- 239000001226 triphosphate Substances 0.000 description 1
- UNXRWKVEANCORM-UHFFFAOYSA-N triphosphoric acid Chemical compound OP(O)(=O)OP(O)(=O)OP(O)(O)=O UNXRWKVEANCORM-UHFFFAOYSA-N 0.000 description 1
- 229940035893 uracil Drugs 0.000 description 1
Landscapes
- Measurement Of Radiation (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Priority Applications (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP61055483A JPS62215869A (ja) | 1986-03-12 | 1986-03-12 | 核酸の塩基配列決定のためのオ−トラジオグラフ解析方法 |
| EP87103138A EP0240729A3 (en) | 1986-03-05 | 1987-03-05 | Method of analyzing autoradiograph for determining base sequence of nucleic acid |
| US07/420,603 US4982326A (en) | 1986-03-05 | 1989-10-04 | Method for analyzing autoradiograph for determining base sequence of nucleic acid |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP61055483A JPS62215869A (ja) | 1986-03-12 | 1986-03-12 | 核酸の塩基配列決定のためのオ−トラジオグラフ解析方法 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS62215869A JPS62215869A (ja) | 1987-09-22 |
| JPH0529069B2 true JPH0529069B2 (enrdf_load_stackoverflow) | 1993-04-28 |
Family
ID=12999867
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP61055483A Granted JPS62215869A (ja) | 1986-03-05 | 1986-03-12 | 核酸の塩基配列決定のためのオ−トラジオグラフ解析方法 |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS62215869A (enrdf_load_stackoverflow) |
-
1986
- 1986-03-12 JP JP61055483A patent/JPS62215869A/ja active Granted
Also Published As
| Publication number | Publication date |
|---|---|
| JPS62215869A (ja) | 1987-09-22 |