JPS61180141A - 免疫拡散による蛋白の定量分析方法 - Google Patents
免疫拡散による蛋白の定量分析方法Info
- Publication number
- JPS61180141A JPS61180141A JP60156185A JP15618585A JPS61180141A JP S61180141 A JPS61180141 A JP S61180141A JP 60156185 A JP60156185 A JP 60156185A JP 15618585 A JP15618585 A JP 15618585A JP S61180141 A JPS61180141 A JP S61180141A
- Authority
- JP
- Japan
- Prior art keywords
- zone
- protein
- sedimentation zone
- parameter
- sample
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 102000004169 proteins and genes Human genes 0.000 title claims description 253
- 108090000623 proteins and genes Proteins 0.000 title claims description 253
- 238000000034 method Methods 0.000 title claims description 126
- 230000000951 immunodiffusion Effects 0.000 title claims description 44
- 238000004062 sedimentation Methods 0.000 claims description 121
- 239000000427 antigen Substances 0.000 claims description 74
- 102000036639 antigens Human genes 0.000 claims description 74
- 108091007433 antigens Proteins 0.000 claims description 74
- 238000004458 analytical method Methods 0.000 claims description 37
- 230000001965 increasing effect Effects 0.000 claims description 19
- 238000004445 quantitative analysis Methods 0.000 claims description 19
- 230000005856 abnormality Effects 0.000 claims description 17
- 230000008859 change Effects 0.000 claims description 17
- 238000009792 diffusion process Methods 0.000 claims description 17
- 230000003287 optical effect Effects 0.000 claims description 13
- 238000013213 extrapolation Methods 0.000 claims description 12
- 230000004044 response Effects 0.000 claims description 11
- 238000009795 derivation Methods 0.000 claims description 7
- 239000013049 sediment Substances 0.000 claims description 6
- 230000007423 decrease Effects 0.000 claims description 4
- 238000013508 migration Methods 0.000 claims description 4
- 230000005012 migration Effects 0.000 claims description 4
- 238000010186 staining Methods 0.000 claims description 4
- 239000000376 reactant Substances 0.000 claims description 3
- 238000011002 quantification Methods 0.000 claims 1
- 238000005259 measurement Methods 0.000 description 110
- 239000000523 sample Substances 0.000 description 63
- 238000001962 electrophoresis Methods 0.000 description 26
- 239000000243 solution Substances 0.000 description 26
- 238000011534 incubation Methods 0.000 description 22
- 238000000760 immunoelectrophoresis Methods 0.000 description 17
- 230000000875 corresponding effect Effects 0.000 description 16
- 238000004364 calculation method Methods 0.000 description 12
- 230000002159 abnormal effect Effects 0.000 description 11
- 238000001556 precipitation Methods 0.000 description 11
- 238000012937 correction Methods 0.000 description 10
- 230000005684 electric field Effects 0.000 description 10
- 239000000499 gel Substances 0.000 description 10
- 230000037230 mobility Effects 0.000 description 10
- 230000008901 benefit Effects 0.000 description 9
- 230000033001 locomotion Effects 0.000 description 9
- 238000006243 chemical reaction Methods 0.000 description 8
- 238000010586 diagram Methods 0.000 description 8
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- 238000009826 distribution Methods 0.000 description 7
- 238000005286 illumination Methods 0.000 description 7
- 238000012545 processing Methods 0.000 description 7
- 210000003323 beak Anatomy 0.000 description 6
- 239000012530 fluid Substances 0.000 description 6
- 239000007788 liquid Substances 0.000 description 6
- 230000007246 mechanism Effects 0.000 description 6
- 239000000203 mixture Substances 0.000 description 6
- 239000002244 precipitate Substances 0.000 description 6
- 230000000984 immunochemical effect Effects 0.000 description 5
- 229920000936 Agarose Polymers 0.000 description 4
- 201000010099 disease Diseases 0.000 description 4
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 4
- 238000005194 fractionation Methods 0.000 description 4
- 230000001939 inductive effect Effects 0.000 description 4
- 238000000691 measurement method Methods 0.000 description 4
- 210000002966 serum Anatomy 0.000 description 4
- 238000012935 Averaging Methods 0.000 description 3
- 101710100170 Unknown protein Proteins 0.000 description 3
- 230000015572 biosynthetic process Effects 0.000 description 3
- 108010074605 gamma-Globulins Proteins 0.000 description 3
- 239000010410 layer Substances 0.000 description 3
- 230000001376 precipitating effect Effects 0.000 description 3
- 230000002123 temporal effect Effects 0.000 description 3
- FTOAOBMCPZCFFF-UHFFFAOYSA-N 5,5-diethylbarbituric acid Chemical compound CCC1(CC)C(=O)NC(=O)NC1=O FTOAOBMCPZCFFF-UHFFFAOYSA-N 0.000 description 2
- 229920001817 Agar Polymers 0.000 description 2
- 108060003951 Immunoglobulin Proteins 0.000 description 2
- 239000002253 acid Substances 0.000 description 2
- 239000008272 agar Substances 0.000 description 2
- 239000003153 chemical reaction reagent Substances 0.000 description 2
- 238000004587 chromatography analysis Methods 0.000 description 2
- 238000012733 comparative method Methods 0.000 description 2
- 238000004590 computer program Methods 0.000 description 2
- 230000002596 correlated effect Effects 0.000 description 2
- 230000003247 decreasing effect Effects 0.000 description 2
- 230000001419 dependent effect Effects 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 238000005370 electroosmosis Methods 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 239000000284 extract Substances 0.000 description 2
- 238000007429 general method Methods 0.000 description 2
- 230000036541 health Effects 0.000 description 2
- 102000018358 immunoglobulin Human genes 0.000 description 2
- 230000036046 immunoreaction Effects 0.000 description 2
- 238000000338 in vitro Methods 0.000 description 2
- 238000002347 injection Methods 0.000 description 2
- 239000007924 injection Substances 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 239000012460 protein solution Substances 0.000 description 2
- 238000004451 qualitative analysis Methods 0.000 description 2
- 238000013214 routine measurement Methods 0.000 description 2
- 239000012488 sample solution Substances 0.000 description 2
- 229920006395 saturated elastomer Polymers 0.000 description 2
- 238000000926 separation method Methods 0.000 description 2
- 239000002356 single layer Substances 0.000 description 2
- 239000002904 solvent Substances 0.000 description 2
- 238000010561 standard procedure Methods 0.000 description 2
- 239000012086 standard solution Substances 0.000 description 2
- 230000003068 static effect Effects 0.000 description 2
- 239000013589 supplement Substances 0.000 description 2
- 230000001360 synchronised effect Effects 0.000 description 2
- 238000012546 transfer Methods 0.000 description 2
- 238000005406 washing Methods 0.000 description 2
- 102000008100 Human Serum Albumin Human genes 0.000 description 1
- 108091006905 Human Serum Albumin Proteins 0.000 description 1
- 101150062967 PHOX2A gene Proteins 0.000 description 1
- BQCADISMDOOEFD-UHFFFAOYSA-N Silver Chemical compound [Ag] BQCADISMDOOEFD-UHFFFAOYSA-N 0.000 description 1
- 239000011358 absorbing material Substances 0.000 description 1
- 230000004931 aggregating effect Effects 0.000 description 1
- 229960002319 barbital Drugs 0.000 description 1
- 239000007982 barbital buffer Substances 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- -1 commonly used today Polymers 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 238000001114 immunoprecipitation Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000000465 moulding Methods 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 229910052709 silver Inorganic materials 0.000 description 1
- 239000004332 silver Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
Landscapes
- Investigating Or Analysing Biological Materials (AREA)
- Investigating Or Analysing Materials By Optical Means (AREA)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP60156185A JPS61180141A (ja) | 1985-07-17 | 1985-07-17 | 免疫拡散による蛋白の定量分析方法 |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP60156185A JPS61180141A (ja) | 1985-07-17 | 1985-07-17 | 免疫拡散による蛋白の定量分析方法 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS61180141A true JPS61180141A (ja) | 1986-08-12 |
| JPS6255104B2 JPS6255104B2 (enrdf_load_stackoverflow) | 1987-11-18 |
Family
ID=15622220
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP60156185A Granted JPS61180141A (ja) | 1985-07-17 | 1985-07-17 | 免疫拡散による蛋白の定量分析方法 |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS61180141A (enrdf_load_stackoverflow) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH04339259A (ja) * | 1989-12-05 | 1992-11-26 | Delta Biotechnol Ltd | 突然変異菌株の検出法 |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS54137399A (en) * | 1978-04-14 | 1979-10-25 | Jiei Arajiemu Furederitsuku | Method and device for analyzing quantity of protein by immunological electrophoresis |
-
1985
- 1985-07-17 JP JP60156185A patent/JPS61180141A/ja active Granted
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS54137399A (en) * | 1978-04-14 | 1979-10-25 | Jiei Arajiemu Furederitsuku | Method and device for analyzing quantity of protein by immunological electrophoresis |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH04339259A (ja) * | 1989-12-05 | 1992-11-26 | Delta Biotechnol Ltd | 突然変異菌株の検出法 |
Also Published As
| Publication number | Publication date |
|---|---|
| JPS6255104B2 (enrdf_load_stackoverflow) | 1987-11-18 |
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