JPS61177413A - Heating and cooling device of microscope - Google Patents

Abstract

PURPOSE:To reduce thermal deformation in case of heating or cooling by making the sectional shape in the direction perpendicular to the optical axis of a plate-shaped member uneven in the direction of the optical axis. CONSTITUTION:This device consists of the plate-shaped member which is arranged vertically with respect to the optical axis and has one face, to which a heating body and/or a cooling body are joined, at least, and the sectional shape in the direction perpendicular to the optical axis of the plate-shaped member is made uneven in the direction of the optical axis. When power is supplied to a film heater 13 to heat a midseat heater 3 and an incubation observing vessel 4 is controlled to a set temperature, the lower face, which is brought into contact with the film heater 13 of a disc 11, of the heater 3 has a temperature higher than that of the upper face of the disc 11 and is more expanded thermally therefore, but the stress due to the difference of thermal expansion between the upper face and the lower face of the disc 11 is concentrated to two ring-shaped grooves 11b and 11c provided on the lower face of the disc 11, and thermal distortion is absorbed by ring-shaped grooves, and thus, downward deformation of the disc 11 is reduced.

Description

[Detailed description of the invention] 佔亙ますさら The present invention relates to a heating and cooling device for a microscope.

In general, as a method for controlling a plate-shaped member to a desired temperature, a method is often used in which a heat source such as a heater is brought into contact with the plate-shaped member as shown in FIG. The member H is a heat source, such as a heater, which is joined to the lower surface of the plate-like member P by an appropriate method. When the heat source H is energized from the state shown in FIG. As a result, the plate-like member P deforms as shown in Fig. 3 CB). Particularly when the heat source H is controlled on and off to maintain the plate-like member P at a desired constant temperature, as shown in Fig. 4. Periodic on/off operation of heat source H (A
), the plate member P deforms periodically as shown in FIG. 4(B). Such deformation of the plate-like member P causes blurring of the observed image, for example, when the plate-like member is a stage for mounting a culture specimen provided on the stage of a microscope. In order to prevent this, the amount of blur can be reduced by increasing the thickness of the plate member P. However, when the thickness is increased, the stage Sf) moves;
When rotating the revolver or replacing objective lens 0, objective lens 0 and stage P may easily interfere (see Figure 5 (A)), or the top surface of stage P may be lower than the top surface of stage S. (See Figure 5 (B)) For example, (Figure 5 (C)
Attachment A for moving the specimen (as shown in Figure 1) may become unusable, and in the case of an upright microscope, condenser lens C and stage P may easily interfere (
(See Figure 5 (D)).

Note that the above problem also occurs when a thermo module is used as a heat source to cool a plate member or a stage.

1-Yutaka In view of the above points, it is an object of the present invention to provide a heating/cooling device for a microscope that reduces thermal deformation during heating or cooling without increasing the thickness.

l This purpose consists of a plate-like member arranged perpendicularly to the optical axis and to which a heating element and/or a cooling element are joined on at least one surface, and which is arranged perpendicularly to the optical axis. This problem is solved by a heating/cooling device for a microscope, which is characterized in that the cross-sectional shape in the direction perpendicular to the optical axis is non-uniform.

, 11 Figure 1 shows an inverted microscope equipped with a heating and cooling device according to the present invention, where 1 is an inverted microscope, 2 is a horizontally movable stage, and 3 is a part of stage 2. 4 is a culture observation container placed on the middle seat heater 3; 5 is an objective lens attached to a revolver; This is a temperature controller for controlling the seat heater 3 on and off to a set temperature. Furthermore, the middle seat heater 3 has an observation opening 11 in the center as shown in FIG.
It is composed of an aluminum disk 11 having a radius of
is formed, and a temperature sensor 12 is built-in. A film heater 13 is bonded to the lower surface of the disk 11, and is controlled on and off to a set temperature by a temperature controller 6 based on a signal from a temperature sensor 12. 14
is a cover provided to protect the film heater 13.

Since the embodiment of the present invention is constructed as described above, by energizing the film heater 13, the middle seat heater 3
When the culture observation container 4 is controlled to the set temperature by heating, the lower surface of the disc 11 of the intermediate heater 3 that is in contact with the film heater 13 has a higher temperature than the upper surface of the disc 11, and therefore has a large thermal expansion. However, the stress due to the difference in thermal expansion between the upper and lower surfaces of the disk 11 is concentrated in the two annular grooves 11b and 11C provided on the lower surface of the disk 11, and the thermal strain is absorbed by the annular grooves. , vertical deformation of the disc 11 is reduced.

To show a specific experimental example below, when using the intermediate heater 3 with the dimensions shown in FIG. 6(A), the film heater 1
30 Distance r from the center of the disk 11 synchronized with on/off control
The amount of upward displacement in the two directions (wm) is shown by the solid line in FIG. is decreasing.

Therefore, vertical displacement of the stage due to on/off control of the film heater 13 is reduced, and fluctuations in the distance between the culture observation container 4 and the objective lens 5 are also reduced, thus reducing image blurring. In addition, in FIG. 6(B), the chain line A is 40
The depth of focus (approximately 1.5 μ) is shown for the objective lens marked with ×, and the amount of displacement of the seat heater 3 is within the depth of focus.

7 and 8 show other embodiments of the present invention,
Disc 11 with annular grooves 11b and llc in FIG. 2(B)
A disk 15 (which is used instead of
(see FIG. 7) or a disc 16 (see FIG.
8) is shown. According to these embodiments, the stress due to the difference in thermal expansion is applied to the recess 15a or the arcuate groove 16a.
, 16b, the thermal strain is absorbed by the recesses or grooves, as in the embodiment of FIG. 2, and the vertical deformation of the disk 15 or 16 is reduced.

1 As described above, according to the present invention, it is composed of a plate-like member arranged perpendicularly to the optical axis and having a heating element and/or a cooling element joined to at least one surface. Since the heating and cooling device of the microscope is configured so that the cross-sectional shape of the plate-like member in the direction perpendicular to the optical axis is non-uniform in the optical axis direction, the plate-like member is heated or cooled. The stress generated by the difference in thermal expansion due to the temperature difference that occurs in the plate is concentrated in the non-uniform portion, that is, the groove or the recess, and as a result, the thermal strain is absorbed in this portion, thus reducing the vertical deformation of the plate member. However, since there is no need to increase the thickness in the optical axis direction, it does not affect observation.

Incidentally, in the embodiment, the case where heating is performed by a film heater has been described, but it goes without saying that the present invention can also be applied to a case where cooling is performed by a thermo module.

[Brief explanation of the drawing]

Fig. 1 is a schematic side view of an inverted microscope equipped with a heating/cooling device according to the present invention, and Fig. 2 shows an embodiment of the present invention.
A) Partially cutaway side view, (B) Bottom view of the disc, Figure 3 is a schematic diagram illustrating thermal deformation of the plate member, Figure 4 is the displacement of the plate member in Figure 3 during on/off control. FIG. 5 is a time chart showing the amount, FIG. A) A diagram showing dimensions and (B) a displacement amount due to thermal deformation, and FIGS. 7 and 8 are diagrams showing other embodiments of the present invention. 1... Inverted microscope, 2... Stage, 3...
...Nakaza heater, 4...Culture observation container, 5...
・Objective lens, 6... Temperature controller, 11, 1
5゜16... Disc, 11b, 11C... Annular groove, 12... Temperature sensor, 13... Film heater, 14... Cover, 15a... Circular recess , 16a. 16b...Arc-shaped groove. Spear 2 (B) (A) (B),? 5W
J (A) (D)

Claims (1)

[Claims] Consisting of a plate-shaped member arranged perpendicular to the optical axis and having a heating element and/or cooling element joined to at least one surface, the direction perpendicular to the optical axis of the plate-shaped member A heating and cooling device for a microscope, characterized in that its cross-sectional shape is non-uniform in the optical axis direction.