JPS61100191A - 細胞培養方法 - Google Patents
細胞培養方法Info
- Publication number
- JPS61100191A JPS61100191A JP22121984A JP22121984A JPS61100191A JP S61100191 A JPS61100191 A JP S61100191A JP 22121984 A JP22121984 A JP 22121984A JP 22121984 A JP22121984 A JP 22121984A JP S61100191 A JPS61100191 A JP S61100191A
- Authority
- JP
- Japan
- Prior art keywords
- culture
- filter
- suspension
- cells
- tank
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000004113 cell culture Methods 0.000 title claims abstract description 30
- 239000007788 liquid Substances 0.000 claims abstract description 25
- 239000000725 suspension Substances 0.000 claims abstract description 23
- 230000002411 adverse Effects 0.000 claims description 4
- 230000000694 effects Effects 0.000 claims description 3
- 239000012466 permeate Substances 0.000 claims description 2
- 238000001914 filtration Methods 0.000 abstract description 5
- 238000007599 discharging Methods 0.000 abstract description 3
- 210000004027 cell Anatomy 0.000 description 41
- 238000000034 method Methods 0.000 description 17
- 239000001963 growth medium Substances 0.000 description 13
- 238000012258 culturing Methods 0.000 description 10
- 239000012736 aqueous medium Substances 0.000 description 7
- 239000002609 medium Substances 0.000 description 6
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 6
- 238000012136 culture method Methods 0.000 description 5
- 239000007789 gas Substances 0.000 description 5
- 235000015097 nutrients Nutrition 0.000 description 5
- 239000011148 porous material Substances 0.000 description 5
- 238000010586 diagram Methods 0.000 description 4
- 238000005516 engineering process Methods 0.000 description 4
- 239000000047 product Substances 0.000 description 4
- 238000003756 stirring Methods 0.000 description 4
- 210000002966 serum Anatomy 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- 239000002699 waste material Substances 0.000 description 3
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 2
- 239000000654 additive Substances 0.000 description 2
- 150000001413 amino acids Chemical class 0.000 description 2
- 239000003242 anti bacterial agent Substances 0.000 description 2
- 229940088710 antibiotic agent Drugs 0.000 description 2
- UBAZGMLMVVQSCD-UHFFFAOYSA-N carbon dioxide;molecular oxygen Chemical compound O=O.O=C=O UBAZGMLMVVQSCD-UHFFFAOYSA-N 0.000 description 2
- 150000001875 compounds Chemical class 0.000 description 2
- 239000011521 glass Substances 0.000 description 2
- 239000008103 glucose Substances 0.000 description 2
- 210000004408 hybridoma Anatomy 0.000 description 2
- 239000012528 membrane Substances 0.000 description 2
- 230000035699 permeability Effects 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 102000004169 proteins and genes Human genes 0.000 description 2
- 150000003839 salts Chemical class 0.000 description 2
- 108091003079 Bovine Serum Albumin Proteins 0.000 description 1
- 206010011878 Deafness Diseases 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 206010035226 Plasma cell myeloma Diseases 0.000 description 1
- 239000012980 RPMI-1640 medium Substances 0.000 description 1
- 230000000996 additive effect Effects 0.000 description 1
- 210000004102 animal cell Anatomy 0.000 description 1
- 210000000628 antibody-producing cell Anatomy 0.000 description 1
- 239000003443 antiviral agent Substances 0.000 description 1
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 1
- 230000000903 blocking effect Effects 0.000 description 1
- 230000001413 cellular effect Effects 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 239000005515 coenzyme Substances 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 238000000855 fermentation Methods 0.000 description 1
- 230000004151 fermentation Effects 0.000 description 1
- 239000012091 fetal bovine serum Substances 0.000 description 1
- 239000005556 hormone Substances 0.000 description 1
- 229940088597 hormone Drugs 0.000 description 1
- 238000009776 industrial production Methods 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 229940079322 interferon Drugs 0.000 description 1
- 239000002075 main ingredient Substances 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 239000000155 melt Substances 0.000 description 1
- 230000002503 metabolic effect Effects 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 239000003094 microcapsule Substances 0.000 description 1
- 201000000050 myeloid neoplasm Diseases 0.000 description 1
- 230000002062 proliferating effect Effects 0.000 description 1
- 230000035755 proliferation Effects 0.000 description 1
- 238000013341 scale-up Methods 0.000 description 1
- 239000012679 serum free medium Substances 0.000 description 1
- 239000008223 sterile water Substances 0.000 description 1
- 238000004114 suspension culture Methods 0.000 description 1
- 238000000108 ultra-filtration Methods 0.000 description 1
- 229960005486 vaccine Drugs 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP22121984A JPS61100191A (ja) | 1984-10-23 | 1984-10-23 | 細胞培養方法 |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP22121984A JPS61100191A (ja) | 1984-10-23 | 1984-10-23 | 細胞培養方法 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS61100191A true JPS61100191A (ja) | 1986-05-19 |
| JPH0373274B2 JPH0373274B2 (enrdf_load_stackoverflow) | 1991-11-21 |
Family
ID=16763329
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP22121984A Granted JPS61100191A (ja) | 1984-10-23 | 1984-10-23 | 細胞培養方法 |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS61100191A (enrdf_load_stackoverflow) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105210866A (zh) * | 2015-09-29 | 2016-01-06 | 中国科学院植物研究所 | 一种间歇浸没式兰科植物类原球茎增殖快繁方法 |
| CN105340733A (zh) * | 2015-09-29 | 2016-02-24 | 中国科学院植物研究所 | 一种间歇浸没式苦苣苔叶片不定芽诱导方法 |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS5564795A (en) * | 1978-11-10 | 1980-05-15 | Olympus Optical Co Ltd | Method and apparatus for culturing living tissue or cell |
| JPS5765180A (en) * | 1980-06-20 | 1982-04-20 | Monsanto Co | Method and apparatus for culturing cell |
-
1984
- 1984-10-23 JP JP22121984A patent/JPS61100191A/ja active Granted
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS5564795A (en) * | 1978-11-10 | 1980-05-15 | Olympus Optical Co Ltd | Method and apparatus for culturing living tissue or cell |
| JPS5765180A (en) * | 1980-06-20 | 1982-04-20 | Monsanto Co | Method and apparatus for culturing cell |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105210866A (zh) * | 2015-09-29 | 2016-01-06 | 中国科学院植物研究所 | 一种间歇浸没式兰科植物类原球茎增殖快繁方法 |
| CN105340733A (zh) * | 2015-09-29 | 2016-02-24 | 中国科学院植物研究所 | 一种间歇浸没式苦苣苔叶片不定芽诱导方法 |
Also Published As
| Publication number | Publication date |
|---|---|
| JPH0373274B2 (enrdf_load_stackoverflow) | 1991-11-21 |
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