JPS6035400B2 - Antibacterial cleaning composition - Google Patents

Description

DETAILED DESCRIPTION OF THE INVENTION The present invention provides an antibacterial cleaning composition, more specifically, an antibacterial cleaning composition that sterilizes, cleans and removes mold and bacteria growing on solid surfaces, and prevents their generation. relating to things.

In recent years, as the number of highly airtight homes has increased, mold and bacteria have grown in relatively humid areas such as bathrooms and kitchens, and inside refrigerators, resulting in unsanitary conditions.

Particularly in refrigerators and carpets, mold and bacteria often grow as food juices, dirt, and other food sources serve as nutrients. The growth of mold and bacteria not only spoils the aesthetics and causes bad odors, but mold spores can also become an allergen for childhood bronchial asthma, and food contaminated with bacteria in the refrigerator can cause food poisoning. There are many examples of this being the cause.

In order to eliminate the damage caused by mold and bacteria and maintain a sanitary environment, for example, it is necessary to kill the existing mold and bacteria, suppress the growth of mold and bacteria over a long period of time, or provide nutrients for mold and bacteria. It is necessary to remove the source of dirt.

Conventionally, various disinfectants, fungicides, cleaning, etc. have been proposed as these means.

However, until now, there has been no known product that can simultaneously perform sterilization, anti-fungal, and cleaning activities, or that maintains anti-fungal effects for a long period of time. The present inventors have conducted extensive research to develop a composition that can simultaneously perform sterilization, fungicide, and cleaning, and that maintains its effectiveness for a long period of time. 2-(4-thiazolyl)penzimidazo-, which is known as an antibacterial substance from
The inventors found that the objective could be achieved by adding the most chain alkyldimethylbenzyl ammonium salt to the compound, and based on this knowledge, they completed the present invention.

That is, the present invention provides a nonionic surfactant, 2-(4
-thiazolyl)penzimidazole and a long-chain alkyldimethylbenzylammonium salt.

The nonionic surfactant used in the present invention is a water-soluble nonionic surfactant, such as polyoxyethylene alkyl ether, polyoxyethylene alkyl phenyl ether, glycerin fatty acid ester, sorbitan fatty acid ester, etc. Preferred are polyoxyethylene alkyl ethers.

The amount used can vary widely, as the composition may be used as is or diluted, but is usually 0.1 to 20%, preferably 0.5% based on the weight of the composition. It is in the range of ~5%. The long-chain alkyldimethylbenzylammonium salt (hereinafter abbreviated as AMB) used in the present invention has 10 to 2 carbon atoms as a long-chain alkyl group.
0 is preferred, and an example of this is penzalkonium chloride. 2-(4-thiazolyl)penzimidazole (hereinafter abbreviated as T spots) and AMB used in the present invention
Both are antibacterial and antifungal compounds, and by using them together, they become effective against a wide range of bacteria, and the concentration used can be lowered.

The ratio of TBZ and AMB used in the present invention is 9:1 to 1:9.
, preferably in the range of 4:1 to 1:4.

Further, the total amount of TBZ and AM used is in the range of 0.1 to 2% based on the weight of the composition. If the amount is less than 0.1%, the antibacterial and antifungal effects will be insufficient, and if it exceeds 2%, it will be difficult to form a uniform solution. Also, TBZ
Because of its reluctance to water, a water-lower alcohol mixed solvent is usually used in the composition.

Lower alcohols include monohydric alcohols such as methanol, ethanol, propanol, and isopropanol;
and polyhydric alcohols such as ethylene glycol, propylene glycol, and glycerin, preferably ethanol, which may also be used in combination with an appropriate amount of polyhydric alcohol. The antibacterial cleaning composition of the present invention is a drug that can simultaneously perform sterilization and antifungal cleaning that is not seen in conventional drugs, and is also an excellent drug that can maintain the antifungal effect for a long period of time. be.

Next, the present invention will be explained in more detail with reference to Examples.

Reference example 1 Minimum inhibitory concentration of TBZ, AMB and a mixture of both (
MIC) was measured by the following method.

Sterile trypto-soy agar plates containing drug at any concentration (
(for bacteria) and potato dextrose agar plates (for fungi)
A sterile physiological saline suspension of various bacterial strains (a bacterial suspension for bacteria, a spore suspension for mold) was applied to this using a platinum loop. In the case of bacteria, this is 30oo,
The cells were cultured for 4 hours, or for 2,500 and 120 hours in the case of fungi, and the presence or absence of growth at each concentration of the drug was observed to determine the MIC. Table 1 shows the composition of the culture medium used in the test and the types of test bacterial strains, and the results. Note that penzalkonium chloride was used as AMB. Composition of culture medium: Potato dextrose agar medium (medium LOOO0 medium) trypto-soy agar medium (medium 1000 medium) tryptone
15th evening soibepton
5 evenings Sodium chloride 5 evenings Cold weather 15 evenings Types of test bacterial strains; Bacteria; ■Snephylococcus aureus lAM
lol■ Escherichia coli
jacoli) lAM 1239■ Proteus vulgaris (Proteusvul chicks) lAM120
3■ Bacillus subtilis (Bacillus female sub)
ilis) lAM 1213 Mold; ■ Aspergillus niger
niger) ATCC6275 ■ Penicillium mcitrinum lAM
7316■ Trichoderma viride
rmaviride) LAM5141■ Fusarium・
Moniliforme (F order arlmmmonili phantom rme)
As is clear from Table 1 of IF○6349, TBZ and A
By mixing MB, an expansion of the antibacterial spectrum and a synergistic antibacterial effect against certain bacteria can be observed.

Reference Example 2 of Table 1 Regarding T and AMB alone and their mixtures in equal amounts, JIS-Z-2911 "Mold resistance test method,
7. Antifungal properties test was conducted in accordance with the section 7. Paint Test.

That is, the ethanol solution of each sample was coated with rotor paper (Toyo Rōshi,
No. 2 (diameter 30 side), and after leaving it indoors for 2 hours, place it in the center of the culture surface of a designated culture medium in a vetri dish, and sprinkle the test mold spore suspension lcc evenly on it. , then cover the petri dish and set the temperature to 28.
00 for 148 hours. The culture medium used at this time had a composition of 1000 parts of water, 40 parts of glucose, 10 parts of beptone, and 25 parts of agar. Note that penzalkonium chloride was used as AMB. The types of mold used in the test are shown below, and the results are shown in Table 2. Type of mold tested: Aspergillus niger
r) ATCC9642 Benicillium Luteum (Pen
ATCC 9644 Trichoderma T-1 (Tr to Hoderma T-1) A
As is clear from TCC9645 Table 2, TBZ,A
When using MB alone, mold growth is observed on the 3rd day even at high concentrations of 1.0% and 0.8%, but when both are used together, mold growth can be seen on the 5th day even at concentrations as low as 0.1%. It will be later.

Table 2 Each symbol in the table represents the following meaning.

- Bacterial growth is not observed in an area of 1/3 or less ++ Bacterial growth is in an area of 1'3 or more Example 1 Various blended compositions shown in Table 3 containing the blended composition according to the present invention was prepared, and a fungicidal power test was conducted in the same manner as in Reference Example 2.

The results are shown in Table 4. As is clear from Table 4, compositions A and B have significantly improved antifungal properties compared to the case where TBZ and penzalkonium chloride were each blended alone. This indicates that the compounded composition of the present invention can effectively prevent mold. Note that Liponox IGH (manufactured by Lion Corporation) was used as a nonionic surfactant. Table 3 Note: Tsukuda is a blended composition according to the present invention.

Table 4 Note: The Fuyuhashi Kogo in the table has the same meaning as in Table 2.

Example 2 A bacterial suspension of 0.1% in sterile physiological saline was added to the blended composition of Example 1, 9.9%, and the bactericidal power was determined by determining the change over time in the number of viable bacteria.

At this time, the number of viable bacteria was measured by the plate dilution method using a trypto-soy agar medium, and the determination was made after culturing at 30 qo for 4 hours. The results are shown in Table 5. Table 5■ Pseudomonas aeruginosa (Pseudo-
m0naS aeruglnosa) IF○ 3447
■ Stahylococcus aureus (Snephylo-
C0CCus aureus)lAM IOII■Escherichia-col.i
)lAM IO97 As can be seen from Table 5, the composition of the present invention has an excellent bactericidal effect that kills bacteria in a short time.

Example 3 The wall inside the refrigerator was divided into 8 cm long and 8 horizontal sections, and each section was divided into {1} no treatment, ■ water wipe treatment, and [3'
After wiping with a kitchen detergent at a concentration normally used, and after spraying the composition of Example 1 as an aerosol and wiping with gauze, sterilization was performed by dipping in 10 ml of sterile physiological saline. Bacteria were collected by wiping each section with gauze (8 x 8 skin, 6 layers), then the gauze was soaked in sterile physiological saline to suspend it, and this suspension was added to the test solution. The number of viable bacteria was measured by the plate pouring method, and the measured value was used as the number of bacteria remaining in each section.
The remaining bacteria rate was calculated based on the number of cells/average) = 100).

The results are shown in Table 6. Table 6 As can be seen from this table, the number of remaining bacteria in the compartments treated with the composition of the present invention was extremely small, and the bacteria were almost completely sterilized and washed.

Claims (1)

[Scope of Claims] 1. An antibacterial detergent composition comprising a nonionic surfactant, 2-(4-thiazolyl)benzimidazole, and a long-chain alkyldimethylbenzylammonium salt. 2. The antibacterial cleaning composition according to claim 1, wherein the long-chain alkyldimethylbenzylammonium salt is benzalkonium chloride.