JPS60258199A - システイニルグリシンの分離法 - Google Patents
システイニルグリシンの分離法Info
- Publication number
- JPS60258199A JPS60258199A JP11357184A JP11357184A JPS60258199A JP S60258199 A JPS60258199 A JP S60258199A JP 11357184 A JP11357184 A JP 11357184A JP 11357184 A JP11357184 A JP 11357184A JP S60258199 A JPS60258199 A JP S60258199A
- Authority
- JP
- Japan
- Prior art keywords
- glutathione
- cysteinylglycine
- cysteinyl glycine
- solution
- exchange resin
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- ZUKPVRWZDMRIEO-VKHMYHEASA-N L-cysteinylglycine Chemical compound SC[C@H]([NH3+])C(=O)NCC([O-])=O ZUKPVRWZDMRIEO-VKHMYHEASA-N 0.000 title claims abstract description 34
- 108010016616 cysteinylglycine Proteins 0.000 title claims abstract description 33
- 238000000926 separation method Methods 0.000 title claims abstract description 4
- RWSXRVCMGQZWBV-WDSKDSINSA-N glutathione Chemical compound OC(=O)[C@@H](N)CCC(=O)N[C@@H](CS)C(=O)NCC(O)=O RWSXRVCMGQZWBV-WDSKDSINSA-N 0.000 claims abstract description 53
- 108010024636 Glutathione Proteins 0.000 claims abstract description 26
- 229960003180 glutathione Drugs 0.000 claims abstract description 26
- 238000004132 cross linking Methods 0.000 claims abstract description 14
- NWUYHJFMYQTDRP-UHFFFAOYSA-N 1,2-bis(ethenyl)benzene;1-ethenyl-2-ethylbenzene;styrene Chemical compound C=CC1=CC=CC=C1.CCC1=CC=CC=C1C=C.C=CC1=CC=CC=C1C=C NWUYHJFMYQTDRP-UHFFFAOYSA-N 0.000 claims abstract description 9
- 239000003729 cation exchange resin Substances 0.000 claims abstract description 8
- 230000002378 acidificating effect Effects 0.000 claims description 6
- 238000001179 sorption measurement Methods 0.000 abstract description 7
- 239000011347 resin Substances 0.000 abstract description 6
- 229920005989 resin Polymers 0.000 abstract description 6
- 229940041514 candida albicans extract Drugs 0.000 abstract description 2
- 230000000694 effects Effects 0.000 abstract description 2
- 239000012138 yeast extract Substances 0.000 abstract description 2
- 239000002253 acid Substances 0.000 abstract 2
- 239000007788 liquid Substances 0.000 description 13
- 238000000034 method Methods 0.000 description 7
- 239000000126 substance Substances 0.000 description 7
- MYRTYDVEIRVNKP-UHFFFAOYSA-N 1,2-Divinylbenzene Chemical compound C=CC1=CC=CC=C1C=C MYRTYDVEIRVNKP-UHFFFAOYSA-N 0.000 description 4
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 4
- 238000004128 high performance liquid chromatography Methods 0.000 description 4
- 239000000243 solution Substances 0.000 description 4
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 3
- 238000003756 stirring Methods 0.000 description 3
- DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 description 2
- PPBRXRYQALVLMV-UHFFFAOYSA-N Styrene Chemical compound C=CC1=CC=CC=C1 PPBRXRYQALVLMV-UHFFFAOYSA-N 0.000 description 2
- 239000007864 aqueous solution Substances 0.000 description 2
- 239000003456 ion exchange resin Substances 0.000 description 2
- 229920003303 ion-exchange polymer Polymers 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- 239000004471 Glycine Substances 0.000 description 1
- 235000001014 amino acid Nutrition 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 229920001429 chelating resin Polymers 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 229920001577 copolymer Polymers 0.000 description 1
- 150000001879 copper Chemical class 0.000 description 1
- XUJNEKJLAYXESH-UHFFFAOYSA-N cysteine Natural products SCC(N)C(O)=O XUJNEKJLAYXESH-UHFFFAOYSA-N 0.000 description 1
- 235000018417 cysteine Nutrition 0.000 description 1
- 238000000354 decomposition reaction Methods 0.000 description 1
- IDGUHHHQCWSQLU-UHFFFAOYSA-N ethanol;hydrate Chemical compound O.CCO IDGUHHHQCWSQLU-UHFFFAOYSA-N 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000003960 organic solvent Substances 0.000 description 1
- 108090000765 processed proteins & peptides Proteins 0.000 description 1
- 102000004196 processed proteins & peptides Human genes 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
Landscapes
- Treatment Of Liquids With Adsorbents In General (AREA)
- Peptides Or Proteins (AREA)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP11357184A JPS60258199A (ja) | 1984-06-01 | 1984-06-01 | システイニルグリシンの分離法 |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP11357184A JPS60258199A (ja) | 1984-06-01 | 1984-06-01 | システイニルグリシンの分離法 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS60258199A true JPS60258199A (ja) | 1985-12-20 |
| JPH0557998B2 JPH0557998B2 (enrdf_load_stackoverflow) | 1993-08-25 |
Family
ID=14615616
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP11357184A Granted JPS60258199A (ja) | 1984-06-01 | 1984-06-01 | システイニルグリシンの分離法 |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS60258199A (enrdf_load_stackoverflow) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2017159555A1 (ja) * | 2016-03-17 | 2017-09-21 | 協和発酵バイオ株式会社 | 還元型グルタチオンの結晶及びその製造方法 |
-
1984
- 1984-06-01 JP JP11357184A patent/JPS60258199A/ja active Granted
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2017159555A1 (ja) * | 2016-03-17 | 2017-09-21 | 協和発酵バイオ株式会社 | 還元型グルタチオンの結晶及びその製造方法 |
| CN108779147A (zh) * | 2016-03-17 | 2018-11-09 | 协和发酵生化株式会社 | 还原型谷胱甘肽的晶体及其制造方法 |
| JPWO2017159555A1 (ja) * | 2016-03-17 | 2019-01-24 | 協和発酵バイオ株式会社 | 還元型グルタチオンの結晶及びその製造方法 |
| EP3431488A4 (en) * | 2016-03-17 | 2019-10-16 | Kyowa Hakko Bio Co., Ltd. | CRYSTAL WITH GLUTATHION WITH REDUCED FORM AND METHOD FOR THE PRODUCTION THEREOF |
| US10858393B2 (en) | 2016-03-17 | 2020-12-08 | Kyowa Hakko Bio Co., Ltd. | Crystal of reduced glutathione and method for producing same |
| JP2021191805A (ja) * | 2016-03-17 | 2021-12-16 | 協和発酵バイオ株式会社 | 還元型グルタチオンの結晶及びその製造方法 |
| CN108779147B (zh) * | 2016-03-17 | 2023-12-08 | 协和发酵生化株式会社 | 还原型谷胱甘肽的晶体及其制造方法 |
Also Published As
| Publication number | Publication date |
|---|---|
| JPH0557998B2 (enrdf_load_stackoverflow) | 1993-08-25 |
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